##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7118435	34.0	31.0	34.0	31.0	34.0
2	32.8895485	34.0	33.0	34.0	31.0	34.0
3	32.91175875	34.0	33.0	34.0	31.0	34.0
4	36.27595975	37.0	37.0	37.0	35.0	37.0
5	36.2722955	37.0	37.0	37.0	35.0	37.0
6	36.2485875	37.0	37.0	37.0	35.0	37.0
7	36.240054	37.0	37.0	37.0	35.0	37.0
8	36.21572225	37.0	37.0	37.0	35.0	37.0
9	37.997198	39.0	39.0	39.0	37.0	39.0
10-14	38.30577995	39.4	39.2	39.4	37.0	39.4
15-19	39.47191395	41.0	40.0	41.0	37.0	41.0
20-24	39.24006115	41.0	39.4	41.0	36.2	41.0
25-29	38.800726	40.4	38.6	41.0	35.0	41.0
30-34	38.025428399999996	40.0	37.0	41.0	33.8	41.0
35-39	36.936238599999996	38.4	35.0	40.6	32.8	41.0
40-44	35.8738423	36.0	35.0	39.8	32.0	41.0
45-49	34.94166945	35.0	35.0	38.0	31.0	40.8
50-54	33.7039963	34.8	33.6	35.4	29.4	39.2
55-59	33.896757550000004	35.0	35.0	35.0	31.0	37.6
60-64	33.55836825	35.0	34.8	35.0	31.0	35.6
65-69	33.2510003	35.0	34.0	35.0	30.0	35.0
70-74	32.97268645	35.0	34.0	35.0	29.2	35.0
75-79	32.712696199999996	35.0	33.2	35.0	29.0	35.0
80-84	32.395927050000004	35.0	33.0	35.0	27.0	35.0
85-89	32.06631505	35.0	33.0	35.0	27.0	35.0
90-94	31.675962600000002	35.0	33.0	35.0	25.0	35.0
95-99	31.0789175	35.0	33.0	35.0	23.4	35.0
100-101	29.8930675	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6283.0
3	1072.0
4	1530.0
5	1927.0
6	2507.0
7	3070.0
8	3722.0
9	4312.0
10	4559.0
11	4821.0
12	4928.0
13	5019.0
14	5088.0
15	5316.0
16	5739.0
17	6427.0
18	7155.0
19	8233.0
20	9794.0
21	11182.0
22	13343.0
23	16135.0
24	19312.0
25	23697.0
26	28177.0
27	34573.0
28	42339.0
29	52190.0
30	65134.0
31	83728.0
32	111249.0
33	160524.0
34	272682.0
35	703200.0
36	1467669.0
37	671163.0
38	117388.0
39	14746.0
40	67.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.5908193404359	18.67914393642502	16.69694583180477	29.033090891334318
2	32.854782129272145	22.254620607291614	18.85857967270651	26.03201759072973
3	30.275631899432724	19.59178585802762	19.531214019827516	30.60136822271214
4	32.45825992953235	18.218614870275466	18.178876721652788	31.1442484785394
5	32.20511678475049	18.79628320599131	18.717763618006458	30.280836391251746
6	31.323035136986576	17.936598390372684	17.271820905926134	33.46854556671461
7	33.40854950156246	17.76958811072027	17.777526250325852	31.044336137391415
8	30.694688325839458	18.559447916536204	18.613554099921096	32.132309657703246
9	31.572129152357192	17.441133793925008	18.215529953028266	32.77120710068954
10-14	31.758537265557056	18.29537872321552	18.259906953433013	31.686177057794406
15-19	31.820432995256702	18.028932411665892	18.135831475027906	32.01480311804949
20-24	31.763148829450344	18.08959884835527	18.191067129349623	31.95618519284476
25-29	31.895495525606236	18.18513727640102	18.092738904977978	31.826628293014764
30-34	32.000067468781054	17.987552410304687	17.992597553575994	32.019782567338275
35-39	31.860289013219862	18.074506022046858	18.0875495166227	31.977655448110582
40-44	32.005874960559154	18.07704335602045	17.998821926650127	31.918259756770272
45-49	32.07810964671293	17.905561864813922	17.886990053602158	32.12933843487099
50-54	31.900004221789196	18.027134899770523	18.035828584153965	32.03703229428632
55-59	32.02774682324447	18.059287163732552	17.986875566911518	31.926090446111466
60-64	32.09671949154436	17.918288115760028	17.86790775554045	32.11708463715516
65-69	31.940890896559072	18.031501555127722	18.000761501332626	32.02684604698058
