##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002006_Sedgewick_ACTGAT_L002_R2_004.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 61 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5948245 34.0 31.0 34.0 31.0 34.0 2 32.77389125 34.0 31.0 34.0 31.0 34.0 3 32.793975 34.0 31.0 34.0 31.0 34.0 4 36.1885245 37.0 37.0 37.0 35.0 37.0 5 36.19646175 37.0 37.0 37.0 35.0 37.0 6 36.183221 37.0 37.0 37.0 35.0 37.0 7 36.178148 37.0 37.0 37.0 35.0 37.0 8 36.1596235 37.0 37.0 37.0 35.0 37.0 9 37.93034375 39.0 39.0 39.0 37.0 39.0 10-14 38.223032 39.4 39.0 39.4 36.2 39.4 15-19 39.394045950000006 41.0 40.0 41.0 36.8 41.0 20-24 39.20789130000001 41.0 39.2 41.0 36.0 41.0 25-29 38.842779300000004 40.4 38.6 41.0 35.0 41.0 30-34 38.2235433 40.0 37.4 41.0 34.2 41.0 35-39 37.304065800000004 39.0 35.4 41.0 33.0 41.0 40-44 36.34661625 37.0 35.0 40.0 32.2 41.0 45-49 35.42838239999999 35.2 35.0 39.0 31.4 41.0 50-54 34.1211078 34.8 33.8 36.2 29.8 39.4 55-59 34.19702345 35.0 35.0 35.2 31.0 38.2 60-64 33.7622512 35.0 35.0 35.0 31.0 36.4 65-69 33.430156849999996 35.0 34.2 35.0 30.6 35.0 70-74 33.186212299999994 35.0 34.0 35.0 30.0 35.0 75-79 32.989293450000005 35.0 34.0 35.0 29.4 35.0 80-84 32.75644485 35.0 33.4 35.0 29.0 35.0 85-89 32.48492845 35.0 33.0 35.0 28.2 35.0 90-94 32.16546495 35.0 33.0 35.0 27.0 35.0 95-99 31.696931499999998 35.0 33.0 35.0 26.2 35.0 100-101 30.684328625 34.0 31.5 35.0 22.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6189.0 3 906.0 4 1186.0 5 1527.0 6 1909.0 7 2492.0 8 3091.0 9 3556.0 10 3687.0 11 3990.0 12 4039.0 13 3995.0 14 4032.0 15 4421.0 16 4822.0 17 5204.0 18 5952.0 19 6807.0 20 7816.0 21 9378.0 22 11497.0 23 14016.0 24 17390.0 25 21498.0 26 26093.0 27 32318.0 28 39919.0 29 49337.0 30 62049.0 31 79406.0 32 105280.0 33 148954.0 34 243731.0 35 609572.0 36 1463019.0 37 861309.0 38 118917.0 39 10590.0 40 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.35366189496242 24.4256680636294 13.523750869972313 23.69691917143587 2 30.49697144418719 24.82519862541304 19.14006232288379 25.53776760751598 3 29.411742624541375 19.940534359913524 21.38771011739221 29.260012898152894 4 30.622632193633166 19.88480627499499 19.854451307052887 29.638110224318957 5 31.142115223581477 21.06644603577644 20.074207658314425 27.717231082327654 6 29.356805275728213 19.439899597699682 17.502968548658906 33.700326577913195 7 32.61434183455767 18.97302692599708 19.47499714533548 28.937634094109775 8 28.36792102941438 19.847124461864134 19.84101404819163 31.943940460529856 9 30.57568240449942 18.158704613190615 19.263230475259583 32.00238250705038 10-14 30.455646547111233 19.641672070197547 19.502942233827174 30.399739148864047 15-19 30.69501262253168 19.178797089168985 19.338822437816724 30.78736785048261 20-24 30.604231732309135 19.1909584513078 19.352769162392434 30.852040653990624 25-29 30.750208270136838 19.35339020655304 19.26120649935912 30.635195023951 30-34 30.907957195529335 19.10639394020228 19.081340135329583 30.9043087289388 35-39 30.737325337457055 19.172791351989186 19.213303760659272 30.87657954989448 40-44 30.867727417300973 19.239152763306706 19.132155041259796 30.760964778132532 45-49 30.9977573428114 19.02728946425345 18.99796564100317 30.976987551931977 50-54 30.803165913439877 19.100251457968024 19.15785890182815 30.938723726763946 55-59 30.89503769135828 19.195273692568506 19.128429361319974 