##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	767247	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49103222299989	34.0	31.0	34.0	31.0	34.0
2	32.75415348642615	34.0	31.0	34.0	31.0	34.0
3	32.79759842658231	34.0	31.0	34.0	31.0	34.0
4	36.28372610124249	37.0	37.0	37.0	35.0	37.0
5	36.17752301410106	37.0	37.0	37.0	35.0	37.0
6	36.173605110218745	37.0	37.0	37.0	35.0	37.0
7	36.13333515803907	37.0	37.0	37.0	35.0	37.0
8	36.13620776620827	37.0	37.0	37.0	35.0	37.0
9	37.87530612697085	39.0	38.0	39.0	35.0	39.0
10-14	38.125834835457155	39.4	38.2	39.4	35.2	39.4
15-19	39.21074725609875	40.6	39.0	41.0	36.0	41.0
20-24	38.97525907563014	40.0	38.4	41.0	35.2	41.0
25-29	38.52189633846727	40.0	38.0	41.0	34.4	41.0
30-34	37.848650564941934	39.8	36.8	41.0	33.0	41.0
35-39	36.82741411826961	38.6	35.0	40.4	31.8	41.0
40-44	35.78054524814043	36.6	35.0	40.0	30.6	41.0
45-49	34.75569901218252	35.0	34.6	38.6	28.4	40.8
50-54	33.12640205826807	35.0	32.8	36.0	24.4	39.4
55-59	33.15490787191087	35.0	33.0	35.0	27.2	37.6
60-64	33.19334634087849	35.0	33.0	35.0	29.0	36.2
65-69	32.81099984750673	35.0	33.0	35.0	29.0	35.0
70-74	32.45390936686621	35.0	33.0	35.0	27.0	35.0
75-79	31.962948046717678	34.4	32.4	35.0	26.4	35.0
80-84	32.22742923725997	35.0	33.0	35.0	27.0	35.0
85-89	31.96713105427587	35.0	33.0	35.0	27.0	35.0
90-94	31.544974695241557	35.0	32.8	35.0	25.0	35.0
95-99	30.977458106711403	34.6	32.0	35.0	23.6	35.0
100-101	29.600319714511755	33.5	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	2.0
4	5.0
5	28.0
6	52.0
7	211.0
8	433.0
9	683.0
10	882.0
11	1004.0
12	1129.0
13	1095.0
14	1151.0
15	1210.0
16	1340.0
17	1480.0
18	1600.0
19	1711.0
20	1916.0
21	2099.0
22	2577.0
23	3203.0
24	4272.0
25	5508.0
26	6896.0
27	8807.0
28	10945.0
29	13644.0
30	17017.0
31	21390.0
32	28144.0
33	39408.0
34	65291.0
35	146544.0
36	250874.0
37	110610.0
38	13263.0
39	813.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.3764886228544	24.795216593321793	13.872739691742966	22.95555509208084
2	30.056422508005898	24.75734672145997	20.030837526898118	25.155393243636016
3	28.802328324516097	20.12689525016064	22.365548513060332	28.705227912262938
4	30.225859468984563	20.46225009677457	20.724356041796188	28.587534392444674
5	30.91038479133838	21.08564777705224	20.375576574427793	27.628390857181586
6	29.313506602176354	19.563321850720826	17.81017064908693	33.31300089801589
7	32.5365886083621	19.072345672254176	19.71933419094503	28.67173152843869
8	28.416533397980047	19.842371491840307	19.981700808214303	31.759394301965337
9	30.55782557637892	18.264392040633588	19.432073374024274	31.745709008963214
10-14	30.376658364255576	19.632035055203865	19.660031254602494	30.331275325938062
15-19	30.657011366613357	19.172157075882996	19.39680441891594	30.774027138587705
20-24	30.580670725587495	19.2541439704692	19.39363841715122	30.771546886792084
25-29	30.73349415278613	19.381098830974306	19.300445807372217	30.58496120886735
30-34	30.91009212985933	19.090273071463116	19.137618814337387	30.862015984340168
35-39	30.75418896810757	19.20215992160532	19.261821654184118	30.781829456102994
40-44	31.041813928033292	19.279060496164448	19.136046949118203	30.54307862668406
45-49	31.644620311320864	19.057487354137585	18.874677906853986	30.42321442768756
50-54	31.359557315846736	19.184700411914392	19.00757357345772	30.448168698781146
