##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6295255	34.0	31.0	34.0	31.0	34.0
2	32.77595125	34.0	31.0	34.0	31.0	34.0
3	32.823132	34.0	31.0	34.0	31.0	34.0
4	36.19823225	37.0	37.0	37.0	35.0	37.0
5	36.206161	37.0	37.0	37.0	35.0	37.0
6	36.207219	37.0	37.0	37.0	35.0	37.0
7	36.21108025	37.0	37.0	37.0	35.0	37.0
8	36.19379375	37.0	37.0	37.0	35.0	37.0
9	37.97233125	39.0	39.0	39.0	37.0	39.0
10-14	38.2531803	39.4	39.2	39.4	37.0	39.4
15-19	39.447444250000004	41.0	40.0	41.0	37.0	41.0
20-24	39.258820199999995	41.0	39.4	41.0	36.2	41.0
25-29	38.8960747	40.6	38.6	41.0	35.0	41.0
30-34	38.284433650000004	40.0	37.6	41.0	34.4	41.0
35-39	37.36479545	39.2	35.4	41.0	33.0	41.0
40-44	36.4245157	37.4	35.0	40.0	32.6	41.0
45-49	35.44472535	35.2	35.0	39.0	31.4	41.0
50-54	34.035222000000005	34.8	33.6	36.2	29.6	39.4
55-59	34.14705105	35.0	35.0	35.2	31.0	38.2
60-64	33.753723349999994	35.0	35.0	35.0	31.0	36.4
65-69	33.4217996	35.0	34.0	35.0	30.6	35.2
70-74	33.1734305	35.0	34.0	35.0	30.0	35.0
75-79	32.928925299999996	35.0	34.0	35.0	29.2	35.0
80-84	32.6458351	35.0	33.0	35.0	29.0	35.0
85-89	32.36721899999999	35.0	33.0	35.0	27.4	35.0
90-94	32.02510575	35.0	33.0	35.0	27.0	35.0
95-99	31.508979850000003	35.0	33.0	35.0	24.6	35.0
100-101	30.495862250000002	34.0	31.5	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6577.0
3	1000.0
4	1247.0
5	1521.0
6	1922.0
7	2411.0
8	2866.0
9	3318.0
10	3652.0
11	4049.0
12	4083.0
13	4250.0
14	4297.0
15	4427.0
16	4873.0
17	5120.0
18	6082.0
19	6851.0
20	8160.0
21	9590.0
22	11484.0
23	14104.0
24	17315.0
25	21232.0
26	26441.0
27	32035.0
28	40182.0
29	50506.0
30	64001.0
31	81628.0
32	109226.0
33	154929.0
34	252387.0
35	602988.0
36	1427494.0
37	873199.0
38	123223.0
39	11234.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.256629905669264	24.480725026002577	13.592333698738852	23.67031136958931
2	30.4373698453108	24.895682151448405	19.14559668607221	25.521351317168584
3	29.33379414147561	19.952967516782557	21.459058824413315	29.254179517328517
4	30.617366060730205	19.91597204710834	19.921130516024686	29.545531376136775
5	31.084996691732037	21.105898133395577	20.12536357138671	27.68374160348568
6	29.325872491111515	19.457058215652793	17.551133035911253	33.66593625732443
7	32.54406364779736	19.018631225748024	19.480667792978366	28.956637333476248
8	28.35727868847533	19.824244916234246	19.906580811629595	31.911895583660833
9	30.532112719931526	18.182041277086554	19.26172221078669	32.024123792195226
10-14	30.44310777695175	19.656731772886676	19.53476461886203	30.365395831299548
15-19	30.658106645883155	19.184415608332607	19.39106636575012	30.766411380034114
20-24	30.560526594780512	19.226357036172274	19.37707671073585	30.83603965831137
25-29	30.7157407333296	19.36471981878867	19.296955665655492	30.622583782226243
30-34	30.88626295423654	19.131167838061284	19.123846204661422	30.858723003040755
35-39	30.712133808905683	19.185551162867252	19.23134803189031	30.87096699633675
40-44	30.84473465915176	19.26503711190768	19.166227761330674	30.724000467609887
45-49	30.971223239294204	19.059841470690056	19.024297500132427	30.944637789883313
50-54	30.779529548367773	19.110372551207668	19.173595750960814	30.936502149463745
55-59	30.891509256487065	19.211308356375152	19.136584217781614	30.760598169356168
