##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79486925	34.0	31.0	34.0	31.0	34.0
2	33.02010575	34.0	31.0	34.0	31.0	34.0
3	33.06467625	34.0	31.0	34.0	31.0	34.0
4	36.4548635	37.0	37.0	37.0	35.0	37.0
5	36.388087	37.0	37.0	37.0	35.0	37.0
6	36.36473625	37.0	37.0	37.0	35.0	37.0
7	36.36214275	37.0	37.0	37.0	35.0	37.0
8	36.34141	37.0	37.0	37.0	35.0	37.0
9	38.131392	39.0	39.0	39.0	37.0	39.0
10-14	38.3949124	39.4	38.4	39.4	37.0	39.4
15-19	39.5385054	41.0	39.8	41.0	37.0	41.0
20-24	39.31300875	40.8	39.0	41.0	36.0	41.0
25-29	38.92487995	40.0	38.4	41.0	35.0	41.0
30-34	38.2447362	40.0	37.4	41.0	34.0	41.0
35-39	37.2981821	38.8	35.2	40.6	33.0	41.0
40-44	36.34572315	37.2	35.0	40.0	32.2	41.0
45-49	35.75846035	35.2	35.0	39.2	32.8	41.0
50-54	34.86195305	35.0	35.0	36.6	31.6	40.0
55-59	34.18093760000001	35.0	34.4	35.2	31.0	38.0
60-64	33.659187200000005	35.0	34.0	35.0	30.6	36.4
65-69	33.28064755	35.0	33.4	35.0	30.0	35.0
70-74	33.02126360000001	35.0	33.0	35.0	29.2	35.0
75-79	32.41765025	34.4	32.6	35.0	28.2	35.0
80-84	32.7228697	35.0	33.0	35.0	29.0	35.0
85-89	32.472879750000004	35.0	33.0	35.0	28.6	35.0
90-94	32.0838679	35.0	33.0	35.0	27.0	35.0
95-99	31.608108549999997	35.0	33.0	35.0	26.2	35.0
100-101	30.3458275	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33.0
3	8.0
4	26.0
5	72.0
6	201.0
7	637.0
8	1503.0
9	2351.0
10	3094.0
11	3447.0
12	3633.0
13	3774.0
14	4148.0
15	4463.0
16	5106.0
17	5693.0
18	6250.0
19	6725.0
20	7590.0
21	8455.0
22	10087.0
23	12571.0
24	16185.0
25	20537.0
26	26583.0
27	33958.0
28	42734.0
29	53721.0
30	68625.0
31	88366.0
32	116681.0
33	164164.0
34	267567.0
35	663282.0
36	1467965.0
37	772993.0
38	99677.0
39	7075.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40280283193434	24.758823792668842	13.768089309390351	23.070284066006465
2	30.125649999999997	24.657825	19.921025	25.295499999999997
3	28.95615	20.0874	22.182	28.77445
4	30.299125	20.3253	20.58125	28.794324999999997
5	31.030724999999997	21.0676	20.275125	27.626549999999998
6	29.354599999999998	19.543125	17.728250000000003	33.374025
7	32.4446	19.217775	19.618475	28.71915
8	28.358800000000002	19.793025	20.0066	31.841575
9	30.512099999999997	18.2197	19.372075	31.896124999999998
10-14	30.443595000000002	19.637999999999998	19.585255	30.333149999999996
15-19	30.673485	19.17041	19.37418	30.781924999999998
20-24	30.603876407177005	19.208028318417696	19.379900853537592	30.808194420867707
25-29	30.71672075085226	19.349406772292372	19.332546766391367	30.601325710464
30-34	30.88933550728447	19.14443262461186	19.12660201064305	30.839629857460622
35-39	30.74890507963346	19.172348258605467	19.249687345976234	30.82905931578484
40-44	30.860239865659633	19.255192951780618	19.175737733278766	30.708829449280984
45-49	30.963089999999998	19.04538	19.05148	30.940050000000003
50-54	30.806955000000002	19.102520000000002	19.182859999999998	30.907665
55-59	30.880904704723765	19.199770359816288	19.165935332748266	30.753389602711685
60-64	31.017200472270844	19.007150836815217	19.01177751051443	30.96387118039951
