##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57956525	34.0	31.0	34.0	31.0	34.0
2	32.72539475	34.0	31.0	34.0	31.0	34.0
3	32.76133925	34.0	31.0	34.0	31.0	34.0
4	36.1406555	37.0	37.0	37.0	35.0	37.0
5	36.149648	37.0	37.0	37.0	35.0	37.0
6	36.1345125	37.0	37.0	37.0	35.0	37.0
7	36.11094925	37.0	37.0	37.0	35.0	37.0
8	36.115991	37.0	37.0	37.0	35.0	37.0
9	37.87114275	39.0	39.0	39.0	35.0	39.0
10-14	38.096018650000005	39.4	38.4	39.4	35.2	39.4
15-19	39.2388044	41.0	39.4	41.0	36.0	41.0
20-24	39.00482885	41.0	39.0	41.0	35.4	41.0
25-29	38.54227225	40.2	38.2	41.0	34.6	41.0
30-34	37.782563749999994	39.8	36.6	41.0	33.2	41.0
35-39	36.6648597	38.2	35.0	40.6	32.0	41.0
40-44	35.6217149	36.0	35.0	39.6	31.0	41.0
45-49	34.6530694	35.0	34.6	38.0	30.2	40.6
50-54	33.409090049999996	34.8	33.2	35.2	28.2	39.2
55-59	33.61587485	35.0	34.2	35.0	30.0	37.4
60-64	33.29034815	35.0	34.0	35.0	29.6	35.6
65-69	33.0128878	35.0	34.0	35.0	29.0	35.0
70-74	32.73393675	35.0	33.2	35.0	29.0	35.0
75-79	32.450449799999994	35.0	33.0	35.0	27.0	35.0
80-84	32.16544555	35.0	33.0	35.0	27.0	35.0
85-89	31.8220483	35.0	33.0	35.0	25.6	35.0
90-94	31.395557149999995	35.0	33.0	35.0	24.0	35.0
95-99	30.809902649999998	35.0	32.4	35.0	21.8	35.0
100-101	29.680978875	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6666.0
3	1282.0
4	1770.0
5	2273.0
6	3068.0
7	3799.0
8	4676.0
9	5390.0
10	5743.0
11	5998.0
12	6067.0
13	6177.0
14	6098.0
15	6120.0
16	6385.0
17	7378.0
18	8399.0
19	9392.0
20	11089.0
21	12986.0
22	15594.0
23	18925.0
24	22759.0
25	27539.0
26	33213.0
27	39623.0
28	49061.0
29	59359.0
30	72837.0
31	92715.0
32	121383.0
33	170452.0
34	281190.0
35	697883.0
36	1409821.0
37	640100.0
38	112507.0
39	14220.0
40	63.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.54727219593431	18.678191979119127	16.709826539844194	29.064709285102364
2	32.81280645225849	22.262668361118337	18.834055476614566	26.090469710008605
3	30.31578715637458	19.601708720920925	19.540343465313246	30.54216065739125
4	32.372163070888206	18.244456230236562	18.22661910098867	31.15676159788656
5	32.208056128947696	18.811892580945013	18.705730263664773	30.274321026442525
6	31.404659334853857	17.943246906125417	17.243751341383554	33.40834241763717
7	33.42528977159731	17.78456527693872	17.804877743715206	30.985267207748755
8	30.66641048195749	18.54612175378448	18.622001463054865	32.16546630120317
9	31.54080637691356	17.448388870025948	18.266370453180112	32.744434299880375
10-14	31.72004339790445	18.353042622999038	18.262781224608666	31.664132754487845
15-19	31.81700673677264	18.05835141407996	18.164309741673176	31.960332107474223
20-24	31.75475713113939	18.108148131033012	18.192392941200122	31.944701796627477
25-29	31.909100825320863	18.215854356707	18.084645038909926	31.790399779062213
30-34	31.979199177123498	18.02307441840851	17.98883291914209	32.008893485325906
35-39	31.85721213906093	18.1000925931113	18.097471655864137	31.945223611963634
40-44	32.01200622444217	18.09886877694552	18.006211240380825	31.88291375823149
45-49	32.07243905917719	17.940773395696922	17.909032498238172	32.07775504688771
50-54	31.920980810597104	18.041154514978576	18.035222999476368	32.00264167494795
55-59	32.01890276218165	18.078793316767	17.989041655203483	31.913262265847862
60-64	32.08091	17.95495	17.872294999999998	32.091845
