##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55879	34.0	31.0	34.0	31.0	34.0
2	32.72427175	34.0	31.0	34.0	31.0	34.0
3	32.78088125	34.0	31.0	34.0	31.0	34.0
4	36.1430705	37.0	37.0	37.0	35.0	37.0
5	36.15078175	37.0	37.0	37.0	35.0	37.0
6	36.15735525	37.0	37.0	37.0	35.0	37.0
7	36.1438595	37.0	37.0	37.0	35.0	37.0
8	36.129956	37.0	37.0	37.0	35.0	37.0
9	37.88282425	39.0	39.0	39.0	35.0	39.0
10-14	38.10660145	39.4	38.6	39.4	35.2	39.4
15-19	39.096462499999994	41.0	39.6	41.0	35.6	41.0
20-24	38.59834085000001	40.4	38.4	41.0	34.4	41.0
25-29	38.630979	40.0	38.0	41.0	35.0	41.0
30-34	38.061414049999996	40.0	37.4	41.0	33.6	41.0
35-39	37.0389534	39.0	35.2	40.8	32.6	41.0
40-44	36.13872955000001	37.0	35.0	40.0	31.8	41.0
45-49	35.21268425	35.2	35.0	39.0	31.0	41.0
50-54	33.88879975	34.8	33.4	36.2	29.2	39.2
55-59	33.940862550000006	35.0	35.0	35.0	31.0	38.2
60-64	33.525167599999996	35.0	34.4	35.0	30.4	36.4
65-69	33.1916102	35.0	34.0	35.0	29.8	35.0
70-74	32.9281189	35.0	34.0	35.0	29.0	35.0
75-79	32.53284445	35.0	33.0	35.0	28.2	35.0
80-84	32.3250762	35.0	33.0	35.0	27.0	35.0
85-89	32.0362821	35.0	33.0	35.0	27.0	35.0
90-94	31.3675004	35.0	32.6	35.0	23.4	35.0
95-99	30.9131781	35.0	32.2	35.0	22.8	35.0
100-101	29.333417125	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8000.0
3	1300.0
4	1831.0
5	2151.0
6	2703.0
7	3225.0
8	3951.0
9	4429.0
10	4724.0
11	4973.0
12	5261.0
13	5044.0
14	5178.0
15	5498.0
16	5827.0
17	6338.0
18	7140.0
19	8150.0
20	9549.0
21	11335.0
22	13966.0
23	16802.0
24	20375.0
25	24710.0
26	29875.0
27	36934.0
28	44546.0
29	55638.0
30	69406.0
31	89137.0
32	118173.0
33	170026.0
34	290984.0
35	692379.0
36	1369242.0
37	741766.0
38	100728.0
39	8647.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.302542280578535	24.43886921282821	13.568719946017069	23.689868560576187
2	30.45563723250729	24.883853372000896	19.15045678874819	25.510052606743628
3	29.39064933868843	19.916605267267578	21.44054502644846	29.25220036759553
4	30.62523258930616	19.85065733147771	19.949837040455463	29.574273038760673
5	31.123278798967625	21.076802836157405	20.10060991098468	27.699308453890293
6	29.300863314535462	19.500472281479762	17.498629182993064	33.70003522099171
7	32.56500815260638	19.014090146106316	19.501795174898177	28.919106526389122
8	28.34555146282148	19.856821610970883	19.907558052801942	31.890068873405696
9	30.53653066613603	18.20957873250559	19.253830529347056	32.00006007201132
10-14	30.470286004210745	19.679615686516623	19.505531300592725	30.344567008679903
15-19	30.87912521745248	19.21014744712903	19.28071896080544	30.63000837461305
20-24	30.676207038800605	19.336300668783448	19.24877422714947	30.738718065266475
25-29	30.722763139795166	19.389233058636687	19.27651656308227	30.611487238485875
30-34	30.889874239917987	19.14037620010547	19.08806017408624	30.881689385890304
35-39	30.775174813701454	19.213645472931386	19.208728399893396	30.80245131347376
40-44	30.85841016355217	19.274812568078794	19.14225758822364	30.724519680145402
45-49	30.976272642686336	19.049950501845522	19.001775132097183	30.97200172337096
50-54	30.774943023676936	19.133104494276356	19.175273976577177	30.916678505469523
55-59	30.865785660929866	19.23564260795598	19.130337876480795	30.768233854633355
