##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54177325	34.0	31.0	34.0	31.0	34.0
2	32.69015775	34.0	31.0	34.0	31.0	34.0
3	32.75029275	34.0	31.0	34.0	31.0	34.0
4	36.12984925	37.0	37.0	37.0	35.0	37.0
5	36.13321025	37.0	37.0	37.0	35.0	37.0
6	36.13998275	37.0	37.0	37.0	35.0	37.0
7	36.134589	37.0	37.0	37.0	35.0	37.0
8	36.12050225	37.0	37.0	37.0	35.0	37.0
9	37.9012495	39.0	39.0	39.0	35.0	39.0
10-14	38.1938159	39.4	38.6	39.4	35.4	39.4
15-19	39.3507929	41.0	39.8	41.0	36.4	41.0
20-24	39.1579497	41.0	39.0	41.0	35.8	41.0
25-29	38.80397995	40.4	38.6	41.0	35.0	41.0
30-34	38.1647954	40.0	37.4	41.0	34.0	41.0
35-39	37.24642235	39.0	35.2	41.0	33.0	41.0
40-44	36.2561603	37.0	35.0	40.0	32.0	41.0
45-49	35.30157225	35.2	35.0	39.0	31.0	41.0
50-54	33.96414725	34.8	33.8	36.2	29.4	39.2
55-59	34.06237335	35.0	35.0	35.0	31.0	38.2
60-64	33.63986655	35.0	34.6	35.0	30.8	36.4
65-69	33.3166376	35.0	34.0	35.0	30.0	35.0
70-74	33.0579211	35.0	34.0	35.0	29.4	35.0
75-79	32.8054747	35.0	33.4	35.0	29.0	35.0
80-84	32.52842869999999	35.0	33.0	35.0	28.0	35.0
85-89	32.260373300000005	35.0	33.0	35.0	27.0	35.0
90-94	31.90659985	35.0	33.0	35.0	26.6	35.0
95-99	31.35802235	35.0	33.0	35.0	24.2	35.0
100-101	30.314337125	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5077.0
3	1045.0
4	1444.0
5	1683.0
6	2205.0
7	2698.0
8	3361.0
9	3882.0
10	4174.0
11	4479.0
12	4477.0
13	4567.0
14	4698.0
15	4869.0
16	5140.0
17	5812.0
18	6454.0
19	7417.0
20	8593.0
21	10498.0
22	12632.0
23	15456.0
24	18762.0
25	23223.0
26	28409.0
27	34901.0
28	43328.0
29	53648.0
30	68105.0
31	86537.0
32	115095.0
33	160588.0
34	259622.0
35	608578.0
36	1405917.0
37	844286.0
38	117714.0
39	10534.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.30855459755639	24.440858735516287	13.550571152804586	23.70001551412274
2	30.437307456525915	24.88313674285023	19.100963991912078	25.578591808711774
3	29.340076347350386	19.972997885919824	21.439803821375254	29.247121945354532
4	30.606714621088926	19.860605532293086	19.892994305788097	29.639685540829895
5	31.124770299740835	21.077959672964454	20.136861896751064	27.66040813054365
6	29.32457478111544	19.448780788109538	17.50533416150685	33.72131026926817
7	32.57431794493899	18.996618885183267	19.502709797823954	28.926353372053786
8	28.30757953702248	19.855641217957125	19.92138174265964	31.915397502360747
9	30.56742867038576	18.20888418190791	19.238249870619196	31.985437277087136
10-14	30.432207823810913	19.653067650742475	19.539930684972433	30.37479384047418
15-19	30.68265355687249	19.179531442503023	19.346850182577054	30.790964818047435
20-24	30.582599551892276	19.230045451383543	19.348385977547807	30.838969019176375
25-29	30.729654848152084	19.37388015444011	19.274198303223162	30.622266694184653
30-34	30.884528607833218	19.123339866885217	19.08145136892452	30.910680156357046
35-39	30.723039181366797	19.177354124551275	19.21772589085931	30.88188080322262
40-44	30.845391930665688	19.267898378752466	19.147787448657827	30.738922241924016
45-49	30.98182341477152	19.03046711617942	19.00175587585384	30.985953593195227
50-54	30.806075667119398	19.11643888565366	19.151566355114767	30.925919092112174
