##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	973637	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48769613315846	34.0	31.0	34.0	31.0	34.0
2	32.7450969920001	34.0	31.0	34.0	31.0	34.0
3	32.80557024846016	34.0	31.0	34.0	31.0	34.0
4	36.284329786152334	37.0	37.0	37.0	35.0	37.0
5	36.18027047041146	37.0	37.0	37.0	35.0	37.0
6	36.1634839267612	37.0	37.0	37.0	35.0	37.0
7	36.143503174180935	37.0	37.0	37.0	35.0	37.0
8	36.11297947797793	37.0	37.0	37.0	35.0	37.0
9	37.882569171056566	39.0	38.0	39.0	35.0	39.0
10-14	38.13169754230786	39.4	38.2	39.4	35.2	39.4
15-19	39.2114812810113	40.4	39.0	41.0	36.0	41.0
20-24	39.01129948841303	40.0	38.6	41.0	35.4	41.0
25-29	38.546837681805435	40.0	38.0	41.0	34.6	41.0
30-34	37.853412308694104	39.8	36.8	41.0	33.0	41.0
35-39	36.86351052805101	38.4	35.0	40.2	31.6	41.0
40-44	35.47753423503832	36.6	34.6	39.6	29.6	41.0
45-49	34.853775072229176	35.0	34.4	38.6	29.2	40.8
50-54	32.947869072354486	35.0	32.4	36.0	23.4	39.6
55-59	33.37790860454153	35.0	33.0	35.0	28.2	37.8
60-64	32.85273772463454	35.0	33.0	35.0	26.8	36.0
65-69	32.82868214745331	35.0	33.0	35.0	29.0	35.0
70-74	32.53225380711702	35.0	33.0	35.0	27.4	35.0
75-79	31.939366314139658	34.4	32.4	35.0	26.4	35.0
80-84	32.14642315359831	35.0	33.0	35.0	27.0	35.0
85-89	31.881814475004546	35.0	33.0	35.0	26.4	35.0
90-94	31.547719324553196	35.0	33.0	35.0	24.8	35.0
95-99	31.01561937354476	34.8	32.0	35.0	23.8	35.0
100-101	29.437635381564178	33.5	29.0	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	2.0
4	7.0
5	21.0
6	76.0
7	208.0
8	538.0
9	796.0
10	1043.0
11	1355.0
12	1425.0
13	1451.0
14	1475.0
15	1691.0
16	1721.0
17	1750.0
18	1891.0
19	2074.0
20	2291.0
21	2613.0
22	3206.0
23	3995.0
24	5198.0
25	6981.0
26	8987.0
27	11260.0
28	14042.0
29	17815.0
30	22061.0
31	28013.0
32	37003.0
33	51678.0
34	86010.0
35	186929.0
36	313830.0
37	137035.0
38	16192.0
39	972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.28912306077123	24.897221645809665	13.907385454269026	22.906269839150077
2	30.03367784913679	24.724512318245917	20.059940203587168	25.181869629030125
3	28.84863660686683	20.215747758148055	22.410508228425993	28.52510740655912
4	30.24412589086076	20.422601030979717	20.82110683961271	28.51216623854681
5	30.90433087485377	21.172572529597787	20.36005205225356	27.563044543294886
6	29.268916444218945	19.522984438759003	17.797495370451205	33.41060374657085
7	32.41957731680287	19.216402006086458	19.64222805830099	28.721792618809676
8	28.344958131213172	19.77759678401704	20.033544329149365	31.843900755620417
9	30.572482352252432	18.252593112217387	19.396962112162953	31.777962423367228
10-14	30.397940916378484	19.662420388707496	19.654532438680945	30.285106256233075
15-19	30.633634506494722	19.22979508790237	19.402076954758293	30.734493450844617
20-24	30.55617522613183	19.253363373319544	19.399105784546087	30.791355616002537
25-29	30.69475842192891	19.396211454377944	19.326286771314034	30.582743352379115
30-34	30.903699611612094	19.101171164838995	19.158346270691037	30.83678295285787
35-39	30.732742343159746	19.229473241278253	19.248951015042675	30.788833400519326
40-44	31.151699248775234	19.266808569472214	19.114661260636804	30.46683092111575
45-49	31.313620990163688	19.228480429564613	18.909490908829472	30.54840767144223
50-54	31.238089760352167	19.308078883608573	19.118110753802494	30.335720602236766
