##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57521525	34.0	31.0	34.0	31.0	34.0
2	32.74356575	34.0	31.0	34.0	31.0	34.0
3	32.78972825	34.0	31.0	34.0	31.0	34.0
4	36.15704475	37.0	37.0	37.0	35.0	37.0
5	36.1600775	37.0	37.0	37.0	35.0	37.0
6	36.1519135	37.0	37.0	37.0	35.0	37.0
7	36.138511	37.0	37.0	37.0	35.0	37.0
8	36.125198	37.0	37.0	37.0	35.0	37.0
9	37.87728075	39.0	39.0	39.0	35.0	39.0
10-14	38.096778799999996	39.4	38.6	39.4	35.2	39.4
15-19	39.05594355000001	41.0	39.4	41.0	35.6	41.0
20-24	38.493172050000005	40.4	38.4	41.0	34.0	41.0
25-29	38.47828695	40.0	38.0	41.0	34.8	41.0
30-34	37.773355200000005	39.8	36.4	41.0	33.2	41.0
35-39	36.58876195	38.2	35.0	40.6	31.8	41.0
40-44	35.58268705	35.8	35.0	39.6	31.0	41.0
45-49	34.653925900000004	35.0	34.6	38.0	30.4	40.6
50-54	33.403601550000005	34.8	33.0	35.2	28.2	39.2
55-59	33.60348325	35.0	34.0	35.0	30.0	37.4
60-64	33.269118750000004	35.0	34.0	35.0	29.6	35.6
65-69	32.959092250000005	35.0	34.0	35.0	29.0	35.0
70-74	32.6823282	35.0	33.0	35.0	28.6	35.0
75-79	32.24402385	35.0	33.0	35.0	27.0	35.0
80-84	31.995805099999995	35.0	33.0	35.0	27.0	35.0
85-89	31.65426395	35.0	33.0	35.0	24.4	35.0
90-94	30.922037700000004	35.0	32.2	35.0	21.4	35.0
95-99	30.37850235	35.0	31.0	35.0	16.0	35.0
100-101	28.690813125	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7830.0
3	1426.0
4	2062.0
5	2449.0
6	3227.0
7	4049.0
8	4714.0
9	5233.0
10	5711.0
11	5837.0
12	6240.0
13	6101.0
14	6257.0
15	6348.0
16	6766.0
17	7808.0
18	8530.0
19	9855.0
20	11295.0
21	13666.0
22	15945.0
23	19546.0
24	23230.0
25	27937.0
26	33745.0
27	40892.0
28	49812.0
29	60742.0
30	76056.0
31	97781.0
32	129693.0
33	187332.0
34	324617.0
35	774624.0
36	1331248.0
37	568330.0
38	101172.0
39	11839.0
40	55.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.534977466199294	18.71489734601903	16.72308462694041	29.02704056084126
2	32.815834755578294	22.33358482329034	18.840644648695896	26.009935772435465
3	30.264356836306877	19.633599768159407	19.559923310415385	30.54212008511833
4	32.31938221480138	18.28568010826957	18.24030919290322	31.15462848402583
5	32.2020160059259	18.84920495893414	18.748154413641576	30.200624621498388
6	31.361923339188348	17.954736131699267	17.276098290172833	33.40724223893955
7	33.403130461241574	17.813181247660363	17.803347259395085	30.98034103170298
8	30.647169735286635	18.608704837046805	18.693706265000714	32.05041916266585
9	31.464737658067694	17.463798550390187	18.30278813860949	32.768675652932636
10-14	31.727905015104046	18.365175740268423	18.27373596784474	31.633183276782795
15-19	32.029787089259194	18.073355936002688	18.093124932452177	31.803732042285937
20-24	31.82212989415969	18.23312338936291	18.124802644514734	31.81994407196267
25-29	31.871411560046752	18.219545062330134	18.124489402199234	31.78455397542388
30-34	31.960649149751063	18.027856909619306	17.98568828042794	32.0258056602017
35-39	31.89540745087586	18.1453106013842	18.082856747134567	31.876425200605375
40-44	31.980000789268786	18.12810352565566	18.00630204616182	31.885593638913733
45-49	32.059805283170746	17.95331692923427	17.917220843434205	32.06965694416078
50-54	31.889474778098943	18.051357442467296	18.059595962374924	31.999571817058836
55-59	31.996405368659037	18.09933992889264	17.999155610754464	31.905099091693863
60-64	32.07474424484655	17.953180771908464	17.880315728189437	32.09175925505555
