##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	767247	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17296646321198	33.0	31.0	34.0	30.0	34.0
2	32.33964942189412	34.0	31.0	34.0	30.0	34.0
3	32.41200030759325	34.0	31.0	34.0	30.0	34.0
4	35.85267586579029	37.0	35.0	37.0	35.0	37.0
5	35.87438074049165	37.0	35.0	37.0	35.0	37.0
6	35.85349176992546	37.0	36.0	37.0	35.0	37.0
7	35.81887058535256	37.0	36.0	37.0	35.0	37.0
8	35.828501121542345	37.0	36.0	37.0	35.0	37.0
9	37.52813370400927	39.0	38.0	39.0	35.0	39.0
10-14	37.70281278388837	39.4	38.0	39.4	35.0	39.4
15-19	38.79184617209321	40.4	38.2	41.0	35.0	41.0
20-24	38.573624921309566	40.0	38.0	41.0	34.4	41.0
25-29	38.157477578928294	40.0	38.0	41.0	33.2	41.0
30-34	37.50109964587675	39.8	36.6	41.0	32.4	41.0
35-39	36.52159083059301	38.4	35.0	40.4	31.0	41.0
40-44	35.609483777714345	36.6	35.0	40.0	30.0	41.0
45-49	34.71396825272696	35.0	34.2	38.4	29.6	40.8
50-54	33.39112541332843	34.8	32.8	36.2	27.2	39.2
55-59	33.443553640483444	35.0	34.0	35.0	28.6	38.0
60-64	33.02065280150981	35.0	33.4	35.0	27.4	36.2
65-69	32.69033648877089	35.0	33.0	35.0	27.8	35.0
70-74	32.36155631758743	35.0	33.0	35.0	26.0	35.0
75-79	32.16075005832541	35.0	33.0	35.0	27.0	35.0
80-84	31.901925194885088	35.0	33.0	35.0	25.8	35.0
85-89	31.60008941058095	35.0	33.0	35.0	24.4	35.0
90-94	31.17183045355668	35.0	32.4	35.0	23.8	35.0
95-99	30.59532093315451	35.0	31.6	35.0	20.6	35.0
100-101	29.289823876795868	34.0	29.5	35.0	8.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1035.0
3	278.0
4	377.0
5	438.0
6	690.0
7	862.0
8	1085.0
9	1322.0
10	1319.0
11	1436.0
12	1370.0
13	1319.0
14	1348.0
15	1418.0
16	1490.0
17	1571.0
18	1860.0
19	2036.0
20	2376.0
21	2862.0
22	3430.0
23	4291.0
24	5129.0
25	6129.0
26	7511.0
27	8891.0
28	10957.0
29	13538.0
30	16043.0
31	20110.0
32	26132.0
33	35375.0
34	55400.0
35	125006.0
36	252944.0
37	130819.0
38	17532.0
39	1506.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.33230569485524	24.455145209879408	13.552835174174946	23.6597139210904
2	30.528747138842398	24.873822297998363	19.048140443081866	25.549290120077373
3	29.33288666190449	19.929453722580543	21.446224757415703	29.291434858099265
4	30.628038845075928	19.92765430489474	19.949814247539592	29.494492602489736
5	31.161616359112127	21.098150240947053	20.119763132820026	27.620470267120794
6	29.204439549267136	19.474449129621544	17.583143790040342	33.73796753107098
7	32.489252932144225	19.027980176906254	19.475068627425422	29.0076982635241
8	28.31920121486251	19.86691259377057	19.826504076697972	31.987382114668943
9	30.61471899845007	18.190634976294792	19.255120702982932	31.939525322272207
10-14	30.468415589209492	19.66310031339627	19.520479202760765	30.348004894633473
15-19	30.65916173234266	19.181429667048484	19.343705797676467	30.81570280293239
20-24	30.581494616466404	19.249941674584587	19.319984307530692	30.84857940141832
25-29	30.727106134009	19.39920260359441	19.29957367053895	30.574117591857643
30-34	30.90239056391199	19.178679963275833	19.02704418943289	30.89188528337929
35-39	30.7463155002625	19.216694828171377	19.222247189019566	30.81474248254656
40-44	30.864428274076015	19.303848695400568	19.14066786836573	30.69105516215769
45-49	31.01037866554056	19.101593098441572	18.987809662338204	30.90021857367966
50-54	30.77213626826567	19.164376873422768	19.186325522446925	30.87716133586464
