##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75234525	34.0	31.0	34.0	31.0	34.0
2	32.98680875	34.0	31.0	34.0	31.0	34.0
3	33.02396175	34.0	31.0	34.0	31.0	34.0
4	36.436117	37.0	37.0	37.0	35.0	37.0
5	36.377952	37.0	37.0	37.0	35.0	37.0
6	36.360007	37.0	37.0	37.0	35.0	37.0
7	36.3661305	37.0	37.0	37.0	35.0	37.0
8	36.34666325	37.0	37.0	37.0	35.0	37.0
9	38.1537505	39.0	39.0	39.0	37.0	39.0
10-14	38.34042405	39.4	38.8	39.4	36.2	39.4
15-19	39.545869249999996	41.0	40.0	41.0	37.0	41.0
20-24	39.310251799999996	41.0	39.0	41.0	36.0	41.0
25-29	38.4412899	40.0	38.4	41.0	34.0	41.0
30-34	36.9122048	39.2	36.0	41.0	29.8	41.0
35-39	36.9816553	38.8	35.0	40.6	32.2	41.0
40-44	35.94355465	37.0	35.0	40.0	31.0	41.0
45-49	35.454700700000004	35.2	35.0	39.2	31.6	41.0
50-54	34.5061108	35.0	34.8	36.6	30.8	40.0
55-59	33.21544075	35.0	33.4	35.0	27.2	38.0
60-64	33.15863645	35.0	33.2	35.0	28.6	36.2
65-69	32.760477349999995	35.0	33.0	35.0	28.2	35.0
70-74	32.4478907	35.0	33.0	35.0	27.2	35.0
75-79	31.833621849999997	34.4	32.6	35.0	26.0	35.0
80-84	32.128803899999994	35.0	33.0	35.0	26.4	35.0
85-89	31.808425749999998	35.0	33.0	35.0	25.6	35.0
90-94	31.579906799999996	35.0	33.0	35.0	24.6	35.0
95-99	31.12605235	35.0	33.0	35.0	23.8	35.0
100-101	29.738219375	34.0	30.5	35.0	10.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39.0
3	13.0
4	39.0
5	102.0
6	313.0
7	906.0
8	2209.0
9	3687.0
10	4601.0
11	5174.0
12	5543.0
13	5719.0
14	6244.0
15	6785.0
16	7104.0
17	7921.0
18	8471.0
19	9165.0
20	10227.0
21	10905.0
22	12655.0
23	15274.0
24	19150.0
25	24277.0
26	30549.0
27	38936.0
28	48567.0
29	61653.0
30	78690.0
31	102611.0
32	140942.0
33	209899.0
34	364795.0
35	768302.0
36	1271884.0
37	627710.0
38	82942.0
39	5969.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.48286465530995	24.642320717714334	13.739435925829783	23.13537870114592
2	30.148875000000004	24.6577	19.92795	25.265475
3	29.036925000000004	20.00865	22.205025	28.7494
4	30.285375	20.2727	20.589825	28.852100000000004
5	30.993274999999997	21.108975	20.305300000000003	27.592450000000003
6	29.325975	19.5229	17.7737	33.377425
7	32.511849999999995	19.163825000000003	19.5942	28.730125
8	28.320725000000003	19.8608	20.061425	31.75705
9	30.492575	18.222450000000002	19.3359	31.949075
10-14	30.50195067568274	19.64265187492816	19.584996854748898	30.270400594640208
15-19	30.686365	19.174615	19.397100000000002	30.74192
20-24	30.62692792909596	19.226147829851783	19.375349597892736	30.77157464315952
25-29	30.993072755220584	19.327753950057556	19.312933640313084	30.366239654408776
30-34	31.61343838530809	19.12459055360574	18.879522968314536	30.382448092771636
35-39	30.882221296924108	19.186402571959793	19.204081144799364	30.727294986316732
40-44	31.153068445733478	19.256552507690472	19.098060367619563	30.492318678956487
45-49	31.048605000000002	19.074099999999998	19.025624999999998	30.85167
50-54	30.919069999999998	19.15774	19.1535	30.76969
55-59	31.169964999999998	19.31744	19.07449	30.438105
60-64	31.109101667548682	19.059559410469063	18.983491031537124	30.847847890445134
