##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.644064	34.0	31.0	34.0	31.0	34.0
2	32.80028475	34.0	31.0	34.0	31.0	34.0
3	32.85391325	34.0	31.0	34.0	31.0	34.0
4	36.22449875	37.0	37.0	37.0	35.0	37.0
5	36.230709	37.0	37.0	37.0	35.0	37.0
6	36.223744	37.0	37.0	37.0	35.0	37.0
7	36.2143705	37.0	37.0	37.0	35.0	37.0
8	36.192431	37.0	37.0	37.0	35.0	37.0
9	37.9647955	39.0	39.0	39.0	37.0	39.0
10-14	38.2585201	39.4	39.2	39.4	37.0	39.4
15-19	39.42906685	41.0	40.0	41.0	37.0	41.0
20-24	39.23149095	41.0	39.4	41.0	36.2	41.0
25-29	38.877425450000004	40.6	38.6	41.0	35.0	41.0
30-34	38.25032825	40.0	37.6	41.0	34.4	41.0
35-39	37.3177198	39.0	35.4	41.0	33.0	41.0
40-44	36.33086485	37.0	35.0	40.0	32.2	41.0
45-49	35.406555250000004	35.2	35.0	39.0	31.4	41.0
50-54	34.0705444	34.8	33.8	36.2	29.8	39.4
55-59	34.14620845	35.0	35.0	35.2	31.0	38.2
60-64	33.7183504	35.0	35.0	35.0	31.0	36.4
65-69	33.368068300000004	35.0	34.0	35.0	30.4	35.0
70-74	33.11248025	35.0	34.0	35.0	29.8	35.0
75-79	32.860684750000004	35.0	33.6	35.0	29.0	35.0
80-84	32.5883635	35.0	33.0	35.0	28.6	35.0
85-89	32.27949125	35.0	33.0	35.0	27.0	35.0
90-94	31.933331950000003	35.0	33.0	35.0	26.8	35.0
95-99	31.38671025	35.0	33.0	35.0	24.4	35.0
100-101	30.2832395	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6117.0
3	1064.0
4	1470.0
5	1832.0
6	2131.0
7	2741.0
8	3160.0
9	3636.0
10	3847.0
11	4209.0
12	4224.0
13	4377.0
14	4360.0
15	4614.0
16	4968.0
17	5760.0
18	6196.0
19	7229.0
20	8470.0
21	10036.0
22	12146.0
23	14847.0
24	17855.0
25	21811.0
26	26699.0
27	33078.0
28	40579.0
29	50871.0
30	63338.0
31	81691.0
32	108611.0
33	153051.0
34	253533.0
35	614001.0
36	1434673.0
37	853351.0
38	118561.0
39	10762.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.309778095231415	24.428558405403212	13.552585048801818	23.709078450563553
2	30.498143579071417	24.84127244369474	19.12147348232927	25.53911049490457
3	29.39586949910073	19.936419172873514	21.444135857709114	29.223575470316636
4	30.63745622162258	19.8009657396187	19.91771642172799	29.64386161703073
5	31.12828236264542	21.050841553160723	20.09677098051817	27.724105103675683
6	29.35237577512279	19.43603497538228	17.499028906548773	33.712560342946155
7	32.59846138480374	19.029004796376174	19.483009674106253	28.889524144713835
8	28.37794321286287	19.83322941472539	19.85581606155988	31.933011310851857
9	30.530645725883872	18.21313766701772	19.253967085472308	32.002249521626105
10-14	30.44887257629928	19.64291076297278	19.52561425774603	30.382602402981906
15-19	30.672361562575013	19.1807334008217	19.33030297859675	30.816602058006538
20-24	30.590366953821412	19.221821326221022	19.334499854155716	30.853311865801846
25-29	30.73196427809358	19.351629017372407	19.26992937258325	30.646477331950763
30-34	30.89801924482442	19.117761641312242	19.076906986351172	30.90731212751216
35-39	30.74253154655485	19.180357376374392	19.21545107078541	30.861660006285348
40-44	30.868782596886206	19.250576436376406	19.14015376138648	30.74048720535091
45-49	30.993066885502007	19.039554857868975	18.994882199871334	30.97249605675768
50-54	30.810020008182548	19.12557454307297	19.150615409556977	30.913790039187504
55-59	30.898769360106577	19.201600027452827	19.10435960158788	30.79527101085272
