##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L003_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	64	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62778775	34.0	31.0	34.0	31.0	34.0
2	32.87662125	34.0	31.0	34.0	31.0	34.0
3	32.93096825	34.0	31.0	34.0	31.0	34.0
4	36.37768525	37.0	37.0	37.0	35.0	37.0
5	36.29487325	37.0	37.0	37.0	35.0	37.0
6	36.25520375	37.0	37.0	37.0	35.0	37.0
7	36.2373545	37.0	37.0	37.0	35.0	37.0
8	36.23373725	37.0	37.0	37.0	35.0	37.0
9	37.98866075	39.0	38.0	39.0	35.0	39.0
10-14	38.26343895	39.4	38.2	39.4	35.2	39.4
15-19	39.350539000000005	41.0	39.0	41.0	36.0	41.0
20-24	38.7260893	40.2	38.6	41.0	34.4	41.0
25-29	26.716452750000002	27.4	25.0	30.6	20.8	31.4
30-34	31.409145849999998	35.4	26.8	38.6	15.4	39.8
35-39	34.62982875	36.8	33.0	39.8	25.8	41.0
40-44	33.45262974999999	35.2	33.0	38.8	20.4	40.8
45-49	33.484228200000004	35.0	32.8	36.8	26.2	40.0
50-54	33.010043100000004	35.0	33.4	35.2	24.8	38.4
55-59	33.12316555	35.0	33.0	35.0	28.2	36.8
60-64	31.602058850000002	35.0	32.0	35.0	22.0	35.0
65-69	32.11949445	35.0	32.6	35.0	25.2	35.0
70-74	30.902942250000002	34.6	30.8	35.0	20.8	35.0
75-79	31.388801850000004	34.4	32.0	35.0	25.0	35.0
80-84	31.303725500000002	35.0	32.2	35.0	23.2	35.0
85-89	30.5859527	34.6	31.4	35.0	19.4	35.0
90-94	30.548852	34.0	31.0	35.0	20.0	35.0
95-99	30.040336500000002	34.0	31.0	35.0	12.8	35.0
100-101	28.389584499999998	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	25.0
4	71.0
5	237.0
6	781.0
7	3035.0
8	8909.0
9	9532.0
10	7263.0
11	7571.0
12	6913.0
13	7424.0
14	7867.0
15	8839.0
16	9602.0
17	10671.0
18	11393.0
19	12495.0
20	14083.0
21	16419.0
22	20592.0
23	26298.0
24	33269.0
25	42510.0
26	52928.0
27	65955.0
28	82599.0
29	105990.0
30	139883.0
31	199808.0
32	323556.0
33	543694.0
34	805468.0
35	898259.0
36	425118.0
37	80847.0
38	9901.0
39	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.56865037417657	18.830711980550618	16.916051039623813	28.684586605649
2	32.352875	21.980925000000003	19.881375000000002	25.784825
3	29.6911	19.779275	20.3674	30.162225
4	31.900125000000003	18.726025	18.9673	30.40655
5	32.08685	18.8599	18.941425000000002	30.111824999999996
6	31.266824999999997	18.018825	17.596925	33.117425
7	33.274100000000004	17.9676	17.976275	30.782025
8	30.6486	18.51145	18.7683	32.07165
9	31.4496	17.477149999999998	18.450174999999998	32.623075
10-14	31.695075	18.33359	18.378345	31.59299
15-19	31.765935	18.044345	18.245105	31.944615
20-24	32.05612808963022	18.084996647196856	18.202263505876566	31.656611757296353
25-29	41.12002825389396	16.082531572539413	15.653037331812628	27.144402841753994
30-34	33.776903097303055	18.168802766503685	17.42528488281895	30.629009253374306
35-39	32.31831932744526	18.066012458863575	18.038000849452054	31.577667364239108
40-44	32.34647802979798	18.011895674827098	17.921561061783052	31.720065233591875
45-49	32.263819999999996	18.111269999999998	17.887455	31.737454999999997
50-54	32.0282523269059	18.109397740267855	18.062361863048746	31.799988069777502
55-59	32.023559282711396	18.04874658495902	18.045556546678558	31.882137585651027
