##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73461425	34.0	31.0	34.0	31.0	34.0
2	32.98204425	34.0	31.0	34.0	31.0	34.0
3	32.93005975	34.0	31.0	34.0	31.0	34.0
4	36.40267075	37.0	37.0	37.0	35.0	37.0
5	36.36162825	37.0	37.0	37.0	35.0	37.0
6	36.36165325	37.0	37.0	37.0	35.0	37.0
7	36.363512	37.0	37.0	37.0	35.0	37.0
8	36.2936635	37.0	37.0	37.0	35.0	37.0
9	38.13885225	39.0	39.0	39.0	37.0	39.0
10-14	38.097752150000005	39.4	38.4	39.4	36.0	39.4
15-19	39.2322708	41.0	39.0	41.0	36.2	41.0
20-24	39.18504615	40.8	39.0	41.0	35.8	41.0
25-29	37.02555195	39.6	36.4	41.0	28.6	41.0
30-34	34.57397364999999	38.2	33.2	40.2	20.8	41.0
35-39	35.43270625	37.8	34.2	40.0	27.0	41.0
40-44	29.159393799999997	28.2	23.4	37.6	16.2	40.6
45-49	32.10944005	34.0	30.4	36.6	22.2	40.0
50-54	33.02742505	35.0	32.6	36.2	24.2	39.4
55-59	31.579755849999998	35.0	31.6	35.0	20.2	37.2
60-64	33.03035165000001	35.0	33.0	35.0	28.2	35.8
65-69	31.771378250000005	35.0	32.4	35.0	23.6	35.0
70-74	31.05528195	35.0	31.0	35.0	20.6	35.0
75-79	31.550352200000003	34.4	32.4	35.0	25.4	35.0
80-84	32.09141715	35.0	33.0	35.0	26.4	35.0
85-89	31.9640288	35.0	33.0	35.0	27.0	35.0
90-94	31.575910699999998	35.0	33.0	35.0	25.0	35.0
95-99	30.352833049999997	34.8	32.0	35.0	14.4	35.0
100-101	27.948448375	33.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39.0
3	8.0
4	35.0
5	129.0
6	383.0
7	1251.0
8	3338.0
9	6113.0
10	7276.0
11	8212.0
12	8377.0
13	7577.0
14	7270.0
15	7429.0
16	7819.0
17	7692.0
18	8784.0
19	9361.0
20	10378.0
21	11913.0
22	14629.0
23	19352.0
24	25248.0
25	33124.0
26	43068.0
27	57552.0
28	77331.0
29	103420.0
30	137145.0
31	191573.0
32	297880.0
33	466326.0
34	651952.0
35	717360.0
36	704472.0
37	305848.0
38	37808.0
39	2519.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.480340185996084	24.689739809523047	13.767420879650652	23.062499124830214
2	30.13735	24.652575	19.939899999999998	25.270175000000002
3	29.446424999999998	19.94535	22.058825000000002	28.549400000000002
4	30.319325000000003	20.282275	20.5678	28.8306
5	31.004575000000003	21.124950000000002	20.2924	27.578075000000002
6	29.3169	19.507525	17.791275000000002	33.3843
7	32.474875	19.18385	19.607925	28.73335
8	28.438950000000002	19.85055	19.998075	31.712425
9	30.456925000000002	18.236475	19.40115	31.905450000000002
10-14	30.580144999999998	19.700895	19.519845	30.199115
15-19	30.711105	19.155829999999998	19.338235	30.79483
20-24	30.792947383179232	19.201877516423817	19.34148022471636	30.663694875680587
25-29	31.49899802384912	19.242012304728156	19.101100111160672	30.157889560262053
30-34	32.23660020970189	19.07542248697318	18.60168953263155	30.086287770693374
35-39	31.653519980224587	19.18891796668695	18.980196931000137	30.17736512208833
40-44	36.18767417503117	18.36341898622994	17.639612208120017	27.80929463061887
45-49	31.40263	19.15097	19.01173	30.434670000000004
50-54	31.35079948587943	19.341861276047403	19.076560984217082	30.230778253856077
55-59	31.276891255378253	19.50671890134378	19.01242880248576	30.20396104079221
