##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_R1_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	65793958	
Filtered Sequences	0	
Sequence length	30-101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.808968644202864	34.0	31.0	34.0	31.0	34.0
2	33.023823084180464	34.0	31.0	34.0	31.0	34.0
3	33.060930366888705	34.0	31.0	34.0	31.0	34.0
4	36.452834027708136	37.0	37.0	37.0	35.0	37.0
5	36.38808469312638	37.0	37.0	37.0	35.0	37.0
6	36.356640270828514	37.0	37.0	37.0	35.0	37.0
7	36.35165876477594	37.0	37.0	37.0	35.0	37.0
8	36.33267042545153	37.0	37.0	37.0	35.0	37.0
9	38.13072688224654	39.0	39.0	39.0	37.0	39.0
10-14	38.36870750958621	39.4	38.6	39.4	36.2	39.4
15-19	39.509482071286854	41.0	39.8	41.0	37.0	41.0
20-24	39.208474039515906	40.8	39.0	41.0	35.8	41.0
25-29	37.4845815781443	40.0	37.0	41.0	28.4	41.0
30-34	36.909670075323845	39.2	35.4	41.0	30.6	41.0
35-39	36.613913876498124	38.0	35.0	40.2	31.4	41.0
40-44	34.80032275581945	35.8	34.4	39.4	26.0	41.0
45-49	34.88939629884949	35.0	34.6	37.8	30.0	40.6
50-54	34.278616989756316	35.0	34.2	35.8	30.2	39.4
55-59	33.65796344228665	35.0	33.2	35.0	29.0	37.2
60-64	33.6141166572981	35.0	33.0	35.0	30.0	35.6
65-69	33.39622116185022	35.0	33.0	35.0	29.4	35.0
70-74	33.04142447321772	35.0	33.0	35.0	29.0	35.0
75-79	32.73274029792265	34.4	32.6	35.0	28.2	35.0
80-84	33.08393081448533	35.0	33.0	35.0	29.0	35.0
85-89	32.928670741436825	35.0	33.0	35.0	28.2	35.0
90-94	32.95791210283623	35.0	33.0	35.0	29.0	35.0
95-99	33.05223000958345	35.0	33.0	35.0	29.0	35.0
100-101	32.663748820441484	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	10.0
20	153.0
21	2329.0
22	16353.0
23	64532.0
24	166472.0
25	325971.0
26	529668.0
27	764061.0
28	1015298.0
29	1298206.0
30	1648348.0
31	2157593.0
32	3024278.0
33	4581152.0
34	7247143.0
35	1.3159173E7
36	2.0020343E7
37	8154625.0
38	1453821.0
39	164067.0
40	362.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.63089486119683	18.781068012354567	16.869783088593028	28.718254037855573
2	32.38025707872661	21.94267431748378	19.867671381422703	25.809397222366908
3	29.772237289208796	19.707629076025935	20.32232686658442	30.19780676818085
4	31.94841671534262	18.659586765342205	18.932809459423083	30.45918705989209
5	32.10845591627122	18.85061695178758	18.962990492227267	30.077936639713936
6	31.263562225577008	18.04258986820644	17.58993280203632	33.10391510418024
7	33.2701750516362	17.969678917933468	17.992450005819684	30.767696024610647
8	30.633229270079788	18.54767880053667	18.80237695990261	32.01671496948094
9	31.416257097650213	17.48380451591011	18.472253941615733	32.62768444482395
10-14	31.676893531855434	18.36041066851342	18.39023226388323	31.572463535747918
15-19	31.76840432673164	18.073660502382303	18.23977697161797	31.91815819926809
20-24	31.76304210600408	18.128660984667334	18.268894916521493	31.839401992807097
25-29	32.689162191469315	18.027425092918804	17.973834557020357	31.30957815859152
30-34	32.35361325876618	18.031621135702018	17.940939875122055	31.673825730409742
35-39	31.954893489228485	18.11164770564651	18.143109581444737	31.790349223680263
40-44	32.73708571781459	18.001541673415076	17.893871371365695	31.367501237404642
45-49	32.076892662882464	18.01877890381284	18.00046031912414	31.90386811418055
50-54	31.96063889455047	18.138281060050502	18.119926724202966	31.781153321196058
55-59	31.99940539121973	18.192538752734162	18.08193853440975	31.726117321636355
60-64	32.01997542209909	18.000926289127566	17.998455916862312	31.980642371911035
65-69	31.85743966350133	18.135809034563387	18.144503333575972	31.86224796835931
70-74	31.974155438420702	18.2052920752895	18.094654683428896	31.725897802860903
75-79	31.983741547737672	18.049235693743235	18.034013500815878	31.93300925770321
80-84	31.803047698259405	18.14885742286157	18.181106516507636	31.866988362371384