60-64	32.25543896742134	17.96963322278498	17.851083882186302	31.923843927607376
65-69	32.02616772130847	18.071753656483384	18.057228260298704	31.84485036190945
70-74	32.19317	18.175935	17.968145	31.66275
75-79	32.199355000000004	17.905135	17.88147	32.01404
80-84	32.072369975388554	18.04870216772813	18.033681591689042	31.84524626519427
85-89	32.2083848749958	18.235586870838326	17.973519520754554	31.58250873341132
90-94	32.18677627352973	17.882958586932265	17.856101123726464	32.07416401581153
95-99	32.10856981452066	17.948459479050616	17.967430075675882	31.975540630752835
100-101	32.468325	17.981975	17.8759125	31.6737875
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	64.25323697982557
2	58.1377
3	59.853325
4	62.306675
5	62.198675
6	64.38425
7	64.056125
8	62.72025
9	64.072675
10-14	63.288065
15-19	63.71055
20-24	63.71273984692657
25-29	68.26443109564796
30-34	64.40591235067737
35-39	63.89598669168437
40-44	64.06654326338985
45-49	64.001275
50-54	63.8282403966834
55-59	63.90569686836242
60-64	64.1792828950287
65-69	63.87101808321791
70-74	63.85592
75-79	64.21339499999999
80-84	63.91761624058283
85-89	63.79089360840712
90-94	64.26094028934128
95-99	64.0841104452735
100-101	64.14211250000001
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	2.5
3	4.5
4	3.0
5	3.0
6	3.5
7	3.5
8	5.5
9	5.5
10	7.0
11	18.0
12	26.5
13	26.5
14	39.5
15	57.0
16	63.5
17	77.0
18	101.0
19	134.5
20	170.5
21	226.0
22	313.0
23	414.5
24	517.0
25	689.0
26	900.5
27	1134.0
28	1449.0
29	1861.0
30	2362.5
31	2930.0
32	3498.5
33	4124.5
34	4900.0
35	5826.5
36	6745.0
37	7559.0
38	8541.5
39	9670.0
40	10638.5
41	11548.5
42	12774.0
43	14106.0
44	15543.0
45	17183.5
46	19314.0
47	21905.5
48	25141.5
49	29175.5
50	33977.5
51	40050.5
52	46674.0
53	54152.5
54	62830.0
55	72251.0
56	82499.5
57	93775.5
58	106301.5
59	119636.5
60	133779.5
61	148044.5
62	162914.0
63	177896.0
64	190751.5
65	201859.0
66	210271.5
67	214789.5
68	214802.5
69	209451.5
70	199556.0
71	185122.5
72	166911.0
73	145918.5
74	123097.5
75	100325.5
76	78676.0
77	59536.0
78	43277.5
79	29857.0
80	19577.0
81	12293.0
82	7398.0
83	4158.0
84	2293.0
85	1326.5
86	745.0
87	469.0
88	333.5
89	224.0
90	156.0
91	109.5
92	78.5
93	49.0
94	28.5
95	17.5
96	7.0
97	2.0
98	3.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0071
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0015849999999999998
25-29	1.8613149999999998
30-34	0.0905
35-39	0.041445
40-44	1.5936450000000002
45-49	0.0
50-54	0.001865
55-59	0.0012000000000000001
60-64	0.033185
65-69	0.037149999999999996
70-74	0.0
75-79	0.0
80-84	0.003835
85-89	0.01616
90-94	0.046404999999999995
95-99	0.003145
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.057489939260622486
#Duplication Level	Relative count
1	100.0
2	0.034013605442176874
3	0.0020008003201280513
4	0.0015006002400960385
5	0.0
6	0.0
7	5.002000800320128E-4
8	0.0
9	0.0
10++	0.0020008003201280513
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	234680	3.189795	3.9217274	70-74
TTTTT	226975	3.1800747	3.8424969	95-97
>>END_MODULE