55-59	30.858910066487084	19.29629423022683	19.117186282171524	30.72760942111456
60-64	30.96838436644262	19.068826596910775	19.005613576853346	30.957175459793262
65-69	30.74514853833792	19.15369706859496	19.196994954662344	30.904159438404783
70-74	30.901626203817024	19.275383285956153	19.107223618991014	30.71576689123581
75-79	31.017833891823628	19.06595920218652	18.983664973600415	30.93254193238944
80-84	30.79613214518923	19.15083408602445	19.155943262078576	30.897090506707748
85-89	30.93530505821463	19.254373102794798	19.096614258511273	30.713707580479298
90-94	31.04012136899851	19.07239780670371	18.971726184657616	30.91575463964017
95-99	30.859475501370486	19.135610826761134	19.09773514917621	30.90717852269217
100-101	31.0774105340262	19.359932329484508	19.125327306590968	30.437329829898324
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.99201961594565
2	56.07803725891978
3	58.624321520003754
4	60.12253144756566
5	58.782086626232925
6	62.942407080338114
7	61.49695119566832
8	60.30658332953146
9	62.554244320722276
10-14	60.81642048384297
15-19	61.47486453527505
20-24	61.43007401788473
25-29	61.301223725866635
30-34	61.79427584729128
35-39	61.561057982809054
40-44	61.55548343623371
45-49	61.91059723922022
50-54	61.649297604130304
55-59	61.58651948760164
60-64	61.925559826235876
65-69	61.6493079767427
70-74	61.61739309505283
75-79	61.95037582421307
80-84	61.693222651896974
85-89	61.649012638693925
90-94	61.955876008638676
95-99	61.76665402406265
100-101	61.514740363924524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	21.5
2	7.5
3	6.5
4	5.5
5	7.0
6	8.0
7	5.5
8	6.0
9	6.5
10	7.0
11	7.0
12	7.5
13	12.5
14	17.0
15	20.5
16	24.0
17	26.5
18	34.0
19	42.5
20	56.5
21	67.5
22	79.0
23	96.0
24	116.5
25	143.0
26	184.5
27	254.5
28	299.5
29	328.5
30	386.0
31	441.0
32	514.5
33	627.0
34	707.0
35	813.5
36	948.5
37	1084.0
38	1224.5
39	1423.0
40	1616.5
41	1823.5
42	2196.0
43	2633.0
44	3070.5
45	3750.5
46	4706.0
47	5766.0
48	7189.5
49	8881.0
50	10673.5
51	12882.5
52	15344.5
53	17834.5
54	20450.0
55	23247.5
56	26016.5
57	28621.5
58	31053.0
59	33013.5
60	34666.0
61	36299.5
62	37301.5
63	37798.0
64	38046.0
65	37713.5
66	36801.5
67	35245.5
68	32961.5
69	30163.5
70	27422.0
71	24206.5
72	20429.0
73	16948.0
74	13843.5
75	10856.0
76	8242.0
77	6033.5
78	4174.5
79	2837.5
80	1803.0
81	1094.0
82	668.0
83	361.5
84	195.5
85	107.0
86	51.5
87	35.5
88	23.5
89	14.5
90	13.0
91	12.0
92	6.5
93	5.0
94	5.5
95	1.5
96	1.5
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015118990364250367
2	0.00977520928723084
3	0.011860587268506752
4	0.012642604011485217
5	0.008602184172763138
6	0.006777478439146715
7	0.007950503553614418
8	0.01068756215403905
9	0.015118990364250367
10-14	0.005135243278891935
15-19	0.004718167682636752
20-24	0.0
25-29	0.0
30-34	0.0017986385088504745
35-39	7.559495182125183E-4
40-44	0.0
45-49	0.0
50-54	2.0853779812759127E-4
55-59	2.6067224765948908E-5
60-64	0.0
65-69	5.213444953189781E-4
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	767247.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.10246157690865863
#Duplication Level	Relative count
1	100.0
2	0.06404162705758744
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.003252113874019E-4
10++	5.003252113874019E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	65355	3.2194104	4.1814666	7
TTTTT	59190	3.1088123	3.6479402	10-14
>>END_MODULE