65-69	30.814928566852522	19.08918192171013	19.15773765867955	30.9381518527578
70-74	30.920920000000002	19.194045000000003	19.12971	30.755325
75-79	31.031924999999998	19.009685	19.007545	30.950844999999997
80-84	30.843741626293287	19.071603152041337	19.14907064262116	30.935584579044217
85-89	30.915020163251544	19.18954086622535	19.128129110027672	30.767309860495434
90-94	31.048759025137596	18.986896889137764	18.984356111405702	30.97998797431894
95-99	30.880454141473784	19.047841204894198	19.126276295331568	30.945428358300454
100-101	31.099147050238845	19.27448957078758	19.08039928073966	30.545964098233913
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.47308689794081
2	55.42115
3	57.730599999999995
4	59.093450000000004
5	58.657275
6	62.72862500000001
7	61.16374999999999
8	60.200374999999994
9	62.408224999999995
10-14	60.776745
15-19	61.45541
20-24	61.41207082804472
25-29	61.318046461316264
30-34	61.72896536474509
35-39	61.57796439541829
40-44	61.56906931494062
45-49	61.90314
50-54	61.71462
55-59	61.63429430743545
60-64	61.98107165267035
65-69	61.75308041961032
70-74	61.676244999999994
75-79	61.982769999999995
80-84	61.779326205337505
85-89	61.68233002374698
90-94	62.02874699945653
95-99	61.82588249977423
100-101	61.645111148472765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	5.0
2	1.5
3	3.0
4	3.0
5	2.5
6	5.0
7	6.5
8	8.5
9	14.0
10	19.0
11	25.0
12	31.0
13	41.5
14	53.5
15	80.0
16	104.0
17	128.5
18	157.0
19	205.0
20	275.5
21	347.0
22	414.5
23	498.5
24	611.5
25	759.5
26	968.0
27	1170.5
28	1419.5
29	1683.5
30	1946.0
31	2323.5
32	2817.0
33	3289.5
34	3784.5
35	4331.5
36	4870.0
37	5575.0
38	6401.5
39	7325.0
40	8429.5
41	9832.5
42	11483.0
43	13627.0
44	16451.5
45	20005.5
46	24601.5
47	30316.5
48	37463.5
49	46166.0
50	56324.5
51	67829.0
52	80661.0
53	94113.5
54	108225.0
55	122665.5
56	136323.5
57	149541.0
58	161472.5
59	172124.5
60	181338.0
61	188141.0
62	193772.5
63	197421.0
64	197758.0
65	195538.5
66	190919.5
67	182946.0
68	171326.0
69	157518.0
70	141894.0
71	124337.5
72	106282.5
73	88669.5
74	71833.5
75	56303.5
76	42754.5
77	31274.5
78	22085.5
79	15000.0
80	9548.0
81	5855.5
82	3477.0
83	1987.5
84	1097.5
85	606.5
86	349.5
87	204.5
88	127.5
89	97.0
90	65.5
91	39.0
92	24.0
93	15.0
94	11.0
95	8.0
96	5.0
97	1.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008275000000000001
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001475
25-29	3.5E-5
30-34	0.031485
35-39	0.005285
40-44	2.75E-4
45-49	0.0
50-54	0.0
55-59	7.999999999999999E-5
60-64	0.036175
65-69	0.022955
70-74	0.0
75-79	0.0
80-84	0.0032150000000000004
85-89	0.027285
90-94	0.030610000000000002
95-99	0.006490000000000001
100-101	0.0014375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15898569128777598
#Duplication Level	Relative count
1	100.0
2	0.14621932899349024
3	0.0025037556334501754
4	5.00751126690035E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.00100150225338007
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE