##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002006_Sedgewick_ACTGAT_L002_R2_003.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 61 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69155175 34.0 31.0 34.0 31.0 34.0 2 32.8674895 34.0 31.0 34.0 31.0 34.0 3 32.897894 34.0 33.0 34.0 31.0 34.0 4 36.26029475 37.0 37.0 37.0 35.0 37.0 5 36.26127675 37.0 37.0 37.0 35.0 37.0 6 36.249597 37.0 37.0 37.0 35.0 37.0 7 36.2398885 37.0 37.0 37.0 35.0 37.0 8 36.2180755 37.0 37.0 37.0 35.0 37.0 9 37.9974365 39.0 39.0 39.0 37.0 39.0 10-14 38.30564815 39.4 39.2 39.4 37.0 39.4 15-19 39.4915239 41.0 40.0 41.0 37.0 41.0 20-24 39.29656355 41.0 39.6 41.0 36.4 41.0 25-29 38.93445385 40.6 38.8 41.0 35.2 41.0 30-34 38.29628275 40.0 37.6 41.0 34.6 41.0 35-39 37.3659046 39.2 35.4 41.0 33.0 41.0 40-44 36.41329455 37.4 35.0 40.0 32.8 41.0 45-49 35.4820375 35.2 35.0 39.0 31.6 41.0 50-54 34.1653057 35.0 34.0 36.2 29.8 39.4 55-59 34.21490415 35.0 35.0 35.2 31.0 38.2 60-64 33.7921051 35.0 35.0 35.0 31.0 36.4 65-69 33.45487575 35.0 34.4 35.0 31.0 35.2 70-74 33.19073285 35.0 34.0 35.0 30.0 35.0 75-79 32.95911135 35.0 34.0 35.0 29.4 35.0 80-84 32.6825751 35.0 33.0 35.0 29.0 35.0 85-89 32.3982794 35.0 33.0 35.0 27.8 35.0 90-94 32.0710781 35.0 33.0 35.0 27.0 35.0 95-99 31.547273 35.0 33.0 35.0 25.0 35.0 100-101 30.448948 34.0 31.5 35.0 21.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6868.0 3 1014.0 4 1367.0 5 1631.0 6 2057.0 7 2658.0 8 3045.0 9 3490.0 10 3934.0 11 4061.0 12 4147.0 13 4080.0 14 4189.0 15 4487.0 16 4857.0 17 5353.0 18 6039.0 19 6768.0 20 7933.0 21 9598.0 22 11358.0 23 13720.0 24 16639.0 25 20791.0 26 24823.0 27 30604.0 28 37487.0 29 47129.0 30 59469.0 31 76969.0 32 101898.0 33 146262.0 34 243948.0 35 616363.0 36 1464852.0 37 869125.0 38 120087.0 39 10833.0 40 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.33333249850859 24.433275045869447 13.569241449850159 23.66415100577181 2 30.5191817879641 24.850372242223763 19.09903965911503 25.531406310697108 3 29.385636523617663 19.911860087589194 21.439226391407797 29.26327699738534 4 30.65246722121988 19.863631665343977 19.891762264403106 29.592138849033034 5 31.110577235894432 21.082248482209444 20.085461743669825 27.7217125382263 6 29.365790456882486 19.411821269631083 17.486274894751656 33.73611337873478 7 32.57766216800384 19.004483635556717 19.460310733826528 28.95754346261291 8 28.393076316204173 19.829916829911067 19.888917811757555 31.888089042127206 9 30.5768641571326 18.20868960054232 19.175712227534028 32.03873401479105 10-14 30.462062718017048 19.63752282867611 19.49921652293992 30.40119793036692 15-19 30.687873245835608 19.166566102524772 19.34239109679249 30.80316955484713 20-24 30.629697804493283 19.20262093899302 19.334998771685015 30.832682484828684 25-29 30.746493273783155 19.348774865144392 19.2806940252395 30.62403783583295 30-34 30.90718188264275 19.126749867909705 19.056453552647024 30.909614696800524 35-39 30.735905109843344 19.184367583266322 19.19575147978145 30.883975827108884 40-44 30.871761145507577 19.23527536907474 19.147426933839974 30.74553655157771 45-49 30.99263579772388 19.00783693672877 19.003909989159123 30.995617276388227 50-54 30.80755470311897 19.094299913441155 19.138039595743876 30.960105787695998 55-59 30.88771856145087 19.203743393392873 19.127899090803975 