##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60045125	34.0	31.0	34.0	31.0	34.0
2	32.7555805	34.0	31.0	34.0	31.0	34.0
3	32.7924875	34.0	31.0	34.0	31.0	34.0
4	36.16758025	37.0	37.0	37.0	35.0	37.0
5	36.165372	37.0	37.0	37.0	35.0	37.0
6	36.18188925	37.0	37.0	37.0	35.0	37.0
7	36.18793475	37.0	37.0	37.0	35.0	37.0
8	36.16628125	37.0	37.0	37.0	35.0	37.0
9	37.92279	39.0	39.0	39.0	37.0	39.0
10-14	38.202027	39.4	39.2	39.4	35.4	39.4
15-19	39.38168785	41.0	40.0	41.0	37.0	41.0
20-24	39.16553785	41.0	39.2	41.0	36.0	41.0
25-29	38.804568399999994	40.4	38.6	41.0	35.0	41.0
30-34	38.184286	40.0	37.4	41.0	34.2	41.0
35-39	37.23026635	39.0	35.4	41.0	33.0	41.0
40-44	36.31635970000001	37.2	35.0	40.0	32.0	41.0
45-49	35.342296399999995	35.2	35.0	39.0	31.2	41.0
50-54	33.96386305	35.0	33.6	36.2	29.4	39.2
55-59	33.9963369	35.0	35.0	35.0	31.0	38.2
60-64	33.628285749999996	35.0	34.6	35.0	31.0	36.4
65-69	33.31132055	35.0	34.0	35.0	30.0	35.2
70-74	33.0444291	35.0	34.0	35.0	29.6	35.0
75-79	32.800044150000005	35.0	33.4	35.0	29.0	35.0
80-84	32.5291668	35.0	33.0	35.0	28.2	35.0
85-89	32.2482675	35.0	33.0	35.0	27.0	35.0
90-94	31.88945745	35.0	33.0	35.0	26.6	35.0
95-99	31.331771300000003	35.0	33.0	35.0	24.2	35.0
100-101	30.245039	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8246.0
3	1176.0
4	1530.0
5	1753.0
6	2319.0
7	2911.0
8	3461.0
9	4076.0
10	4318.0
11	4687.0
12	4675.0
13	4767.0
14	4843.0
15	4822.0
16	5301.0
17	5867.0
18	6652.0
19	7371.0
20	8546.0
21	10455.0
22	12440.0
23	15233.0
24	18056.0
25	22072.0
26	27385.0
27	33771.0
28	41115.0
29	51586.0
30	64754.0
31	82319.0
32	109852.0
33	155256.0
34	255170.0
35	613165.0
36	1418958.0
37	851180.0
38	119040.0
39	10792.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.284114051677555	24.44924401718727	13.569610270072422	23.69703166106276
2	30.492128040618994	24.88840322989949	19.12001697468352	25.49945175479799
3	29.372307228295437	19.991623114292636	21.45574942690729	29.18032023050464
4	30.59176942198194	19.879609210857215	19.918934597623643	29.609686769537202
5	31.095187457805867	21.113962672518422	20.109319595737936	27.68153027393777
6	29.335235123824532	19.46746529186469	17.507751781802327	33.68954780250845
7	32.59637782706165	19.05376294567291	19.457072012217907	28.892787215047527
8	28.355545741236465	19.854793143195923	19.909422847935137	31.88023826763248
9	30.528485649186532	18.210892940895718	19.273378245018677	31.98724316489907
10-14	30.433710335602697	19.67166236336218	19.546789547016253	30.34783775401887
15-19	30.666479310420925	19.193151128383516	19.364574086559486	30.775795474636077
20-24	30.593939936025393	19.250115519572443	19.35782873528667	30.798115809115494
25-29	30.7165988180525	19.377988396221234	19.290109175725124	30.615303610001142
30-34	30.881500066182628	19.143459167069725	19.09243473754772	30.882606029199927
35-39	30.706412452949515	19.205806341648664	19.238994756240498	30.84878644916132
40-44	30.847581915510382	19.280403254814424	19.165301624886517	30.706713204788677
45-49	30.98762511546908	19.038432011155052	19.012910030893448	30.961032842482417
50-54	30.7653089732443	19.134332567599333	19.17000890557831	30.93034955357806
55-59	30.881003487348817	19.23434281816318	19.157250211300667	30.727403483187327
