##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.515384	34.0	31.0	34.0	31.0	34.0
2	32.68351825	34.0	31.0	34.0	31.0	34.0
3	32.7256805	34.0	31.0	34.0	31.0	34.0
4	36.095267	37.0	37.0	37.0	35.0	37.0
5	36.09443675	37.0	37.0	37.0	35.0	37.0
6	36.115443	37.0	37.0	37.0	35.0	37.0
7	36.12021175	37.0	37.0	37.0	35.0	37.0
8	36.123543	37.0	37.0	37.0	35.0	37.0
9	37.853705	39.0	39.0	39.0	35.0	39.0
10-14	38.12592514999999	39.4	38.4	39.4	35.4	39.4
15-19	39.21042475	41.0	39.2	41.0	36.0	41.0
20-24	38.986693249999995	41.0	39.0	41.0	35.6	41.0
25-29	38.677370399999994	40.2	38.4	41.0	35.0	41.0
30-34	38.017717649999994	40.0	37.4	41.0	33.6	41.0
35-39	37.06563645	39.0	35.2	40.8	32.6	41.0
40-44	36.1140743	37.0	35.0	40.0	31.4	41.0
45-49	35.173562	35.2	35.0	39.0	30.8	41.0
50-54	33.836128900000006	34.8	33.4	36.2	29.0	39.2
55-59	33.90974575	35.0	35.0	35.0	30.8	38.2
60-64	33.4851548	35.0	34.0	35.0	30.0	36.4
65-69	33.1646738	35.0	34.0	35.0	29.8	35.0
70-74	32.901580349999996	35.0	34.0	35.0	29.0	35.0
75-79	32.6020036	35.0	33.0	35.0	28.2	35.0
80-84	32.320909549999996	35.0	33.0	35.0	27.0	35.0
85-89	32.0634253	35.0	33.0	35.0	27.0	35.0
90-94	31.650359200000004	35.0	33.0	35.0	25.0	35.0
95-99	31.09662985	35.0	32.8	35.0	23.6	35.0
100-101	29.962602875	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7441.0
3	1382.0
4	1690.0
5	2105.0
6	2745.0
7	3416.0
8	4251.0
9	4631.0
10	4861.0
11	5360.0
12	5317.0
13	5311.0
14	5378.0
15	5452.0
16	5811.0
17	6457.0
18	7181.0
19	8257.0
20	9654.0
21	11522.0
22	13597.0
23	16652.0
24	20072.0
25	24610.0
26	30014.0
27	36590.0
28	44751.0
29	55237.0
30	69602.0
31	88220.0
32	116320.0
33	164458.0
34	269032.0
35	630801.0
36	1383680.0
37	806188.0
38	111775.0
39	10101.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.29700442822347	24.43320468525236	13.55219793780542	23.717592948718753
2	30.48565268486802	24.859988318166653	19.155752100676164	25.498606896289168
3	29.34831926586759	19.957371922960967	21.440490463269843	29.2538183479016
4	30.56637675264572	19.882444815234287	19.91938743870655	29.631790993413443
5	31.07837926251144	21.105621663269297	20.113889065617716	27.702110008601544
6	29.32022821431501	19.486212718627034	17.495688973975806	33.69787009308215
7	32.59520531056045	19.02515683949229	19.451173063870673	28.928464786076585
8	28.328735603436417	19.895406855327824	19.87707429314592	31.898783248089835
9	30.552194113421965	18.245955157291725	19.25492985336718	31.946920875919126
10-14	30.445222547263345	19.687208734443626	19.506964769238415	30.360603949054614
15-19	30.700255670325795	19.246730115044148	19.323426212383886	30.729588002246178
20-24	30.611016676046283	19.254679410941872	19.340773521935674	30.793530391076175
25-29	30.717052340103297	19.391295891247264	19.27355009646315	30.618101672186288
30-34	30.88690399987198	19.151237407884526	19.084856255850983	30.87700233639251
35-39	30.727968574700338	19.220435604926116	19.216235538145057	30.835360282228486
40-44	30.84615211538029	19.276043190107973	19.150952877382192	30.726851817129543
45-49	30.97650548825274	19.049894524947263	19.00565950282975	30.967940483970242
50-54	30.776086725971957	19.135718361897197	19.176684482320592	30.911510429810257