65-69	30.942096512945692	19.15208143567317	19.130269594753795	30.77555245662734
70-74	31.053295	19.23583	19.08456	30.626314999999998
75-79	31.086605	19.071715	18.9745	30.86718
80-84	30.85305830325742	19.083201524644117	19.145186692004067	30.918553480094396
85-89	31.01800364912278	19.245534939908083	19.120562310329046	30.615899100640092
90-94	31.08003913844417	18.98704097850311	18.96956899455933	30.96335088849339
95-99	30.932555170687582	19.045409754393162	19.121990014766048	30.900045060153204
100-101	31.24975	19.333975	19.019175	30.3971
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.618243356455885
2	55.41435
3	57.786325000000005
4	59.137474999999995
5	58.585725
6	62.703399999999995
7	61.241975000000004
8	60.077775
9	62.44165
10-14	60.77235127032294
15-19	61.428285
20-24	61.39850257225549
25-29	61.35931240962936
30-34	61.99588647807972
35-39	61.609516283240836
40-44	61.64538712468997
45-49	61.90027500000001
50-54	61.688759999999995
55-59	61.608070000000005
60-64	61.95694955799381
65-69	61.717648969573034
70-74	61.67961
75-79	61.953784999999996
80-84	61.77161178335181
85-89	61.63390274976287
90-94	62.04339002693756
95-99	61.83260023084078
100-101	61.64685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.5
2	4.5
3	5.0
4	4.5
5	5.0
6	6.5
7	6.5
8	10.5
9	12.5
10	19.5
11	26.0
12	36.0
13	53.0
14	63.0
15	78.0
16	98.0
17	115.0
18	148.5
19	215.0
20	267.5
21	331.0
22	427.5
23	523.5
24	619.5
25	775.0
26	965.0
27	1137.5
28	1352.0
29	1652.5
30	2014.0
31	2372.0
32	2764.0
33	3258.0
34	3805.5
35	4360.0
36	4867.0
37	5547.0
38	6432.5
39	7377.0
40	8484.5
41	9747.5
42	11425.5
43	13651.0
44	16430.5
45	19901.5
46	24377.5
47	30099.5
48	37223.5
49	45825.0
50	55774.0
51	67575.0
52	80525.0
53	93880.0
54	108210.5
55	122637.5
56	136138.0
57	149364.0
58	161749.0
59	172519.0
60	181848.0
61	189000.0
62	194310.0
63	197559.0
64	198512.5
65	196341.5
66	190591.5
67	182450.0
68	171033.0
69	157197.5
70	141623.0
71	124105.0
72	105955.0
73	88049.5
74	71326.0
75	55777.5
76	42335.5
77	31352.5
78	22168.5
79	14994.0
80	9862.5
81	6277.0
82	3740.5
83	2254.0
84	1393.5
85	874.5
86	552.0
87	360.5
88	268.5
89	196.5
90	124.5
91	91.5
92	68.5
93	43.5
94	30.0
95	17.0
96	8.5
97	4.5
98	3.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032650000000000005
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	3.5E-5
15-19	0.0
20-24	0.001185
25-29	0.00209
30-34	0.04186
35-39	0.020210000000000002
40-44	0.0045049999999999995
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.011014999999999999
65-69	0.008440000000000001
70-74	0.0
75-79	0.0
80-84	2.7E-4
85-89	0.006105000000000001
90-94	0.011354999999999999
95-99	3.4E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15297246495630826
#Duplication Level	Relative count
1	100.0
2	0.13317979272017222
3	0.001001351824963701
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.001001351824963701
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	324680	3.2472055	3.7089353	85-89
AAAAA	323075	3.082078	3.517057	1
>>END_MODULE