##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002005_Nocton-Soy_GAGTGG_L002_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	63	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82595075	34.0	31.0	34.0	31.0	34.0
2	33.06775925	34.0	31.0	34.0	31.0	34.0
3	33.10394725	34.0	33.0	34.0	31.0	34.0
4	36.4622705	37.0	37.0	37.0	35.0	37.0
5	36.3931445	37.0	37.0	37.0	35.0	37.0
6	36.35973725	37.0	37.0	37.0	35.0	37.0
7	36.35641175	37.0	37.0	37.0	35.0	37.0
8	36.33966625	37.0	37.0	37.0	35.0	37.0
9	38.138427	39.0	39.0	39.0	37.0	39.0
10-14	38.3999551	39.4	38.8	39.4	37.0	39.4
15-19	39.535457550000004	41.0	40.0	41.0	37.0	41.0
20-24	39.2763956	40.8	39.0	41.0	35.8	41.0
25-29	38.741991	40.0	38.2	41.0	34.8	41.0
30-34	37.8928536	39.8	36.8	41.0	33.2	41.0
35-39	36.76962905	38.2	35.0	40.4	32.2	41.0
40-44	35.829052950000005	36.2	35.0	39.8	31.4	41.0
45-49	35.111027050000004	35.0	35.0	38.0	31.4	40.8
50-54	34.23214535	35.0	34.4	35.8	30.6	39.4
55-59	33.59872614999999	35.0	34.0	35.0	30.0	37.4
60-64	33.13142225	35.0	33.2	35.0	28.6	35.6
65-69	32.71315705	35.0	33.0	35.0	28.4	35.0
70-74	32.41211695	35.0	33.0	35.0	26.8	35.0
75-79	31.67012305	34.4	32.2	35.0	26.0	35.0
80-84	31.977838050000003	35.0	33.0	35.0	26.6	35.0
85-89	31.58255005	35.0	33.0	35.0	24.4	35.0
90-94	31.0797205	35.0	32.6	35.0	23.6	35.0
95-99	30.4223885	35.0	31.4	35.0	18.6	35.0
100-101	29.060439625	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	18.0
4	47.0
5	148.0
6	429.0
7	1198.0
8	2673.0
9	4262.0
10	5292.0
11	5984.0
12	6277.0
13	6669.0
14	7174.0
15	7591.0
16	8519.0
17	9235.0
18	10364.0
19	10819.0
20	11837.0
21	13093.0
22	15238.0
23	17852.0
24	22240.0
25	27767.0
26	34059.0
27	41724.0
28	51162.0
29	63140.0
30	78504.0
31	99838.0
32	131483.0
33	183291.0
34	303789.0
35	735603.0
36	1379018.0
37	590925.0
38	103061.0
39	9631.0
40	17.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.63325710985058	18.76743002671781	16.834545865990865	28.764766997440745
2	32.464025	21.891425	19.84735	25.797199999999997
3	29.7393	19.676099999999998	20.315525	30.269075
4	31.933650000000004	18.62855	18.946425	30.491374999999998
5	32.1199	18.86265	18.926775000000003	30.090675
6	31.28625	18.032500000000002	17.5594	33.12185
7	33.3144	17.950325	17.965600000000002	30.769675000000003
8	30.629725	18.554825	18.797449999999998	32.018
9	31.430225	17.481775	18.462725	32.625274999999995
10-14	31.695769730693407	18.3468953631698	18.36023539251786	31.597099513618932
15-19	31.785225	18.061854999999998	18.21604	31.936880000000002
20-24	31.719780344231772	18.11269580720125	18.279142147094284	31.888381701472696
25-29	31.88069158242054	18.182563638972738	18.18628365199278	31.750461126613942
30-34	31.973887056144417	17.976979315245174	18.059156378523614	31.989977250086792
35-39	31.870694587756077	18.066411651143007	18.151613519763306	31.911280241337618
40-44	31.980400640265216	18.090132434579342	18.073957302752017	31.85550962240342
45-49	32.06565	17.92348	17.961285	32.049585
50-54	31.940230117936032	18.008101273772322	18.073547677597684	31.978120930693965
55-59	32.03141	18.04391	18.035275000000002	31.889405
60-64	32.10425042136556	17.90776644845938	17.91782801403898	32.07015511613607