60-64	30.980306333260383	19.037401181791004	18.992236143400724	30.990056341547888
65-69	30.792370000000002	19.126855000000003	19.165875	30.9149
70-74	30.898088538853123	19.21778153066892	19.11868147120888	30.765448459269074
75-79	30.985685256870692	19.042372767784368	19.008884157712203	30.963057817632738
80-84	30.80502197447663	19.132070562983404	19.132980779478398	30.929926683061566
85-89	30.903910991453138	19.224266720040088	19.100511515844	30.771310772662773
90-94	31.004401637854084	19.02973865575244	18.98375826032104	30.982101446072434
95-99	30.822572588428066	19.111030944729084	19.146300707910605	30.92009575893224
100-101	31.007871419194295	19.345735546866063	19.075337940755535	30.571055093184107
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.92694127525857
2	55.958721162479385
3	58.587973658804124
4	60.16289743686698
5	58.768738295217716
6	62.99180874843595
7	61.5007009812736
8	60.26917427213616
9	62.55623651212675
10-14	60.8085036082513
15-19	61.42451802591727
20-24	61.396566253091876
25-29	61.33832451555584
30-34	61.7449859572773
35-39	61.58310080524244
40-44	61.568735126761645
45-49	61.91586102917752
50-54	61.71603169783152
55-59	61.65210742584324
60-64	61.97036267480828
65-69	61.70727
70-74	61.663536998122204
75-79	61.94874307450343
80-84	61.73494865753819
85-89	61.6752217641159
90-94	61.98650308392652
95-99	61.74266834736031
100-101	61.578926512378395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	6.0
2	4.5
3	4.0
4	6.5
5	7.0
6	7.5
7	9.0
8	16.0
9	25.0
10	31.5
11	56.0
12	74.5
13	95.5
14	137.5
15	173.0
16	205.0
17	241.5
18	292.0
19	340.0
20	399.0
21	466.5
22	522.5
23	622.5
24	752.0
25	899.5
26	1053.0
27	1250.5
28	1513.0
29	1750.0
30	2027.0
31	2423.5
32	2833.5
33	3304.0
34	3828.5
35	4326.5
36	4953.0
37	5692.5
38	6504.0
39	7441.5
40	8517.5
41	9896.5
42	11600.0
43	13708.0
44	16476.5
45	19953.0
46	24309.5
47	30176.0
48	37427.0
49	45813.5
50	55638.0
51	67032.5
52	79685.0
53	93454.5
54	108082.5
55	122197.5
56	135603.0
57	148967.0
58	161247.5
59	172132.5
60	181459.0
61	188421.0
62	193799.0
63	197224.0
64	198043.0
65	195870.0
66	190639.0
67	182660.5
68	171619.5
69	157538.5
70	141514.0
71	124619.5
72	106835.0
73	88816.5
74	71601.5
75	56232.0
76	42757.5
77	31434.5
78	22249.0
79	15020.0
80	9908.5
81	6173.5
82	3651.5
83	2148.0
84	1224.5
85	690.0
86	414.5
87	271.5
88	220.5
89	175.5
90	131.0
91	104.0
92	78.0
93	62.5
94	51.5
95	36.0
96	32.0
97	23.5
98	17.0
99	12.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2022
2	0.178875
3	0.175375
4	0.164175
5	0.17435
6	0.15872499999999998
7	0.17565
8	0.16505
9	0.16494999999999999
10-14	0.12474999999999999
15-19	0.08746999999999999
20-24	0.062815
25-29	0.0504
30-34	0.0906
35-39	0.05867
40-44	0.044885
45-49	0.039305
50-54	0.036695
55-59	0.06576
60-64	8.5E-5
65-69	0.0
70-74	5.9999999999999995E-5
75-79	0.01078
80-84	0.023785
85-89	1.65E-4
90-94	8.600000000000001E-4
95-99	0.013505
100-101	0.0037375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2917592985786847
#Duplication Level	Relative count
1	100.0
2	0.19591043235578537
3	0.004008397592957245
4	0.0020041987964786226
5	0.0020041987964786226
6	0.0010020993982393113
7	0.001503149097358967
8	0.0
9	5.010496991196557E-4
10++	0.003006298194717934
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	310060	3.109338	3.7707741	10-14
>>END_MODULE