60-64	31.057868040613272	19.04838610866964	18.987523707955496	30.906222142761585
65-69	31.04312346443951	19.351755308482318	19.054407286776815	30.55071394030136
70-74	31.243205183058738	19.409254746302917	19.00585706157702	30.34168300906132
75-79	31.174725	19.06139	18.946170000000002	30.817715
80-84	30.840436431128992	19.099987049669767	19.131752859697922	30.927823659503318
85-89	30.957612682694446	19.16982666678202	19.10390036371736	30.76866028680617
90-94	31.082441107118207	18.979012834883974	18.954786353088767	30.983759704909048
95-99	30.89505778077696	19.09145692119796	19.148123173522137	30.865362124502955
100-101	31.429875000000003	19.491925	18.9502375	30.1279625
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.5428393108263
2	55.407525
3	57.995825
4	59.149925
5	58.58265
6	62.7012
7	61.208225000000006
8	60.151374999999994
9	62.362375
10-14	60.77926
15-19	61.505935
20-24	61.456642258859816
25-29	61.65688758411116
30-34	62.32288798039527
35-39	61.83088510231292
40-44	63.99696880565004
45-49	61.83729999999999
50-54	61.581577739735515
55-59	61.48085229617046
60-64	61.964090183374864
65-69	61.59383740474087
70-74	61.584888192120054
75-79	61.99244
80-84	61.76826009063231
85-89	61.72627296950062
90-94	62.06620081202726
95-99	61.7604199052799
100-101	61.5578375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	5.0
2	3.5
3	4.0
4	3.5
5	3.0
6	5.0
7	8.0
8	10.0
9	8.0
10	9.5
11	16.0
12	23.5
13	24.5
14	40.5
15	64.5
16	79.0
17	102.0
18	135.5
19	180.5
20	230.0
21	277.0
22	336.5
23	432.5
24	522.0
25	621.5
26	808.0
27	1001.0
28	1195.5
29	1473.5
30	1788.0
31	2106.5
32	2469.5
33	2952.5
34	3491.0
35	4005.0
36	4585.0
37	5294.5
38	6117.5
39	7078.0
40	8149.0
41	9448.0
42	11102.5
43	13059.5
44	15670.0
45	19049.5
46	23286.0
47	28983.5
48	36086.0
49	44249.0
50	53989.5
51	65251.5
52	77945.5
53	91806.0
54	106350.5
55	121090.5
56	135624.5
57	149687.5
58	162433.5
59	173673.0
60	183291.0
61	191508.5
62	197613.5
63	200651.5
64	201547.0
65	199085.0
66	193423.5
67	185091.0
68	173212.5
69	159087.5
70	142564.5
71	124588.0
72	106294.5
73	88151.5
74	70784.5
75	54985.5
76	41667.5
77	30533.5
78	21555.0
79	14725.5
80	9688.0
81	6050.0
82	3623.0
83	2153.5
84	1303.0
85	789.0
86	500.5
87	345.0
88	238.5
89	165.5
90	117.5
91	83.5
92	54.0
93	37.5
94	22.0
95	9.0
96	5.0
97	3.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0194
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00194
25-29	0.005105
30-34	0.06289499999999999
35-39	0.017265
40-44	1.490975
45-49	0.0
50-54	1.1E-4
55-59	1.9999999999999998E-5
60-64	0.020375
65-69	0.01615
70-74	0.0019049999999999998
75-79	0.0
80-84	0.00255
85-89	0.017145
90-94	0.026755
95-99	0.0022099999999999997
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.04649814007440582
#Duplication Level	Relative count
1	100.0
2	0.04051721981842283
3	0.0015006377710526974
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.002125903508991E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	299150	3.1042194	3.7629354	85-89
>>END_MODULE