##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L003_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1814064	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33276995739952	34.0	31.0	34.0	31.0	34.0
2	32.475838779668194	34.0	31.0	34.0	31.0	34.0
3	32.53990818405525	34.0	31.0	34.0	31.0	34.0
4	35.96641408461885	37.0	37.0	37.0	35.0	37.0
5	35.966767986135	37.0	37.0	37.0	35.0	37.0
6	35.9428680575768	37.0	37.0	37.0	35.0	37.0
7	35.9474467273481	37.0	37.0	37.0	35.0	37.0
8	35.90052335529507	37.0	37.0	37.0	35.0	37.0
9	37.61247894230854	39.0	38.0	39.0	35.0	39.0
10-14	37.868945968830204	39.4	38.2	39.4	35.2	39.4
15-19	38.905420977429685	41.0	39.0	41.0	35.4	41.0
20-24	38.60081728097796	40.0	38.0	41.0	34.2	41.0
25-29	38.129136348000955	40.0	37.8	41.0	33.2	41.0
30-34	37.422117080764515	39.6	36.0	41.0	32.4	41.0
35-39	36.3580453611339	38.0	35.0	40.4	31.0	41.0
40-44	35.38647721359335	35.8	35.0	39.6	30.2	41.0
45-49	34.44203986187919	35.0	34.2	37.6	29.4	40.6
50-54	33.18058117023435	34.8	32.8	35.2	26.8	39.2
55-59	33.31062123497296	35.0	33.8	35.0	28.2	37.0
60-64	33.06237442559909	35.0	33.6	35.0	27.4	35.6
65-69	32.773640952028146	35.0	33.0	35.0	28.2	35.0
70-74	32.501337218532534	35.0	33.0	35.0	26.6	35.0
75-79	32.26435252559998	35.0	33.0	35.0	27.0	35.0
80-84	31.958745887686433	35.0	33.0	35.0	25.8	35.0
85-89	31.655201911288685	35.0	33.0	35.0	24.4	35.0
90-94	31.22560427857011	35.0	32.6	35.0	23.8	35.0
95-99	30.618511364538403	35.0	31.6	35.0	20.2	35.0
100-101	29.38502969024246	34.0	30.0	35.0	8.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2987.0
3	670.0
4	824.0
5	1148.0
6	1577.0
7	1905.0
8	2347.0
9	2653.0
10	2846.0
11	2871.0
12	2965.0
13	2896.0
14	2899.0
15	2999.0
16	3266.0
17	3619.0
18	4052.0
19	4795.0
20	5605.0
21	6709.0
22	8187.0
23	9888.0
24	12133.0
25	14568.0
26	17208.0
27	21385.0
28	25480.0
29	30888.0
30	38434.0
31	47832.0
32	61753.0
33	85215.0
34	136806.0
35	321027.0
36	610435.0
37	262083.0
38	45714.0
39	5365.0
40	30.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.532021076256676	18.68800026469396	16.76488834602118	29.015090313028182
2	32.81107993399864	22.29658037077234	18.85709779318986	26.035241902039157
3	30.28501093820397	19.639521796170552	19.507667771975807	30.567799493649666
4	32.35081492983889	18.3053032613185	18.217625997466733	31.126255811375874
5	32.19287261675745	18.870643943466582	18.728996119985467	30.2074873197905
6	31.31926351200117	18.02221283793096	17.24808231518061	33.41044133488725
7	33.36488107549121	17.822488796966564	17.78994829369183	31.022681833850395
8	30.68654305883599	18.621651705684364	18.589659436781623	32.102145798698025
9	31.48755005191386	17.569118258783703	18.244439810263387	32.69889187903905
10-14	31.721925572463945	18.355151409012215	18.27780974767407	31.645113270849773
15-19	31.80859744760276	18.06848308744845	18.182871916163972	31.94004754878482
20-24	31.76007737248476	18.143069187219513	18.2123943513499	31.884459088945828
25-29	31.88176257616005	18.260426371663794	18.099088863764255	31.75872218841191
30-34	31.97347594894494	18.043033482936504	18.001180915140857	31.982309652977698
35-39	31.841165728281894	18.11590039782339	18.098246068272744	31.944687805621975
40-44	31.973671487224465	18.12526937638313	18.04879377266304	31.85226536372937
45-49	32.07793814405709	17.958402823305057	17.925876775453617	32.03778225718423
50-54	31.90089958566838	18.06627453505676	18.061930095542383	31.970895783732477
55-59	31.988091558985033	18.097449919067188	17.9955079836324	31.918950538315382
60-64	32.07902257031726	17.956929854735005	17.884220181867892	32.079827393079846
