##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L002_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8549085	34.0	31.0	34.0	31.0	34.0
2	33.09443625	34.0	33.0	34.0	31.0	34.0
3	33.1329415	34.0	33.0	34.0	31.0	34.0
4	36.48435575	37.0	37.0	37.0	35.0	37.0
5	36.41976775	37.0	37.0	37.0	35.0	37.0
6	36.401797	37.0	37.0	37.0	35.0	37.0
7	36.39308	37.0	37.0	37.0	35.0	37.0
8	36.38071625	37.0	37.0	37.0	35.0	37.0
9	38.1832555	39.0	39.0	39.0	37.0	39.0
10-14	38.449645149999995	39.4	39.2	39.4	37.0	39.4
15-19	39.61363415	41.0	40.0	41.0	37.0	41.0
20-24	39.40276205	41.0	39.2	41.0	36.6	41.0
25-29	38.96166965	40.0	38.4	41.0	35.2	41.0
30-34	38.258956850000004	40.0	37.4	41.0	34.0	41.0
35-39	37.302759449999996	39.0	35.2	40.6	32.8	41.0
40-44	36.4619274	37.4	35.0	40.0	32.6	41.0
45-49	35.7392904	35.2	35.0	39.2	32.6	41.0
50-54	34.77766415	35.0	35.0	36.8	31.4	40.0
55-59	34.0495763	35.0	34.2	35.2	31.0	38.2
60-64	33.50766605	35.0	34.0	35.0	30.2	36.4
65-69	33.0724512	35.0	33.0	35.0	29.2	35.0
70-74	32.7982598	35.0	33.0	35.0	29.0	35.0
75-79	32.1029437	34.4	32.4	35.0	27.2	35.0
80-84	32.45062365	35.0	33.0	35.0	28.2	35.0
85-89	32.1140401	35.0	33.0	35.0	27.0	35.0
90-94	31.68283245	35.0	33.0	35.0	25.6	35.0
95-99	31.124430150000002	35.0	32.8	35.0	23.8	35.0
100-101	29.86850975	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	11.0
4	13.0
5	84.0
6	256.0
7	704.0
8	1708.0
9	2860.0
10	3705.0
11	4300.0
12	4634.0
13	4694.0
14	5374.0
15	5835.0
16	6442.0
17	7093.0
18	7965.0
19	8291.0
20	9070.0
21	9982.0
22	11931.0
23	14100.0
24	17627.0
25	22369.0
26	27885.0
27	34991.0
28	44288.0
29	55023.0
30	69667.0
31	89672.0
32	118659.0
33	167536.0
34	272986.0
35	657620.0
36	1424234.0
37	776864.0
38	103923.0
39	7540.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.468174551794135	24.659980680706287	13.77513652409248	23.096708243407097
2	30.138875	24.664375	19.88515	25.311600000000002
3	28.983999999999998	19.9833	22.165525	28.867175
4	30.29615	20.251375	20.563225	28.88925
5	31.0193	21.160225	20.2618	27.558675
6	29.29935	19.514975	17.788975	33.3967
7	32.467275	19.16805	19.629525	28.735149999999997
8	28.332575	19.85025	20.051225	31.765949999999997
9	30.50595	18.222175	19.374275	31.897599999999997
10-14	30.41959496506842	19.658535214630994	19.58997005693113	30.331899763369456
15-19	30.665100000000002	19.170245	19.378735	30.78592
20-24	30.58520956206271	19.235676388600762	19.371612564448682	30.807501484887844
25-29	30.721914454509147	19.366160844946872	19.327410713196425	30.584513987347556
30-34	30.895329057306103	19.118294525551097	19.124143210222442	30.862233206920358
35-39	30.731391881595822	19.173179433930827	19.242859426041694	30.85256925843166
40-44	30.851772612301314	19.25334076539539	19.193920269234248	30.70096635306905
45-49	30.993900000000004	19.030720000000002	19.044330000000002	30.93105
50-54	30.804647633185045	19.104323041676984	19.18355963037059	30.907469694767382
55-59	30.88937	19.20713	19.141025	30.762475000000002
60-64	31.025009490814725	18.99397713972675	19.00319414488622	30.977819224572308
