##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92277475	34.0	31.0	34.0	31.0	34.0
2	33.13708675	34.0	33.0	34.0	31.0	34.0
3	33.17807975	34.0	34.0	34.0	31.0	34.0
4	36.524279	37.0	37.0	37.0	35.0	37.0
5	36.47006075	37.0	37.0	37.0	35.0	37.0
6	36.45183825	37.0	37.0	37.0	35.0	37.0
7	36.4393965	37.0	37.0	37.0	35.0	37.0
8	36.42726675	37.0	37.0	37.0	35.0	37.0
9	38.23826625	39.0	39.0	39.0	37.0	39.0
10-14	38.5103216	39.4	39.2	39.4	37.2	39.4
15-19	39.683832699999996	41.0	40.0	41.0	37.2	41.0
20-24	39.465425200000006	41.0	39.6	41.0	36.6	41.0
25-29	39.036224000000004	40.2	38.6	41.0	35.2	41.0
30-34	38.328204400000004	40.0	37.6	41.0	34.4	41.0
35-39	37.358019199999994	39.0	35.4	40.6	33.0	41.0
40-44	36.5257223	37.4	35.0	40.0	32.8	41.0
45-49	35.8356133	35.4	35.0	39.2	33.0	41.0
50-54	34.898655899999994	35.0	35.0	36.8	31.8	40.0
55-59	34.157644250000004	35.0	34.4	35.2	31.0	38.2
60-64	33.6293375	35.0	34.0	35.0	30.8	36.4
65-69	33.2293387	35.0	33.4	35.0	30.0	35.0
70-74	32.9628546	35.0	33.0	35.0	29.2	35.0
75-79	32.32224625	34.4	32.6	35.0	28.0	35.0
80-84	32.5943087	35.0	33.0	35.0	29.0	35.0
85-89	32.2904805	35.0	33.0	35.0	27.4	35.0
90-94	31.893230900000002	35.0	33.0	35.0	27.0	35.0
95-99	31.354589849999996	35.0	33.0	35.0	24.4	35.0
100-101	30.003813875	34.0	30.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31.0
3	11.0
4	24.0
5	69.0
6	261.0
7	635.0
8	1527.0
9	2535.0
10	3221.0
11	3767.0
12	4065.0
13	4323.0
14	4692.0
15	5065.0
16	5666.0
17	6334.0
18	6963.0
19	7550.0
20	8056.0
21	8951.0
22	10718.0
23	13243.0
24	16382.0
25	20516.0
26	26082.0
27	32741.0
28	40745.0
29	51511.0
30	64354.0
31	83796.0
32	111830.0
33	159040.0
34	264824.0
35	667651.0
36	1469214.0
37	783774.0
38	102642.0
39	7164.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.49990609596017	24.656700084501132	13.709214574607199	23.134179244931495
2	30.165375	24.60195	19.900575	25.3321
3	28.9742	19.9919	22.147824999999997	28.886074999999998
4	30.335475000000002	20.24025	20.5201	28.904175
5	31.041800000000002	21.09555	20.281650000000003	27.581
6	29.359600000000004	19.5255	17.76635	33.348549999999996
7	32.477875	19.1511	19.6414	28.729625
8	28.333775	19.849275	20.0295	31.78745
9	30.556825	18.1908	19.349225	31.903150000000004
10-14	30.437244565586685	19.64952294742844	19.595122939268443	30.318109547716432
15-19	30.680954999999997	19.15585	19.385659999999998	30.777535
20-24	30.599136829525257	19.208185564502063	19.37259565492761	30.820081951045076
25-29	30.73250275533183	19.35355980072608	19.311608554774075	30.602328889168007
30-34	30.903115091968942	19.117679139603144	19.10745214024478	30.87175362818313
35-39	30.743373401911068	19.185098665838783	19.25273089374559	30.818797038504563
40-44	30.86343943045749	19.253760231876278	19.17885384985353	30.7039464878127
45-49	30.996865	19.025705000000002	19.026139999999998	30.951289999999997
50-54	30.790553966629915	19.10221292750304	19.18235838032485	30.9248747255422
55-59	30.910700000000002	19.186455	19.14724	30.755605000000003
