##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.614769	34.0	31.0	34.0	31.0	34.0
2	32.7663715	34.0	31.0	34.0	31.0	34.0
3	32.82351725	34.0	31.0	34.0	31.0	34.0
4	36.2133625	37.0	37.0	37.0	35.0	37.0
5	36.231927	37.0	37.0	37.0	35.0	37.0
6	36.19804225	37.0	37.0	37.0	35.0	37.0
7	36.19361	37.0	37.0	37.0	35.0	37.0
8	36.15814025	37.0	37.0	37.0	35.0	37.0
9	37.91621175	39.0	39.0	39.0	37.0	39.0
10-14	38.2039386	39.4	38.8	39.4	35.4	39.4
15-19	39.3838643	41.0	40.0	41.0	37.0	41.0
20-24	39.19044705	41.0	39.2	41.0	36.0	41.0
25-29	38.8122636	40.4	38.6	41.0	35.0	41.0
30-34	38.17757815	40.0	37.4	41.0	34.0	41.0
35-39	37.26293295000001	39.0	35.2	40.8	33.0	41.0
40-44	36.3468199	37.2	35.0	40.0	32.0	41.0
45-49	35.35943555	35.2	35.0	39.0	31.2	41.0
50-54	33.9694262	34.8	33.4	36.2	29.6	39.2
55-59	34.07774305	35.0	35.0	35.0	31.0	38.2
60-64	33.6615192	35.0	34.6	35.0	31.0	36.4
65-69	33.3244009	35.0	34.0	35.0	30.2	35.0
70-74	33.067553950000004	35.0	34.0	35.0	29.6	35.0
75-79	32.8417696	35.0	33.6	35.0	29.0	35.0
80-84	32.55876225	35.0	33.0	35.0	28.4	35.0
85-89	32.25727525	35.0	33.0	35.0	27.0	35.0
90-94	31.8703303	35.0	33.0	35.0	26.6	35.0
95-99	31.33123655	35.0	33.0	35.0	24.2	35.0
100-101	30.205691375	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6620.0
3	1089.0
4	1453.0
5	1754.0
6	2175.0
7	2778.0
8	3253.0
9	3754.0
10	3978.0
11	4376.0
12	4470.0
13	4513.0
14	4684.0
15	4744.0
16	5185.0
17	5590.0
18	6403.0
19	7326.0
20	8377.0
21	10255.0
22	12005.0
23	14944.0
24	18163.0
25	22504.0
26	27383.0
27	33743.0
28	41393.0
29	51380.0
30	65107.0
31	83287.0
32	111157.0
33	157267.0
34	258491.0
35	626605.0
36	1428052.0
37	830976.0
38	114535.0
39	10148.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.31302364627255	24.440079873811793	13.541654673413925	23.705241806501736
2	30.48765719072667	24.840400963023868	19.12040750862363	25.551534337625835
3	29.379572087623966	19.94408418145246	21.433196233250275	29.2431474976733
4	30.616070872403707	19.814698673956773	19.919242123780545	29.649988329858974
5	31.1613017979738	21.04988501083097	20.10613076268602	27.68268242850921
6	29.326377488624217	19.439414138520604	17.53315077329268	33.701057599562496
7	32.57602167826214	18.981727749477827	19.494582938887138	28.9476676333729
8	28.35435027048262	19.811022943420127	19.897734720588147	31.93689206550911
9	30.55148679517493	18.193503787119784	19.252594878371337	32.00241453933395
10-14	30.446020890798266	19.633448605695683	19.53566425500645	30.384866248499602
15-19	30.675093375476887	19.166435422603634	19.340717963397463	30.817753238522016
20-24	30.616121270842438	19.196863573304427	19.339931968833877	30.84708318701926
25-29	30.732617809602402	19.360637629247517	19.26323450228093	30.643510058869154
30-34	30.904480912038657	19.123597863090094	19.07205003687779	30.899871187993455
35-39	30.742365786178727	19.181241921200495	19.193529125802915	30.882863166817863
40-44	30.855985578118343	19.24275016615198	19.14580024925548	30.7554640064742
45-49	30.96314793212786	19.034933812175307	19.00689276540493	30.9950254902919
50-54	30.793203636064913	19.091166882179504	19.162759902425368	30.952869579330223
55-59	30.884369369907983	19.19973352033871	19.123803026583378	30.79209408316993
60-64	31.018497199017574	19.00495536313171	18.962195249817412	31.0143521880333