60-64	31.006697013394025	19.02706805413611	18.97931295862592	30.986921973843945
65-69	30.806974999999998	19.112015	19.131385	30.949624999999997
70-74	30.909555	19.221215	19.10286	30.76637
75-79	31.014366553329324	19.013056029878015	18.964626467813268	31.007950948979396
80-84	30.834111794470058	19.109204433470563	19.10722921491263	30.94945455714675
85-89	30.92713644032987	19.209576640700725	19.083121103264688	30.78016581570472
90-94	31.05563	19.012185000000002	18.946025	30.98616
95-99	30.85062818236149	19.10196253574074	19.1096036287991	30.93780565309868
100-101	31.017422746753482	19.299999487393443	19.077203173609714	30.60537459224336
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.01885654579497
2	56.037254073975994
3	58.619444969417366
4	60.281317838653315
5	58.85238746632111
6	63.06493611806895
7	61.48798552951757
8	60.310954523714734
9	62.53289524750998
10-14	60.83147497928118
15-19	61.48896362058155
20-24	61.44367881962326
25-29	61.37844161004434
30-34	61.80533137233658
35-39	61.60419155284019
40-44	61.60926980223712
45-49	61.96556294225969
50-54	61.72381004737005
55-59	61.69404037095929
60-64	61.99361898723797
65-69	61.75659999999999
70-74	61.675925
75-79	62.022317502308724
80-84	61.783566351616805
85-89	61.70730225603459
90-94	62.04179
95-99	61.78843383546017
100-101	61.62279733899685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	18.0
2	10.0
3	9.0
4	8.5
5	5.5
6	8.0
7	16.0
8	22.5
9	25.0
10	33.0
11	45.0
12	54.5
13	84.0
14	123.5
15	158.0
16	201.0
17	240.0
18	270.0
19	307.5
20	373.0
21	449.0
22	518.5
23	614.5
24	732.0
25	883.5
26	1055.0
27	1238.0
28	1470.0
29	1764.5
30	2081.0
31	2406.0
32	2818.0
33	3276.5
34	3728.0
35	4238.5
36	4805.5
37	5559.0
38	6444.0
39	7311.5
40	8279.5
41	9530.5
42	11276.5
43	13471.5
44	16138.5
45	19607.0
46	24125.5
47	29796.5
48	37122.0
49	45597.5
50	55206.5
51	66496.0
52	79245.0
53	92932.5
54	107217.0
55	121700.5
56	135116.0
57	148488.5
58	161289.5
59	171832.5
60	181302.0
61	189090.5
62	194268.5
63	197303.5
64	197915.5
65	196067.5
66	191263.0
67	183329.5
68	172131.5
69	158266.0
70	142244.0
71	125008.5
72	107208.5
73	89046.5
74	72249.0
75	56934.0
76	43160.5
77	31713.0
78	22451.5
79	15241.0
80	9972.0
81	6228.0
82	3757.5
83	2201.5
84	1261.0
85	741.0
86	479.5
87	310.0
88	226.0
89	164.5
90	119.5
91	86.5
92	83.5
93	71.5
94	58.0
95	48.5
96	35.0
97	30.5
98	19.5
99	9.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1779
2	0.12367500000000001
3	0.12712500000000002
4	0.10765
5	0.10435
6	0.1126
7	0.127725
8	0.16225
9	0.15655
10-14	0.09079999999999999
15-19	0.076605
20-24	0.047505
25-29	0.060765000000000007
30-34	0.08482500000000001
35-39	0.05327
40-44	0.043175
45-49	0.028335
50-54	0.043395
55-59	0.046715
60-64	1.9999999999999998E-4
65-69	0.0
70-74	0.0
75-79	0.00942
80-84	0.011065
85-89	4.25E-4
90-94	0.0
95-99	0.014305
100-101	0.0207875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.22689676197329536
#Duplication Level	Relative count
1	100.0
2	0.17832365743825043
3	0.002504545750537225
4	5.00909150107445E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.00909150107445E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	308210	3.1118221	3.661474	10-14
AAAAA	315395	3.030653	3.8962188	1
>>END_MODULE