55-59	30.94265601559863	19.289642057903126	19.151902842239853	30.615799084258395
60-64	30.975537971969437	19.045668865208718	19.00101328796695	30.977779874854892
65-69	30.827381273580468	19.147918949277997	19.138586474115286	30.886113303026253
70-74	30.914373076727575	19.25700589249616	19.137279129146158	30.691341901630114
75-79	30.969036047061767	19.038458280058443	19.014059357677514	30.978446315202273
80-84	30.84059698255525	19.09556423422395	19.155492924556583	30.908345858664216
85-89	30.875332422370484	19.244159767104925	19.137883664030923	30.742624146493668
90-94	31.049150779823727	19.014288838876404	18.998047670826196	30.938512710473674
95-99	30.841254511259088	19.095154493924383	19.157246623316873	30.90634437149966
100-101	31.055188224913227	19.381177117668756	19.08883319191864	30.474801465499375
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.33204371493524
2	55.211815751641915
3	57.50755623677903
4	58.81339386142924
5	58.53877564851997
6	62.62650750019224
7	61.2083201368008
8	60.17592769994538
9	62.303534585342135
10-14	60.70793369019364
15-19	61.431038505201066
20-24	61.352217612379576
25-29	61.31845536165348
30-34	61.7721081141995
35-39	61.53601842421056
40-44	61.58489255471735
45-49	62.06783473900843
50-54	61.80772601462788
55-59	61.55845509985702
60-64	61.953317846824326
65-69	61.71349457660672
70-74	61.60571497835768
75-79	61.94748236226404
80-84	61.74894284121947
85-89	61.61795656886415
90-94	61.98766349029739
95-99	61.747598882758744
100-101	61.5299896904126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	1.0
5	1.0
6	1.0
7	1.5
8	1.5
9	1.0
10	3.5
11	4.5
12	4.5
13	8.0
14	10.5
15	15.5
16	20.0
17	23.0
18	29.0
19	36.0
20	46.0
21	65.0
22	85.0
23	105.0
24	119.5
25	143.0
26	172.0
27	209.5
28	262.5
29	327.0
30	387.5
31	455.5
32	552.5
33	620.5
34	728.5
35	864.0
36	986.0
37	1103.5
38	1240.0
39	1393.5
40	1580.5
41	1848.5
42	2212.0
43	2680.5
44	3203.5
45	3865.0
46	4691.5
47	5748.5
48	7134.5
49	8861.0
50	10837.5
51	13003.0
52	15468.5
53	18177.0
54	20945.0
55	23602.5
56	26052.5
57	28559.0
58	30886.5
59	32924.5
60	34769.5
61	36273.5
62	37522.0
63	38056.0
64	38007.5
65	37688.5
66	36791.5
67	35229.5
68	32885.0
69	30256.5
70	27238.0
71	23767.5
72	20366.5
73	16934.0
74	13617.0
75	10628.0
76	8090.0
77	5860.5
78	4031.5
79	2708.0
80	1730.5
81	1065.5
82	618.5
83	333.0
84	179.0
85	95.0
86	61.0
87	47.5
88	29.0
89	17.5
90	13.0
91	6.5
92	5.0
93	6.0
94	3.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01238193176382573
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.006256133943827737
25-29	0.0012772940135314965
30-34	0.07124172528533836
35-39	0.033392114925180544
40-44	0.024346787931396278
45-49	0.0
50-54	2.6067224765948908E-5
55-59	0.0
60-64	0.005421982751317373
65-69	0.004379293760679416
70-74	0.0
75-79	0.0
80-84	2.3460502289354015E-4
85-89	2.6067224765948908E-5
90-94	0.007924436328848468
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	767247.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.04299441072660864
#Duplication Level	Relative count
1	100.0
2	0.029008702610783234
3	5.001500450135041E-4
4	0.0
5	0.0
6	0.0
7	5.001500450135041E-4
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	59135	3.0700307	3.876828	9
AAAAA	60750	3.0102098	3.7487261	1
>>END_MODULE