##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8139035	34.0	31.0	34.0	31.0	34.0
2	33.04723675	34.0	31.0	34.0	31.0	34.0
3	33.08596825	34.0	33.0	34.0	31.0	34.0
4	36.47270775	37.0	37.0	37.0	35.0	37.0
5	36.41430975	37.0	37.0	37.0	35.0	37.0
6	36.39158375	37.0	37.0	37.0	35.0	37.0
7	36.38054225	37.0	37.0	37.0	35.0	37.0
8	36.36056375	37.0	37.0	37.0	35.0	37.0
9	38.15680325	39.0	39.0	39.0	37.0	39.0
10-14	38.41936439999999	39.4	38.6	39.4	37.0	39.4
15-19	39.566529200000005	41.0	40.0	41.0	37.0	41.0
20-24	39.3248352	40.6	39.0	41.0	36.2	41.0
25-29	38.90269205	40.0	38.4	41.0	35.0	41.0
30-34	38.18734205	40.0	37.4	41.0	34.0	41.0
35-39	37.20264185	38.8	35.2	40.6	32.8	41.0
40-44	36.292679449999994	37.2	35.0	40.0	32.0	41.0
45-49	35.70665295	35.2	35.0	39.2	32.4	41.0
50-54	34.7770913	35.0	35.0	36.6	31.4	40.0
55-59	34.063191849999995	35.0	34.0	35.0	31.0	38.0
60-64	33.5203063	35.0	33.8	35.0	30.2	36.4
65-69	33.10713415	35.0	33.0	35.0	29.4	35.0
70-74	32.81857475	35.0	33.0	35.0	29.0	35.0
75-79	32.20260555	34.4	32.6	35.0	27.8	35.0
80-84	32.42383625	35.0	33.0	35.0	27.8	35.0
85-89	32.150539450000004	35.0	33.0	35.0	27.0	35.0
90-94	31.738831549999997	35.0	33.0	35.0	26.2	35.0
95-99	31.1716931	35.0	32.6	35.0	24.0	35.0
100-101	29.803890250000002	34.0	30.5	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17.0
3	6.0
4	27.0
5	78.0
6	231.0
7	730.0
8	1730.0
9	2584.0
10	3485.0
11	3932.0
12	4264.0
13	4350.0
14	4706.0
15	5341.0
16	5927.0
17	6747.0
18	7263.0
19	8087.0
20	8474.0
21	9774.0
22	11451.0
23	13863.0
24	17620.0
25	22385.0
26	28750.0
27	35693.0
28	44786.0
29	55933.0
30	71019.0
31	91664.0
32	122062.0
33	172309.0
34	284137.0
35	687744.0
36	1430930.0
37	731383.0
38	94139.0
39	6351.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.403924935410274	24.725753038479827	13.772750159880514	23.097571866229387
2	30.154799999999998	24.64115	19.908275	25.295775
3	28.972949999999997	20.065825	22.179750000000002	28.781475
4	30.32395	20.2431	20.585125	28.847825
5	31.006275	21.12015	20.287225	27.58635
6	29.35915	19.527275	17.7654	33.348175000000005
7	32.470775	19.14455	19.6424	28.742275
8	28.328599999999998	19.858575000000002	20.031025	31.7818
9	30.526375	18.20675	19.3967	31.870175
10-14	30.43951	19.64247	19.595945	30.322074999999998
15-19	30.67804	19.17481	19.376185	30.770965
20-24	30.59247574341033	19.21962509368208	19.37605647809983	30.81184268480776
25-29	30.733696557074534	19.34479425138079	19.33795418092806	30.58355501061661
30-34	30.920336443331646	19.113302286713242	19.123738097372616	30.842623172582496
35-39	30.7417677566715	19.167330998844555	19.25474181156209	30.83615943292185
40-44	30.864157159902394	19.24994201165047	19.192321167505106	30.69357966094203
45-49	30.98352	19.045985	19.040935	30.929560000000002
50-54	30.80222433280891	19.108646852541074	19.1825429425084	30.906585872141612
55-59	30.88986319293225	19.19468965796208	19.14587445537899	30.769572693726676
60-64	31.02065616586674	19.0010387027954	19.00472495483091	30.97358017650695
