##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62731725	34.0	31.0	34.0	31.0	34.0
2	32.799405	34.0	31.0	34.0	31.0	34.0
3	32.83967725	34.0	31.0	34.0	31.0	34.0
4	36.2332555	37.0	37.0	37.0	35.0	37.0
5	36.230728	37.0	37.0	37.0	35.0	37.0
6	36.2085465	37.0	37.0	37.0	35.0	37.0
7	36.21136425	37.0	37.0	37.0	35.0	37.0
8	36.17868025	37.0	37.0	37.0	35.0	37.0
9	37.9462195	39.0	39.0	39.0	37.0	39.0
10-14	38.2424831	39.4	39.2	39.4	37.0	39.4
15-19	39.4114012	41.0	40.0	41.0	37.0	41.0
20-24	39.21317485	41.0	39.2	41.0	36.0	41.0
25-29	38.843240800000004	40.4	38.6	41.0	35.0	41.0
30-34	38.212976000000005	40.0	37.4	41.0	34.2	41.0
35-39	37.28421355	39.0	35.4	41.0	33.0	41.0
40-44	36.35564245	37.2	35.0	40.0	32.0	41.0
45-49	35.35687345	35.2	35.0	39.0	31.4	41.0
50-54	33.94484860000001	34.8	33.4	36.2	29.6	39.2
55-59	34.07231805	35.0	35.0	35.0	31.0	38.2
60-64	33.65223475	35.0	34.6	35.0	31.0	36.4
65-69	33.295266000000005	35.0	34.0	35.0	30.0	35.0
70-74	33.026317649999996	35.0	34.0	35.0	29.4	35.0
75-79	32.76680475	35.0	33.2	35.0	29.0	35.0
80-84	32.482086800000005	35.0	33.0	35.0	27.8	35.0
85-89	32.17738945	35.0	33.0	35.0	27.0	35.0
90-94	31.784373700000003	35.0	33.0	35.0	25.8	35.0
95-99	31.2075228	35.0	33.0	35.0	23.8	35.0
100-101	30.0807215	34.0	30.5	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6653.0
3	1137.0
4	1428.0
5	1732.0
6	2192.0
7	2720.0
8	3243.0
9	3877.0
10	4047.0
11	4344.0
12	4517.0
13	4544.0
14	4731.0
15	4942.0
16	5391.0
17	5786.0
18	6810.0
19	7518.0
20	8705.0
21	10557.0
22	12346.0
23	15141.0
24	18437.0
25	22366.0
26	27552.0
27	33804.0
28	41827.0
29	51966.0
30	65576.0
31	84283.0
32	112689.0
33	159625.0
34	262126.0
35	624126.0
36	1411690.0
37	835561.0
38	115535.0
39	10396.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.335957097266835	24.41552310359942	13.575994575079156	23.67252522405459
2	30.502504075456304	24.87263182521641	19.127883627737873	25.49698047158941
3	29.35897567408594	19.907874316569387	21.45346016731337	29.2796898420313
4	30.613617748994205	19.855544008993977	19.91878603331914	29.612052208692674
5	31.12586377122429	21.05107240490823	20.13659707790242	27.686466745965067
6	29.333689570959855	19.43514968615949	17.536556716277012	33.69460402660364
7	32.61390214637247	18.99300337121452	19.448935028298816	28.944159454114192
8	28.31057331401512	19.880147932610704	19.889366166276893	31.919912587097276
9	30.5556612930854	18.17162336380912	19.263001625797997	32.009713717307484
10-14	30.450391592631536	19.66041082187601	19.507081423556745	30.382116161935706
15-19	30.665039049600257	19.180506782193653	19.358167352684877	30.796286815521213
20-24	30.607556358190052	19.23493529359732	19.35662627744764	30.800882070764985
25-29	30.72454030852046	19.36542939974336	19.277435783270256	30.632594508465928
30-34	30.896470240732537	19.123716213338515	19.086861608707494	30.89295193722145
35-39	30.697416849569475	19.21137561671075	19.216648220058655	30.87455931366112
40-44	30.85127268567512	19.263383343486353	19.148409693740227	30.7369342770983
45-49	30.98063755480881	19.040184591351753	19.001336258603953	30.977841595235482
50-54	30.787627344451767	19.120542734716302	19.156474333318275	30.93535558751365
55-59	30.883396723521972	19.196463257229585	19.13127950222805	30.788860517020396
