##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.704876	34.0	31.0	34.0	31.0	34.0
2	32.956395	34.0	31.0	34.0	31.0	34.0
3	33.01766875	34.0	31.0	34.0	31.0	34.0
4	36.4263835	37.0	37.0	37.0	35.0	37.0
5	36.3554235	37.0	37.0	37.0	35.0	37.0
6	36.340436	37.0	37.0	37.0	35.0	37.0
7	36.34102825	37.0	37.0	37.0	35.0	37.0
8	36.32245725	37.0	37.0	37.0	35.0	37.0
9	38.1153665	39.0	39.0	39.0	37.0	39.0
10-14	38.3753327	39.4	38.6	39.4	37.0	39.4
15-19	39.53795885	41.0	40.0	41.0	37.0	41.0
20-24	39.320697949999996	41.0	39.0	41.0	36.2	41.0
25-29	38.78500955	40.0	38.4	41.0	35.0	41.0
30-34	37.7185403	40.0	37.0	41.0	32.8	41.0
35-39	37.1931653	38.8	35.2	40.6	32.8	41.0
40-44	36.3479342	37.2	35.0	40.0	32.4	41.0
45-49	35.671858199999996	35.2	35.0	39.2	32.2	41.0
50-54	34.73617505	35.0	35.0	36.6	31.2	40.0
55-59	33.82396285	35.0	34.0	35.0	30.0	38.0
60-64	33.435719649999996	35.0	34.0	35.0	30.0	36.2
65-69	33.03950325	35.0	33.2	35.0	29.4	35.0
70-74	32.71785145	35.0	33.0	35.0	29.0	35.0
75-79	32.26293439999999	34.6	32.6	35.0	27.8	35.0
80-84	32.451310899999996	35.0	33.0	35.0	28.2	35.0
85-89	31.566938400000005	35.0	32.2	35.0	23.6	35.0
90-94	31.489597049999997	35.0	32.8	35.0	24.4	35.0
95-99	31.168758249999996	35.0	33.0	35.0	24.0	35.0
100-101	29.82793575	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24.0
3	14.0
4	26.0
5	91.0
6	270.0
7	805.0
8	1934.0
9	3062.0
10	3937.0
11	4442.0
12	4770.0
13	5111.0
14	5451.0
15	5992.0
16	6638.0
17	7168.0
18	7920.0
19	8595.0
20	9330.0
21	10663.0
22	12534.0
23	15284.0
24	19249.0
25	23749.0
26	30062.0
27	37242.0
28	46610.0
29	58470.0
30	73025.0
31	93038.0
32	123794.0
33	175035.0
34	295684.0
35	708596.0
36	1389358.0
37	711925.0
38	93509.0
39	6560.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.592672760241314	24.67762730093444	13.716368025508313	23.013331913315938
2	30.122	24.659775	19.934350000000002	25.283875000000002
3	28.962775	20.098	22.170925	28.768300000000004
4	30.322850000000003	20.238725	20.57045	28.867975
5	31.036825	21.088575000000002	20.28105	27.59355
6	29.312525	19.525125	17.804425	33.357925
7	32.485725	19.172725	19.612975	28.728575
8	28.376625	19.8297	19.994375	31.799300000000002
9	30.504649999999998	18.213075	19.3918	31.890475000000002
10-14	30.432385	19.64692	19.591065	30.32963
15-19	30.68764	19.17159	19.378545	30.762224999999997
20-24	30.590073370392723	19.232153563286904	19.371088775024965	30.80668429129541
25-29	30.834590177475523	19.35827648117774	19.28148082073506	30.52565252061168
30-34	31.309699478928138	19.086578956639265	19.012397475027303	30.591324089405298
35-39	30.727949575908802	19.177356954592	19.248860339774122	30.845833129725076
40-44	30.90403298880231	19.253838235867015	19.181295036631916	30.660833738698756
45-49	30.963214999999998	19.056695	19.036434999999997	30.943654999999996
50-54	30.83727004422553	19.10574241873257	19.177312465253102	30.8796750717888
55-59	30.988495	19.227925000000003	19.125625	30.657954999999998
