##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.827921	34.0	31.0	34.0	31.0	34.0
2	33.054472	34.0	31.0	34.0	31.0	34.0
3	33.10746825	34.0	33.0	34.0	31.0	34.0
4	36.48093675	37.0	37.0	37.0	35.0	37.0
5	36.41943125	37.0	37.0	37.0	35.0	37.0
6	36.389364	37.0	37.0	37.0	35.0	37.0
7	36.38413075	37.0	37.0	37.0	35.0	37.0
8	36.35954525	37.0	37.0	37.0	35.0	37.0
9	38.152515	39.0	39.0	39.0	37.0	39.0
10-14	38.429414	39.4	38.8	39.4	37.0	39.4
15-19	39.564372649999996	41.0	39.8	41.0	37.0	41.0
20-24	39.3298053	40.8	39.0	41.0	36.2	41.0
25-29	38.88607095	40.0	38.4	41.0	35.0	41.0
30-34	38.17225545	40.0	37.4	41.0	33.8	41.0
35-39	37.1955015	38.8	35.2	40.6	32.8	41.0
40-44	36.2472354	37.2	35.0	40.0	31.8	41.0
45-49	35.65820975	35.2	35.0	39.2	32.0	41.0
50-54	34.72647825	35.0	35.0	36.6	31.2	40.0
55-59	33.99032905	35.0	34.0	35.2	30.8	38.0
60-64	33.410817	35.0	33.8	35.0	30.0	36.2
65-69	32.9924372	35.0	33.0	35.0	29.0	35.0
70-74	32.6864482	35.0	33.0	35.0	29.0	35.0
75-79	32.070710150000004	34.4	32.6	35.0	26.8	35.0
80-84	32.28281725	35.0	33.0	35.0	27.0	35.0
85-89	31.973857399999996	35.0	33.0	35.0	27.0	35.0
90-94	31.4840512	35.0	33.0	35.0	24.4	35.0
95-99	30.869056049999994	35.0	32.0	35.0	22.6	35.0
100-101	29.523152375	34.0	30.5	35.0	10.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	8.0
4	25.0
5	73.0
6	273.0
7	765.0
8	1856.0
9	3149.0
10	4066.0
11	4676.0
12	4936.0
13	5044.0
14	5503.0
15	5995.0
16	6692.0
17	7329.0
18	8137.0
19	8903.0
20	9647.0
21	10689.0
22	12761.0
23	15308.0
24	18669.0
25	23631.0
26	29777.0
27	36879.0
28	46196.0
29	57973.0
30	73060.0
31	93810.0
32	125379.0
33	175816.0
34	287137.0
35	673331.0
36	1398094.0
37	739129.0
38	98278.0
39	6947.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.44522886916326	24.689426318166067	13.733174914877583	23.13216989779309
2	30.138949999999998	24.676125	19.8986	25.286324999999998
3	28.93825	20.03265	22.225524999999998	28.803575
4	30.313950000000002	20.228575	20.571025000000002	28.886450000000004
5	30.95725	21.161350000000002	20.318749999999998	27.562649999999998
6	29.3171	19.529374999999998	17.8141	33.339425
7	32.459900000000005	19.18005	19.599275	28.760775
8	28.3371	19.8761	20.014625	31.772175000000004
9	30.503400000000003	18.23705	19.380875	31.878675
10-14	30.412055	19.659979999999997	19.607115	30.32085
15-19	30.675960000000003	19.19887	19.378845	30.746325000000002
20-24	30.58479808190005	19.232960022784237	19.399131750970213	30.7831101443455
25-29	30.729271589417774	19.37422677479357	19.32676637849926	30.5697352572894
30-34	30.889897126901904	19.13240698714146	19.116516931914514	30.861178954042117
35-39	30.729172214513373	19.184105096007567	19.266389508122348	30.820333181356713
40-44	30.867596867968278	19.26013605711571	19.1931895240401	30.679077550875917
45-49	30.979855	19.030645	19.051895	30.937605
50-54	30.784778984532963	19.093019927877357	19.192995817662776	30.9292052699269
55-59	30.90116	19.19119	19.158810000000003	30.74884
60-64	31.0106439218094	19.025001197840066	18.997088273360806	30.967266606989725