60-64	31.001142352113355	19.02038018770335	18.98294511844847	30.995532341734833
65-69	30.83554	19.098045	19.129175	30.93724
70-74	30.89088	19.21503	19.0972	30.796889999999998
75-79	30.998287051248504	19.0186464249664	18.981349274785693	31.001717248999405
80-84	30.812834995370647	19.101772176866703	19.15206414887064	30.933328678892007
85-89	30.928163248571373	19.20634222219778	19.07825677389871	30.787237755332143
90-94	31.035255517627757	19.010719505359752	18.96092448046224	30.993100496550248
95-99	30.85666982727229	19.09631118363772	19.099957074511156	30.94706191457883
100-101	31.044592443354663	19.312707662481476	19.045429531458186	30.597270362705675
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.008482321321424
2	55.99948454704572
3	58.638665516117236
4	60.225669957686875
5	58.81233051718935
6	63.02829359756349
7	61.55806160048666
8	60.230485901112395
9	62.565375010392884
10-14	60.832507754567246
15-19	61.46132586512147
20-24	61.40843842895504
25-29	61.35713481698638
30-34	61.78942217795399
35-39	61.57197616323059
40-44	61.58820696277342
45-49	61.9584791500443
50-54	61.72298293196542
55-59	61.67225724054236
60-64	61.996674693848185
65-69	61.772780000000004
70-74	61.68777
75-79	62.000004300247916
80-84	61.746163674262654
85-89	61.715401003903516
90-94	62.02835601417801
95-99	61.803731741851124
100-101	61.64186280606033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	7.5
2	9.0
3	7.0
4	4.5
5	9.0
6	12.5
7	14.0
8	19.0
9	28.0
10	36.5
11	51.5
12	73.5
13	89.5
14	112.5
15	155.5
16	203.0
17	238.5
18	286.5
19	329.0
20	376.0
21	453.0
22	562.0
23	658.5
24	754.5
25	906.0
26	1093.0
27	1262.0
28	1474.0
29	1739.5
30	2034.5
31	2360.5
32	2749.5
33	3215.0
34	3709.5
35	4245.0
36	4926.0
37	5652.5
38	6375.0
39	7239.5
40	8309.0
41	9724.0
42	11405.0
43	13467.5
44	16164.0
45	19651.0
46	24226.0
47	30042.0
48	37022.5
49	45625.5
50	55605.0
51	66838.0
52	79561.0
53	93230.5
54	107482.0
55	121711.5
56	135562.5
57	148735.5
58	160839.0
59	171901.5
60	181406.5
61	188712.5
62	193943.0
63	197055.0
64	198098.5
65	196181.0
66	190805.5
67	182978.0
68	172323.0
69	158035.5
70	142013.0
71	124957.0
72	106965.0
73	89150.0
74	71993.0
75	56467.0
76	42824.5
77	31446.0
78	22460.5
79	15416.5
80	9994.0
81	6160.5
82	3709.5
83	2201.5
84	1308.5
85	824.0
86	539.0
87	326.0
88	246.5
89	208.0
90	149.5
91	123.0
92	101.5
93	79.0
94	63.5
95	43.0
96	30.5
97	29.0
98	25.0
99	16.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.127575
2	0.087875
3	0.076725
4	0.06645000000000001
5	0.08205000000000001
6	0.160275
7	0.176925
8	0.1978
9	0.1721
10-14	0.11048
15-19	0.07068
20-24	0.06244
25-29	0.05525000000000001
30-34	0.093895
35-39	0.049375
40-44	0.037965
45-49	0.034319999999999996
50-54	0.046195
55-59	0.059455
60-64	1.85E-4
65-69	0.0
70-74	0.0
75-79	0.005765
80-84	0.023805
85-89	3.5E-4
90-94	4.9999999999999996E-5
95-99	0.024435000000000002
100-101	0.010525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2084270505323191
#Duplication Level	Relative count
1	100.0
2	0.17229893865857263
3	0.0
4	5.008690077284088E-4
5	0.0010017380154568175
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	309185	3.121207	3.7633111	10-14
AAAAA	313640	3.011606	3.723337	1
>>END_MODULE