##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74940375	34.0	31.0	34.0	31.0	34.0
2	33.00162025	34.0	31.0	34.0	31.0	34.0
3	33.0515245	34.0	31.0	34.0	31.0	34.0
4	36.4522135	37.0	37.0	37.0	35.0	37.0
5	36.380739	37.0	37.0	37.0	35.0	37.0
6	36.3568165	37.0	37.0	37.0	35.0	37.0
7	36.357139	37.0	37.0	37.0	35.0	37.0
8	36.342054	37.0	37.0	37.0	35.0	37.0
9	38.137624	39.0	39.0	39.0	37.0	39.0
10-14	38.37610995	39.4	38.6	39.4	36.2	39.4
15-19	39.52426825	41.0	40.0	41.0	37.0	41.0
20-24	39.07867555	40.8	38.8	41.0	35.4	41.0
25-29	37.1642394	40.0	36.2	41.0	28.6	41.0
30-34	33.7283863	37.8	28.0	40.2	20.4	41.0
35-39	36.24275395	37.6	35.0	40.0	30.8	41.0
40-44	26.837960450000004	26.2	23.2	30.6	16.8	38.8
45-49	31.9501849	33.0	30.6	35.6	25.4	38.4
50-54	32.387746	35.0	32.0	35.4	22.0	38.6
55-59	29.787413400000002	34.0	26.6	35.0	16.0	36.0
60-64	32.3946785	35.0	33.0	35.0	26.0	35.4
65-69	32.4198225	35.0	33.0	35.0	26.6	35.0
70-74	31.3257028	35.0	31.8	35.0	21.2	35.0
75-79	31.10524075	34.4	31.8	35.0	22.2	35.0
80-84	31.2623853	35.0	32.0	35.0	23.2	35.0
85-89	30.415872049999997	34.6	31.0	35.0	19.0	35.0
90-94	30.658048	34.6	31.2	35.0	21.6	35.0
95-99	30.41345005	35.0	31.4	35.0	18.6	35.0
100-101	29.046924124999997	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	18.0
4	55.0
5	173.0
6	533.0
7	1745.0
8	4401.0
9	7104.0
10	8579.0
11	10201.0
12	10979.0
13	9314.0
14	8042.0
15	8194.0
16	8576.0
17	8808.0
18	9968.0
19	11040.0
20	12419.0
21	14301.0
22	17765.0
23	22383.0
24	28869.0
25	36752.0
26	46422.0
27	57723.0
28	72488.0
29	93812.0
30	124130.0
31	178479.0
32	298065.0
33	559329.0
34	883481.0
35	815222.0
36	459897.0
37	144455.0
38	24165.0
39	2067.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.758555429742046	18.77535720144521	16.795544934761473	28.67054243405127
2	32.4283	21.919674999999998	19.839875000000003	25.81215
3	29.714374999999997	19.699225000000002	20.307475	30.278925
4	31.99665	18.60825	18.9056	30.489500000000003
5	32.16145	18.82865	18.912925	30.096974999999997
6	31.291725	18.0279	17.53785	33.142525
7	33.3108	17.934175	17.9588	30.796225
8	30.618174999999997	18.538025	18.784200000000002	32.059599999999996
9	31.423875000000002	17.45335	18.4576	32.665175000000005
10-14	31.69419	18.338905	18.362975000000002	31.603930000000002
15-19	31.827855	18.038075	18.200084999999998	31.933985
20-24	32.038331541039334	18.08430896978521	18.180386328484364	31.696973160691094
25-29	32.129657578914724	18.089301574226884	18.045991247233918	31.735049599624475
30-34	32.63835230170292	17.98034151905109	17.843644362830634	31.53766181641535
35-39	31.998273487673924	18.1126215732426	18.09356812647408	31.7955368126094
40-44	39.09866408535445	16.584097342799595	16.1354001081478	28.181838463698156
45-49	32.13298	17.976085	17.985885	31.905050000000003
50-54	32.23627700417727	18.093156895916806	18.03128663296819	31.639279466937737
55-59	32.216459742916705	18.47573003045709	17.94502756410318	31.362782662523024
60-64	32.24810763431186	17.99355129460176	17.876519863388822	31.88182120769756
