##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_R2_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	65793958	
Filtered Sequences	0	
Sequence length	30-101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76893432980579	34.0	31.0	34.0	31.0	34.0
2	32.87381202997394	34.0	31.0	34.0	31.0	34.0
3	32.91134629717823	34.0	31.0	34.0	31.0	34.0
4	36.281236356079994	37.0	37.0	37.0	35.0	37.0
5	36.27516961359886	37.0	37.0	37.0	35.0	37.0
6	36.26568473050367	37.0	37.0	37.0	35.0	37.0
7	36.26797715984802	37.0	37.0	37.0	35.0	37.0
8	36.252628896410215	37.0	37.0	37.0	35.0	37.0
9	38.02482103295868	39.0	39.0	39.0	37.0	39.0
10-14	38.299770653712606	39.4	39.2	39.4	35.8	39.4
15-19	39.45282054926685	41.0	39.8	41.0	36.8	41.0
20-24	39.18610238952336	41.0	39.0	41.0	35.6	41.0
25-29	38.83028740724186	40.2	38.2	41.0	35.0	41.0
30-34	38.13833969130764	39.8	36.8	41.0	33.8	41.0
35-39	37.086983624628914	38.4	35.0	40.6	32.6	41.0
40-44	36.11542636419633	36.0	35.0	39.8	32.0	41.0
45-49	35.2018273425186	35.0	35.0	38.0	31.0	40.8
50-54	33.99286901138494	34.8	33.2	35.4	29.6	39.2
55-59	34.273624007822484	35.0	35.0	35.0	31.0	37.6
60-64	34.02081309771228	35.0	34.6	35.0	31.0	35.8
65-69	33.809941776777734	35.0	34.0	35.0	31.0	35.0
70-74	33.64395298176798	35.0	34.0	35.0	30.4	35.0
75-79	33.48075641589874	35.0	33.4	35.0	30.0	35.0
80-84	33.34583241509763	35.0	33.0	35.0	29.2	35.0
85-89	33.23505566024128	35.0	33.0	35.0	29.0	35.0
90-94	33.131069091257736	35.0	33.0	35.0	29.0	35.0
95-99	33.167100136869365	35.0	33.0	35.0	29.0	35.0
100-101	32.87902494001422	34.5	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	36.0
17	126.0
18	791.0
19	4113.0
20	13669.0
21	34749.0
22	74033.0
23	136747.0
24	225422.0
25	337487.0
26	471539.0
27	628849.0
28	808406.0
29	1020379.0
30	1278417.0
31	1628993.0
32	2146084.0
33	3036806.0
34	5035456.0
35	1.2127204E7
36	2.3786305E7
37	1.0779588E7
38	1950134.0
39	267442.0
40	1173.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.56937249465977	18.689985180706106	16.70647477994864	29.034167544685484
2	32.76275208913632	22.293078878870062	18.87685114121746	26.067317890776152
3	30.282502220724815	19.61066919563885	19.567583273152284	30.539245310484052
4	32.345663145607084	18.271566301805944	18.24424465630527	31.1385258962817
5	32.19756270656881	18.828182820206685	18.7435766622369	30.230677810987604
6	31.329534505921057	17.972375425255517	17.304260375247917	33.393829693575505
7	33.350817059738056	17.808523227675614	17.84679311145741	30.99386660112892
8	30.654917991631216	18.573258841804055	18.673430823702088	32.098392342862645
9	31.495713599380338	17.47100105491072	18.302698693009425	32.730586652699515
10-14	31.682742293497846	18.359848571219228	18.30901954059996	31.648389594682968
15-19	31.78980839831241	18.08112294368408	18.188423940398856	31.940644717604656
20-24	31.722979485365965	18.14628923026422	18.23010129312945	31.900629991240358
25-29	31.850215443756756	18.22275287931854	18.14381042327081	31.783221253653892
30-34	31.943806234248946	18.03368613162937	18.018108955179667	32.00439867894202
35-39	31.830401495775114	18.133163227048183	18.12146147965898	31.914973797517725
40-44	31.945638945961996	18.146301579820598	18.052466973016145	31.855592501201258
45-49	32.00515699393115	17.989520634062284	17.95983977627325	32.04548259573332
50-54	31.847172448405548	18.093064509078356	18.090547928245154	31.96921511427095
55-59	31.946783229648013	18.13030787262891	18.050731745175785	31.872177152547295
60-64	32.0005088274025	17.98914820295269	17.955552113067803	32.054790856577
65-69	31.82870974856524	18.106608848344756	18.10222616638538	31.962455236704624
70-74	31.924397184090438	18.157641209318935	18.06053674168833	31.857424864902296
75-79	31.97026648924562	18.03183647064925	17.978873463071757	32.01902357703337
80-84	31.789672228883564	18.141129798139666	18.13577265090292	31.933425322073848
85-89	31.863484013099658	18.20429740035041	18.104073443163397	31.82814514338654
