##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_R2_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	71944599	
Filtered Sequences	0	
Sequence length	30-101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73050114574966	34.0	31.0	34.0	31.0	34.0
2	32.85029655944013	34.0	31.0	34.0	31.0	34.0
3	32.88082150544754	34.0	31.0	34.0	31.0	34.0
4	36.257947549335846	37.0	37.0	37.0	35.0	37.0
5	36.25183302501971	37.0	37.0	37.0	35.0	37.0
6	36.2452585634677	37.0	37.0	37.0	35.0	37.0
7	36.24601246300643	37.0	37.0	37.0	35.0	37.0
8	36.23168318722577	37.0	37.0	37.0	35.0	37.0
9	37.99503460989476	39.0	39.0	39.0	37.0	39.0
10-14	38.272135530284906	39.4	38.6	39.4	35.4	39.4
15-19	39.42842647020662	41.0	39.8	41.0	36.8	41.0
20-24	39.19079256248269	41.0	39.0	41.0	35.6	41.0
25-29	38.88897485410962	40.2	38.4	41.0	35.0	41.0
30-34	38.289779411130766	40.0	37.4	41.0	34.0	41.0
35-39	37.34505057720354	38.8	35.2	40.8	33.0	41.0
40-44	36.46434542696095	37.0	35.0	40.0	32.0	41.0
45-49	35.58728274604245	35.0	35.0	39.0	31.4	41.0
50-54	34.37371994379135	34.8	33.8	36.2	29.6	39.8
55-59	34.60345323077145	35.0	35.0	35.2	31.0	39.2
60-64	34.3020305770897	35.0	35.0	35.0	31.0	37.2
65-69	34.04476817265869	35.0	34.2	35.0	31.0	36.0
70-74	33.83767004269148	35.0	34.0	35.0	31.0	35.0
75-79	33.639481649418286	35.0	34.0	35.0	30.0	35.0
80-84	33.48182473908722	35.0	33.2	35.0	30.0	35.0
85-89	33.35594255978509	35.0	33.0	35.0	29.4	35.0
90-94	33.246244626356024	35.0	33.0	35.0	29.0	35.0
95-99	33.27667290548105	35.0	33.0	35.0	29.2	35.0
100-101	32.9790901817986	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	9.0
16	56.0
17	156.0
18	937.0
19	5004.0
20	15755.0
21	39676.0
22	82180.0
23	149605.0
24	240658.0
25	359322.0
26	496759.0
27	657254.0
28	840616.0
29	1057875.0
30	1321831.0
31	1677504.0
32	2205280.0
33	3107597.0
34	5097687.0
35	1.2083128E7
36	2.4939026E7
37	1.3199376E7
38	3639764.0
39	723117.0
40	4425.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.625428880352786	22.98268977772744	19.038706435767335	27.35317490615244
2	30.691603003519003	23.594257202545478	22.09648310747148	23.61765668646404
3	29.322691282983527	21.749506902174033	21.045738979048288	27.88206283579415
4	31.19311153370382	20.517791120911465	19.42892454925772	28.860172796127
5	30.737689886888624	19.83395924894131	19.441756926263825	29.986593937906243
6	30.162826863511256	19.079317909161375	18.346898600421046	32.41095662690633
7	32.01860406677837	18.939316852714473	19.16673477640322	29.875344304103933
8	29.619443805158678	19.317233529661664	19.830955537925945	31.232367127253713
9	30.387809166863676	18.293555456371855	19.571172109303504	31.74746326746096
10-14	30.555980630598366	19.28959620733445	19.418254117480878	30.736169044586305
15-19	30.652569552464502	19.165184716500537	19.321622674325724	30.860623056709237
20-24	30.621769063449644	19.187714130761112	19.33640995732951	30.854106848459733
25-29	30.699807710456277	19.259253314177723	19.276170726328967	30.76476824903703
30-34	30.76889891272736	19.141734009984162	19.172706413295145	30.91666066399333
35-39	30.70171363026889	19.209268249874086	19.245434016867872	30.84358410298915
40-44	30.751835095445507	19.244926463452316	19.21368823519829	30.789550205903886
45-49	30.797126314917183	19.145281877670847	19.159709842892926	30.89788196451904
50-54	30.67782344621799	19.218398823706792	19.252745056192804	30.85103267388242
55-59	30.725374854494103	19.275715011050355	19.24137905121501	30.75753108324053
60-64	30.757104466305357	19.191989105073937	19.185682594564273	30.865223834056433
65-69	30.639136134251792	19.266006777605778	19.298600910250553	30.796256177891877
70-74	30.683812253765446	19.333720692759588	19.276245639075597	30.70622141439937
75-79	30.710944323307338	19.251463072295767	19.239611686996767	30.79798091740013
80-84	30.582384947370528	19.334206604473877	19.345289582652118	30.738118865503477