65-69	30.826267239086697	19.088445457265042	19.169958942152334	30.915328361495924
70-74	30.954135	19.180315	19.10238	30.763170000000002
75-79	31.04988	19.00905	18.968964999999997	30.972105
80-84	30.86394652083233	19.067529870287935	19.12952039674337	30.939003212136363
85-89	30.96930383655784	19.177198193066623	19.094162873050077	30.759335097325458
90-94	31.10943944631901	18.97040511353345	18.953332189135587	30.966823251011956
95-99	30.958669129818727	19.010273112252367	19.093570509061205	30.9374872488677
100-101	31.209600554003114	19.211308063607856	19.003419394234093	30.575671988154934
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.57739876695635
2	55.425275
3	57.741825000000006
4	59.200399999999995
5	58.5199
6	62.656524999999995
7	61.220675
8	60.109275
9	62.382075
10-14	60.732905
15-19	61.422285
20-24	61.36790822624555
25-29	61.29900684670717
30-34	61.751076080944024
35-39	61.549505395870085
40-44	61.54667441884419
45-49	61.917460000000005
50-54	61.71398425445987
55-59	61.650000000000006
60-64	61.97791052879913
65-69	61.74159560058262
70-74	61.717305
75-79	62.021985
80-84	61.80294973296869
85-89	61.7286389338833
90-94	62.07626269733096
95-99	61.89615637868643
100-101	61.785272542158054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	7.5
2	4.0
3	2.5
4	1.5
5	2.0
6	4.0
7	7.0
8	11.0
9	13.5
10	16.0
11	26.0
12	36.0
13	45.5
14	58.5
15	70.0
16	98.5
17	120.5
18	146.0
19	206.0
20	271.0
21	328.0
22	401.0
23	501.0
24	640.0
25	796.5
26	969.5
27	1167.5
28	1392.5
29	1679.0
30	1966.0
31	2303.5
32	2770.0
33	3260.0
34	3780.5
35	4347.0
36	4992.5
37	5737.0
38	6551.5
39	7358.0
40	8455.5
41	9878.0
42	11423.5
43	13576.5
44	16561.0
45	20237.5
46	24623.5
47	30333.0
48	37609.0
49	46227.5
50	56272.5
51	67899.0
52	80900.5
53	94326.0
54	108337.5
55	122872.0
56	136784.5
57	149736.0
58	161680.5
59	172282.5
60	181275.5
61	188738.5
62	193697.0
63	196433.0
64	197445.0
65	195317.0
66	190101.5
67	181455.5
68	170325.0
69	157014.0
70	141156.5
71	123977.0
72	106012.5
73	88185.0
74	71513.5
75	56206.0
76	42633.0
77	31475.0
78	22414.0
79	15374.5
80	10152.5
81	6407.5
82	4001.0
83	2449.0
84	1441.0
85	880.5
86	566.0
87	376.0
88	250.0
89	196.0
90	136.0
91	92.0
92	67.5
93	54.5
94	36.0
95	16.5
96	10.5
97	5.0
98	2.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01165
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0010400000000000001
25-29	8.35E-4
30-34	0.06328
35-39	0.017515
40-44	0.00229
45-49	0.0
50-54	8.9E-4
55-59	0.0
60-64	0.028390000000000002
65-69	0.034945000000000004
70-74	0.0
75-79	0.0
80-84	0.008915
85-89	0.01845
90-94	0.046415
95-99	0.00888
100-101	5.625000000000001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1619870410367148
#Duplication Level	Relative count
1	100.0
2	0.15273521222682931
3	0.0010015423752578973
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007711876289486E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	308685	3.0679076	3.5718718	10-14
>>END_MODULE