##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.662347	34.0	31.0	34.0	31.0	34.0
2	32.9087895	34.0	31.0	34.0	31.0	34.0
3	32.97548875	34.0	31.0	34.0	31.0	34.0
4	36.405341	37.0	37.0	37.0	35.0	37.0
5	36.3283065	37.0	37.0	37.0	35.0	37.0
6	36.30182675	37.0	37.0	37.0	35.0	37.0
7	36.2862565	37.0	37.0	37.0	35.0	37.0
8	36.2847795	37.0	37.0	37.0	35.0	37.0
9	38.04945225	39.0	38.0	39.0	35.0	39.0
10-14	38.3239413	39.4	38.4	39.4	35.4	39.4
15-19	39.4344421	41.0	39.0	41.0	36.6	41.0
20-24	38.7267356	40.4	38.8	41.0	34.6	41.0
25-29	29.014838250000004	29.2	25.6	31.4	22.0	39.4
30-34	33.5356635	37.4	31.6	39.4	19.0	40.2
35-39	35.41585210000001	37.6	34.0	40.0	27.6	41.0
40-44	34.03021095	35.8	33.6	39.4	19.8	41.0
45-49	34.61779105	35.0	34.2	38.4	29.0	40.8
50-54	33.1839644	35.0	33.2	36.0	24.8	39.4
55-59	33.4440433	35.0	33.2	35.0	29.2	37.6
60-64	31.92373725	35.0	32.4	35.0	23.2	35.4
65-69	32.50062095	35.0	33.0	35.0	27.8	35.0
70-74	31.230729349999997	35.0	31.4	35.0	21.2	35.0
75-79	31.656206450000003	34.4	32.4	35.0	25.6	35.0
80-84	31.5016331	35.0	32.4	35.0	24.0	35.0
85-89	30.93666025	34.6	31.6	35.0	20.8	35.0
90-94	30.976626349999997	34.6	31.6	35.0	23.6	35.0
95-99	30.548236250000002	34.4	31.6	35.0	20.8	35.0
100-101	28.9788435	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34.0
3	19.0
4	40.0
5	135.0
6	502.0
7	1775.0
8	5133.0
9	6006.0
10	5338.0
11	6098.0
12	6142.0
13	6197.0
14	6749.0
15	7340.0
16	8160.0
17	8756.0
18	9682.0
19	10489.0
20	11728.0
21	13638.0
22	16757.0
23	21659.0
24	27484.0
25	35384.0
26	45085.0
27	56731.0
28	71931.0
29	93134.0
30	124173.0
31	174924.0
32	275514.0
33	470428.0
34	732079.0
35	917679.0
36	637363.0
37	166654.0
38	18099.0
39	957.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.39998639784406	24.794579940920634	13.77368312877591	23.031750532459395
2	30.106875	24.717624999999998	19.96825	25.20725
3	28.92905	20.095575	22.242875	28.732499999999998
4	30.264999999999997	20.3261	20.673175	28.735725000000002
5	31.018875	21.103675	20.29655	27.580900000000003
6	29.32175	19.532075	17.779425	33.36675
7	32.4341	19.2221	19.60935	28.73445
8	28.373575000000002	19.89685	20.0175	31.712075000000002
9	30.51085	18.235325	19.394275	31.85955
10-14	30.429675	19.64735	19.601275	30.3217
15-19	30.665695	19.196495	19.381155	30.756655
20-24	30.931315349823823	19.208083774175968	19.28251464129557	30.57808623470463
25-29	39.16323362312548	17.277687972421266	16.870875126589702	26.688203277863554
30-34	32.3296715350529	19.12961332739247	18.62064158132679	29.920073556227837
35-39	31.15731987018767	19.15112496382403	19.14940932509173	30.542145840896566
40-44	31.060373659029754	19.099802396768904	19.2065694138792	30.63325453032214
45-49	31.046215	19.131855	18.994985	30.826945
50-54	31.05983460694023	19.205825307768194	19.123698788427586	30.610641296863996
55-59	30.896875728917	19.213488398953885	19.143677369241196	30.745958502887916
60-64	31.191011186497413	19.032841810115556	18.904401320158954	30.871745683228074
