##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L003_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61042275	34.0	31.0	34.0	31.0	34.0
2	32.76761325	34.0	31.0	34.0	31.0	34.0
3	32.81248575	34.0	31.0	34.0	31.0	34.0
4	36.20297025	37.0	37.0	37.0	35.0	37.0
5	36.21957375	37.0	37.0	37.0	35.0	37.0
6	36.1689035	37.0	37.0	37.0	35.0	37.0
7	36.1687965	37.0	37.0	37.0	35.0	37.0
8	36.1321175	37.0	37.0	37.0	35.0	37.0
9	37.88545975	39.0	39.0	39.0	35.0	39.0
10-14	38.173299	39.4	39.0	39.4	35.4	39.4
15-19	39.33131515	41.0	39.8	41.0	36.6	41.0
20-24	39.09446935	41.0	39.0	41.0	35.6	41.0
25-29	38.6415305	40.2	38.2	41.0	35.0	41.0
30-34	37.871479699999995	39.8	36.8	41.0	33.4	41.0
35-39	36.796383500000005	38.2	35.0	40.6	32.6	41.0
40-44	35.768980600000006	36.0	35.0	39.6	31.4	41.0
45-49	34.7874628	35.0	34.8	38.0	30.6	40.6
50-54	33.46650115	34.8	33.2	35.2	28.2	39.2
55-59	33.72320345	35.0	34.8	35.0	30.4	37.4
60-64	33.3918984	35.0	34.0	35.0	30.0	35.6
65-69	33.085805500000006	35.0	34.0	35.0	29.2	35.0
70-74	32.816982949999996	35.0	33.2	35.0	29.0	35.0
75-79	32.5621664	35.0	33.0	35.0	28.0	35.0
80-84	32.23906905	35.0	33.0	35.0	27.0	35.0
85-89	31.88758625	35.0	33.0	35.0	26.2	35.0
90-94	31.445432400000005	35.0	33.0	35.0	24.0	35.0
95-99	30.822859599999997	35.0	32.2	35.0	21.8	35.0
100-101	29.610009124999998	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7079.0
3	1178.0
4	1666.0
5	2089.0
6	2796.0
7	3545.0
8	4067.0
9	4692.0
10	4971.0
11	5201.0
12	5408.0
13	5396.0
14	5675.0
15	5645.0
16	6179.0
17	6791.0
18	7812.0
19	8808.0
20	10394.0
21	12330.0
22	14748.0
23	17753.0
24	21531.0
25	26074.0
26	31007.0
27	37962.0
28	46462.0
29	57016.0
30	71150.0
31	91255.0
32	120986.0
33	172103.0
34	285525.0
35	707986.0
36	1419868.0
37	640324.0
38	112374.0
39	14090.0
40	64.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.60394123295448	18.684284831730118	16.69574546696131	29.016028468354087
2	32.89234086924144	22.268034168700012	18.833748009716015	26.00587695234254
3	30.292412935883522	19.581964141047948	19.5453856280971	30.58023729497143
4	32.41260070005001	18.18233673401227	18.242604780464447	31.162457785473276
5	32.2317347556849	18.7970624593909	18.680823798352193	30.29037898657201
6	31.380040261484265	17.951372662161667	17.253633826859588	33.41495324949448
7	33.424833762531215	17.799050229051648	17.777834553297307	30.99828145511983
8	30.69554285559642	18.545938484075254	18.611089324558687	32.14742933576964
9	31.55728803827895	17.41423195979718	18.25013733858489	32.778342663338975
10-14	31.731770607121817	18.325057272218732	18.27026959910974	31.672902521549712
15-19	31.821505693745834	18.051279571976732	18.151796153333933	31.975418580943497
20-24	31.73569025296068	18.104677717146124	18.21287890369609	31.946753126197102
25-29	31.86591492892864	18.198763631734707	18.116131591598972	31.819189847737682
30-34	31.994231063506508	18.003664051553613	17.973279624478515	32.02882526046136
35-39	31.855334106758836	18.089605982785002	18.089816125566514	31.96524378488964
40-44	31.997736955099	18.094153593892003	17.992747810570595	31.915361640438405
45-49	32.07884440432682	17.910455302238017	17.892146244747277	32.11855404868788
50-54	31.903747905902218	18.042634384762803	18.037750950243307	32.01586675909167
55-59	32.01486203591038	18.05449353123362	17.987634784521394	31.943009648334602
60-64	32.0906043015128	17.937268475821337	17.87843789045701	32.09368933220886
65-69	31.922258095048427	18.032109343347614	18.003339304508064	32.0422932570959