55-59	30.876238783175967	19.22550188831246	19.139256319091704	30.75900300941986
60-64	30.972624999999997	19.040185	18.996595	30.990595
65-69	30.795384999999996	19.11989	19.170005	30.914720000000003
70-74	30.900395000000003	19.217805	19.12314	30.75866
75-79	31.01277	19.034875	18.9769	30.975455
80-84	30.81159116231823	19.122413824482763	19.130093826018765	30.935901187180235
85-89	30.916715	19.204935	19.10294	30.775409999999997
90-94	31.04477	19.0351	18.982105	30.938025000000003
95-99	30.849749999999997	19.088675	19.14015	30.921425000000003
100-101	31.021193015403725	19.352428209836887	19.101576547944628	30.52480222681475
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.01459737694221
2	55.98425958115718
3	58.60213761376919
4	60.19816774605916
5	58.78048927111299
6	63.01809830739716
7	61.52367009663704
8	60.22751885152625
9	62.499114989341095
10-14	60.80582649631796
15-19	61.42984367257197
20-24	61.40454706712245
25-29	61.335154012289586
30-34	61.7639063362645
35-39	61.56332885692882
40-44	61.57300393250983
45-49	61.94444597222299
50-54	61.687597155782214
55-59	61.63524179259583
60-64	61.96322
65-69	61.710105000000006
70-74	61.659054999999995
75-79	61.988225
80-84	61.747492349498465
85-89	61.692125
90-94	61.982795
95-99	61.771175
100-101	61.545995242218474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	105.0
2	50.5
3	39.0
4	35.5
5	27.5
6	37.5
7	54.5
8	56.5
9	58.0
10	59.5
11	75.5
12	92.0
13	102.0
14	108.0
15	130.0
16	161.0
17	190.0
18	234.0
19	272.5
20	320.0
21	397.5
22	482.0
23	598.0
24	715.0
25	841.0
26	1035.0
27	1214.0
28	1432.0
29	1727.0
30	2049.0
31	2431.5
32	2885.0
33	3279.5
34	3749.5
35	4395.5
36	4973.0
37	5552.0
38	6348.0
39	7246.0
40	8270.0
41	9722.5
42	11483.0
43	13549.5
44	16314.0
45	19893.0
46	24380.0
47	30103.0
48	37156.0
49	45595.5
50	55639.5
51	66917.0
52	79545.5
53	93341.0
54	107510.0
55	121604.0
56	135678.0
57	149137.0
58	161386.5
59	171724.5
60	180709.0
61	188228.0
62	194099.0
63	197389.5
64	197448.0
65	195628.0
66	190980.5
67	182706.0
68	171542.5
69	158146.5
70	142085.5
71	124757.0
72	106960.0
73	89140.5
74	72218.0
75	56611.0
76	43028.0
77	31731.5
78	22502.5
79	15313.0
80	10016.0
81	6236.0
82	3770.5
83	2243.0
84	1327.5
85	790.0
86	479.5
87	322.5
88	233.0
89	168.0
90	124.0
91	98.0
92	85.5
93	73.0
94	65.0
95	53.0
96	39.5
97	26.0
98	20.0
99	14.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22522499999999998
2	0.1869
3	0.15969999999999998
4	0.18305000000000002
5	0.19235000000000002
6	0.0238
7	0.03315
8	0.04125
9	0.071825
10-14	0.01607
15-19	0.007949999999999999
20-24	0.004775000000000001
25-29	6.749999999999999E-4
30-34	0.0168
35-39	0.00159
40-44	2.5E-4
45-49	4.9999999999999996E-5
50-54	0.002735
55-59	6.6E-4
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	1.9999999999999998E-5
85-89	0.0
90-94	0.0
95-99	0.0
100-101	6.625E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.23838319223580184
#Duplication Level	Relative count
1	100.0
2	0.1863531391987817
3	0.0015028478967643686
4	0.0
5	5.009492989214562E-4
6	0.0
7	0.0
8	0.0
9	5.009492989214562E-4
10++	0.0010018985978429123
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	345745	3.3031306	4.257626	1
TTTTT	323135	3.2483425	3.750197	10-14
>>END_MODULE