##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.771935	34.0	31.0	34.0	31.0	34.0
2	33.0208455	34.0	31.0	34.0	31.0	34.0
3	33.075834	34.0	33.0	34.0	31.0	34.0
4	36.47037475	37.0	37.0	37.0	35.0	37.0
5	36.4012355	37.0	37.0	37.0	35.0	37.0
6	36.388276	37.0	37.0	37.0	35.0	37.0
7	36.388355	37.0	37.0	37.0	35.0	37.0
8	36.376217	37.0	37.0	37.0	35.0	37.0
9	38.17603825	39.0	39.0	39.0	37.0	39.0
10-14	38.43039645	39.4	38.8	39.4	37.0	39.4
15-19	39.59310865	41.0	40.0	41.0	37.0	41.0
20-24	39.18122089999999	40.8	39.0	41.0	36.0	41.0
25-29	37.287748799999996	40.0	36.4	41.0	28.8	41.0
30-34	34.10818055000001	38.4	28.8	40.2	21.0	41.0
35-39	36.733522799999996	38.6	35.0	40.0	31.8	41.0
40-44	27.239887699999997	27.0	23.6	31.0	17.6	39.2
45-49	32.521073	33.2	30.8	36.4	26.6	38.6
50-54	32.777349150000006	35.0	32.2	36.0	22.8	39.6
55-59	30.106373	34.0	27.2	35.0	16.4	37.0
60-64	32.767407649999996	35.0	33.0	35.0	27.0	35.8
65-69	32.76257985	35.0	33.0	35.0	28.6	35.0
70-74	31.636571100000005	35.0	32.0	35.0	22.6	35.0
75-79	31.502517949999998	34.4	32.0	35.0	24.0	35.0
80-84	31.6830872	35.0	32.4	35.0	24.8	35.0
85-89	30.923790450000002	34.6	31.0	35.0	21.0	35.0
90-94	31.231773549999996	34.8	32.4	35.0	23.8	35.0
95-99	31.0597699	35.0	32.6	35.0	23.6	35.0
100-101	29.784257125	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	9.0
4	33.0
5	109.0
6	351.0
7	1181.0
8	2966.0
9	4927.0
10	6325.0
11	7811.0
12	8677.0
13	7336.0
14	6367.0
15	6600.0
16	7010.0
17	7194.0
18	7865.0
19	8788.0
20	9815.0
21	11415.0
22	14305.0
23	18351.0
24	24118.0
25	31107.0
26	40339.0
27	50889.0
28	64997.0
29	84637.0
30	113756.0
31	164041.0
32	274693.0
33	530034.0
34	898450.0
35	880178.0
36	506851.0
37	174523.0
38	22372.0
39	1545.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.49216709529826	24.685878564719367	13.741568661579104	23.08038567840327
2	30.15005	24.63615	19.905025000000002	25.308775
3	28.948974999999997	20.05735	22.197975	28.795700000000004
4	30.307050000000004	20.25945	20.576775	28.856725
5	31.039325	21.0967	20.3276	27.536375000000003
6	29.304049999999997	19.5432	17.769575	33.383175
7	32.440675	19.1826	19.626825	28.7499
8	28.3508	19.848	20.030524999999997	31.770674999999997
9	30.50985	18.221024999999997	19.384725	31.884400000000003
10-14	30.425780000000003	19.657645000000002	19.59173	30.324845
15-19	30.679699999999997	19.17562	19.3877	30.75698
20-24	30.9202797860154	19.201932841556093	19.27357438184921	30.6042129905793
25-29	31.012546011659502	19.23581619876417	19.169010604194376	30.58262718538195
30-34	31.557564756951713	19.09670613338556	18.902877421364387	30.44285168829834
35-39	30.890592871594375	19.23420196044082	19.179007083317558	30.696198084647246
40-44	38.19487213501176	17.499894769200157	17.036741493205916	27.268491602582174
45-49	31.06076	19.08381	19.070705	30.784725
50-54	31.198554474001067	19.140484433010396	19.07840417537733	30.58255691761121
55-59	31.118588254615492	19.569279495525834	19.037709132752372	30.274423117106302
