##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62049025	34.0	31.0	34.0	31.0	34.0
2	32.79756675	34.0	31.0	34.0	31.0	34.0
3	32.84434875	34.0	31.0	34.0	31.0	34.0
4	36.18645475	37.0	37.0	37.0	35.0	37.0
5	36.208346	37.0	37.0	37.0	35.0	37.0
6	36.2042785	37.0	37.0	37.0	35.0	37.0
7	36.18936375	37.0	37.0	37.0	35.0	37.0
8	36.14719725	37.0	37.0	37.0	35.0	37.0
9	37.90787425	39.0	39.0	39.0	35.0	39.0
10-14	38.1806004	39.4	38.8	39.4	35.4	39.4
15-19	39.27478515	41.0	39.6	41.0	36.2	41.0
20-24	39.0628847	41.0	39.0	41.0	35.6	41.0
25-29	38.58410335	40.2	38.2	41.0	35.0	41.0
30-34	37.8704427	39.8	36.8	41.0	33.4	41.0
35-39	36.71501425	38.2	35.0	40.6	32.4	41.0
40-44	35.6915479	36.0	35.0	39.6	31.0	41.0
45-49	34.7117009	35.0	34.8	38.0	30.6	40.6
50-54	33.43024405	34.8	33.2	35.2	28.2	39.2
55-59	33.693528	35.0	34.6	35.0	30.2	37.4
60-64	33.3679064	35.0	34.0	35.0	30.0	35.6
65-69	33.08070735	35.0	34.0	35.0	29.2	35.0
70-74	32.80386405	35.0	33.4	35.0	29.0	35.0
75-79	32.5022465	35.0	33.0	35.0	27.8	35.0
80-84	32.18299105	35.0	33.0	35.0	27.0	35.0
85-89	31.8349842	35.0	33.0	35.0	25.8	35.0
90-94	31.367696549999998	35.0	33.0	35.0	24.0	35.0
95-99	30.7649592	35.0	32.0	35.0	21.2	35.0
100-101	29.6054565	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6896.0
3	1240.0
4	1826.0
5	2212.0
6	2969.0
7	3602.0
8	4303.0
9	4967.0
10	5132.0
11	5578.0
12	5611.0
13	5709.0
14	5741.0
15	5873.0
16	6377.0
17	7171.0
18	7812.0
19	9103.0
20	10472.0
21	12337.0
22	14822.0
23	17798.0
24	21722.0
25	26012.0
26	31632.0
27	38409.0
28	46542.0
29	57508.0
30	71150.0
31	91634.0
32	121412.0
33	172990.0
34	293650.0
35	725117.0
36	1404355.0
37	626087.0
38	110490.0
39	13672.0
40	67.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.605270349951795	18.719873381859607	16.69556486126364	28.979291406924958
2	32.79790191765327	22.30764033303053	18.84725590148222	26.04720184783397
3	30.275640473907657	19.645745219589624	19.555546436985455	30.523067869517266
4	32.340001410968625	18.245375450246595	18.239296276894088	31.17532686189069
5	32.2540533347208	18.79735227491035	18.67943435418801	30.269160036180843
6	31.348584801125778	17.938125351117094	17.266982061324367	33.44630778643276
7	33.41603147984392	17.764537733625392	17.831722158248972	30.987708628281712
8	30.707331432324626	18.544747128462266	18.628350127356903	32.119571311856205
9	31.531877006087818	17.431671926122153	18.30861218209539	32.72783888569464
10-14	31.714178715393253	18.34938457270072	18.276536422124266	31.659900289781756
15-19	31.79407715324871	18.084429349802907	18.15649591200875	31.96499758493963
20-24	31.733721774274613	18.142918286268078	18.206818794921528	31.916541144535778
25-29	31.91378246783612	18.213616713364196	18.09547501594678	31.777125802852908
30-34	31.972652212897057	18.02771296319085	17.982931369506375	32.016703454405715
35-39	31.87822973829992	18.113907855803877	18.071764960077783	31.93609744581842
40-44	31.996348950843462	18.11616605432446	18.00642154175012	31.88106345308196
45-49	32.0664252865545	17.97805276208207	17.896417578092866	32.059104373270564
50-54	31.918041869023178	18.02870521159402	18.042714267916274	32.01053865146653
55-59	32.00435576436623	18.078252525109058	18.00093540709913	31.916456303425576
60-64	32.068585	17.92481	17.890715	32.11589
