##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52294425	34.0	31.0	34.0	31.0	34.0
2	32.694336	34.0	31.0	34.0	31.0	34.0
3	32.725823	34.0	31.0	34.0	31.0	34.0
4	36.0986115	37.0	37.0	37.0	35.0	37.0
5	36.093102	37.0	37.0	37.0	35.0	37.0
6	36.1005965	37.0	37.0	37.0	35.0	37.0
7	36.10783725	37.0	37.0	37.0	35.0	37.0
8	36.10901225	37.0	37.0	37.0	35.0	37.0
9	37.8393485	39.0	39.0	39.0	35.0	39.0
10-14	38.1092474	39.4	38.4	39.4	35.2	39.4
15-19	39.167402100000004	41.0	39.0	41.0	36.0	41.0
20-24	38.90019245	41.0	39.0	41.0	35.2	41.0
25-29	38.5124747	40.0	38.2	41.0	34.8	41.0
30-34	37.7135431	39.8	36.4	41.0	33.0	41.0
35-39	36.599789099999995	38.2	35.0	40.6	31.6	41.0
40-44	35.53753345	35.8	35.0	39.6	31.0	41.0
45-49	34.5922949	35.0	34.6	38.0	30.2	40.6
50-54	33.327425	34.8	33.0	35.2	28.0	39.2
55-59	33.5442457	35.0	34.0	35.0	30.0	37.4
60-64	33.198061599999996	35.0	34.0	35.0	29.4	35.6
65-69	32.907173	35.0	33.8	35.0	29.0	35.0
70-74	32.6329732	35.0	33.0	35.0	28.2	35.0
75-79	32.296932899999995	35.0	33.0	35.0	27.0	35.0
80-84	31.96481005	35.0	33.0	35.0	26.2	35.0
85-89	31.657303050000003	35.0	33.0	35.0	24.4	35.0
90-94	31.17427645	35.0	32.8	35.0	23.6	35.0
95-99	30.5387269	35.0	31.6	35.0	19.2	35.0
100-101	29.309138	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7236.0
3	1377.0
4	1955.0
5	2674.0
6	3415.0
7	4382.0
8	5128.0
9	5929.0
10	6013.0
11	6272.0
12	6651.0
13	6610.0
14	6514.0
15	6547.0
16	7159.0
17	7945.0
18	8912.0
19	9950.0
20	11435.0
21	13559.0
22	16252.0
23	19330.0
24	23358.0
25	28076.0
26	34023.0
27	41062.0
28	49857.0
29	61443.0
30	75655.0
31	96169.0
32	126420.0
33	178492.0
34	295763.0
35	707858.0
36	1371850.0
37	621755.0
38	109296.0
39	13616.0
40	62.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.54536322175083	18.714122159497386	16.721238027773385	29.0192765909784
2	32.79426539385716	22.29073148640528	18.872546134168005	26.042456985569558
3	30.3101032592419	19.60878880485115	19.51981798167196	30.561289954234994
4	32.336502952544876	18.286341749806137	18.24348626720982	31.133669030439165
5	32.23465621136123	18.820025730792583	18.721305857763223	30.22401220008296
6	31.308199244907076	17.983144693469182	17.288717267261784	33.41993879436196
7	33.37449745262625	17.814385334169618	17.85387387874041	30.95724333446372
8	30.699890003636533	18.584508370278073	18.637705792991746	32.07789583309365
9	31.51852635782148	17.48894282996502	18.283472864586383	32.709057947627116
10-14	31.70240391938747	18.371723704546884	18.276547496038575	31.64932488002707
15-19	31.842662771267527	18.094129333938653	18.157799768210218	31.905408126583605
20-24	31.755819172952876	18.142042877987542	18.207291358994002	31.89484659006558
25-29	31.86693724235401	18.230483605207365	18.11022255893626	31.79235659350236
30-34	31.972834871909456	18.030018124654596	17.9955421241067	32.00160487932925
35-39	31.848668343100528	18.12383610148925	18.10454071172238	31.922954843687844
40-44	31.988388962264125	18.11488887338884	18.02211357186911	31.874608592477927
45-49	32.04450165785215	17.966688356694863	17.920708296920786	32.068101688532195
50-54	31.905188965150057	18.059469978152347	18.0340242173241	32.001316839373494
55-59	32.01688512663845	18.078290587179406	18.01124008430063	31.893584201881513
60-64	32.075355	17.91908	17.902065	32.103500000000004