60-64	31.005417904876115	19.021252119126906	18.975142077627872	30.998187898369107
65-69	30.787306180959273	19.139828709743064	19.149903724855587	30.922961384442075
70-74	30.917365000000004	19.212605	19.121895	30.748134999999998
75-79	31.064483894627383	19.041878189087395	18.987094540496397	30.906543375788825
80-84	30.80580676939694	19.117773104936404	19.154883022000416	30.921537103666235
85-89	30.917804999999998	19.206345	19.12214	30.75371
90-94	31.065331642728346	19.08768130865986	18.941305876852336	30.905681171759458
95-99	30.864673863719037	19.085554816332152	19.142519188347705	30.907252131601098
100-101	31.143646126323194	19.421513693374777	19.070404084238803	30.36443609606323
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.99241084115472
2	55.965689839250906
3	58.64284970628396
4	60.19950562806683
5	58.822587252857915
6	63.00089853552717
7	61.4841146789955
8	60.23562033622717
9	62.53659073814736
10-14	60.814853012890644
15-19	61.509133592065524
20-24	61.41492510406707
25-29	61.33425037828104
30-34	61.77156362580829
35-39	61.57762612717522
40-44	61.58292984369756
45-49	61.9482743660573
50-54	61.69162152914647
55-59	61.634019515563224
60-64	62.00360580324522
65-69	61.710267565401345
70-74	61.665499999999994
75-79	61.971027270416215
80-84	61.72734387306318
85-89	61.671515
90-94	61.9710128144878
95-99	61.771925995320146
100-101	61.50808222238642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	73.0
2	38.0
3	39.5
4	33.5
5	31.5
6	37.0
7	35.5
8	39.0
9	50.5
10	63.0
11	76.0
12	98.5
13	117.5
14	131.5
15	167.0
16	205.0
17	253.5
18	304.5
19	353.0
20	390.0
21	425.0
22	533.5
23	652.5
24	766.0
25	910.0
26	1048.5
27	1247.5
28	1489.0
29	1761.0
30	2104.5
31	2422.5
32	2775.5
33	3264.5
34	3792.0
35	4368.5
36	4981.0
37	5600.0
38	6382.5
39	7350.0
40	8356.5
41	9689.5
42	11355.0
43	13499.5
44	16184.5
45	19658.5
46	24360.5
47	30018.0
48	37063.0
49	45430.5
50	55466.0
51	67065.5
52	79482.5
53	93044.5
54	107421.5
55	121549.5
56	135283.5
57	148584.5
58	161122.0
59	171955.0
60	181391.0
61	189162.5
62	194477.0
63	197612.0
64	198144.5
65	195854.5
66	190791.0
67	183051.5
68	172238.0
69	158018.0
70	142076.5
71	125097.5
72	106825.5
73	88607.0
74	71673.0
75	56281.5
76	42705.0
77	31435.0
78	22152.5
79	15092.5
80	9921.5
81	6241.5
82	3771.0
83	2236.0
84	1325.5
85	789.0
86	513.5
87	341.5
88	253.5
89	194.5
90	170.5
91	127.5
92	82.0
93	71.0
94	63.5
95	48.0
96	31.0
97	25.0
98	22.5
99	16.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.153625
2	0.131625
3	0.10759999999999999
4	0.105575
5	0.104275
6	0.05959999999999999
7	0.09330000000000001
8	0.1211
9	0.119875
10-14	0.062835
15-19	0.072995
20-24	0.041635
25-29	0.04125
30-34	0.059305000000000004
35-39	0.04216
40-44	0.037704999999999995
45-49	0.021525
50-54	0.0462
55-59	0.03773
60-64	8.999999999999999E-5
65-69	1.5000000000000001E-4
70-74	0.0
75-79	0.006660000000000001
80-84	0.01325
85-89	0.0
90-94	2.95E-4
95-99	0.0076749999999999995
100-101	0.0205375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.19991403696410126
#Duplication Level	Relative count
1	100.0
2	0.15374137395711265
3	0.003004717406327935
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007862343879892E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	340740	3.2399917	4.2334166	1
TTTTT	319365	3.2124457	3.7382543	10-14
>>END_MODULE