60-64	31.004706749461924	19.002615661075627	19.009522934950184	30.983154654512262
65-69	30.826171342392882	19.0869834528449	19.16120926858361	30.9256359361786
70-74	30.946285	19.190655	19.093375	30.769685000000003
75-79	31.049055	18.975405000000002	18.973275	31.002265
80-84	30.85508229137034	19.060333403822337	19.14498021365819	30.939604091149132
85-89	30.952174696335966	19.155009377481104	19.116512139209053	30.77630378697388
90-94	31.078098832508594	18.951067067764285	18.973717474493657	30.997116625233463
95-99	30.89302395359304	19.017952692903936	19.127344101615243	30.961679251887787
100-101	31.129371555515444	19.221487896077054	19.06996151339881	30.579179035008696
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.63408534089167
2	55.497474999999994
3	57.86027500000001
4	59.23965
5	58.6228
6	62.708149999999996
7	61.2075
8	60.121225
9	62.459975
10-14	60.75535411330312
15-19	61.45849
20-24	61.419218780570326
25-29	61.33483164449984
30-34	61.77486872015208
35-39	61.56217044041563
40-44	61.56738591827019
45-49	61.94815500000001
50-54	61.71542869217211
55-59	61.666305
60-64	61.987861403974186
65-69	61.75180727857149
70-74	61.71597
75-79	62.05132
80-84	61.79468638251947
85-89	61.72847848330984
90-94	62.07521545774206
95-99	61.854703205480824
100-101	61.70855059052413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.5
2	4.5
3	2.5
4	1.0
5	4.0
6	5.0
7	5.0
8	10.0
9	14.0
10	15.0
11	20.5
12	31.5
13	41.5
14	50.5
15	69.0
16	101.0
17	120.0
18	147.5
19	186.5
20	240.5
21	311.0
22	396.0
23	500.0
24	651.0
25	794.5
26	916.0
27	1138.5
28	1385.5
29	1690.0
30	1968.0
31	2229.0
32	2664.5
33	3208.5
34	3741.5
35	4288.5
36	4885.0
37	5581.5
38	6359.0
39	7267.5
40	8452.0
41	9880.0
42	11562.5
43	13583.5
44	16249.5
45	19866.5
46	24576.5
47	30372.5
48	37391.0
49	45876.5
50	56175.5
51	67717.5
52	80428.0
53	94299.0
54	108559.0
55	122808.0
56	136706.0
57	149580.5
58	161077.5
59	171651.5
60	180940.5
61	188768.0
62	194526.5
63	197589.5
64	197427.0
65	195192.0
66	190648.5
67	181968.0
68	170965.0
69	157485.0
70	141251.0
71	124246.5
72	106409.0
73	88487.0
74	71733.0
75	56438.5
76	42951.5
77	31449.0
78	22369.5
79	15354.5
80	9957.0
81	6289.5
82	3848.5
83	2211.0
84	1316.5
85	801.0
86	494.0
87	311.5
88	201.0
89	161.5
90	120.5
91	76.5
92	52.5
93	38.0
94	24.5
95	13.5
96	6.5
97	5.0
98	2.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030925
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.4999999999999999E-5
15-19	0.0
20-24	5.5E-5
25-29	0.00297
30-34	0.06844
35-39	0.01808
40-44	0.001845
45-49	0.0
50-54	5.65E-4
55-59	0.0
60-64	0.03292
65-69	0.03478
70-74	0.0
75-79	0.0
80-84	0.008045
85-89	0.03179
90-94	0.045945
95-99	0.015
100-101	9.125E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.17297491863679607
#Duplication Level	Relative count
1	100.0
2	0.15724924004547208
3	0.001001587516213198
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.00793758106599E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	302885	3.000855	3.6267447	10-14
>>END_MODULE