60-64	30.98348809834881	19.031351903135192	18.99170189917019	30.993458099345812
65-69	30.77851461677719	19.13116786967518	19.16555287483293	30.9247646387147
70-74	30.9044	19.23781	19.100195	30.757595
75-79	30.990076251791365	19.04355725889563	18.996780853264642	30.969585636048365
80-84	30.804065043699598	19.117837010700182	19.159797622932068	30.91830032266816
85-89	30.897917724479427	19.21479980369995	19.124739781184946	30.762542690635676
90-94	31.028627402960694	19.030450206332883	18.979030111205706	30.96189227950072
95-99	30.856134714931606	19.10766428230062	19.138990150027155	30.89721085274062
100-101	31.017915924315982	19.346725564682394	19.075466499586717	30.559892011414906
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.981145712740314
2	55.99157979541699
3	58.552627458800075
4	60.20145619151914
5	58.776717731743645
6	63.024782926332975
7	61.489165042109185
8	60.23578400886894
9	62.515728814085605
10-14	60.78154808962157
15-19	61.442274785056995
20-24	61.39205574514088
25-29	61.33190242805364
30-34	61.76410609538255
35-39	61.55519890211084
40-44	61.55429512029906
45-49	61.948657957951504
50-54	61.69565852682236
55-59	61.608406970536144
60-64	61.976946197694616
65-69	61.703279255491886
70-74	61.661995000000005
75-79	61.95966188783974
80-84	61.72236536636775
85-89	61.660460415115104
90-94	61.99051968246141
95-99	61.75334556767223
100-101	61.57780793573089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	80.5
2	35.5
3	32.0
4	29.5
5	24.5
6	25.5
7	35.0
8	37.5
9	38.5
10	43.5
11	56.0
12	80.5
13	103.0
14	122.0
15	154.0
16	202.0
17	241.5
18	262.0
19	326.0
20	413.0
21	489.5
22	549.0
23	642.5
24	775.5
25	904.5
26	1068.5
27	1299.0
28	1495.0
29	1764.0
30	2162.0
31	2514.5
32	2840.5
33	3388.0
34	3989.5
35	4404.5
36	4962.0
37	5658.0
38	6442.0
39	7502.5
40	8569.0
41	9831.5
42	11436.5
43	13516.5
44	16421.0
45	19939.0
46	24555.0
47	30389.0
48	37171.5
49	45728.0
50	55991.5
51	67210.5
52	79790.5
53	93462.0
54	107875.5
55	121837.5
56	135739.5
57	148995.0
58	160711.0
59	171329.0
60	180625.0
61	188138.0
62	193303.5
63	196402.0
64	197388.0
65	195562.0
66	190390.0
67	182891.5
68	172254.5
69	158218.5
70	142022.0
71	124892.5
72	107201.0
73	89020.0
74	72003.0
75	56504.5
76	42735.5
77	31501.0
78	22460.5
79	15244.5
80	9810.0
81	6128.5
82	3711.0
83	2168.5
84	1236.0
85	715.0
86	455.0
87	298.5
88	228.0
89	176.5
90	123.0
91	96.0
92	92.0
93	67.5
94	50.5
95	42.0
96	29.0
97	23.5
98	16.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21102500000000002
2	0.20432499999999998
3	0.201575
4	0.1917
5	0.20087500000000003
6	0.111675
7	0.113825
8	0.1459
9	0.163325
10-14	0.090345
15-19	0.083395
20-24	0.049405
25-29	0.04463
30-34	0.087075
35-39	0.05548499999999999
40-44	0.02748
45-49	0.02735
50-54	0.045795
55-59	0.035809999999999995
60-64	9.999999999999999E-6
65-69	1.4999999999999999E-5
70-74	0.0
75-79	0.003005
80-84	0.013375
85-89	2.4999999999999998E-5
90-94	1.85E-4
95-99	0.00277
100-101	0.007949999999999999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.21441316266912036
#Duplication Level	Relative count
1	100.0
2	0.1717989301384409
3	0.002504357582193016
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	335595	3.1998694	4.0294843	1
TTTTT	317495	3.1810677	3.7862315	10-14
>>END_MODULE