60-64	31.027671364157218	19.01542027626457	18.995147152176152	30.961761207402063
65-69	30.844746391368837	19.104062297438045	19.17351746837552	30.8776738428176
70-74	30.96583	19.246845	19.09186	30.695465
75-79	31.043235000000003	19.008895	18.999645	30.948225
80-84	30.844488653446067	19.088943676806373	19.14010380726471	30.92646386248285
85-89	30.9631269807547	19.200823494946434	19.098466637169466	30.7375828871294
90-94	31.057996088874734	18.99427593125757	18.991300531661402	30.95642744820629
95-99	30.902159635323944	19.07041786056268	19.11326786699018	30.914154637123197
100-101	31.182212500000002	19.2524625	19.0744	30.490925
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.606004673557244
2	55.405875
3	57.731075
4	59.190825000000004
5	58.630375
6	62.67045
7	61.2143
8	60.175925
9	62.39512499999999
10-14	60.762015000000005
15-19	61.449865
20-24	61.39675766168814
25-29	61.360242698087205
30-34	61.901023568333436
35-39	61.573782705633874
40-44	61.56486672750107
45-49	61.906870000000005
50-54	61.71694511601432
55-59	61.646449999999994
60-64	61.98943257155928
65-69	61.72242023418644
70-74	61.661295
75-79	61.99146
80-84	61.77095251592891
85-89	61.70070986788411
90-94	62.01442353708103
95-99	61.81631427244714
100-101	61.673137499999996
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	4.5
3	2.0
4	4.0
5	3.5
6	4.0
7	7.0
8	10.0
9	17.5
10	24.5
11	28.0
12	29.5
13	46.0
14	67.0
15	91.0
16	107.0
17	133.0
18	172.5
19	201.5
20	262.5
21	347.0
22	397.5
23	474.5
24	625.0
25	772.0
26	937.0
27	1143.5
28	1375.5
29	1659.5
30	1988.0
31	2343.0
32	2747.5
33	3224.5
34	3777.0
35	4325.0
36	4962.5
37	5700.5
38	6524.0
39	7429.5
40	8432.5
41	9803.5
42	11478.5
43	13645.0
44	16409.0
45	19980.5
46	24701.0
47	30433.5
48	37511.5
49	45983.5
50	55988.5
51	67488.5
52	80486.5
53	94231.0
54	108339.5
55	122674.0
56	136907.5
57	149799.0
58	161213.0
59	171979.5
60	180920.5
61	188315.0
62	194020.0
63	197121.5
64	197765.0
65	195607.0
66	190236.5
67	182120.5
68	171040.0
69	157172.0
70	141533.0
71	124303.0
72	106408.5
73	88823.0
74	71818.0
75	56339.0
76	42827.5
77	31360.0
78	22241.0
79	15303.0
80	9963.5
81	6197.5
82	3705.5
83	2133.0
84	1237.5
85	702.5
86	420.5
87	274.5
88	194.0
89	136.0
90	104.5
91	67.5
92	44.0
93	37.5
94	23.0
95	13.0
96	7.5
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001225
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00735
25-29	8.600000000000001E-4
30-34	0.08288
35-39	0.032705
40-44	0.018195
45-49	0.0
50-54	6.500000000000001E-5
55-59	0.0
60-64	0.01541
65-69	0.007444999999999999
70-74	0.0
75-79	0.0
80-84	2.55E-4
85-89	0.001815
90-94	0.013430000000000001
95-99	1.4999999999999999E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.16598589119925164
#Duplication Level	Relative count
1	100.0
2	0.1547437213611438
3	0.002003154969076295
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010015774845381475
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	313105	3.11211	3.5409234	90-94
AAAAA	317760	3.0441012	3.6976504	1
>>END_MODULE