70-74	32.05417	18.05622	17.945035	31.944574999999997
75-79	32.100887340113346	17.903285513118686	17.85684658206317	32.1389805647048
80-84	31.951788977913147	18.016803686927602	17.990959708247082	32.04044762691216
85-89	32.06968375534905	18.03263573476504	17.94500903042564	31.952671479460275
90-94	32.1600953207864	17.874397463779076	17.825302058741986	32.140205156692545
95-99	32.02398693687385	17.986982180522517	17.938154980951943	32.0508759016517
100-101	32.24277111220294	18.08634034312604	17.858869006764756	31.81201953790626
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.62391023177021
2	58.88679972000187
3	60.877000122144864
4	63.60250840807174
5	62.485953176002226
6	64.79158070370119
7	64.45288563895389
8	62.8269979835427
9	64.34333625304673
10-14	63.44471432335146
15-19	63.8352361133062
20-24	63.7193340222951
25-29	63.722123818621
30-34	64.01985003611932
35-39	63.837944461330444
40-44	63.92413471732943
45-49	64.20744808158392
50-54	63.937036516075516
55-59	63.95383726935593
60-64	64.21380412869952
65-69	63.967736943539656
70-74	63.998745
75-79	64.23986790481814
80-84	63.992236604825315
85-89	64.02235523480931
90-94	64.30030047747894
95-99	64.07486283852553
100-101	64.05479065010921
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	10.5
2	8.0
3	7.0
4	8.5
5	11.5
6	12.0
7	11.0
8	18.5
9	28.5
10	38.0
11	55.0
12	68.0
13	80.5
14	103.5
15	134.0
16	161.0
17	183.5
18	229.0
19	292.5
20	333.5
21	374.0
22	485.0
23	634.5
24	745.5
25	932.5
26	1216.0
27	1484.5
28	1809.0
29	2277.5
30	2764.5
31	3299.0
32	3982.5
33	4679.0
34	5422.5
35	6283.5
36	7148.5
37	8085.5
38	9008.0
39	9797.5
40	10721.0
41	11786.5
42	12872.0
43	14072.0
44	15652.5
45	17351.5
46	19423.5
47	22192.5
48	25566.0
49	29644.0
50	34663.5
51	40593.5
52	46937.5
53	54358.5
54	62850.0
55	72143.5
56	82732.5
57	94031.0
58	105952.0
59	118945.5
60	132035.0
61	146489.5
62	161834.0
63	175902.5
64	188862.0
65	199408.5
66	207330.5
67	212041.0
68	212187.0
69	207408.5
70	198552.0
71	184849.5
72	166642.5
73	146213.5
74	124395.5
75	102204.5
76	80753.0
77	61248.0
78	44447.5
79	30871.5
80	20419.0
81	12770.0
82	7514.0
83	4233.5
84	2372.0
85	1349.0
86	809.5
87	572.0
88	413.0
89	287.0
90	210.5
91	156.0
92	124.5
93	100.0
94	82.0
95	62.0
96	41.5
97	29.5
98	23.5
99	18.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.165125
2	0.106475
3	0.1186
4	0.096
5	0.088625
6	0.1508
7	0.165525
8	0.19624999999999998
9	0.189975
10-14	0.11775500000000001
15-19	0.09267
20-24	0.05251
25-29	0.06858500000000001
30-34	0.10194500000000001
35-39	0.065125
40-44	0.04018
45-49	0.036675
50-54	0.04238
55-59	0.05054
60-64	7.15E-4
65-69	1.75E-4
70-74	0.0
75-79	0.008465
80-84	0.015395
85-89	0.001945
90-94	8.250000000000001E-4
95-99	0.014745000000000001
100-101	0.014875000000000001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2448530881471117
#Duplication Level	Relative count
1	100.0
2	0.1733206431899013
3	0.005009267144216801
4	0.0020037068576867203
5	5.009267144216801E-4
6	0.0015027801432650402
7	5.009267144216801E-4
8	0.0
9	5.009267144216801E-4
10++	0.0020037068576867203
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	249515	3.305965	4.187427	1
TTTTT	238170	3.2295785	3.5246031	10-14
GATCG	1275450	3.0879166	4.284147	1
CGATC	1267265	3.081872	3.4252007	6
ATCGA	712630	3.0496607	3.784747	2
TCGAT	705370	3.032605	3.3680158	5
>>END_MODULE