70-74	32.03236101618051	18.063669031834518	17.95678897839449	31.947180973590488
75-79	32.0893807233885	17.906238692241544	17.87870501924957	32.12567556512039
80-84	31.939405125250214	18.01459326816981	17.993015478587772	32.052986127992206
85-89	32.08275202323914	18.048547138944304	17.930068383167747	31.938632454648815
90-94	32.13849599598073	17.90587477163355	17.82742841451157	32.128200817874145
95-99	32.01333348272362	17.992543757680426	17.955049464856003	32.03907329473996
100-101	32.23398506724093	18.099812784140138	17.84003108678469	31.826171061834245
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.61996970130856
2	58.89821782158398
3	60.87265023085495
4	63.575058485523286
5	62.5221137422569
6	64.79499351097874
7	64.42311521765104
8	62.842972191366066
9	64.33563070161793
10-14	63.40467312867153
15-19	63.79692427468934
20-24	63.68244337915778
25-29	63.68510477666632
30-34	64.02305632396788
35-39	63.820577891648476
40-44	63.91309859553741
45-49	64.19739845301471
50-54	63.919614664993894
55-59	63.957871684244985
60-64	64.18429363372165
65-69	63.964551352144326
70-74	63.97954198977099
75-79	64.21505628850889
80-84	63.99239125324242
85-89	64.02138447788795
90-94	64.26669681385488
95-99	64.05240677746356
100-101	64.06015612907517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	5.0
2	6.5
3	8.0
4	7.0
5	7.5
6	11.5
7	19.0
8	28.5
9	34.5
10	43.5
11	69.5
12	93.5
13	112.0
14	147.5
15	175.0
16	217.5
17	275.5
18	309.0
19	345.0
20	401.0
21	459.5
22	522.5
23	619.5
24	789.5
25	1009.0
26	1259.0
27	1585.0
28	1913.5
29	2299.5
30	2714.0
31	3266.0
32	4005.0
33	4759.0
34	5473.5
35	6171.0
36	7057.5
37	8000.0
38	8968.5
39	9963.5
40	10866.5
41	11925.0
42	13080.5
43	14207.0
44	15652.5
45	17342.5
46	19483.5
47	22377.0
48	25794.5
49	29880.5
50	34685.5
51	40355.5
52	46981.0
53	54685.5
54	63309.0
55	72708.5
56	82819.5
57	93357.5
58	105431.5
59	118891.0
60	132573.0
61	146607.0
62	160998.0
63	174938.5
64	188056.0
65	199640.5
66	208010.0
67	212630.5
68	212957.0
69	208117.0
70	198540.5
71	184178.0
72	166030.5
73	145765.0
74	124053.0
75	101866.0
76	80210.0
77	61041.5
78	44714.5
79	30896.0
80	20248.0
81	12633.0
82	7528.5
83	4276.5
84	2362.0
85	1347.0
86	781.5
87	482.5
88	350.5
89	286.5
90	220.5
91	162.0
92	131.0
93	105.5
94	80.5
95	58.0
96	48.0
97	40.0
98	21.0
99	15.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12769999999999998
2	0.092775
3	0.07795
4	0.071425
5	0.076275
6	0.17754999999999999
7	0.19172499999999998
8	0.231525
9	0.20102499999999998
10-14	0.132645
15-19	0.09608499999999999
20-24	0.084275
25-29	0.07508000000000001
30-34	0.113305
35-39	0.06794499999999999
40-44	0.055009999999999996
45-49	0.04947000000000001
50-54	0.07033
55-59	0.07291
60-64	9.95E-4
65-69	1.35E-4
70-74	4.9999999999999996E-5
75-79	0.01334
80-84	0.0361
85-89	0.0031699999999999996
90-94	0.00173
95-99	0.03812
100-101	0.021825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2787616587817436
#Duplication Level	Relative count
1	100.0
2	0.18285382214585222
3	0.00601163250890473
4	0.0010019387514841219
5	5.009693757420609E-4
6	0.0010019387514841219
7	0.0
8	0.0
9	0.0
10++	0.0025048468787103044
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	249040	3.300557	4.041061	60-64
TTTTT	239045	3.2372053	3.6830244	10-14
GATCG	1281640	3.1050146	4.350651	1
CGATC	1269140	3.085232	3.4997208	1
ATCGA	711015	3.0429976	3.6794953	2
TCGAT	705360	3.0318532	3.2906044	8
>>END_MODULE