##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7358295	34.0	31.0	34.0	31.0	34.0
2	32.975574	34.0	31.0	34.0	31.0	34.0
3	33.02237225	34.0	31.0	34.0	31.0	34.0
4	36.42912675	37.0	37.0	37.0	35.0	37.0
5	36.3573315	37.0	37.0	37.0	35.0	37.0
6	36.33963575	37.0	37.0	37.0	35.0	37.0
7	36.3369735	37.0	37.0	37.0	35.0	37.0
8	36.31431475	37.0	37.0	37.0	35.0	37.0
9	38.116682	39.0	39.0	39.0	37.0	39.0
10-14	38.363531	39.4	38.4	39.4	36.2	39.4
15-19	39.5283408	41.0	40.0	41.0	37.0	41.0
20-24	39.123952149999994	40.8	38.8	41.0	35.6	41.0
25-29	38.709479449999996	40.0	38.0	41.0	35.0	41.0
30-34	37.17329615	39.8	36.2	41.0	31.2	41.0
35-39	36.611259849999996	38.6	35.0	40.4	31.0	41.0
40-44	35.71085065	36.6	35.0	40.0	30.6	41.0
45-49	35.33772295	35.2	35.0	38.8	31.2	41.0
50-54	34.4376807	35.0	34.6	36.6	30.4	39.8
55-59	33.907756750000004	35.0	34.0	35.0	30.4	38.0
60-64	33.3764089	35.0	33.8	35.0	29.8	36.2
65-69	32.977355949999996	35.0	33.0	35.0	29.0	35.0
70-74	32.5240304	35.0	33.0	35.0	27.4	35.0
75-79	32.2336211	34.6	32.6	35.0	27.4	35.0
80-84	32.175606800000004	35.0	33.0	35.0	26.8	35.0
85-89	31.800543800000003	35.0	33.0	35.0	25.8	35.0
90-94	31.6487731	35.0	33.0	35.0	25.0	35.0
95-99	31.27070915	35.0	33.0	35.0	24.2	35.0
100-101	29.915383625	34.0	30.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34.0
3	15.0
4	27.0
5	83.0
6	283.0
7	781.0
8	1923.0
9	3266.0
10	4068.0
11	4541.0
12	4874.0
13	4933.0
14	5251.0
15	5770.0
16	6298.0
17	6936.0
18	7442.0
19	8205.0
20	9084.0
21	10262.0
22	12400.0
23	15064.0
24	18936.0
25	23830.0
26	30383.0
27	38299.0
28	48481.0
29	61526.0
30	78339.0
31	102166.0
32	139925.0
33	211939.0
34	358847.0
35	748688.0
36	1307050.0
37	633982.0
38	80714.0
39	5340.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.45922107273755	24.691939217872623	13.76525678398744	23.08358292540239
2	30.162675	24.713225	19.89165	25.23245
3	28.974725	20.0448	22.1984	28.782075000000003
4	30.322074999999998	20.2835	20.603425	28.791
5	31.01195	21.094025	20.316925	27.577099999999998
6	29.338900000000002	19.529175	17.7416	33.390325
7	32.458725	19.1443	19.61885	28.778125
8	28.3585	19.8248	20.023875	31.792825
9	30.565399999999997	18.225649999999998	19.3992	31.809749999999998
10-14	30.40877	19.651215	19.597990000000003	30.342025
15-19	30.671895	19.179685	19.374485	30.773935
20-24	30.782133595082712	19.214323868252038	19.30053976058652	30.70300277607873
25-29	30.745721680933315	19.36072941551298	19.32282402508746	30.570724878466248
30-34	31.89811762139706	19.005245005388087	18.807146494941602	30.289490878273252
35-39	31.08692797166866	19.132042588633716	19.142074905095587	30.638954534602043
40-44	31.118333502431035	19.228833489404927	19.076995590775436	30.575837417388605
45-49	31.145594999999997	19.07278	18.994510000000002	30.787115
50-54	30.97437	19.15599	19.1173	30.75234
55-59	30.93579964036995	19.20129788019468	19.13882787082418	30.72407460861119
60-64	31.055316271995082	19.027723478506065	18.99096362870431	30.925996620794542
