##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56542925	34.0	31.0	34.0	31.0	34.0
2	32.7074855	34.0	31.0	34.0	31.0	34.0
3	32.75306575	34.0	31.0	34.0	31.0	34.0
4	36.1295735	37.0	37.0	37.0	35.0	37.0
5	36.14388575	37.0	37.0	37.0	35.0	37.0
6	36.1401715	37.0	37.0	37.0	35.0	37.0
7	36.11603475	37.0	37.0	37.0	35.0	37.0
8	36.12272375	37.0	37.0	37.0	35.0	37.0
9	37.877168	39.0	39.0	39.0	35.0	39.0
10-14	38.1007453	39.4	38.4	39.4	35.2	39.4
15-19	39.261700700000006	41.0	39.6	41.0	36.0	41.0
20-24	39.063701300000005	41.0	39.0	41.0	35.6	41.0
25-29	38.68153475	40.2	38.4	41.0	35.0	41.0
30-34	38.06007895	40.0	37.4	41.0	33.4	41.0
35-39	37.1028751	39.0	35.2	40.8	32.6	41.0
40-44	36.1633457	37.0	35.0	40.0	31.8	41.0
45-49	35.19142785	35.2	35.0	39.0	30.8	41.0
50-54	33.867633049999995	34.8	33.4	36.2	29.0	39.2
55-59	33.932175099999995	35.0	35.0	35.0	31.0	38.2
60-64	33.52256179999999	35.0	34.4	35.0	30.0	36.4
65-69	33.214619549999995	35.0	34.0	35.0	29.8	35.0
70-74	32.942789700000006	35.0	34.0	35.0	29.0	35.0
75-79	32.688396250000004	35.0	33.0	35.0	29.0	35.0
80-84	32.44375240000001	35.0	33.0	35.0	27.4	35.0
85-89	32.1464018	35.0	33.0	35.0	27.0	35.0
90-94	31.7790032	35.0	33.0	35.0	25.8	35.0
95-99	31.2634865	35.0	33.0	35.0	24.0	35.0
100-101	30.218576374999998	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6391.0
3	1224.0
4	1665.0
5	1868.0
6	2556.0
7	3308.0
8	4064.0
9	4515.0
10	5030.0
11	5231.0
12	5264.0
13	5290.0
14	5194.0
15	5272.0
16	5630.0
17	6432.0
18	7050.0
19	8175.0
20	9461.0
21	11139.0
22	13693.0
23	16592.0
24	20339.0
25	25036.0
26	30020.0
27	36476.0
28	44459.0
29	54162.0
30	67288.0
31	85624.0
32	112487.0
33	156706.0
34	254112.0
35	614356.0
36	1411292.0
37	827164.0
38	115155.0
39	10215.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.28705030967923	24.428135355266797	13.565771626387601	23.719042708666365
2	30.50025288288996	24.819042406784767	19.148212385608637	25.53249232471664
3	29.391105330298405	19.951074314014857	21.410440481252657	29.247379874434078
4	30.638588349944897	19.847701921398123	19.91383799862825	29.59987173002873
5	31.14926185071939	21.083594389940412	20.058776816922723	27.708366942417477
6	29.33319227193636	19.461395572422894	17.51980049316465	33.68561166247609
7	32.59814380067142	19.01531291910931	19.461879280511408	28.924663999707857
8	28.350549633348464	19.847285853116755	19.865146279140447	31.937018234394326
9	30.515188108591957	18.229708055985643	19.23887963666921	32.01622419875319
10-14	30.45423987678348	19.68817445616991	19.511896487159802	30.345689179886804
15-19	30.666204048932176	19.19072173018099	19.344977102272747	30.79809711861408
20-24	30.598852547279932	19.219600536277355	19.35857539963899	30.822971516803722
25-29	30.741056209112937	19.365700655023133	19.266602321342337	30.626640814521593
30-34	30.901939094251052	19.13138525088743	19.08052717338378	30.886148481477743
35-39	30.74905034336673	19.186942386955792	19.201925844388594	30.862081425288885
40-44	30.858887011925674	19.280948525561612	19.135527766899568	30.724636695613146
45-49	30.981934550830964	19.05729598757424	19.008117178675487	30.952652282919313
50-54	30.794918259155065	19.112396093385847	19.158417621539613	30.934268025919476
55-59	30.902754910245676	19.218443218252993	19.12521119598372	30.753590675517607