65-69	31.903946057029962	18.07057523880084	18.03407156528105	31.991407138888157
70-74	32.00691927076443	18.11091560165463	17.974172906799318	31.90799222078163
75-79	32.089299568403575	17.953593268740686	17.899846300207525	32.05726086264822
80-84	31.94002455537484	18.07780322889067	18.021177687772763	31.960994527961727
85-89	32.04125102532215	18.099934732181445	17.981372211785253	31.877442030711155
90-94	32.086001375916176	17.958506425352137	17.882665661189463	32.07282653754223
95-99	31.960488384802755	18.041656013582145	18.009197870419463	31.988657731195637
100-101	32.17387972879596	18.15203313454335	17.912046386596682	31.762040750064013
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.54711138928486
2	58.846321836037795
3	60.85281043185364
4	63.47707074121477
5	62.40035993654796
6	64.72970484688842
7	64.38756290934161
8	62.78868885753401
9	64.18644193095291
10-14	63.367038843313715
15-19	63.74864499638757
20-24	63.64453646143059
25-29	63.64048476457196
30-34	63.955785601922635
35-39	63.78585353390387
40-44	63.82593685095384
45-49	64.11572040124133
50-54	63.87179536940085
55-59	63.90704209730041
60-64	64.1588499633971
65-69	63.89535319591811
70-74	63.91491149154606
75-79	64.1465604310518
80-84	63.901019083336564
85-89	63.918693056033305
90-94	64.15882791345841
95-99	63.94914611599839
100-101	63.93592047885998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	38.5
2	24.0
3	24.0
4	17.5
5	12.0
6	16.0
7	19.5
8	17.0
9	18.0
10	19.0
11	20.5
12	31.0
13	38.0
14	37.5
15	46.0
16	56.0
17	71.5
18	87.5
19	101.0
20	133.5
21	170.5
22	188.5
23	239.5
24	328.0
25	412.5
26	508.0
27	653.5
28	870.5
29	1075.5
30	1267.0
31	1540.0
32	1817.5
33	2131.5
34	2526.0
35	2894.0
36	3271.5
37	3694.5
38	4086.5
39	4500.5
40	5002.5
41	5439.5
42	5943.0
43	6548.0
44	7172.0
45	7941.5
46	8891.0
47	10160.0
48	11847.5
49	13664.0
50	15760.5
51	18389.0
52	21497.5
53	24936.0
54	28709.5
55	32944.5
56	37632.5
57	42577.5
58	48018.0
59	53962.0
60	60299.5
61	66712.5
62	73014.5
63	79786.5
64	85881.0
65	90614.5
66	94369.5
67	96542.5
68	96640.5
69	94510.5
70	90009.0
71	83338.5
72	75420.5
73	66122.0
74	55934.0
75	45917.0
76	36113.0
77	27471.0
78	20032.5
79	13690.0
80	8930.0
81	5552.0
82	3225.0
83	1741.5
84	886.5
85	466.5
86	273.0
87	171.0
88	104.0
89	60.5
90	42.5
91	29.5
92	21.5
93	21.0
94	17.0
95	14.0
96	9.0
97	4.5
98	5.0
99	3.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03577602554264899
2	0.042335882306247186
3	0.03831176849328359
4	0.032799283817990986
5	0.022656312015452595
6	0.008709725786962313
7	0.05176223110099754
8	0.06207057744379471
9	0.07971052840473103
10-14	0.030450965346316337
15-19	0.033085933021106205
20-24	0.016129530159906154
25-29	0.019326771271575865
30-34	0.030605314917224527
35-39	0.01899602219105831
40-44	0.008390001675795341
45-49	0.007342629587489747
50-54	0.013847361504335017
55-59	0.004972261177113928
60-64	0.0
65-69	0.0
70-74	0.0
75-79	4.520237433739934E-4
80-84	0.0022931936249217227
85-89	0.0
90-94	0.0
95-99	0.0019514195750535814
100-101	3.0318665714109316E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1814064.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15591580546505127
#Duplication Level	Relative count
1	100.0
2	0.09709904102184228
3	0.002002042082924583
4	0.0
5	5.005105207311457E-4
6	0.0
7	0.0
8	0.0
9	5.005105207311457E-4
10++	0.002002042082924583
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	123965	3.5807302	4.4559245	2
TTTTT	109515	3.264511	3.6540823	80-84
GATCG	577830	3.0821688	4.3135033	1
CGATC	573655	3.073582	3.400914	6
ATCGA	322980	3.0361834	3.844308	2
TCGAT	320610	3.0329401	3.57617	8
>>END_MODULE