##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60223725	34.0	31.0	34.0	31.0	34.0
2	32.775428	34.0	31.0	34.0	31.0	34.0
3	32.829846	34.0	31.0	34.0	31.0	34.0
4	36.17083325	37.0	37.0	37.0	35.0	37.0
5	36.195739	37.0	37.0	37.0	35.0	37.0
6	36.20577175	37.0	37.0	37.0	35.0	37.0
7	36.1917795	37.0	37.0	37.0	35.0	37.0
8	36.14800675	37.0	37.0	37.0	35.0	37.0
9	37.905144	39.0	39.0	39.0	35.0	39.0
10-14	38.181574749999996	39.4	38.8	39.4	35.4	39.4
15-19	39.2930875	41.0	40.0	41.0	36.2	41.0
20-24	39.1212463	41.0	39.0	41.0	35.8	41.0
25-29	38.7097075	40.2	38.4	41.0	35.0	41.0
30-34	38.142188350000005	40.0	37.4	41.0	34.0	41.0
35-39	37.1466172	39.0	35.2	40.8	32.6	41.0
40-44	36.23020269999999	37.0	35.0	40.0	32.0	41.0
45-49	35.25243135	35.2	35.0	39.0	31.0	41.0
50-54	33.9002262	34.8	33.4	36.2	29.0	39.2
55-59	34.013175950000004	35.0	35.0	35.0	31.0	38.2
60-64	33.60103975	35.0	34.4	35.0	30.6	36.4
65-69	33.286309700000004	35.0	34.0	35.0	30.0	35.0
70-74	33.02357725	35.0	34.0	35.0	29.4	35.0
75-79	32.75288825	35.0	33.4	35.0	29.0	35.0
80-84	32.4811646	35.0	33.0	35.0	27.8	35.0
85-89	32.18442065000001	35.0	33.0	35.0	27.0	35.0
90-94	31.795135200000004	35.0	33.0	35.0	25.8	35.0
95-99	31.256844949999998	35.0	33.0	35.0	24.0	35.0
100-101	30.180515999999997	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6802.0
3	1190.0
4	1617.0
5	1882.0
6	2396.0
7	2975.0
8	3716.0
9	4206.0
10	4344.0
11	4773.0
12	4885.0
13	4858.0
14	4941.0
15	5111.0
16	5345.0
17	6096.0
18	6630.0
19	7731.0
20	8941.0
21	10679.0
22	12906.0
23	15612.0
24	19114.0
25	23218.0
26	27830.0
27	34312.0
28	42283.0
29	52280.0
30	64964.0
31	83946.0
32	111780.0
33	158618.0
34	263734.0
35	640274.0
36	1415984.0
37	812337.0
38	111701.0
39	9916.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.31279328624738	24.460098055702364	13.561085619564695	23.666023038485562
2	30.461775366942568	24.85653345183993	19.12190014918419	25.559791032033313
3	29.38618875922796	19.939395940848996	21.46367909470185	29.210736205221195
4	30.598113910330316	19.86390276324469	19.94650267424091	29.59148065218408
5	31.115173144158653	21.11634373003688	20.090080085082583	27.678403040721882
6	29.321916304131967	19.468675951756747	17.54212730707877	33.66728043703252
7	32.572677695160124	18.974649843789837	19.483849692971404	28.968822768078635
8	28.368458133682388	19.88562175339099	19.864322872020253	31.881597240906366
9	30.55573492392195	18.20171783170175	19.246542737308104	31.9960045070682
10-14	30.440683995970563	19.673558109853904	19.524380069749814	30.361377824425723
15-19	30.677549390093084	19.220707738561693	19.335027763792045	30.76671510755318
20-24	30.59753066168835	19.261989058336287	19.34422532055714	30.796254959418224
25-29	30.78778635559347	19.361188643302537	19.25871128786745	30.592313713236543
30-34	30.88416582052635	19.133629011834326	19.08851042071886	30.89369474692047
35-39	30.76441148194369	19.184283132708778	19.207769031192186	30.843536354155347
40-44	30.853499982972515	19.258108795167775	19.14933793093723	30.73905329092248
45-49	30.994370983073043	19.079667658432268	18.993975550271895	30.93198580822279
50-54	30.772857806804627	19.12804554829784	19.171091036331003	30.92800560856653
55-59	30.889740598177973	19.210152432514068	19.114605186236115	30.785501783071844