65-69	31.918355	18.04554	18.035105	32.001000000000005
70-74	32.027865	18.096625	17.970135000000003	31.905375000000003
75-79	32.100345000000004	17.944415	17.868015	32.087225000000004
80-84	31.934563193456317	18.04144180414418	18.00327680032768	32.02071820207182
85-89	32.06133	18.071225	17.94719	31.920254999999997
90-94	32.140215	17.925575	17.84628	32.08793
95-99	32.029055	17.987995	17.95601	32.026939999999996
100-101	32.234511493347696	18.139004181535544	17.892089582761454	31.73439474235531
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.56463981272923
2	58.83672237942672
3	60.871393213476885
4	63.47017198298405
5	62.458668411444194
6	64.72813803926904
7	64.33174078708997
8	62.77778583673018
9	64.2275843054486
10-14	63.351728799414545
15-19	63.74807089785113
20-24	63.65066576301845
25-29	63.65929383585637
30-34	63.9744397512387
35-39	63.771623186788375
40-44	63.86299755474205
45-49	64.11260334638435
50-54	63.90650580452355
55-59	63.91046932851996
60-64	64.178855
65-69	63.919355
70-74	63.933240000000005
75-79	64.18757000000001
80-84	63.95528139552814
85-89	63.981584999999995
90-94	64.228145
95-99	64.055995
100-101	63.968906235703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	135.0
1	89.5
2	35.5
3	32.5
4	36.0
5	33.0
6	36.5
7	42.0
8	48.0
9	49.0
10	56.0
11	68.5
12	73.5
13	74.0
14	90.5
15	113.0
16	132.0
17	162.5
18	187.5
19	228.0
20	283.5
21	369.5
22	454.0
23	537.0
24	702.5
25	922.5
26	1187.0
27	1475.5
28	1792.5
29	2228.0
30	2804.5
31	3388.5
32	4060.5
33	4782.0
34	5568.5
35	6483.0
36	7311.5
37	8143.5
38	9028.5
39	10034.5
40	10915.0
41	11879.5
42	13009.5
43	14278.0
44	15784.0
45	17575.5
46	19857.5
47	22345.5
48	25545.5
49	29779.5
50	34744.0
51	40658.0
52	47326.0
53	54656.5
54	63295.5
55	72905.5
56	82908.5
57	93776.5
58	105962.5
59	118979.0
60	132689.0
61	146834.5
62	161165.0
63	175603.5
64	189002.5
65	199811.0
66	207966.5
67	212750.0
68	212254.0
69	207386.5
70	198315.5
71	184262.0
72	165980.5
73	145385.0
74	123415.5
75	100824.5
76	79825.5
77	60791.0
78	44027.5
79	30364.5
80	20068.0
81	12730.5
82	7619.5
83	4327.0
84	2439.0
85	1461.5
86	895.0
87	619.0
88	444.0
89	316.5
90	262.0
91	196.0
92	148.0
93	117.0
94	88.5
95	71.0
96	54.5
97	41.5
98	29.0
99	12.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23002500000000003
2	0.19192499999999998
3	0.16390000000000002
4	0.1878
5	0.19740000000000002
6	0.025549999999999996
7	0.0343
8	0.04215
9	0.07615
10-14	0.01703
15-19	0.008535000000000001
20-24	0.005335
25-29	8.7E-4
30-34	0.017405
35-39	0.00202
40-44	3.25E-4
45-49	1.3000000000000002E-4
50-54	0.00299
55-59	7.5E-4
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	9.999999999999999E-6
85-89	0.0
90-94	0.0
95-99	0.0
100-101	8.5E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.21739890950707486
#Duplication Level	Relative count
1	100.0
2	0.16127578158651293
3	0.004507708180989492
4	0.003005138787326328
5	5.008564645543881E-4
6	0.0
7	0.0010017129291087761
8	0.0
9	0.0
10++	0.0010017129291087761
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	276150	3.6259496	4.4291	1
TTTTT	250465	3.3781915	3.7736642	95-97
GATCG	1279520	3.0947874	4.352106	1
CGATC	1267415	3.0800853	3.5239618	6
ATCGA	709640	3.026799	3.7905617	2
TCGAT	705775	3.026522	3.4719558	5
>>END_MODULE