##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L003_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.585774	34.0	31.0	34.0	31.0	34.0
2	32.740549	34.0	31.0	34.0	31.0	34.0
3	32.77086375	34.0	31.0	34.0	31.0	34.0
4	36.15689125	37.0	37.0	37.0	35.0	37.0
5	36.14885025	37.0	37.0	37.0	35.0	37.0
6	36.1538385	37.0	37.0	37.0	35.0	37.0
7	36.1587725	37.0	37.0	37.0	35.0	37.0
8	36.13191525	37.0	37.0	37.0	35.0	37.0
9	37.8839375	39.0	39.0	39.0	35.0	39.0
10-14	38.163419999999995	39.4	39.2	39.4	35.4	39.4
15-19	39.31401095	41.0	40.0	41.0	36.6	41.0
20-24	39.05953625	41.0	39.0	41.0	35.6	41.0
25-29	38.61456295000001	40.2	38.2	41.0	35.0	41.0
30-34	37.855624999999996	39.8	36.8	41.0	33.4	41.0
35-39	36.74470865000001	38.2	35.0	40.6	32.4	41.0
40-44	35.72729145	36.0	35.0	39.6	31.0	41.0
45-49	34.7590915	35.0	34.8	38.0	30.6	40.8
50-54	33.4516171	34.8	33.2	35.4	28.2	39.2
55-59	33.63455075	35.0	34.0	35.0	30.0	37.4
60-64	33.3405316	35.0	34.0	35.0	30.0	35.6
65-69	33.041352950000004	35.0	34.0	35.0	29.0	35.0
70-74	32.76051885	35.0	33.4	35.0	29.0	35.0
75-79	32.4904001	35.0	33.0	35.0	27.6	35.0
80-84	32.17103675	35.0	33.0	35.0	27.0	35.0
85-89	31.8369872	35.0	33.0	35.0	25.8	35.0
90-94	31.4139135	35.0	33.0	35.0	24.0	35.0
95-99	30.777056599999998	35.0	32.2	35.0	21.4	35.0
100-101	29.576935125	34.0	30.0	35.0	9.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7997.0
3	1301.0
4	1754.0
5	2181.0
6	3079.0
7	3728.0
8	4372.0
9	5064.0
10	5354.0
11	5602.0
12	5736.0
13	5778.0
14	5813.0
15	6007.0
16	6485.0
17	7132.0
18	8116.0
19	9200.0
20	10744.0
21	12667.0
22	14952.0
23	18154.0
24	22119.0
25	26531.0
26	31605.0
27	38421.0
28	47300.0
29	57236.0
30	71592.0
31	91312.0
32	120071.0
33	170562.0
34	284932.0
35	697444.0
36	1409901.0
37	650199.0
38	115066.0
39	14425.0
40	68.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.54801886799543	18.733868222148125	16.70873277345955	29.0093801363969
2	32.80248825488981	22.30518798940047	18.86191798638935	26.030405769320375
3	30.29071472892335	19.643741503295725	19.557293369295355	30.508250398485565
4	32.34746818337457	18.276089833232763	18.26346562947812	31.112976353914547
5	32.17618772436213	18.837288469298883	18.72629567368569	30.2602281326533
6	31.374019855190866	17.952017956973744	17.314373187883447	33.359588999951946
7	33.40723946337041	17.813237924507614	17.805628713557763	30.973893898564214
8	30.622542576970563	18.616183668200975	18.68266225846425	32.07861149636421
9	31.514128685561676	17.477802860046452	18.3022036612321	32.70586479315977
10-14	31.693978076498176	18.355498625608234	18.28683033843005	31.66369295946354
15-19	31.768072287950716	18.07016602655288	18.179029744172016	31.982731941324396
20-24	31.72764553843297	18.139439178350617	18.23721956115944	31.89569572205697
25-29	31.837878772416282	18.232512450907237	18.14880937730556	31.780799399370917
30-34	31.960056023264116	18.013776598100645	18.004567842876614	32.02159953575862
35-39	31.8304144868418	18.12121018666602	18.122430951963786	31.92594437452839
40-44	31.971164546703037	18.11018655886674	18.05425861158846	31.864390282841764
45-49	32.05119106084048	17.947701087250366	17.931230855481246	32.069876996427915
50-54	31.897779900427665	18.064538172654913	18.039200109616512	31.99848181730091
55-59	31.98646406312502	18.0964273436049	18.003264235235424	31.913844358034655
60-64	32.06430046108544	17.94769205030745	17.89277699813815	32.09523049046897