30.78125925475324 60-64 31.01954528209018 18.98820975048095 18.97514969562872 31.017095271800144 65-69 30.80557808055781 19.104976910497694 19.13403191340319 30.955413095541314 70-74 30.901885 19.209580000000003 19.093945 30.79459 75-79 31.01155635509522 19.031882610882374 18.956505728973056 31.00005530504935 80-84 30.817732539618483 19.104217232782116 19.12641156289592 30.951638664703484 85-89 30.93327243866641 19.219345903675293 19.073422707957306 30.773958949700997 90-94 31.05267163175518 19.00891363922001 18.940848339732693 30.997566389292114 95-99 30.84185354406946 19.085715434420862 19.12124961501721 30.951181406492474 100-101 30.97914334021604 19.307412573004807 19.086366584537558 30.62707750224159 >>END_MODULE >>Per base GC content warn #Base %GC 1 62.050581066398294 2 56.03473905170316 3 58.67175552269427 4 60.260742417952116 5 58.859346305909135 6 63.057131853641415 7 61.551975928667446 8 60.31186148994424 9 62.5780649115498 10-14 60.85538569597527 15-19 61.482380473014295 20-24 61.45627238629976 25-29 61.38540329408784 30-34 61.812265924468136 35-39 61.61390488735153 40-44 61.6286921954335 45-49 61.97474489474338 50-54 61.74188964020383 55-59 61.67629694611152 60-64 62.03664055389032 65-69 61.760991176099125 70-74 61.69647499999999 75-79 62.011611660144574 80-84 61.76937120432196 85-89 61.707231388367404 90-94 62.050238021047285 95-99 61.79303495056193 100-101 61.60622084245764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 11.0 2 7.0 3 8.0 4 5.5 5 8.5 6 11.5 7 17.0 8 20.0 9 22.0 10 43.0 11 64.5 12 85.5 13 115.5 14 141.0 15 180.0 16 206.0 17 236.0 18 290.5 19 329.5 20 370.0 21 442.0 22 525.0 23 627.0 24 749.0 25 853.5 26 997.0 27 1220.5 28 1468.0 29 1727.5 30 2024.0 31 2386.0 32 2789.0 33 3250.5 34 3744.0 35 4297.5 36 4911.0 37 5561.5 38 6369.5 39 7301.0 40 8381.0 41 9655.5 42 11284.0 43 13338.5 44 16017.0 45 19561.0 46 24013.0 47 29720.5 48 36735.5 49 45162.0 50 55254.5 51 66719.5 52 79126.5 53 92537.0 54 107098.5 55 121955.5 56 135692.0 57 148649.0 58 160669.5 59 171177.0 60 180958.5 61 188482.5 62 193366.0 63 197003.0 64 198185.0 65 196531.0 66 191574.5 67 183279.0 68 172322.0 69 158689.0 70 142756.5 71 125488.5 72 107752.5 73 90005.5 74 73104.5 75 57317.5 76 43350.5 77 31785.0 78 22357.0 79 15054.0 80 9837.5 81 6165.5 82 3607.5 83 2004.5 84 1103.0 85 585.0 86 333.0 87 201.5 88 154.0 89 115.0 90 77.0 91 59.0 92 48.0 93 42.0 94 35.5 95 29.0 96 19.0 97 16.0 98 14.0 99 12.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1405 2 0.10089999999999999 3 0.10197500000000001 4 0.09872500000000001 5 0.10464999999999999 6 0.16165000000000002 7 0.1634 8 0.170425 9 0.1472 10-14 0.11161000000000001 15-19 0.081455 20-24 0.05606 25-29 0.063685 30-34 0.095015 35-39 0.067655 40-44 0.035254999999999995 45-49 0.04714 50-54 0.03896 55-59 0.05136 60-64 4.1999999999999996E-4 65-69 9.999999999999999E-6 70-74 0.0 75-79 0.00913 80-84 0.01951 85-89 0.00219 90-94 4.4E-4 95-99 0.011765 100-101 0.01515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.3096547349705128 #Duplication Level Relative count 1 100.0 2 0.18536795539145204 3 0.0030059668441857084 4 0.002003977896123806 5 5.009944740309515E-4 6 0.0015029834220928542 7 5.009944740309515E-4 8 5.009944740309515E-4 9 5.009944740309515E-4 10++ 0.005009944740309515 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 302830 3.0619218 3.7583282 10-14 >>END_MODULE