60-64	31.013845	19.033045	18.964765	30.988345
65-69	30.799876219733875	19.125191119160906	19.146691165386006	30.928241495719217
70-74	30.89927	19.219565	19.10446	30.776705
75-79	31.00817077925656	19.04699475584913	18.980109107058336	30.96472535783597
80-84	30.82219846503646	19.124752875850337	19.12238782417396	30.930660834939243
85-89	30.944505	19.21469	19.102435	30.73837
90-94	31.011875	19.032384999999998	18.969345	30.986395
95-99	30.872995134893678	19.095569824240773	19.110184843240297	30.921250197625255
100-101	31.016939173700127	19.30698050756896	19.10711743296262	30.568962885768297
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.006093018345595
2	56.032745207606595
3	58.63848520473248
4	60.23846007997362
5	58.857628793136875
6	63.018803934412446
7	61.52280780037928
8	60.287567867742794
9	62.53141230734515
10-14	60.79992905667029
15-19	61.46430116754627
20-24	61.42182406408365
25-29	61.36769702363453
30-34	61.78808757572879
35-39	61.61113176865561
40-44	61.58352370753882
45-49	61.934586833750274
50-54	61.72918628507453
55-59	61.65634558576328
60-64	62.00219
65-69	61.72811771545309
70-74	61.67597500000001
75-79	61.972896137092526
80-84	61.752859299975704
85-89	61.682875
90-94	61.99827
95-99	61.79424533251894
100-101	61.585902059468424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	68.5
2	22.5
3	17.5
4	21.0
5	23.0
6	23.5
7	24.5
8	26.0
9	36.5
10	42.5
11	47.5
12	63.5
13	78.5
14	88.5
15	101.5
16	122.5
17	160.0
18	189.0
19	242.0
20	312.5
21	381.0
22	466.5
23	583.0
24	721.5
25	865.0
26	1017.5
27	1208.0
28	1423.5
29	1723.0
30	2041.5
31	2319.5
32	2739.0
33	3278.5
34	3819.0
35	4345.0
36	4914.0
37	5659.0
38	6471.5
39	7348.5
40	8418.5
41	9644.5
42	11321.0
43	13519.5
44	16335.0
45	20024.5
46	24509.0
47	30113.5
48	36999.5
49	45602.0
50	55825.5
51	66846.5
52	79189.0
53	92854.0
54	107246.0
55	121524.5
56	135407.0
57	148437.5
58	160457.5
59	171598.5
60	180971.5
61	188085.0
62	193722.5
63	197296.0
64	197610.5
65	195872.5
66	190929.5
67	182506.5
68	171832.5
69	158462.5
70	142907.0
71	125757.0
72	107522.0
73	89409.0
74	72566.0
75	57216.5
76	43333.5
77	31575.5
78	22372.5
79	15462.0
80	10145.5
81	6262.0
82	3723.0
83	2113.0
84	1210.0
85	682.5
86	411.0
87	284.5
88	194.5
89	143.0
90	111.0
91	84.5
92	62.0
93	47.5
94	41.5
95	34.0
96	26.0
97	20.0
98	13.0
99	9.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06595000000000001
2	0.052550000000000006
3	0.0525
4	0.05455
5	0.045625
6	0.043525
7	0.04845
8	0.058374999999999996
9	0.069025
10-14	0.032885
15-19	0.032654999999999997
20-24	0.010695
25-29	0.013454999999999998
30-34	0.035545
35-39	0.023075
40-44	0.00396
45-49	0.007745
50-54	0.014185
55-59	0.012894999999999998
60-64	0.0
65-69	2.1500000000000002E-4
70-74	0.0
75-79	9.7E-4
80-84	0.002185
85-89	0.0
90-94	0.0
95-99	1.3000000000000002E-4
100-101	2.875E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.25387813849353696
#Duplication Level	Relative count
1	100.0
2	0.1979076898410726
3	0.0035072248832595148
4	0.001503096378539792
5	0.0
6	0.001503096378539792
7	5.010321261799306E-4
8	0.0
9	0.0
10++	0.001503096378539792
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	332105	3.1740713	3.9510102	1
TTTTT	313390	3.1552234	3.741234	10-14
>>END_MODULE