60-64	31.20175213744026	19.081818053620452	18.943955944932767	30.77247386400652
65-69	30.873846587419884	19.133531512788565	19.15317811103933	30.839443788752213
70-74	30.99846	19.32222	19.052130000000002	30.62719
75-79	31.16992	19.1803	18.92416	30.72562
80-84	30.91710514019661	19.126167625930897	19.11876258038987	30.83796465348262
85-89	31.054163905624044	19.28912211545635	19.104793143320265	30.55192083559934
90-94	31.091509525768384	18.978930962820364	18.978415886125443	30.951143625285805
95-99	30.903768892060455	19.05875598801916	19.106036139315645	30.931438980604735
100-101	31.1329625	19.2113	19.0900875	30.565649999999998
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.57255277370153
2	55.458825
3	57.744675
4	59.163774999999994
5	58.5757
6	62.687225000000005
7	61.190575
8	60.121475000000004
9	62.39424999999999
10-14	60.750625
15-19	61.436679999999996
20-24	61.5244927765947
25-29	61.595173197041454
30-34	62.00041644525005
35-39	61.58679095624162
40-44	65.46336373759392
45-49	61.845485
50-54	61.78111139161228
55-59	61.3930113717218
60-64	61.97422600144677
65-69	61.713290376172104
70-74	61.62565
75-79	61.895540000000004
80-84	61.755069793679226
85-89	61.60608474122339
90-94	62.042653151054196
95-99	61.83520787266519
100-101	61.6986125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	2.0
3	1.5
4	2.5
5	4.0
6	4.0
7	6.5
8	12.0
9	12.0
10	14.0
11	21.0
12	30.5
13	36.0
14	42.5
15	67.0
16	88.5
17	113.0
18	139.5
19	170.0
20	220.0
21	287.0
22	365.5
23	439.0
24	548.0
25	662.0
26	815.0
27	1001.0
28	1229.5
29	1513.0
30	1819.5
31	2140.5
32	2538.5
33	2970.5
34	3463.0
35	4102.0
36	4778.0
37	5434.5
38	6143.5
39	7027.5
40	8191.5
41	9589.0
42	11128.0
43	13266.0
44	16003.5
45	19385.0
46	23860.5
47	29442.5
48	36463.0
49	45361.0
50	55460.0
51	66892.0
52	79627.0
53	93596.5
54	108318.0
55	122907.5
56	137623.5
57	151263.0
58	163694.5
59	174921.5
60	184822.5
61	193004.5
62	198506.5
63	201542.5
64	201901.5
65	198887.5
66	192850.0
67	184244.5
68	171936.5
69	157157.0
70	140591.5
71	122242.0
72	103681.5
73	85570.5
74	68576.5
75	53461.0
76	40247.0
77	28914.0
78	20203.5
79	13649.0
80	8750.0
81	5401.5
82	3276.0
83	1996.0
84	1175.0
85	716.5
86	469.5
87	303.5
88	205.0
89	153.5
90	105.5
91	72.5
92	50.5
93	34.5
94	18.5
95	12.5
96	5.5
97	3.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030699999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00215
25-29	8.9E-4
30-34	3.396665
35-39	0.017895
40-44	2.001125
45-49	0.0
50-54	4.15E-4
55-59	1.7242049999999998
60-64	0.012410000000000001
65-69	0.008135
70-74	0.0
75-79	0.0
80-84	6.150000000000001E-4
85-89	0.00758
90-94	0.014889999999999999
95-99	3.1999999999999997E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.017499300028011203
#Duplication Level	Relative count
1	100.0
2	0.012001620218729529
3	0.0010001350182274606
4	0.0
5	0.0
6	5.000675091137303E-4
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	289645	3.0458624	3.6939929	10-14
>>END_MODULE