##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5716065	34.0	31.0	34.0	31.0	34.0
2	32.714892	34.0	31.0	34.0	31.0	34.0
3	32.7738565	34.0	31.0	34.0	31.0	34.0
4	36.1789785	37.0	37.0	37.0	35.0	37.0
5	36.18702925	37.0	37.0	37.0	35.0	37.0
6	36.18986675	37.0	37.0	37.0	35.0	37.0
7	36.17795975	37.0	37.0	37.0	35.0	37.0
8	36.146968	37.0	37.0	37.0	35.0	37.0
9	37.9073665	39.0	39.0	39.0	35.0	39.0
10-14	38.18970975	39.4	38.6	39.4	35.4	39.4
15-19	39.360326199999996	41.0	40.0	41.0	36.6	41.0
20-24	39.1834008	41.0	39.0	41.0	36.0	41.0
25-29	38.8125435	40.4	38.6	41.0	35.0	41.0
30-34	38.2015976	40.0	37.4	41.0	34.2	41.0
35-39	37.281566	39.0	35.2	41.0	33.0	41.0
40-44	36.39180220000001	37.2	35.0	40.0	32.2	41.0
45-49	35.4217902	35.2	35.0	39.0	31.4	41.0
50-54	34.036853050000005	34.8	33.6	36.2	29.6	39.2
55-59	34.1292955	35.0	35.0	35.0	31.0	38.2
60-64	33.7194709	35.0	34.8	35.0	31.0	36.4
65-69	33.39526355	35.0	34.0	35.0	30.2	35.0
70-74	33.1678051	35.0	34.0	35.0	30.0	35.0
75-79	32.97078595	35.0	34.0	35.0	29.2	35.0
80-84	32.72377655	35.0	33.0	35.0	29.0	35.0
85-89	32.45961045	35.0	33.0	35.0	28.2	35.0
90-94	32.1391386	35.0	33.0	35.0	27.0	35.0
95-99	31.675983300000002	35.0	33.0	35.0	25.8	35.0
100-101	30.624353625	34.0	31.5	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6118.0
3	954.0
4	1140.0
5	1431.0
6	1816.0
7	2387.0
8	2853.0
9	3394.0
10	3828.0
11	3935.0
12	4043.0
13	4168.0
14	4266.0
15	4432.0
16	4733.0
17	5353.0
18	5862.0
19	6716.0
20	7903.0
21	9484.0
22	11640.0
23	14178.0
24	17634.0
25	21826.0
26	26484.0
27	32971.0
28	40965.0
29	50318.0
30	63945.0
31	82262.0
32	109037.0
33	153521.0
34	250030.0
35	612950.0
36	1447760.0
37	852148.0
38	116753.0
39	10675.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32588943864492	24.40457183925592	13.558415221179764	23.711123500919392
2	30.497431985914208	24.861939839102483	19.09396799466678	25.54666018031653
3	29.406491967995102	19.971482466321234	21.41497615948198	29.207049406201683
4	30.628418841806116	19.843707229291283	19.910864279205185	29.61700964969742
5	31.109398882771007	21.062393211570548	20.122730081378098	27.705477824280344
6	29.38384889477501	19.425211933016303	17.49417769188841	33.696761480320276
7	32.60930565182141	18.984043356054162	19.497758830822328	28.908892161302102
8	28.326713848183292	19.858380328297418	19.855100008438686	31.959805815080607
9	30.53561666374084	18.206974754161664	19.232575197718706	32.02483338437879
10-14	30.442846115212046	19.644663561507194	19.518907506030775	30.393582817249982
15-19	30.686762904044524	19.153135897124464	19.352905200062313	30.807195998768695
20-24	30.602902015890265	19.2031773329862	19.346434075283355	30.847486575840172
25-29	30.735970369712206	19.34445209562684	19.282520543624848	30.63705699103611
30-34	30.89956152332798	19.100746244568583	19.080278428357964	30.91941380374547
35-39	30.727362035724763	19.175929591101188	19.2117762281313	30.884932145042747
40-44	30.85717630156694	19.239684610505382	19.150182186356332	30.752956901571345
45-49	31.004294319330178	19.019694167796565	19.001502731666463	30.97450878120679
50-54	30.79366313342302	19.10544422079441	19.154229677045482	30.946662968737087
55-59	30.902046593923917	19.19540049347313	19.130162317104755	30.772390595498194
