##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L003_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5546215	34.0	31.0	34.0	31.0	34.0
2	32.7169245	34.0	31.0	34.0	31.0	34.0
3	32.75978325	34.0	31.0	34.0	31.0	34.0
4	36.13912325	37.0	37.0	37.0	35.0	37.0
5	36.145469	37.0	37.0	37.0	35.0	37.0
6	36.14139075	37.0	37.0	37.0	35.0	37.0
7	36.14193475	37.0	37.0	37.0	35.0	37.0
8	36.11802375	37.0	37.0	37.0	35.0	37.0
9	37.8684455	39.0	39.0	39.0	35.0	39.0
10-14	38.041673349999996	39.4	38.6	39.4	35.2	39.4
15-19	39.24445475	41.0	39.6	41.0	36.0	41.0
20-24	38.9452206	41.0	39.0	41.0	35.6	41.0
25-29	38.52414395	40.0	38.2	41.0	34.8	41.0
30-34	37.7654629	39.8	36.6	41.0	33.2	41.0
35-39	36.58177465	38.2	35.0	40.6	31.8	41.0
40-44	35.615238899999994	36.0	35.0	39.6	31.0	41.0
45-49	34.632740999999996	35.0	34.6	38.0	30.4	40.6
50-54	33.32301105	34.8	33.2	35.2	27.8	39.2
55-59	33.505923900000006	35.0	34.0	35.0	30.0	37.4
60-64	33.208050300000004	35.0	34.0	35.0	29.4	35.6
65-69	32.911571249999994	35.0	33.8	35.0	29.0	35.0
70-74	32.6157139	35.0	33.0	35.0	28.2	35.0
75-79	32.17211435	35.0	33.0	35.0	27.0	35.0
80-84	31.975606600000003	35.0	33.0	35.0	26.2	35.0
85-89	31.6481827	35.0	33.0	35.0	24.4	35.0
90-94	31.1936914	35.0	32.8	35.0	23.8	35.0
95-99	30.526570299999996	35.0	31.6	35.0	19.0	35.0
100-101	29.303158625000002	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8169.0
3	1421.0
4	2024.0
5	2637.0
6	3326.0
7	4138.0
8	4970.0
9	5571.0
10	5887.0
11	6277.0
12	6347.0
13	6437.0
14	6507.0
15	6511.0
16	6993.0
17	7778.0
18	8601.0
19	9962.0
20	11478.0
21	13222.0
22	15936.0
23	19475.0
24	23193.0
25	27990.0
26	33366.0
27	40681.0
28	49202.0
29	60616.0
30	74944.0
31	96117.0
32	127366.0
33	179642.0
34	300162.0
35	714984.0
36	1369092.0
37	616290.0
38	109209.0
39	13413.0
40	66.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.5613877861346	18.752125667857	16.72024371571872	28.96624283028968
2	32.81093311530369	22.316830201409633	18.85275666595842	26.01948001732825
3	30.320062439489227	19.624947668891537	19.533510552178587	30.521479339440656
4	32.33945711848729	18.278352414475236	18.257429231588603	31.12476123544888
5	32.198241186854816	18.814071005606234	18.75574416762898	30.231943639909968
6	31.34303749646602	17.95992404132192	17.283976872133724	33.413061590078335
7	33.39855752773946	17.819691968316054	17.825747309126648	30.95600319481783
8	30.679254321124837	18.593018777118477	18.640545331548395	32.08718157020829
9	31.54419312231886	17.494983444176878	18.269123671460584	32.691699762043676
10-14	31.735981014673587	18.3940535830091	18.26339613998982	31.6065692623275
15-19	31.797855819784086	18.075095434139413	18.17510955177847	31.951939194298035
20-24	31.75212540183437	18.160657720396806	18.205834232411945	31.881382645356876
25-29	31.866918480599455	18.21588771588794	18.1325588382654	31.784634965247204
30-34	31.968702449637576	18.029141258527982	17.998452873116282	32.00370341871815
35-39	31.90524905099178	18.127353455123036	18.08062628016532	31.886771213719868
40-44	31.981829421014098	18.135780216614585	18.014399628706435	31.867990733664886
45-49	32.04654274045909	17.968194900526175	17.91536543348568	32.06989692552906
50-54	31.906419832921852	18.064495155874006	18.047245701202336	31.98183931000181
55-59	32.0055016780118	18.076658380806148	18.009272828212605	31.908567112969454
60-64	32.077249623174886	17.951430385429116	17.89967036990111	32.071649621494885
