##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65813525	34.0	31.0	34.0	31.0	34.0
2	32.81975275	34.0	31.0	34.0	31.0	34.0
3	32.86303125	34.0	33.0	34.0	31.0	34.0
4	36.23551075	37.0	37.0	37.0	35.0	37.0
5	36.23655775	37.0	37.0	37.0	35.0	37.0
6	36.2167975	37.0	37.0	37.0	35.0	37.0
7	36.20947575	37.0	37.0	37.0	35.0	37.0
8	36.18808025	37.0	37.0	37.0	35.0	37.0
9	37.958626	39.0	39.0	39.0	37.0	39.0
10-14	38.25101945	39.4	39.2	39.4	37.0	39.4
15-19	39.4013997	41.0	40.0	41.0	37.0	41.0
20-24	39.164370299999995	41.0	39.2	41.0	35.8	41.0
25-29	38.727035	40.4	38.4	41.0	35.0	41.0
30-34	37.96265705	40.0	36.8	41.0	33.6	41.0
35-39	36.87262439999999	38.4	35.0	40.6	32.6	41.0
40-44	35.77733685	36.0	35.0	39.6	31.2	41.0
45-49	34.8489917	35.0	35.0	38.0	31.0	40.8
50-54	33.587769200000004	34.8	33.4	35.4	28.6	39.2
55-59	33.8067415	35.0	35.0	35.0	31.0	37.6
60-64	33.46391475	35.0	34.4	35.0	30.4	35.6
65-69	33.1363211	35.0	34.0	35.0	29.6	35.0
70-74	32.8670504	35.0	33.8	35.0	29.0	35.0
75-79	32.57963205	35.0	33.0	35.0	28.2	35.0
80-84	32.25993175000001	35.0	33.0	35.0	27.0	35.0
85-89	31.8974428	35.0	33.0	35.0	26.2	35.0
90-94	31.4919288	35.0	33.0	35.0	24.2	35.0
95-99	30.867485549999998	35.0	32.4	35.0	22.0	35.0
100-101	29.678654	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5759.0
3	1175.0
4	1804.0
5	2068.0
6	2627.0
7	3318.0
8	3841.0
9	4674.0
10	4829.0
11	5067.0
12	5101.0
13	5342.0
14	5421.0
15	5767.0
16	6139.0
17	6748.0
18	7620.0
19	8763.0
20	10131.0
21	12059.0
22	14396.0
23	17479.0
24	21035.0
25	25492.0
26	30734.0
27	37016.0
28	45005.0
29	56049.0
30	69085.0
31	89305.0
32	118792.0
33	168007.0
34	281801.0
35	699214.0
36	1429252.0
37	658346.0
38	116169.0
39	14500.0
40	70.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.585139790267014	18.682893309439788	16.693545236633252	29.038421663659943
2	32.83855004837877	22.284168853680868	18.848224801867275	26.029056296073083
3	30.297260714210626	19.610882456296675	19.583275349231155	30.508581480261544
4	32.396097411077825	18.241542818349043	18.199127725482512	31.163232045090624
5	32.18981311304025	18.8008226459342	18.740467919285813	30.268896321739735
6	31.32947696237961	17.973293037810585	17.295633528304137	33.40159647150567
7	33.39662699674154	17.743084462248326	17.831116553287075	31.029171987723064
8	30.733099485723887	18.523279638857428	18.6395607757985	32.104060099620185
9	31.5243814044392	17.492858675510583	18.23409423168262	32.748665688367595
10-14	31.71591731915254	18.337015477013168	18.286709804877244	31.660357398957057
15-19	31.815772970791006	18.03993770727925	18.172579415163693	31.971709906766055
20-24	31.75052958471915	18.099448087492917	18.199352934303484	31.95066939348445
25-29	31.88340438832081	18.20983152104096	18.117956281673095	31.788807808965135
30-34	31.986406050757495	17.99197280689763	17.994314555246948	32.027306587097925
35-39	31.857555048002485	18.103276351744384	18.084403594097353	31.95476500615578
40-44	31.99479518052042	18.103107781986367	18.00434205102918	31.89775498646404
45-49	32.05414883333011	17.934646963070737	17.90862300612808	32.102581197471075
50-54	31.903841828441514	18.03014982282154	18.048315560469774	32.017692788267176
55-59	32.020202909403814	18.056989254518665	17.99301674259172	31.9297910934858
60-64	32.07859349431219	17.936746524095827	17.871526445831734	32.11313353576024