65-69	31.917205855110247	18.039686569451497	18.023546541206446	32.019561034231806
70-74	32.036815000000004	18.079245	17.969045	31.914894999999998
75-79	32.15009628268482	17.94973659331555	17.862671904882077	32.03749521911755
80-84	31.950267972434837	18.037708408767436	18.016131401026865	31.995892217770866
85-89	32.06162160308108	18.070425903521294	17.944485897224293	31.923466596173327
90-94	32.20208194562125	17.982498552746094	17.83121814428899	31.984201357343668
95-99	32.018348596328785	18.002792551635554	17.973510961645218	32.00534789039045
100-101	32.33542748497772	18.231421176018756	17.90389639225162	31.529254946751905
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.56201802704055
2	58.82577052801376
3	60.806476921425215
4	63.47401069882721
5	62.40264062742429
6	64.7691655781279
7	64.38347149294455
8	62.697588897952485
9	64.23341331100032
10-14	63.36108829188684
15-19	63.83351913154514
20-24	63.64207396612236
25-29	63.65596553547063
30-34	63.986454809952754
35-39	63.77183265148123
40-44	63.86559442818252
45-49	64.12946222733153
50-54	63.88904659515779
55-59	63.901504460352896
60-64	64.16650349990209
65-69	63.93676688934205
70-74	63.95170999999999
75-79	64.18759150180237
80-84	63.9461601902057
85-89	63.98508819925441
90-94	64.18628330296492
95-99	64.02369648671923
100-101	63.864682431729626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	73.0
2	32.5
3	29.0
4	32.0
5	30.5
6	21.5
7	27.0
8	43.5
9	45.0
10	47.5
11	58.0
12	64.0
13	79.5
14	104.5
15	115.5
16	137.0
17	179.0
18	230.5
19	280.0
20	327.5
21	385.0
22	497.0
23	624.0
24	777.5
25	986.5
26	1216.5
27	1485.5
28	1828.5
29	2287.5
30	2804.5
31	3368.5
32	4038.5
33	4751.0
34	5579.5
35	6368.5
36	7222.0
37	8174.5
38	9031.5
39	9954.5
40	10966.5
41	11910.5
42	12965.0
43	14341.5
44	15838.5
45	17475.0
46	19663.5
47	22534.5
48	25891.5
49	29916.0
50	34871.0
51	40494.0
52	47063.5
53	54816.0
54	63360.5
55	72817.0
56	83112.5
57	94158.5
58	106362.0
59	119399.0
60	133044.5
61	147381.5
62	161579.5
63	175487.5
64	188478.0
65	199619.5
66	207530.0
67	211851.0
68	212617.0
69	207971.0
70	198370.0
71	184253.5
72	166170.0
73	145324.5
74	122847.0
75	100617.0
76	79280.5
77	60197.0
78	43750.5
79	30188.5
80	20146.0
81	12725.5
82	7588.0
83	4377.5
84	2503.0
85	1437.5
86	886.5
87	618.0
88	418.0
89	330.0
90	254.5
91	204.0
92	168.5
93	111.0
94	85.0
95	71.0
96	48.0
97	40.0
98	36.0
99	25.0
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.12855
3	0.103775
4	0.10120000000000001
5	0.0995
6	0.05714999999999999
7	0.0914
8	0.11932500000000001
9	0.11795
10-14	0.0599
15-19	0.0708
20-24	0.037615
25-29	0.037515
30-34	0.056035
35-39	0.038195
40-44	0.034055
45-49	0.01686
50-54	0.042725
55-59	0.034255
60-64	5.9999999999999995E-5
65-69	1.75E-4
70-74	0.0
75-79	0.005385
80-84	0.009304999999999999
85-89	4.9999999999999996E-6
90-94	2.7E-4
95-99	0.00543
100-101	0.015200000000000002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2148925537231378
#Duplication Level	Relative count
1	100.0
2	0.15525617268493014
3	0.0060099163619972956
4	0.0015024790904993239
5	5.008263634997747E-4
6	0.0010016527269995493
7	0.0
8	0.0
9	0.0
10++	0.0010016527269995493
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	270740	3.5430994	4.4667625	1
TTTTT	247210	3.340985	3.6237192	95-97
GATCG	1274320	3.0807762	4.337133	1
CGATC	1260720	3.0664206	3.3986819	1
ATCGA	710340	3.0278893	3.8382907	2
TCGAT	703000	3.015953	3.358678	8
>>END_MODULE