85-89	31.87931987820513	18.224602206504517	18.157502757651812	31.738575157638536
90-94	31.866431590591755	18.07467793563646	18.107505207719964	31.95138526605183
95-99	31.64490470270164	18.18465208947377	18.285513808177686	31.8849293996469
100-101	32.275322382574714	17.954071887525515	17.993809865755836	31.776795864143935
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	64.3491488990524
2	58.18965430109352
3	59.97004405738965
4	62.40760377523471
5	62.18639255598516
6	64.36747732975724
7	64.03787107624684
8	62.64994423956072
9	64.04394154247416
10-14	63.24935706760335
15-19	63.686562525999726
20-24	63.602444098811176
25-29	63.99874035006084
30-34	64.02743898917592
35-39	63.745242712908755
40-44	64.10458695521923
45-49	63.98076077706302
50-54	63.74179221574653
55-59	63.72552271285608
60-64	64.00061779401011
65-69	63.71968763186063
70-74	63.70005324128161
75-79	63.916750805440884
80-84	63.670036060630785
85-89	63.61789503584367
90-94	63.81781685664358
95-99	63.529834102348545
100-101	64.05211824671865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	91.0
2	89.5
3	88.5
4	76.5
5	82.0
6	104.5
7	128.5
8	146.0
9	174.0
10	232.5
11	325.5
12	437.0
13	556.0
14	737.5
15	985.0
16	1252.5
17	1576.5
18	2058.5
19	2679.0
20	3496.5
21	4632.5
22	6095.0
23	7874.5
24	10303.5
25	13762.5
26	17688.5
27	22284.0
28	28317.5
29	35270.0
30	43332.5
31	53354.0
32	64529.5
33	76844.5
34	90330.5
35	103918.0
36	117988.5
37	133256.0
38	149379.0
39	165461.5
40	181958.5
41	199470.0
42	218339.5
43	239385.5
44	264471.0
45	295390.0
46	333772.0
47	381685.0
48	439941.0
49	510275.0
50	594936.0
51	696443.0
52	811763.0
53	942493.5
54	1090699.0
55	1252579.0
56	1428314.0
57	1617877.5
58	1824732.0
59	2050582.5
60	2288060.5
61	2533660.5
62	2778992.5
63	3017585.0
64	3237765.0
65	3421140.0
66	3558066.5
67	3624776.5
68	3611837.5
69	3523133.5
70	3352600.5
71	3102727.5
72	2791001.0
73	2434918.5
74	2055075.5
75	1678518.5
76	1321710.5
77	998659.0
78	722954.0
79	499492.5
80	329913.0
81	208328.5
82	124448.5
83	71330.5
84	40711.5
85	23396.0
86	13427.0
87	8270.0
88	5405.5
89	3364.5
90	2159.0
91	1501.0
92	941.5
93	494.0
94	320.5
95	200.5
96	96.0
97	66.5
98	45.0
99	21.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.1037202838594994E-5
3	1.5198964014294442E-6
4	1.3679067612865E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.042924427802322E-5
15-19	0.0
20-24	0.0015761325682823338
25-29	0.21592773002043741
30-34	0.2501249427546314
35-39	0.01859700604393092
40-44	0.31462839942052745
45-49	0.0
50-54	6.739379679111532E-4
55-59	0.09891368367769224
60-64	0.012522486389747792
65-69	0.01202769431694019
70-74	6.483635668277773E-5
75-79	0.0
80-84	3.7105077361456184E-4
85-89	0.0017599847690327297
90-94	0.006762005151009197
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	55077.0
32-33	56077.0
34-35	56946.0
36-37	61631.0
38-39	66480.0
40-41	64427.0
42-43	67178.0
44-45	55942.0
46-47	60734.0
48-49	66580.0
50-51	71685.0
52-53	76041.0
54-55	76671.0
56-57	80920.0
58-59	85271.0
60-61	88670.0
62-63	92311.0
64-65	100375.0
66-67	110121.0
68-69	117667.0
70-71	120448.0
72-73	126231.0
74-75	143130.0
76-77	94557.0
78-79	135152.0
80-81	161479.0
82-83	188865.0
84-85	213227.0
86-87	261484.0
88-89	275821.0
90-91	344086.0
92-93	440972.0
94-95	586563.0
96-97	918598.0
98-99	1264378.0
100-101	5.9008163E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	1.6213702903767742
#Duplication Level	Relative count
1	100.0
2	1.5134190158202598
3	0.014737794311211395
4	0.019311592545725276
5	0.014229594507376521
6	0.019819792349560154
7	0.015245994115046272
8	0.013721394703541645
9	0.007622997057523136
10++	0.021852591564899656
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GATCG	20511145	3.0700524	4.399213	1
CGATC	20179775	3.055367	3.453399	1
ATCGA	11428165	3.0110164	3.704325	2
>>END_MODULE