65-69	31.91969	18.037615000000002	18.02233	32.020365
70-74	32.054115	18.06768	17.95667	31.921535000000002
75-79	32.09695203085355	17.935731693151908	17.855183676628688	32.11213259936584
80-84	31.950607631675034	18.015390875659442	18.003545355641112	32.030456137024416
85-89	32.07974557544026	18.06186973611342	17.92944444477778	31.928940243668535
90-94	32.129324999999994	17.92784	17.82927	32.113565
95-99	32.01067077530887	17.997309954129808	17.958476404743394	32.033542865817935
100-101	32.25594134276484	18.09088961189786	17.83414169714647	31.819027348190826
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	64.62356145392695
2	58.867606344451865
3	60.80584219447217
4	63.55932945616844
5	62.45870943477999
6	64.73107343388527
7	64.4257989844646
8	62.83715958534407
9	64.2730470928068
10-14	63.37627471810959
15-19	63.78748287755705
20-24	63.7011989782036
25-29	63.67221219728595
30-34	64.01371263785542
35-39	63.81232005415826
40-44	63.89255016698445
45-49	64.15673003080119
50-54	63.92153461670868
55-59	63.94999400288962
60-64	64.19172703007243
65-69	63.940054999999994
70-74	63.975649999999995
75-79	64.20908463021941
80-84	63.98106376869944
85-89	64.0086858191088
90-94	64.24289
95-99	64.0442136411268
100-101	64.07496869095567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	9.0
2	7.5
3	7.0
4	6.5
5	8.5
6	9.0
7	9.0
8	12.0
9	16.0
10	20.0
11	26.5
12	38.0
13	46.5
14	55.5
15	78.5
16	112.5
17	146.0
18	191.0
19	229.0
20	282.0
21	362.5
22	462.0
23	599.0
24	766.0
25	961.5
26	1217.0
27	1495.5
28	1844.0
29	2314.0
30	2799.0
31	3358.5
32	3995.0
33	4719.0
34	5575.0
35	6366.0
36	7165.5
37	8066.0
38	8937.0
39	9906.0
40	10908.0
41	11941.5
42	12965.0
43	14204.0
44	15678.5
45	17334.0
46	19593.0
47	22422.5
48	25768.5
49	29925.5
50	34728.0
51	40410.0
52	47112.5
53	54767.0
54	63449.5
55	72714.0
56	82877.0
57	93849.0
58	105875.5
59	119028.5
60	132790.0
61	146945.5
62	161314.5
63	175459.5
64	188972.5
65	200387.0
66	207946.5
67	212085.5
68	212506.0
69	207914.5
70	198134.5
71	184054.5
72	166328.0
73	145525.0
74	123462.5
75	101080.5
76	79784.0
77	60735.0
78	44140.0
79	30674.5
80	20279.0
81	12723.5
82	7538.0
83	4348.5
84	2499.5
85	1416.5
86	853.5
87	563.0
88	410.0
89	308.5
90	223.0
91	190.0
92	166.0
93	121.5
94	93.5
95	80.0
96	61.5
97	44.5
98	29.5
99	18.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1683
2	0.11115000000000001
3	0.1163
4	0.094525
5	0.090675
6	0.1047
7	0.12165
8	0.155775
9	0.149825
10-14	0.08084999999999999
15-19	0.06537
20-24	0.03488
25-29	0.049240000000000006
30-34	0.07466
35-39	0.041104999999999996
40-44	0.031115
45-49	0.015205000000000002
50-54	0.031585
55-59	0.03519
60-64	1.1999999999999999E-4
65-69	0.0
70-74	0.0
75-79	0.003745
80-84	0.004390000000000001
85-89	2.2E-4
90-94	0.0
95-99	0.00914
100-101	0.008925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2214003698335844
#Duplication Level	Relative count
1	100.0
2	0.17030058052462596
3	0.004007072482932376
4	5.00884060366547E-4
5	0.001001768120733094
6	0.0
7	0.0
8	5.00884060366547E-4
9	0.0
10++	5.00884060366547E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	251300	3.321426	4.030442	1
TTTTT	238260	3.2318451	3.5816755	10-14
GATCG	1278835	3.095444	4.448722	1
CGATC	1265850	3.077747	3.4790466	6
TCGAT	709300	3.0488193	3.4685376	3
ATCGA	711555	3.0426831	3.6181128	2
>>END_MODULE