90-94	31.875891196678808	18.08835977609704	18.047560729504927	31.988188297719223
95-99	31.642652237309534	18.210562901936264	18.225125383847658	31.921659476906544
100-101	32.07430889050969	18.142654275519256	17.95784099729713	31.825195836673924
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.60354003934525
2	58.830069979912466
3	60.82174753120887
4	63.48418904188878
5	62.42824051755642
6	64.72336419949656
7	64.34468366086698
8	62.75331033449386
9	64.22630025207987
10-14	63.33113188818081
15-19	63.730453115917065
20-24	63.623609476606326
25-29	63.63343669741065
30-34	63.94820491319096
35-39	63.74537529329284
40-44	63.801231447163254
45-49	64.05063958966447
50-54	63.81638756267649
55-59	63.8189603821953
60-64	64.0552996839795
65-69	63.791164985269866
70-74	63.78182204899273
75-79	63.98929006627899
80-84	63.72309755095742
85-89	63.691629156486194
90-94	63.86407949439803
95-99	63.56431171421608
100-101	63.89950472718362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	297.0
1	220.0
2	121.5
3	99.5
4	99.0
5	104.5
6	123.5
7	159.0
8	200.5
9	238.0
10	279.0
11	365.0
12	480.0
13	584.0
14	755.0
15	996.5
16	1287.0
17	1665.5
18	2196.5
19	2824.5
20	3758.5
21	4952.0
22	6424.5
23	8340.0
24	10915.0
25	14397.0
26	18511.5
27	23346.5
28	29371.5
29	36579.0
30	44875.5
31	54483.5
32	65545.5
33	78007.5
34	91464.0
35	105065.0
36	119223.5
37	134400.0
38	150035.5
39	165714.5
40	181600.0
41	198609.0
42	217749.5
43	239215.0
44	264204.5
45	294320.0
46	331462.0
47	378375.0
48	436692.0
49	507460.5
50	590468.5
51	688009.0
52	800807.0
53	929679.0
54	1075443.0
55	1235121.5
56	1408969.0
57	1597301.0
58	1802235.0
59	2022814.5
60	2254880.5
61	2498814.0
62	2744938.5
63	2984049.5
64	3206097.0
65	3390869.0
66	3528368.5
67	3601567.0
68	3596040.0
69	3511766.5
70	3348489.5
71	3107348.0
72	2801655.5
73	2452176.0
74	2076033.5
75	1700124.5
76	1341137.0
77	1013931.5
78	734740.0
79	507009.5
80	334015.5
81	209596.5
82	123968.5
83	70287.0
84	39270.0
85	22010.5
86	12619.5
87	7870.5
88	5161.0
89	3229.0
90	2087.0
91	1478.0
92	969.5
93	553.0
94	375.5
95	267.0
96	153.0
97	97.0
98	74.5
99	56.0
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009674140595098413
3	0.013876654145050826
4	0.022207206321285613
5	0.024683117559214176
6	0.03169743945181106
7	0.03682101022102972
8	0.045688085826969094
9	0.03998391463240439
10-14	0.01504393458134864
15-19	0.005627264436652375
20-24	0.0023093305923318976
25-29	0.0030157784397163035
30-34	0.011191714841859372
35-39	0.0010288384076309167
40-44	3.18326797124931E-4
45-49	2.8779499806292538E-5
50-54	2.9696976864628465E-4
55-59	6.494124169928456E-4
60-64	3.0807403606891996E-7
65-69	1.0350348514308966E-4
70-74	0.0
75-79	3.426030517042915E-6
80-84	3.1322851078574186E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	49891.0
32-33	53070.0
34-35	54888.0
36-37	56288.0
38-39	55656.0
40-41	56860.0
42-43	57022.0
44-45	56776.0
46-47	58161.0
48-49	58734.0
50-51	45640.0
52-53	40094.0
54-55	48662.0
56-57	54480.0
58-59	61371.0
60-61	65515.0
62-63	70829.0
64-65	75702.0
66-67	82653.0
68-69	89561.0
70-71	97890.0
72-73	107144.0
74-75	116842.0
76-77	128695.0
78-79	141026.0
80-81	155025.0
82-83	172725.0
84-85	196196.0
86-87	230697.0
88-89	272682.0
90-91	337963.0
92-93	426113.0
94-95	561398.0
96-97	759451.0
98-99	1118825.0
100-101	5.9779433E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	1.9516998030807386
#Duplication Level	Relative count
1	100.0
2	1.8228436564938806
3	0.0147825688026629
4	0.013253337547215015
5	0.018350775065374636
6	0.014272825050846939
7	0.0147825688026629
8	0.013253337547215015
9	0.009175387532687318
10++	0.028035906349877918
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GATCG	20738280	3.1025941	4.3475556	1
CGATC	20597165	3.0904622	3.4453087	6
TTTTT	3717320	3.0425785	3.4339151	10-14
ATCGA	11588525	3.040125	3.707176	2
TCGAT	11502600	3.030797	3.3783877	5
AAAAA	3775010	3.0230284	3.747382	1
>>END_MODULE