60-64	30.99503	19.021469999999997	18.99253	30.99097
65-69	30.793954334608202	19.105810244403028	19.15872546664696	30.941509954341807
70-74	30.91645	19.19962	19.10888	30.775049999999997
75-79	31.018808176321862	19.025305089043297	18.984979389392407	30.970907345242438
80-84	30.82282862480884	19.112221813042567	19.132347452031603	30.932602110116996
85-89	30.915369999999996	19.210745000000003	19.11301	30.760875
90-94	31.032735	19.00685	18.95183	31.008585
95-99	30.850627439913552	19.099561773076015	19.135852346467075	30.91395844054336
100-101	31.034547416444074	19.310679237848753	19.085445003245134	30.569328342462043
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.978816324732946
2	56.02156639897588
3	58.59692496444916
4	60.1895945625144
5	58.793576184880536
6	62.98919674116449
7	61.54150046323876
8	60.25005537458876
9	62.55173943099015
10-14	60.80206182039628
15-19	61.44426449764626
20-24	61.39378562110658
25-29	61.38010006883001
30-34	61.777860567446815
35-39	61.607947836099044
40-44	61.592553273895
45-49	61.92635679129583
50-54	61.70086341537115
55-59	61.67524238124982
60-64	61.986
65-69	61.73546428895001
70-74	61.6915
75-79	61.98971552156431
80-84	61.75543073492583
85-89	61.676244999999994
90-94	62.04132
95-99	61.76458588045691
100-101	61.60387575890611
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	78.5
2	32.5
3	32.5
4	27.5
5	32.0
6	33.5
7	34.5
8	45.5
9	52.5
10	57.5
11	60.0
12	67.0
13	70.0
14	91.5
15	127.0
16	143.5
17	186.0
18	243.0
19	291.0
20	335.0
21	409.0
22	487.5
23	582.0
24	710.5
25	856.5
26	1015.5
27	1219.0
28	1487.5
29	1745.0
30	2059.0
31	2386.5
32	2804.5
33	3335.0
34	3835.0
35	4337.5
36	4900.5
37	5622.0
38	6467.5
39	7286.0
40	8326.0
41	9790.0
42	11506.0
43	13529.5
44	16127.0
45	19605.0
46	24231.5
47	29994.0
48	36845.5
49	45315.5
50	55534.0
51	66729.0
52	79086.5
53	92491.5
54	106960.0
55	121764.5
56	135512.0
57	148985.5
58	161044.0
59	171310.5
60	181166.0
61	189036.0
62	194405.5
63	197559.5
64	198085.5
65	196203.5
66	191156.0
67	183036.5
68	171979.5
69	158578.0
70	142786.5
71	125123.5
72	107024.5
73	88994.5
74	71966.5
75	56472.0
76	43025.5
77	31632.0
78	22419.0
79	15339.5
80	9972.0
81	6174.0
82	3713.0
83	2150.0
84	1207.0
85	706.0
86	458.0
87	307.5
88	224.5
89	178.0
90	131.0
91	97.0
92	81.0
93	69.0
94	54.5
95	42.5
96	36.0
97	22.5
98	19.0
99	18.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09759999999999999
2	0.08995
3	0.0727
4	0.060425
5	0.05005
6	0.04405
7	0.078525
8	0.112125
9	0.11245000000000001
10-14	0.04561
15-19	0.05687999999999999
20-24	0.019775
25-29	0.021814999999999998
30-34	0.041205
35-39	0.025115000000000002
40-44	0.014584999999999999
45-49	0.008295
50-54	0.024365
55-59	0.01805
60-64	0.0
65-69	4.1999999999999996E-4
70-74	0.0
75-79	0.0017350000000000002
80-84	0.003175
85-89	0.0
90-94	0.0
95-99	0.00158
100-101	0.0041
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.24533439928048756
#Duplication Level	Relative count
1	100.0
2	0.16880046482739275
3	0.004007132696199235
4	5.008915870249043E-4
5	5.008915870249043E-4
6	0.0
7	0.0
8	0.001502674761074713
9	0.0
10++	0.003005349522149426
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	317010	3.192249	3.6817932	10-14
AAAAA	328695	3.142965	3.9954503	1
>>END_MODULE