85-89	30.614452026813126	19.403031609673107	19.345257418720625	30.637258944793146
90-94	30.61783130641243	19.338184582069136	19.317943440136748	30.72604067138169
95-99	30.420604323759132	19.42972757436351	19.469733898617882	30.679934203259474
100-101	30.746978871772146	19.38696894718547	19.268975150714148	30.59707703032824
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.978603786505225
2	54.30925968998304
3	57.20475411877768
4	60.05328432983082
5	60.72428382479487
6	62.573783490417576
7	61.89394837088231
8	60.851810932412384
9	62.13527243432464
10-14	61.29214967518467
15-19	61.51319260917374
20-24	61.47587591190937
25-29	61.4645759594933
30-34	61.68555957672069
35-39	61.54529773325804
40-44	61.54138530134939
45-49	61.69500827943622
50-54	61.52885612010041
55-59	61.482905937734635
60-64	61.62232830036179
65-69	61.43539231214367
70-74	61.39003366816481
75-79	61.50892524070747
80-84	61.32050381287401
85-89	61.251710971606265
90-94	61.343871977794116
95-99	61.1005385270186
100-101	61.34405590210038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	797.0
1	595.5
2	352.0
3	300.5
4	319.0
5	394.0
6	490.5
7	591.5
8	745.0
9	947.0
10	1177.5
11	1498.5
12	1908.5
13	2491.0
14	3271.5
15	4170.0
16	5357.0
17	6895.0
18	8963.5
19	11711.0
20	15179.5
21	19419.5
22	24921.0
23	32186.0
24	40866.0
25	51238.0
26	64335.5
27	79804.5
28	97321.5
29	117982.0
30	141108.5
31	165906.0
32	192090.0
33	219898.0
34	246880.5
35	272597.5
36	298286.0
37	322777.5
38	346108.0
39	367231.5
40	387411.5
41	406315.5
42	423708.0
43	443885.0
44	469339.0
45	500983.0
46	541271.0
47	592153.0
48	656219.5
49	736636.5
50	833596.5
51	948836.5
52	1084067.5
53	1235806.5
54	1399861.0
55	1576766.5
56	1769703.5
57	1973501.0
58	2186100.0
59	2408142.5
60	2635869.0
61	2866559.0
62	3090469.0
63	3297670.5
64	3477301.0
65	3611450.0
66	3687077.5
67	3686062.0
68	3603661.5
69	3441459.0
70	3198110.5
71	2886097.0
72	2522311.5
73	2127949.0
74	1726376.5
75	1343245.5
76	996512.5
77	699043.0
78	461341.0
79	284940.0
80	164415.5
81	89525.5
82	46759.5
83	24575.5
84	14016.5
85	8652.5
86	5383.5
87	3595.0
88	2496.5
89	1573.5
90	994.0
91	697.5
92	474.0
93	289.0
94	217.0
95	153.5
96	87.5
97	67.5
98	67.0
99	70.0
100	74.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009889554044216717
3	0.014024680296014993
4	0.022426978847988298
5	0.024236704689951778
6	0.0315284264771564
7	0.03661845415247919
8	0.045519747771476216
9	0.039837597816063997
10-14	0.015143874802888263
15-19	0.0059187208757672
20-24	0.002445492815937441
25-29	0.002993414418780762
30-34	0.011086257961079232
35-39	0.0010293390677674364
40-44	3.3486075453685073E-4
45-49	3.442091493819827E-5
50-54	3.182091621232309E-4
55-59	6.638559760977701E-4
60-64	2.8141501525722465E-7
65-69	1.1511067379211894E-4
70-74	0.0
75-79	4.5459857154729876E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	51434.0
32-33	53922.0
34-35	55852.0
36-37	57277.0
38-39	56701.0
40-41	57658.0
42-43	57087.0
44-45	57475.0
46-47	57957.0
48-49	58118.0
50-51	44990.0
52-53	40252.0
54-55	47979.0
56-57	53859.0
58-59	59599.0
60-61	63579.0
62-63	68886.0
64-65	73154.0
66-67	79421.0
68-69	85771.0
70-71	93640.0
72-73	103534.0
74-75	111563.0
76-77	123239.0
78-79	135098.0
80-81	149705.0
82-83	166632.0
84-85	189670.0
86-87	224330.0
88-89	266374.0
90-91	330591.0
92-93	421391.0
94-95	560521.0
96-97	762122.0
98-99	1148122.0
100-101	6.5977096E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	4.029827621537057
#Duplication Level	Relative count
1	100.0
2	3.9646911780022913
3	0.11040516612852828
4	0.0161441516508697
5	0.013019477137798146
6	0.010936360795750442
7	0.011457139881262368
8	0.005728569940631184
9	0.003645453598583481
10++	0.019789605249453182
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	6250610	3.372844	3.7491143	10-14
AAAAA	6263830	3.3415248	3.8971362	1
>>END_MODULE