60-64	31.012096643547913	19.01606845784645	18.964948273813786	31.006886624791854
65-69	30.80274	19.08334	19.14544	30.96848
70-74	30.927375	19.208765	19.0885	30.775360000000003
75-79	31.03193265881133	18.999023145720805	18.966794705334795	31.00224949013307
80-84	30.80611640603035	19.091364720244997	19.147555064887445	30.954963808837206
85-89	30.92932132596528	19.179881415023516	19.089279140906438	30.801518118104767
90-94	31.043851125355914	19.03233961369425	18.94175873505973	30.982050525890102
95-99	30.83152321358246	19.0833038488226	19.12919716698652	30.955975770608426
100-101	31.006090056017317	19.31356506125874	19.093993994331885	30.58635088839206
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.03701293956432
2	56.04409216623074
3	58.613541374196785
4	60.245428491503525
5	58.814876707051354
6	63.08061037509529
7	61.51819781312351
8	60.286519663263896
9	62.560450048119634
10-14	60.83642893246203
15-19	61.49395890281322
20-24	61.450388591730444
25-29	61.37302736074831
30-34	61.81897532707345
35-39	61.61229418076751
40-44	61.61013320313828
45-49	61.97880310053697
50-54	61.740326102160104
55-59	61.674437189422115
60-64	62.01898326833977
65-69	61.77122000000001
70-74	61.702735
75-79	62.034182148944396
80-84	61.76108021486756
85-89	61.73083944407005
90-94	62.025901651246016
95-99	61.78749898419088
100-101	61.592440944409375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	7.5
2	6.0
3	6.5
4	7.5
5	7.5
6	13.5
7	17.0
8	16.0
9	26.5
10	43.0
11	61.0
12	84.5
13	122.5
14	147.0
15	175.5
16	208.0
17	232.5
18	281.0
19	338.0
20	404.0
21	453.0
22	533.0
23	634.5
24	717.0
25	825.0
26	1032.0
27	1234.0
28	1437.5
29	1704.5
30	2033.0
31	2439.0
32	2844.0
33	3326.5
34	3817.5
35	4343.0
36	4948.5
37	5588.5
38	6251.5
39	7097.5
40	8146.0
41	9533.5
42	11435.0
43	13478.5
44	16138.5
45	19861.5
46	24140.0
47	29733.5
48	36965.5
49	45182.0
50	55205.0
51	66821.0
52	79314.5
53	92668.5
54	106626.0
55	121080.0
56	135291.5
57	148549.0
58	160890.0
59	171726.0
60	180699.0
61	188196.5
62	193919.5
63	197045.5
64	198165.5
65	196497.0
66	191490.5
67	183603.5
68	172696.5
69	158871.0
70	142659.0
71	125305.5
72	107450.0
73	89874.5
74	72730.0
75	57060.5
76	43533.5
77	31942.0
78	22444.5
79	15116.5
80	9884.5
81	6124.0
82	3545.5
83	1985.5
84	1094.5
85	621.0
86	357.5
87	220.0
88	150.0
89	112.5
90	90.5
91	68.5
92	46.0
93	37.0
94	32.5
95	22.0
96	19.5
97	15.5
98	6.5
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09709999999999999
2	0.0979
3	0.09655
4	0.08495
5	0.06525
6	0.10405
7	0.12467500000000001
8	0.16217499999999999
9	0.170675
10-14	0.10819
15-19	0.089755
20-24	0.05706
25-29	0.05902
30-34	0.08704500000000001
35-39	0.060360000000000004
40-44	0.0474
45-49	0.035379999999999995
50-54	0.05218
55-59	0.04319
60-64	3.5999999999999997E-4
65-69	0.0
70-74	0.0
75-79	0.010675
80-84	0.01841
85-89	0.00251
90-94	9.7E-4
95-99	0.018125
100-101	0.015287499999999999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2722617709504091
#Duplication Level	Relative count
1	100.0
2	0.17732805690527476
3	0.0035064870009517608
4	0.0015027801432650402
5	0.0
6	0.0
7	0.0
8	5.009267144216801E-4
9	0.0
10++	0.0025046335721084006
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	304190	3.0668755	3.7253473	9
>>END_MODULE