65-69	31.903549973342436	18.063667449618507	18.031162373231577	32.00162020380748
70-74	32.02517241762069	18.089957662970363	17.97757258430081	31.907297335108137
75-79	32.14305750210377	17.98114880206242	17.859562525406517	32.01623117042729
80-84	31.96366579564693	18.019902574335376	18.01096668789544	32.00546494212226
85-89	32.070243017560756	18.06861951715488	17.972014493003623	31.88912297228074
90-94	32.149925	17.903164999999998	17.86316	32.08375
95-99	32.00555628508995	18.01133079708728	17.98009194533686	32.003020972485906
100-101	32.24153360910949	18.102332363228975	17.88736847118745	31.768765556474087
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.52763061642428
2	58.83041313263194
3	60.84154177892987
4	63.46421835393616
5	62.43018482676479
6	64.75609908654437
7	64.3545607225573
8	62.76643589133313
9	64.23589288436253
10-14	63.34255027700109
15-19	63.749795014082125
20-24	63.63350804719124
25-29	63.65155344584665
30-34	63.97240586835573
35-39	63.792020264711645
40-44	63.84982015467898
45-49	64.11643966598814
50-54	63.888259142923665
55-59	63.91406879098125
60-64	64.14889924466978
65-69	63.90517017714992
70-74	63.93246975272883
75-79	64.15928867253106
80-84	63.969130737769184
85-89	63.9593659898415
90-94	64.233675
95-99	64.00857725757587
100-101	64.01029916558358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	81.0
2	41.5
3	36.0
4	34.5
5	33.5
6	34.0
7	35.5
8	34.0
9	42.5
10	46.5
11	52.5
12	76.0
13	90.5
14	85.5
15	103.5
16	149.0
17	194.0
18	233.0
19	286.5
20	332.5
21	397.5
22	516.5
23	632.5
24	775.0
25	963.0
26	1222.0
27	1551.0
28	1889.0
29	2312.0
30	2826.5
31	3419.5
32	4087.5
33	4798.5
34	5651.5
35	6486.5
36	7319.0
37	8195.5
38	9093.0
39	10120.0
40	11012.5
41	11877.0
42	13144.0
43	14361.0
44	15743.0
45	17647.5
46	19822.0
47	22459.0
48	25983.0
49	30124.0
50	34911.0
51	40736.0
52	47460.5
53	55075.0
54	63557.5
55	72645.5
56	82593.0
57	93693.5
58	105954.5
59	119129.0
60	132727.0
61	147064.5
62	161496.0
63	175831.5
64	188767.0
65	199448.5
66	207321.0
67	211782.0
68	212116.5
69	207090.5
70	197367.5
71	183478.0
72	165509.0
73	144908.5
74	123246.5
75	101108.0
76	79786.5
77	60790.5
78	44376.5
79	30644.5
80	20302.5
81	12885.0
82	7746.5
83	4435.0
84	2506.0
85	1490.0
86	952.5
87	641.5
88	452.0
89	334.5
90	266.0
91	203.0
92	160.0
93	128.0
94	103.5
95	85.5
96	60.5
97	44.5
98	37.5
99	21.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.104925
2	0.10532499999999999
3	0.095275
4	0.11080000000000001
5	0.088875
6	0.07725
7	0.0882
8	0.108475
9	0.118225
10-14	0.055444999999999994
15-19	0.05411
20-24	0.03655
25-29	0.034655
30-34	0.05991
35-39	0.015354999999999999
40-44	0.0252
45-49	0.01792
50-54	0.025825
55-59	0.0305
60-64	2.9999999999999997E-5
65-69	2.35E-4
70-74	7.0E-5
75-79	0.00105
80-84	0.00992
85-89	2.4999999999999998E-5
90-94	0.0
95-99	0.012329999999999999
100-101	0.0064624999999999995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.25287103577175873
#Duplication Level	Relative count
1	100.0
2	0.19038457684522736
3	0.009018216797931822
4	0.0010020240886590914
5	0.0
6	0.0
7	0.0010020240886590914
8	0.0
9	0.0
10++	0.0010020240886590914
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	272430	3.5728571	4.3666277	1
TTTTT	248750	3.365014	3.745863	10-14
GATCG	1273835	3.0808265	4.3139267	1
CGATC	1260190	3.0640924	3.4683807	1
TCGAT	703205	3.0180907	3.4071896	5
ATCGA	706540	3.0136614	3.670774	2
>>END_MODULE