65-69	30.79838541814323	19.119576987513536	19.137037007592557	30.945000586750677
70-74	30.920563914253762	19.19995363997914	19.10773359848012	30.771748847286982
75-79	31.00180562505535	19.026769645317113	18.961958361863182	31.009466367764354
80-84	30.82400954260036	19.09662382999367	19.11289326341428	30.966473363991685
85-89	30.953571572681604	19.188103909587298	19.08632676717845	30.77199775055265
90-94	31.05506837414912	19.0134150264076	18.92379884795485	31.007717751488435
95-99	30.84908031366339	19.085877820120942	19.10148246640437	30.96355939981129
100-101	31.03744227683889	19.31726988466557	19.06134204403969	30.583945794455854
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.01826545277428
2	56.039191528352504
3	58.62271958529727
4	60.26605920226268
5	58.843984226483
6	63.027435088186714
7	61.52368931163503
8	60.291242335991726
9	62.55390133450888
10-14	60.83088713929787
15-19	61.4928466139989
20-24	61.4632044578617
25-29	61.376127868471556
30-34	61.80435210003211
35-39	61.62522895299659
40-44	61.611449584592535
45-49	61.95817342241976
50-54	61.746073215395135
55-59	61.676463453077915
60-64	62.032849387050874
65-69	61.74338600489391
70-74	61.69231276154075
75-79	62.01127199281971
80-84	61.79048290659205
85-89	61.725569323234254
90-94	62.06278612563755
95-99	61.81263971347468
100-101	61.62138807129474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	7.0
2	5.5
3	6.0
4	7.0
5	7.5
6	9.0
7	14.5
8	22.0
9	28.5
10	44.5
11	61.5
12	75.0
13	103.5
14	145.5
15	176.5
16	205.0
17	261.0
18	323.0
19	365.5
20	399.0
21	446.0
22	535.5
23	632.0
24	744.5
25	869.5
26	1008.0
27	1205.0
28	1436.5
29	1697.0
30	2033.0
31	2415.5
32	2850.0
33	3288.5
34	3724.0
35	4231.0
36	4834.0
37	5549.0
38	6360.5
39	7266.0
40	8350.5
41	9730.5
42	11453.0
43	13502.0
44	16187.0
45	19582.0
46	23911.5
47	29553.5
48	36770.5
49	45394.5
50	55308.0
51	66527.0
52	79250.5
53	92872.5
54	106750.0
55	121826.5
56	136236.0
57	148859.5
58	160759.0
59	171495.0
60	180784.0
61	188506.5
62	194009.5
63	197301.0
64	198253.5
65	196546.0
66	191414.5
67	183019.5
68	172045.0
69	158349.5
70	142623.5
71	125267.5
72	107180.0
73	89426.5
74	72399.5
75	56729.5
76	43003.0
77	31582.5
78	22440.5
79	15338.0
80	10093.5
81	6295.5
82	3746.0
83	2228.5
84	1286.0
85	704.5
86	436.5
87	312.5
88	220.5
89	175.5
90	138.5
91	107.0
92	82.5
93	58.0
94	44.5
95	37.0
96	31.0
97	26.5
98	19.0
99	10.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11745
2	0.08555
3	0.073
4	0.06547499999999999
5	0.066675
6	0.16069999999999998
7	0.17650000000000002
8	0.2154
9	0.188
10-14	0.122055
15-19	0.087525
20-24	0.07227
25-29	0.06480999999999999
30-34	0.10248
35-39	0.058635
40-44	0.046329999999999996
45-49	0.039395
50-54	0.060090000000000005
55-59	0.059915
60-64	2.65E-4
65-69	1.15E-4
70-74	4.4999999999999996E-5
75-79	0.009695
80-84	0.02725
85-89	0.002105
90-94	0.001315
95-99	0.029775
100-101	0.0157625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2308695586993359
#Duplication Level	Relative count
1	100.0
2	0.16929542050878785
3	0.0015026220755217856
4	0.001001748050347857
5	0.0
6	0.0
7	0.0
8	5.008740251739285E-4
9	0.0
10++	0.0025043701258696425
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	308490	3.1142516	3.7625468	10-14
AAAAA	314980	3.0310578	4.1976314	1
>>END_MODULE