65-69	30.88271816475799	19.124887460012946	19.158331200373674	30.83406317485539
70-74	31.109735	19.321749999999998	19.06831	30.500205
75-79	31.20116	19.0338	18.93959	30.82545
80-84	30.923438984908046	19.072880855741055	19.17329520368232	30.83038495566858
85-89	31.110866832851457	19.340601734037786	19.086198373906974	30.462333059203782
90-94	31.085193070685634	18.990374718281053	18.972500188475486	30.951932022557827
95-99	30.937026640771155	19.049772927574733	19.106764791208672	30.90643564044544
100-101	31.220237499999996	19.245075	19.064625	30.4700625
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.43173693030345
2	55.314125000000004
3	57.66154999999999
4	59.000724999999996
5	58.599775
6	62.688500000000005
7	61.168549999999996
8	60.08565
9	62.370400000000004
10-14	60.751374999999996
15-19	61.42235
20-24	61.509401584528455
25-29	65.85143690098903
30-34	62.24974509128074
35-39	61.69946571108424
40-44	61.69362818935189
45-49	61.873160000000006
50-54	61.670475903804224
55-59	61.64283423180492
60-64	62.06275686972549
65-69	61.71678133961338
70-74	61.609939999999995
75-79	62.026610000000005
80-84	61.75382394057662
85-89	61.57319989205524
90-94	62.03712509324346
95-99	61.8434622812166
100-101	61.6903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	2.0
3	2.5
4	5.0
5	4.5
6	4.5
7	4.5
8	4.0
9	8.0
10	17.5
11	22.5
12	25.5
13	33.0
14	50.0
15	65.0
16	86.5
17	109.0
18	126.5
19	174.5
20	211.0
21	275.0
22	351.5
23	414.0
24	504.5
25	635.0
26	814.5
27	999.5
28	1238.5
29	1484.5
30	1754.0
31	2094.5
32	2437.5
33	2924.0
34	3442.0
35	4027.5
36	4662.5
37	5240.5
38	5974.0
39	6993.5
40	8115.0
41	9340.0
42	10916.5
43	12848.0
44	15549.0
45	18837.5
46	23059.0
47	28649.0
48	35381.0
49	43571.0
50	53235.5
51	64421.5
52	77055.0
53	90793.0
54	105479.0
55	120195.5
56	134786.5
57	148633.0
58	161016.0
59	172696.0
60	182521.5
61	190209.5
62	196249.0
63	200012.0
64	200900.5
65	198956.0
66	194122.0
67	185721.5
68	174279.0
69	160354.0
70	143807.0
71	126199.0
72	108297.5
73	90008.0
74	72412.0
75	56503.0
76	42906.5
77	31524.5
78	22408.5
79	15300.5
80	9879.0
81	6157.0
82	3742.5
83	2185.0
84	1287.5
85	783.5
86	478.5
87	309.5
88	227.5
89	155.5
90	102.5
91	77.5
92	46.0
93	28.0
94	19.5
95	14.0
96	9.0
97	4.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01585
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001165
25-29	0.046175
30-34	0.08188
35-39	0.03723
40-44	2.0765
45-49	0.0
50-54	0.0018499999999999999
55-59	0.001475
60-64	0.039310000000000005
65-69	0.041084999999999997
70-74	0.0
75-79	0.0
80-84	0.0043300000000000005
85-89	0.02294
90-94	0.053315
95-99	0.0032700000000000003
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.0339993200135883
#Duplication Level	Relative count
1	100.0
2	0.031009923175416135
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010003201024327785
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	291200	3.043503	3.6781611	10-14
>>END_MODULE