55-59	30.88218461916491	19.227931508433684	19.13126893492105	30.75861493748035
60-64	31.003805	19.03158	18.97296	30.991655
65-69	30.79345	19.115455	19.138175	30.95292
70-74	30.91181	19.21178	19.107315	30.769095
75-79	30.997660296958387	19.031734741255164	18.97034466144806	31.00026030033839
80-84	30.811337339146093	19.109397929551715	19.14283387077346	30.936430860528723
85-89	30.907235	19.220695	19.105195	30.766875
90-94	31.057015	19.001595	18.95126	30.99013
95-99	30.860447996382373	19.090934136552733	19.111879294688674	30.93673857237622
100-101	31.007735271271997	19.335915673768103	19.076414960141143	30.57993409481876
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.00857011167913
2	56.01589926523769
3	58.587198292704926
4	60.24640016191882
5	58.78517843028448
6	63.04588505038361
7	61.500671316992786
8	60.22297703938323
9	62.55286594747289
10-14	60.80700166428509
15-19	61.47361837491992
20-24	61.421568571068654
25-29	61.351921542336726
30-34	61.79520876419027
35-39	61.604919984589415
40-44	61.58431417258971
45-49	61.96777700796674
50-54	61.731994759231576
55-59	61.64079955664526
60-64	61.99546
65-69	61.74636999999999
70-74	61.680904999999996
75-79	61.997920597296776
80-84	61.74776819967482
85-89	61.674110000000006
90-94	62.047145
95-99	61.79718656875859
100-101	61.58766936609076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	10.0
2	6.5
3	6.5
4	9.0
5	9.0
6	7.5
7	10.5
8	15.0
9	18.5
10	23.5
11	31.0
12	44.0
13	60.5
14	79.0
15	88.0
16	108.5
17	141.0
18	181.5
19	227.5
20	279.5
21	363.5
22	453.0
23	537.0
24	643.5
25	754.0
26	919.0
27	1214.0
28	1460.5
29	1681.5
30	2033.0
31	2396.5
32	2727.0
33	3262.0
34	3805.0
35	4219.5
36	4874.0
37	5577.0
38	6293.0
39	7294.0
40	8420.5
41	9762.5
42	11470.5
43	13628.5
44	16346.0
45	19812.5
46	24344.5
47	30120.5
48	36987.0
49	45450.0
50	55804.5
51	67252.5
52	79623.5
53	93011.0
54	107267.5
55	121909.5
56	135939.0
57	148773.0
58	161068.5
59	172156.0
60	181310.0
61	188997.0
62	194339.0
63	196869.0
64	197703.0
65	195900.0
66	190937.0
67	183231.0
68	172001.5
69	158039.0
70	142220.5
71	125067.5
72	106909.0
73	88911.5
74	72189.5
75	56619.0
76	42927.0
77	31541.5
78	22375.5
79	15232.0
80	9858.5
81	6182.5
82	3708.0
83	2147.5
84	1251.5
85	740.0
86	476.5
87	298.5
88	199.5
89	167.5
90	145.0
91	121.5
92	90.5
93	66.5
94	48.5
95	43.0
96	39.5
97	29.0
98	22.0
99	16.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25217500000000004
2	0.209225
3	0.193
4	0.1969
5	0.209625
6	0.10145
7	0.1217
8	0.1377
9	0.1011
10-14	0.05919
15-19	0.038095000000000004
20-24	0.017345
25-29	0.016905
30-34	0.04416
35-39	0.01676
40-44	0.0091
45-49	0.00432
50-54	0.00703
55-59	0.007835
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.3000000000000002E-4
80-84	0.002815
85-89	0.0
90-94	0.0
95-99	7.549999999999999E-4
100-101	2.75E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.24737878439564956
#Duplication Level	Relative count
1	100.0
2	0.19338386697594726
3	0.004007955792247612
4	5.009944740309515E-4
5	0.0
6	0.0
7	0.0
8	5.009944740309515E-4
9	0.0
10++	5.009944740309515E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	308050	3.1024559	3.7643092	10-14
AAAAA	314745	3.0202682	3.959673	1
>>END_MODULE