65-69	30.81362602052467	19.075890596294187	19.165443602461472	30.945039780719668
70-74	30.964445	19.180349999999997	19.096799999999998	30.758405
75-79	31.054695	18.987535	18.98939	30.96838
80-84	30.86055296860652	19.074522733404955	19.135411764258794	30.92951253372973
85-89	30.951380722428084	19.17704880351993	19.107662851689604	30.763907622362378
90-94	31.09303152813589	18.967572186291125	18.937617936418764	31.00177834915422
95-99	30.92959230825274	19.019606048873285	19.109238358388126	30.941563284485852
100-101	31.138498284923138	19.242811158543454	19.03752193924624	30.58116861728717
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.564882795201235
2	55.450475
3	57.851175
4	59.1854
5	58.577975
6	62.69605
7	61.202425000000005
8	60.098525
9	62.403549999999996
10-14	60.75149472843787
15-19	61.45102
20-24	61.39271104695055
25-29	61.306428441856696
30-34	61.757562264226465
35-39	61.583961140027476
40-44	61.55273896537036
45-49	61.92495
50-54	61.71211732795242
55-59	61.65184500000001
60-64	62.00282871538704
65-69	61.75866580124434
70-74	61.722849999999994
75-79	62.023075000000006
80-84	61.790065502336255
85-89	61.715288344790466
90-94	62.0948098772901
95-99	61.87115559273859
100-101	61.7196669022103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	3.5
2	3.5
3	6.0
4	6.5
5	6.5
6	4.5
7	5.5
8	8.5
9	14.0
10	16.0
11	18.0
12	24.5
13	35.5
14	54.5
15	72.0
16	96.5
17	142.0
18	185.0
19	221.5
20	250.0
21	313.0
22	413.0
23	501.0
24	601.0
25	774.0
26	955.5
27	1132.5
28	1386.5
29	1683.0
30	2034.0
31	2348.5
32	2671.0
33	3178.5
34	3747.0
35	4340.0
36	4985.5
37	5702.0
38	6438.0
39	7311.0
40	8420.5
41	9629.5
42	11283.5
43	13618.0
44	16489.0
45	20054.0
46	24574.0
47	30242.5
48	37338.5
49	45983.0
50	56259.5
51	67992.5
52	80474.0
53	93944.0
54	108267.5
55	122722.0
56	136652.5
57	149639.5
58	161399.0
59	172215.5
60	181759.5
61	188847.5
62	194198.0
63	197178.0
64	197588.0
65	195654.0
66	189859.0
67	181794.5
68	171282.0
69	157226.0
70	141352.5
71	124047.5
72	106110.0
73	88355.5
74	71518.5
75	56194.0
76	42707.0
77	31476.0
78	22181.5
79	15060.5
80	10102.0
81	6461.5
82	3949.0
83	2328.0
84	1355.0
85	849.5
86	536.5
87	357.5
88	246.5
89	177.0
90	115.0
91	84.5
92	63.0
93	43.0
94	25.5
95	12.5
96	5.0
97	4.0
98	4.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022225
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.3E-4
15-19	0.0
20-24	8.65E-4
25-29	3.4E-4
30-34	0.063
35-39	0.014339999999999999
40-44	8.35E-4
45-49	0.0
50-54	0.002005
55-59	0.0
60-64	0.021755
65-69	0.02569
70-74	0.0
75-79	0.0
80-84	0.00662
85-89	0.02299
90-94	0.03088
95-99	0.008155
100-101	8.375E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.16698330166983652
#Duplication Level	Relative count
1	100.0
2	0.15424216019150067
3	0.0015023587031639675
4	5.007862343879892E-4
5	5.007862343879892E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007862343879892E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	306645	3.0498354	3.5166109	10-14
>>END_MODULE