65-69	31.964834506676688	17.991381412504953	18.029354288079347	32.014429792739016
70-74	32.08711	18.042965	17.961759999999998	31.908165
75-79	32.141355	17.876269999999998	17.88724	32.095135
80-84	32.01311402226036	17.955126970774764	18.011454643522836	32.020304363442044
85-89	32.129962454668565	17.9866791084334	17.943267944048056	31.940090492849986
90-94	32.218366527560256	17.830980207093248	17.840122381102226	32.11053088424428
95-99	32.11594986722554	17.901261359934963	17.942424294852223	32.04036447798728
100-101	32.469423227284686	17.934735801308634	17.81259746160755	31.78324350979913
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	64.39802410729133
2	58.261225
3	60.008375
4	62.425025
5	62.210575
6	64.4081
7	64.084075
8	62.647725
9	64.0555
10-14	63.292869244312335
15-19	63.722105
20-24	63.60816204570446
25-29	63.63115270903448
30-34	63.96386430623121
35-39	63.78197482909369
40-44	63.83591026266864
45-49	64.115235
50-54	63.918351048629994
55-59	63.920815
60-64	64.17440553750163
65-69	63.979264299415696
70-74	63.99527499999999
75-79	64.23649
80-84	64.0334183857024
85-89	64.07005294751855
90-94	64.32889741180453
95-99	64.15631434521282
100-101	64.25266673708381
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	3.0
2	1.0
3	2.5
4	2.5
5	4.5
6	7.0
7	5.5
8	5.0
9	7.0
10	11.0
11	16.0
12	24.0
13	31.0
14	43.0
15	55.5
16	64.5
17	92.0
18	124.5
19	147.0
20	188.5
21	270.5
22	362.0
23	473.0
24	642.0
25	850.5
26	1066.5
27	1376.5
28	1813.0
29	2229.0
30	2645.5
31	3231.0
32	3917.0
33	4695.5
34	5575.0
35	6362.5
36	7229.0
37	8140.0
38	9022.0
39	9913.5
40	10856.5
41	11897.5
42	12969.0
43	14184.0
44	15739.0
45	17638.0
46	19864.0
47	22769.5
48	26215.0
49	30245.5
50	35252.5
51	41039.5
52	47878.5
53	55887.0
54	64273.0
55	73643.0
56	83805.5
57	94689.0
58	106738.5
59	119957.0
60	133725.5
61	147625.0
62	162152.5
63	176424.5
64	188959.0
65	199669.0
66	208002.5
67	211779.5
68	211187.5
69	206200.0
70	196456.5
71	182726.5
72	164928.5
73	143947.0
74	122087.0
75	100082.5
76	79150.0
77	60193.5
78	43850.5
79	30751.0
80	20508.5
81	13030.0
82	7918.0
83	4721.5
84	2766.0
85	1659.5
86	1047.5
87	682.0
88	486.0
89	347.5
90	247.0
91	184.5
92	116.0
93	84.5
94	55.0
95	25.0
96	15.5
97	10.0
98	4.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019875
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.2E-4
15-19	0.0
20-24	8.049999999999999E-4
25-29	3.5E-4
30-34	0.063355
35-39	0.01393
40-44	8.15E-4
45-49	0.0
50-54	0.0021449999999999998
55-59	0.0
60-64	0.01556
65-69	0.02074
70-74	0.0
75-79	0.0
80-84	0.004745
85-89	0.0142
90-94	0.02378
95-99	0.00713
100-101	6.875000000000001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.17546753850537353
#Duplication Level	Relative count
1	100.0
2	0.15023587031639674
3	0.003004717406327935
4	0.0015023587031639675
5	0.0010015724687759784
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0015023587031639675
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	239385	3.1952634	4.0653467	1
TTTTT	237500	3.1523325	3.4430783	90-94
GATCG	1280555	3.095388	4.4829407	1
CGATC	1262975	3.0712602	3.4449396	1
ATCGA	713015	3.0513034	3.8056803	2
TCGAT	705675	3.0164993	3.347474	5
>>END_MODULE