65-69	31.920795000000002	18.060415	18.020395	31.998395000000002
70-74	32.027465	18.101025	17.98224	31.889269999999996
75-79	32.084332436546795	17.954366640440597	17.874668392737004	32.08663253027561
80-84	31.935235333102995	18.036787904637272	18.036142806356548	31.991833955903182
85-89	32.056665524664155	18.088289794237546	17.949889489756877	31.90515519134142
90-94	32.12660291787115	17.934600025850187	17.86845454629546	32.0703425099832
95-99	31.992837237922377	17.9996298812905	17.989414364896145	32.01811851589098
100-101	32.20518195394502	18.130450686329244	17.89402769419326	31.77033966553247
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.55739900439232
2	58.832894024210184
3	60.79896512740892
4	63.46044453728912
5	62.436415857015426
6	64.7336088551428
7	64.38113336193462
8	62.70115407333478
9	64.21999347872145
10-14	63.357671035961715
15-19	63.750804229275104
20-24	63.62334126048994
25-29	63.6186781717872
30-34	63.98165555902274
35-39	63.75635886137019
40-44	63.835554829544805
45-49	64.12106805726839
50-54	63.89626171772858
55-59	63.90030842115968
60-64	64.1595309515544
65-69	63.91919
70-74	63.916735
75-79	64.1709649668224
80-84	63.92706928900618
85-89	63.961820716005576
90-94	64.19694542785436
95-99	64.01095575381336
100-101	63.97552161947749
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	65.5
2	28.0
3	24.0
4	22.0
5	18.5
6	22.0
7	27.0
8	33.5
9	35.5
10	37.5
11	46.0
12	62.5
13	78.0
14	92.5
15	123.5
16	155.5
17	194.0
18	231.0
19	281.0
20	369.5
21	461.0
22	547.0
23	656.0
24	810.5
25	983.0
26	1246.0
27	1580.5
28	1924.0
29	2296.5
30	2800.5
31	3449.5
32	4078.5
33	4817.5
34	5640.0
35	6413.5
36	7313.0
37	8218.0
38	9227.0
39	10213.5
40	11128.0
41	12160.5
42	13125.5
43	14411.5
44	15975.5
45	17733.5
46	19914.5
47	22748.0
48	26052.5
49	30056.5
50	35022.0
51	40736.0
52	47693.0
53	55238.0
54	63535.5
55	72857.0
56	82984.5
57	94235.5
58	106076.5
59	118879.0
60	132605.5
61	146881.0
62	161443.0
63	175652.5
64	188657.0
65	199236.0
66	207065.0
67	211218.5
68	211378.5
69	207097.0
70	197607.5
71	183597.5
72	165780.5
73	145777.5
74	123596.0
75	100999.5
76	79762.0
77	60335.5
78	43952.0
79	30545.0
80	20258.5
81	12786.0
82	7596.5
83	4210.5
84	2331.5
85	1375.0
86	852.5
87	549.0
88	388.5
89	299.0
90	229.5
91	188.0
92	146.5
93	105.5
94	78.5
95	64.0
96	45.0
97	28.5
98	20.5
99	14.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.206675
2	0.20315
3	0.200275
4	0.19172499999999998
5	0.196225
6	0.1168
7	0.12105
8	0.155825
9	0.17295
10-14	0.099445
15-19	0.09068
20-24	0.05664
25-29	0.051460000000000006
30-34	0.09507499999999999
35-39	0.06269
40-44	0.03209
45-49	0.031765
50-54	0.051555000000000004
55-59	0.040905
60-64	9.5E-5
65-69	0.0
70-74	0.0
75-79	0.0040750000000000005
80-84	0.015234999999999999
85-89	2.2E-4
90-94	7.25E-4
95-99	0.0050550000000000005
100-101	0.009725000000000001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.24086030102540973
#Duplication Level	Relative count
1	100.0
2	0.16930983700171315
3	0.008515583517837643
4	0.002003666710079445
5	0.0010018333550397226
6	0.0010018333550397226
7	5.009166775198613E-4
8	0.0
9	0.0
10++	0.0010018333550397226
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	274435	3.6039279	4.1970124	60-64
TTTTT	244595	3.2882218	3.5705283	9
GATCG	1276920	3.0905445	4.4434786	1
CGATC	1265680	3.0758262	3.5126166	1
ATCGA	710535	3.0307205	3.8095562	2
TCGAT	704365	3.0185163	3.4520237	8
>>END_MODULE