65-69	30.833954149389477	19.068103208762498	19.174936086580647	30.923006555267385
70-74	30.924970000000002	19.19475	19.127635	30.752645
75-79	31.041555	18.985979999999998	18.99573	30.976734999999998
80-84	30.86237597913969	19.076597099459853	19.135406360190796	30.92562056120966
85-89	30.941387348575745	19.1814534478017	19.127210953043033	30.74994825057952
90-94	31.065438813142066	18.98906368548937	18.94875204484411	30.996745456524454
95-99	30.907263398614077	19.03388437260934	19.11415582242033	30.944696406356247
100-101	31.14826066320438	19.240112116163203	19.016047291490647	30.595579929141763
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.501496801639654
2	55.450575
3	57.754425
4	59.171775000000004
5	58.592625
6	62.707325000000004
7	61.21305
8	60.1104
9	62.39655
10-14	60.761585
15-19	61.449005
20-24	61.40431842821808
25-29	61.317251567691144
30-34	61.762959615914134
35-39	61.57792718959335
40-44	61.557736820844426
45-49	61.91308
50-54	61.708810204950524
55-59	61.65943588665893
60-64	61.99423634237369
65-69	61.75696070465686
70-74	61.677614999999996
75-79	62.01829
80-84	61.78799654034934
85-89	61.69133559915526
90-94	62.06218426966652
95-99	61.85195980497033
100-101	61.743840592346146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.0
2	4.0
3	3.0
4	2.5
5	2.5
6	4.0
7	6.0
8	6.5
9	11.0
10	17.0
11	22.5
12	32.5
13	43.0
14	54.0
15	69.5
16	94.0
17	122.5
18	160.5
19	206.5
20	255.0
21	319.5
22	429.0
23	506.5
24	594.5
25	751.5
26	935.5
27	1144.5
28	1372.0
29	1637.5
30	1938.5
31	2275.5
32	2741.0
33	3255.5
34	3743.0
35	4333.0
36	4966.0
37	5674.5
38	6507.5
39	7376.5
40	8409.5
41	9740.0
42	11404.5
43	13615.0
44	16344.0
45	19921.0
46	24576.5
47	30328.0
48	37467.5
49	46082.5
50	56266.0
51	67666.5
52	80269.5
53	94116.0
54	108231.5
55	122672.5
56	136880.5
57	149758.0
58	161785.0
59	172614.0
60	181672.0
61	189116.0
62	194170.0
63	197188.5
64	197983.5
65	195556.0
66	190191.0
67	181904.5
68	170902.0
69	157257.0
70	141332.5
71	123826.0
72	106023.0
73	88631.0
74	71619.0
75	56084.5
76	42683.5
77	31304.0
78	22097.0
79	15068.5
80	9960.0
81	6289.5
82	3783.0
83	2210.0
84	1260.5
85	728.5
86	441.5
87	291.5
88	211.0
89	148.0
90	94.0
91	63.0
92	48.0
93	34.0
94	24.0
95	13.5
96	9.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00345
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	8.85E-4
25-29	0.0010299999999999999
30-34	0.05568
35-39	0.012369999999999999
40-44	0.001465
45-49	0.0
50-54	0.001475
55-59	4.15E-4
60-64	0.033965
65-69	0.030775000000000004
70-74	0.0
75-79	0.0
80-84	0.007245
85-89	0.023035
90-94	0.04128
95-99	0.008035
100-101	0.0010375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15848098228212912
#Duplication Level	Relative count
1	100.0
2	0.1442112695098319
3	0.001502200724060749
4	0.0
5	5.007335746869164E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007335746869164E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	302535	3.0048015	3.638898	10-14
>>END_MODULE