65-69	32.008473502453356	18.0556168106336	18.009602996088375	31.926306690824667
70-74	32.11562	18.20971	17.93225	31.74242
75-79	32.234365	18.08419	17.852915	31.82853
80-84	32.0450729307115	18.027627280119113	17.994572184259333	31.932727604910056
85-89	32.16545033821645	18.15301456573904	17.978695934022042	31.70283916202247
90-94	32.197431818304565	17.860567705217196	17.86786890407405	32.07413157240419
95-99	32.07933028726331	17.930129723142695	17.967999764799742	32.02254022479425
100-101	32.411525000000005	17.957862499999997	17.859975	31.7706375
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	64.42909786379332
2	58.240449999999996
3	59.993300000000005
4	62.486149999999995
5	62.258424999999995
6	64.43425
7	64.107025
8	62.677775000000004
9	64.08905
10-14	63.298120000000004
15-19	63.76184000000001
20-24	63.73530470173042
25-29	63.86470717853919
30-34	64.17601411811827
35-39	63.793810300283326
40-44	67.28050254905261
45-49	64.03803
50-54	63.875556471115004
55-59	63.57924240543973
60-64	64.12992884200942
65-69	63.934780193278016
70-74	63.85804
75-79	64.062895
80-84	63.977800535621554
85-89	63.868289500238916
90-94	64.27156339070875
95-99	64.10187051205757
100-101	64.18216249999999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	3.5
4	2.5
5	4.0
6	5.5
7	6.5
8	9.0
9	11.5
10	12.5
11	14.0
12	17.5
13	21.5
14	31.5
15	50.5
16	64.0
17	81.5
18	118.5
19	141.0
20	170.5
21	225.5
22	311.0
23	406.0
24	528.5
25	717.5
26	899.0
27	1156.0
28	1526.0
29	1933.5
30	2340.0
31	2877.0
32	3544.5
33	4273.5
34	5046.5
35	5895.5
36	6805.5
37	7716.0
38	8699.5
39	9693.5
40	10741.0
41	11884.0
42	12922.0
43	14009.0
44	15497.5
45	17413.5
46	19561.5
47	22338.5
48	25886.5
49	29902.0
50	34947.5
51	41090.5
52	47804.5
53	55634.0
54	64738.0
55	74225.0
56	84764.5
57	96305.5
58	108644.5
59	122261.0
60	136662.0
61	151370.0
62	166061.5
63	180642.0
64	193677.0
65	204373.5
66	212243.5
67	215509.5
68	213995.5
69	207916.0
70	197035.5
71	181298.5
72	162010.5
73	140825.5
74	118632.0
75	96084.5
76	74680.5
77	56095.0
78	40366.0
79	27678.0
80	18187.0
81	11440.5
82	6794.5
83	3881.5
84	2285.0
85	1429.0
86	926.0
87	603.5
88	428.5
89	305.5
90	202.0
91	145.5
92	105.0
93	72.5
94	45.0
95	25.5
96	14.5
97	8.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0284
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.002455
25-29	7.549999999999999E-4
30-34	3.278895
35-39	0.018090000000000002
40-44	1.8380400000000001
45-49	0.0
50-54	4.25E-4
55-59	1.6501649999999999
60-64	0.01404
65-69	0.008289999999999999
70-74	0.0
75-79	0.0
80-84	2.9E-4
85-89	0.00782
90-94	0.01642
95-99	1.1E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.029495723120146432
#Duplication Level	Relative count
1	100.0
2	0.010001500225033755
3	5.000750112516877E-4
4	5.000750112516877E-4
5	0.0010001500225033755
6	0.0
7	0.0
8	0.0
9	5.000750112516877E-4
10++	0.002500375056258439
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	231970	3.1508255	4.0003285	70-74
TTTTT	223305	3.1224983	3.8716412	95-97
GATCG	1233295	3.0006568	4.438575	1
>>END_MODULE