30.780638954352273 60-64 31.017837997894564 19.016057568161386 18.954771544684796 31.01133288925925 65-69 30.811659408969515 19.10761025196306 19.129605344342444 30.951124994724978 70-74 30.918325000000003 19.19141 19.09944 30.790824999999998 75-79 31.015078342119967 19.004436865615105 18.97605292734367 31.004431864921255 80-84 30.826704843248763 19.084290148425364 19.124984296469847 30.964020711856026 85-89 30.932006496843954 19.209791327664355 19.08735673024522 30.77084544524647 90-94 31.044420680720197 19.01379277689118 18.941061580462996 31.000724961925624 95-99 30.859652995655868 19.079242504246256 19.09477619038998 30.96632830970789 100-101 31.041227988685655 19.29807605386804 19.078683404040692 30.58201255340561 >>END_MODULE >>Per base GC content warn #Base %GC 1 61.997483504280396 2 56.050588098661215 3 58.64891352100301 4 60.244606070252914 5 58.832289774120724 6 63.10190383561726 7 61.53520563061675 8 60.28116535833138 9 62.61559817192365 10-14 60.863260648383964 15-19 61.491042800682735 20-24 61.46238028932196 25-29 61.37053110961611 30-34 61.81679657944327 35-39 61.61988093695223 40-44 61.617297697085284 45-49 61.98825307411211 50-54 61.76766049081497 55-59 61.66835751580315 60-64 62.02917088715382 65-69 61.762784403694496 70-74 61.70915 75-79 62.01951020704123 80-84 61.790725555104785 85-89 61.70285194209043 90-94 62.04514564264583 95-99 61.82598130536376 100-101 61.623240542091274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13.0 1 11.0 2 7.5 3 7.0 4 5.5 5 10.5 6 17.5 7 22.5 8 29.5 9 40.5 10 56.5 11 83.5 12 107.0 13 111.0 14 139.5 15 191.5 16 231.5 17 274.5 18 315.0 19 352.0 20 419.5 21 502.5 22 581.0 23 643.5 24 752.0 25 902.5 26 1073.0 27 1272.0 28 1477.0 29 1729.5 30 2026.0 31 2345.5 32 2720.0 33 3192.5 34 3730.0 35 4338.0 36 4879.5 37 5456.5 38 6320.0 39 7332.5 40 8307.0 41 9522.5 42 11273.5 43 13374.0 44 16004.0 45 19592.5 46 24110.5 47 29725.0 48 36707.5 49 45392.5 50 55528.5 51 66741.0 52 79192.0 53 92876.5 54 107340.0 55 121416.0 56 135112.0 57 148580.0 58 160847.5 59 171651.0 60 181113.0 61 188378.0 62 193705.0 63 197300.0 64 198140.5 65 195634.0 66 191005.0 67 183967.5 68 172765.5 69 158238.0 70 142195.0 71 125297.0 72 107589.0 73 89759.5 74 72581.5 75 56900.0 76 43309.0 77 31716.5 78 22260.0 79 15309.0 80 10002.5 81 6133.5 82 3706.0 83 2197.0 84 1299.0 85 743.0 86 474.0 87 326.5 88 215.0 89 163.0 90 135.5 91 107.5 92 85.5 93 62.5 94 46.5 95 35.5 96 28.5 97 27.5 98 22.0 99 13.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17864999999999998 2 0.1188 3 0.130375 4 0.108775 5 0.1015 6 0.1647 7 0.18145 8 0.213525 9 0.20657499999999998 10-14 0.13109 15-19 0.105215 20-24 0.06635 25-29 0.08202 30-34 0.115675 35-39 0.07815 40-44 0.055135 45-49 0.049595 50-54 0.05643 55-59 0.065005 60-64 0.00167 65-69 4.1999999999999996E-4 70-74 0.0 75-79 0.013875000000000002 80-84 0.02248 85-89 0.0037549999999999997 90-94 0.001645 95-99 0.02373 100-101 0.025137500000000004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.2777916562578059 #Duplication Level Relative count 1 100.0 2 0.19138851869294668 3 0.005511187711053438 4 0.0 5 0.0010020341292824434 6 0.0010020341292824434 7 0.0010020341292824434 8 5.010170646412217E-4 9 0.0 10++ 0.0030061023878473303 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 303005 3.0617785 3.661313 10-14 >>END_MODULE