65-69	30.910149172867392	19.125734188679722	19.146587595083655	30.817529043369234
70-74	31.008905	19.244165	19.10405	30.64288
75-79	31.036	19.01611	18.98154	30.96635
80-84	30.924116492100783	19.097353821365793	19.101148876773603	30.877380809759824
85-89	31.01033205150363	19.251269827553095	19.10318829134181	30.63520982960146
90-94	31.081954063397493	18.99987948005395	18.953496803721016	30.964669652827542
95-99	30.90053743682545	19.033711728138947	19.124387879726072	30.941362955309533
100-101	31.106437500000002	19.2385	19.0868	30.5682625
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.54280399813994
2	55.395125
3	57.7568
4	59.113075
5	58.58905
6	62.729225
7	61.23685
8	60.151325
9	62.375150000000005
10-14	60.750795000000004
15-19	61.44583
20-24	61.485136371161445
25-29	61.316446559399566
30-34	62.18760849967031
35-39	61.7258825062707
40-44	61.69417091981963
45-49	61.93271
50-54	61.72671
55-59	61.65987424898114
60-64	61.98131289278962
65-69	61.727678216236626
70-74	61.651785000000004
75-79	62.00234999999999
80-84	61.80149730186061
85-89	61.64554188110509
90-94	62.046623716225035
95-99	61.84190039213499
100-101	61.6747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	3.0
2	2.5
3	2.5
4	3.5
5	7.5
6	7.0
7	7.0
8	13.5
9	16.5
10	17.5
11	23.0
12	32.0
13	38.0
14	59.5
15	82.0
16	97.5
17	135.5
18	168.5
19	200.5
20	251.0
21	327.5
22	419.5
23	517.0
24	614.5
25	757.5
26	935.5
27	1120.0
28	1328.0
29	1604.0
30	2000.5
31	2384.5
32	2780.5
33	3227.5
34	3693.5
35	4222.0
36	4812.0
37	5574.0
38	6396.0
39	7343.5
40	8423.0
41	9793.5
42	11398.0
43	13495.5
44	16397.0
45	19952.0
46	24605.0
47	30223.0
48	37040.0
49	45745.5
50	55736.0
51	66921.0
52	79781.0
53	93715.0
54	108108.5
55	122068.0
56	135658.0
57	148940.5
58	161010.5
59	171675.0
60	181261.0
61	188702.5
62	194231.5
63	197601.5
64	197923.5
65	195543.0
66	190388.0
67	182599.5
68	171836.0
69	158336.5
70	142152.0
71	124814.5
72	107219.0
73	89464.0
74	72385.0
75	56569.0
76	42941.5
77	31541.5
78	22252.5
79	15167.5
80	9952.0
81	6158.0
82	3678.5
83	2180.5
84	1211.5
85	668.5
86	390.0
87	264.5
88	202.0
89	141.5
90	99.5
91	64.5
92	49.5
93	40.5
94	25.0
95	13.5
96	8.0
97	7.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0034999999999999996
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001035
25-29	0.0010299999999999999
30-34	0.057300000000000004
35-39	0.02309
40-44	0.008495
45-49	0.0
50-54	0.0
55-59	1.4999999999999999E-5
60-64	0.026795
65-69	0.016335000000000002
70-74	0.0
75-79	0.0
80-84	0.0014600000000000001
85-89	0.01792
90-94	0.016550000000000002
95-99	0.00127
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.12949093563450734
#Duplication Level	Relative count
1	100.0
2	0.11964656704462967
3	0.0025030662561638006
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006132512327601E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	313265	3.139192	3.6462948	10-14
AAAAA	316345	3.0411503	3.547551	1
>>END_MODULE