65-69	31.928219820549554	18.046085115212787	18.003140007850018	32.02255505638764
70-74	32.042105	18.07893	17.96209	31.916875
75-79	32.11065891813965	17.94675301090117	17.87979076096957	32.06279730998961
80-84	31.94886314570549	18.02757276254011	18.005871296606085	32.017692795148314
85-89	32.065445	18.079895	17.937585	31.917075
90-94	32.12383	17.9318	17.8431	32.10127
95-99	31.999475594336417	18.01251953521098	17.96410901237733	32.02389585807527
100-101	32.22070484498898	18.11900501442828	17.89976518500509	31.76052495557765
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.61198148103668
2	58.90327616226709
3	60.85794781376583
4	63.52892466877476
5	62.48237715539021
6	64.81300175249103
7	64.41055697934608
8	62.83187678316066
9	64.28524067679395
10-14	63.38417615239229
15-19	63.777338844246856
20-24	63.69945892776686
25-29	63.699500604383076
30-34	63.988092662449404
35-39	63.80243575102457
40-44	63.894919982673656
45-49	64.1501941060649
50-54	63.92362248554506
55-59	63.932165028029516
60-64	64.17275500000001
65-69	63.95077487693719
70-74	63.95898
75-79	64.17345622812927
80-84	63.96655594085381
85-89	63.98252
90-94	64.2251
95-99	64.02337145241168
100-101	63.98122980056663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	65.5
2	26.0
3	16.0
4	19.0
5	16.5
6	12.5
7	17.5
8	26.0
9	39.0
10	38.0
11	34.5
12	48.0
13	63.5
14	81.5
15	98.0
16	127.0
17	164.0
18	193.0
19	235.5
20	283.0
21	355.0
22	475.5
23	601.5
24	762.0
25	952.0
26	1173.0
27	1478.0
28	1851.0
29	2289.5
30	2829.5
31	3340.0
32	3934.0
33	4712.0
34	5546.5
35	6350.0
36	7214.0
37	8228.5
38	9187.0
39	9953.0
40	10875.0
41	11914.5
42	12916.0
43	14144.5
44	15657.0
45	17566.5
46	19830.0
47	22401.0
48	25654.0
49	29825.0
50	34750.5
51	40671.0
52	47448.5
53	54964.0
54	63128.5
55	72288.5
56	82717.0
57	93790.5
58	105549.0
59	118373.5
60	132366.5
61	147080.0
62	161449.0
63	175288.0
64	188023.0
65	199174.5
66	208004.5
67	212381.5
68	212016.5
69	207388.5
70	197812.5
71	184029.0
72	166531.5
73	145987.5
74	124427.5
75	102384.0
76	80820.5
77	61328.0
78	44649.5
79	30948.5
80	20306.0
81	12640.0
82	7416.0
83	4093.5
84	2260.0
85	1279.0
86	759.0
87	484.5
88	338.0
89	236.0
90	156.5
91	132.5
92	118.5
93	95.0
94	72.5
95	49.5
96	40.5
97	31.5
98	21.5
99	16.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0808
2	0.066
3	0.0656
4	0.068
5	0.058699999999999995
6	0.05655
7	0.061375000000000006
8	0.0721
9	0.083325
10-14	0.045865
15-19	0.04561
20-24	0.023515
25-29	0.026154999999999998
30-34	0.048185
35-39	0.03576
40-44	0.01353
45-49	0.01858
50-54	0.025549999999999996
55-59	0.024135
60-64	0.0
65-69	2.5E-4
70-74	0.0
75-79	0.0033599999999999997
80-84	0.006755000000000001
85-89	0.0
90-94	0.0
95-99	0.00108
100-101	0.0010625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.28727018385290864
#Duplication Level	Relative count
1	100.0
2	0.18688779216875015
3	0.009519753488488615
4	0.0010020793145777487
5	0.0010020793145777487
6	5.010396572888744E-4
7	0.0010020793145777487
8	0.0015031189718666233
9	5.010396572888744E-4
10++	0.006012475887466493
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	263660	3.476182	4.116747	60-64
TTTTT	238755	3.2355795	3.4920197	9
GATCG	1277870	3.0924423	4.32138	1
CGATC	1269715	3.0861714	3.4500246	6
ATCGA	710835	3.0368772	3.7912736	2
TCGAT	706445	3.034765	3.2853212	5
>>END_MODULE