65-69	31.916867988496545	18.039674296387766	18.023209214885615	32.020248500230075
70-74	32.05213	18.07638	17.962895	31.908595000000002
75-79	32.1080843075119	17.930729164765577	17.868417603860976	32.09276892386154
80-84	31.944571186075155	18.011999422574853	18.005879156037246	32.03755023531275
85-89	32.088955000000006	18.05792	17.951575000000002	31.90155
90-94	32.129039999999996	17.89199	17.864525	32.114445
95-99	32.00271524371542	18.00070481134746	17.963254208392755	32.03332573654436
100-101	32.21748707898414	18.10634771701844	17.892141237272426	31.784023966724995
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.58456175687675
2	58.84510376548725
3	60.79870834342492
4	63.515328272859314
5	62.523213370901644
6	64.79489258755854
7	64.40374010812565
8	62.826902744180835
9	64.25971589178245
10-14	63.37407900517501
15-19	63.759074738188346
20-24	63.6502629188104
25-29	63.69090827068903
30-34	63.989355667302775
35-39	63.81432718411833
40-44	63.87741240392542
45-49	64.12552965982506
50-54	63.92858052048971
55-59	63.92081206779181
60-64	64.18447499999999
65-69	63.937116488726616
70-74	63.960725
75-79	64.20085323137344
80-84	63.9821214213879
85-89	63.990505
90-94	64.243485
95-99	64.03604098025978
100-101	64.00151104570914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	78.0
2	35.0
3	32.5
4	36.5
5	34.0
6	38.0
7	37.0
8	31.5
9	34.0
10	47.0
11	57.0
12	65.5
13	83.0
14	94.0
15	112.5
16	135.0
17	156.0
18	188.5
19	230.0
20	306.5
21	374.5
22	457.0
23	584.5
24	755.0
25	965.0
26	1196.0
27	1481.5
28	1836.0
29	2248.0
30	2728.5
31	3319.0
32	4040.5
33	4833.0
34	5612.0
35	6426.0
36	7291.5
37	8186.5
38	9134.5
39	9927.5
40	10800.5
41	11862.0
42	13034.5
43	14338.5
44	15699.5
45	17377.5
46	19554.5
47	22386.0
48	25787.5
49	29800.5
50	34775.5
51	40621.0
52	46990.0
53	54395.5
54	63158.0
55	72563.5
56	82446.0
57	93711.5
58	105853.0
59	118724.0
60	132804.5
61	146946.5
62	161596.0
63	176053.5
64	188684.0
65	199437.5
66	207519.0
67	211870.5
68	211906.5
69	207491.0
70	198454.0
71	184698.0
72	166544.5
73	145609.0
74	123797.0
75	101894.0
76	80450.5
77	60929.5
78	44295.0
79	30653.0
80	20181.5
81	12613.0
82	7447.5
83	4169.5
84	2363.5
85	1393.5
86	842.0
87	557.5
88	395.0
89	299.0
90	231.0
91	176.0
92	136.0
93	105.5
94	86.5
95	69.0
96	52.5
97	35.5
98	23.0
99	18.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1091
2	0.09924999999999999
3	0.0818
4	0.06865
5	0.0576
6	0.05115
7	0.08845
8	0.1232
9	0.124325
10-14	0.05237
15-19	0.06461
20-24	0.02427
25-29	0.026830000000000003
30-34	0.04822
35-39	0.030600000000000002
40-44	0.01778
45-49	0.012475
50-54	0.028955
55-59	0.02214
60-64	0.0
65-69	4.95E-4
70-74	0.0
75-79	0.002505
80-84	0.004355
85-89	0.0
90-94	0.0
95-99	0.0016099999999999999
100-101	0.003025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2323710340760881
#Duplication Level	Relative count
1	100.0
2	0.16328902156817568
3	0.006511525399957926
4	0.004007092553820262
5	0.0010017731384550654
6	0.0
7	5.008865692275327E-4
8	0.0
9	5.008865692275327E-4
10++	0.0015026597076825982
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	270875	3.556643	4.239551	60-64
TTTTT	243750	3.290553	3.6127665	90-94
GATCG	1279930	3.0964992	4.3844647	1
CGATC	1265910	3.076879	3.530387	6
TCGAT	705945	3.0283508	3.4236443	5
ATCGA	709840	3.028205	3.6644638	2
>>END_MODULE