55-59	30.902484702091083	19.22170007532637	19.192941711521154	30.68287351106139
60-64	31.096175311786638	19.114483422296853	18.981823994201363	30.807517271715152
65-69	30.80402119480451	19.0926426285765	19.202552876073835	30.900783300545154
70-74	30.907946185282604	19.20278296736874	19.174969726910543	30.71430112043811
75-79	31.018418568727355	19.076473059261307	19.003612229198357	30.901496142812977
80-84	30.817434488583995	19.132166188966952	19.13294677761707	30.91745254483198
85-89	30.93049217266042	19.215306176533307	19.127502577202044	30.726699073604237
90-94	31.045299882225112	19.01251930815831	19.00171351880259	30.940467290813988
95-99	30.895409347036885	19.088660514682125	19.135310861601713	30.880619276679283
100-101	31.13069860738653	19.293432768064484	19.080673803481176	30.495194821067813
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.19539289992131
2	55.21554747816692
3	57.373744013425956
4	58.75629212940757
5	58.46737541814865
6	62.679520190789795
7	61.14136993561255
8	60.18885888683359
9	62.350444775619664
10-14	60.68304717261156
15-19	61.36812795733933
20-24	61.34753084213437
25-29	61.27750177430802
30-34	61.740482564469964
35-39	61.521575743679065
40-44	61.61853016989098
45-49	61.86202866160592
50-54	61.57381036258893
55-59	61.585358213152475
60-64	61.903692583501794
65-69	61.70480449534966
70-74	61.62224730572071
75-79	61.919914711540336
80-84	61.73488703341597
85-89	61.65719124626465
90-94	61.98576717303911
95-99	61.77602862371616
100-101	61.62589342845434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	1.5
2	0.5
3	0.5
4	0.0
5	1.0
6	2.0
7	1.5
8	3.5
9	4.5
10	4.0
11	5.0
12	4.0
13	5.0
14	12.0
15	20.0
16	26.0
17	29.0
18	43.0
19	60.5
20	73.0
21	85.5
22	92.5
23	123.0
24	161.5
25	187.0
26	236.0
27	281.5
28	327.0
29	420.5
30	488.0
31	554.0
32	685.5
33	831.0
34	929.0
35	1049.0
36	1262.0
37	1437.5
38	1604.5
39	1840.5
40	2057.5
41	2423.5
42	2852.5
43	3282.0
44	3996.0
45	4900.0
46	6006.0
47	7367.0
48	9136.0
49	11364.0
50	13751.0
51	16365.0
52	19520.0
53	23093.0
54	26505.5
55	29777.5
56	33376.5
57	36840.0
58	39592.5
59	42063.5
60	44538.0
61	46314.5
62	47357.0
63	48168.0
64	48285.5
65	47782.5
66	46713.0
67	44520.0
68	41507.5
69	38072.5
70	34273.0
71	29978.0
72	25501.0
73	21336.5
74	17197.0
75	13401.0
76	10160.0
77	7368.0
78	5148.5
79	3500.0
80	2237.5
81	1334.0
82	802.5
83	425.0
84	218.0
85	116.0
86	76.5
87	52.0
88	31.5
89	23.0
90	13.5
91	8.0
92	5.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01879550592263852
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.4379075569231653E-4
25-29	0.0018487382874726411
30-34	0.04993647529828879
35-39	0.0225751486436937
40-44	0.010969180505671005
45-49	0.0
50-54	0.0
55-59	7.394953149890565E-4
60-64	0.017131641463913144
65-69	0.011852466576352378
70-74	0.0
75-79	0.0
80-84	0.0013146583377583226
85-89	0.010332392873319317
90-94	0.008709611487648887
95-99	0.0011092429724835848
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	973637.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.05948988671925596
#Duplication Level	Relative count
1	100.0
2	0.04151764499912463
3	0.0
4	0.0
5	5.002125903508991E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.002125903508991E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	77155	3.1402903	3.7106493	8
AAAAA	78560	3.0620394	3.640423	10-14
>>END_MODULE