##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.434848	34.0	31.0	34.0	31.0	34.0
2	32.5963115	34.0	31.0	34.0	31.0	34.0
3	32.63935425	34.0	31.0	34.0	31.0	34.0
4	36.0454775	37.0	37.0	37.0	35.0	37.0
5	36.04087375	37.0	37.0	37.0	35.0	37.0
6	36.0459465	37.0	37.0	37.0	35.0	37.0
7	36.03033925	37.0	37.0	37.0	35.0	37.0
8	35.989181	37.0	37.0	37.0	35.0	37.0
9	37.72274625	39.0	38.0	39.0	35.0	39.0
10-14	37.9888377	39.4	38.2	39.4	35.2	39.4
15-19	39.0849841	41.0	39.0	41.0	36.0	41.0
20-24	38.83971525	40.8	39.0	41.0	35.0	41.0
25-29	38.4572052	40.0	38.0	41.0	34.4	41.0
30-34	37.81238165	40.0	37.0	41.0	33.2	41.0
35-39	36.833002500000006	38.6	35.0	40.6	32.0	41.0
40-44	35.94219645	36.6	35.0	40.0	31.0	41.0
45-49	35.0165344	35.0	34.8	38.8	30.4	41.0
50-54	33.7519005	35.0	33.2	36.2	28.4	39.8
55-59	33.859555099999994	35.0	34.0	35.0	30.0	39.0
60-64	33.56693865	35.0	34.0	35.0	30.0	37.2
65-69	33.250268250000005	35.0	34.0	35.0	29.2	35.8
70-74	32.9550145	35.0	33.6	35.0	29.0	35.0
75-79	32.6802702	35.0	33.0	35.0	28.0	35.0
80-84	32.37713695	35.0	33.0	35.0	27.0	35.0
85-89	32.092672900000004	35.0	33.0	35.0	27.0	35.0
90-94	31.7226129	35.0	33.0	35.0	25.2	35.0
95-99	31.193216200000002	35.0	33.0	35.0	23.8	35.0
100-101	30.120828125000003	34.0	31.5	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9639.0
3	1739.0
4	1935.0
5	2407.0
6	2977.0
7	3643.0
8	4508.0
9	4966.0
10	5252.0
11	5380.0
12	5371.0
13	5434.0
14	5432.0
15	5439.0
16	5918.0
17	6460.0
18	7289.0
19	8479.0
20	9715.0
21	11734.0
22	14194.0
23	17466.0
24	21412.0
25	25772.0
26	31713.0
27	38851.0
28	47525.0
29	58277.0
30	72176.0
31	90969.0
32	120234.0
33	166182.0
34	268753.0
35	637503.0
36	1342437.0
37	705777.0
38	190613.0
39	36225.0
40	204.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.622450628559367	22.986606309758418	19.007948360422397	27.382994701259822
2	30.74111209843336	23.584234931994843	22.056470496409617	23.61818247316218
3	29.292014588972222	21.710551989473526	21.080339408736375	27.917094012817884
4	31.27760940453237	20.469156011458498	19.407278927151694	28.845955656857434
5	30.761394581983563	19.825592214127504	19.42921543008182	29.98379777380712
6	30.20345685088267	19.062720066900336	18.27865671164034	32.455166370576656
7	32.032568046295026	18.912725452180382	19.158697168060158	29.89600933346444
8	29.672107436775697	19.341592413684037	19.76659106241655	31.21970908712372
9	30.45037622287451	18.287655324584833	19.521642325974394	31.74032612656626
10-14	30.591277142960482	19.28416428494495	19.36724835689091	30.757310215203653
15-19	30.67308481196809	19.146027146713518	19.28576288348285	30.895125157835544
20-24	30.670887845570675	19.160429223769416	19.306116416206105	30.862566514453803
25-29	30.736721016805234	19.271167002728646	19.231001627422977	30.761110353043147
30-34	30.815074055845376	19.123376581145255	19.1163776736552	30.94517168935417
35-39	30.752447358526435	19.183705461346083	19.202467713894805	30.861379466232673
40-44	30.796176069486435	19.218617685168496	19.167724768546258	30.817481476798815
45-49	30.868044667759904	19.107915079444858	19.0915615061891	30.932478746606133
50-54	30.73992230855272	19.17160599068799	19.207131712350787	30.881339988408502
55-59	30.783858092132853	19.219887601194984	19.191387325794004	30.80486698087816
60-64	30.82699563878081	19.150204260898747	19.10898907955195	30.913811020768488
65-69	30.72898	19.21602	19.21079	30.84421
70-74	30.78879577607852	19.264981742743608	19.175451711408098	30.77077076976977
75-79	30.82900643742906	19.164885797799336	19.139284687991225	30.86682307678038
80-84	30.74050834452791	19.241679674779835	19.20254532303992	30.81526665765233
85-89	30.812202058436483	19.277605724458034	19.183160426955347	30.72703179015014
90-94	30.851646015092015	19.18288174904538	19.132236361608165	30.833235874254438
95-99	30.764077627569126	19.230201873603377	19.230026858342047	30.775693640485454
100-101	30.885662886143916	19.31885586506475	19.17649716338911	30.618984085402218
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.00544532981918
2	54.35929457159554
3	57.209108601790106
4	60.123565061389804
5	60.74519235579068
6	62.65862322145932
7	61.92857737975947
8	60.89181652389942
9	62.19070234944078
10-14	61.34858735816414
15-19	61.56820996980363
20-24	61.53345436002448
25-29	61.49783136984838
30-34	61.76024574519955
35-39	61.61382682475911
40-44	61.61365754628525
45-49	61.80052341436604
50-54	61.62126229696122
55-59	61.58872507301102
60-64	61.7408066595493
65-69	61.57319
70-74	61.559566545848284
75-79	61.69582951420944
80-84	61.55577500218025
85-89	61.539233848586626
90-94	61.68488188934646
95-99	61.53977126805458
100-101	61.50464697154614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	101.5
2	45.0
3	45.0
4	42.5
5	47.5
6	57.0
7	54.5
8	71.0
9	95.5
10	110.5
11	137.0
12	155.5
13	183.5
14	244.0
15	326.5
16	410.5
17	489.5
18	620.5
19	751.5
20	924.0
21	1169.5
22	1459.0
23	1876.5
24	2367.5
25	2876.0
26	3611.5
27	4489.0
28	5365.0
29	6462.5
30	7766.0
31	9270.0
32	10705.5
33	12133.0
34	13654.0
35	15009.5
36	16316.5
37	17656.0
38	18964.0
39	20236.0
40	21331.0
41	22239.0
42	23192.5
43	24310.0
44	25616.0
45	27207.0
46	29379.0
47	32129.0
48	35681.0
49	39892.5
50	45020.5
51	51184.0
52	58409.0
53	66668.5
54	75473.5
55	85019.0
56	95157.0
57	105868.0
58	117193.0
59	129178.0
60	141797.0
61	154551.5
62	166594.5
63	177586.0
64	187188.0
65	194469.5
66	199074.5
67	199457.5
68	195247.5
69	186931.0
70	174156.0
71	157405.5
72	137667.5
73	116541.0
74	94988.0
75	74124.5
76	55427.5
77	38979.5
78	25461.0
79	15760.0
80	9004.5
81	4729.5
82	2395.0
83	1201.5
84	672.5
85	447.0
86	344.0
87	249.0
88	187.0
89	158.5
90	135.5
91	112.0
92	91.0
93	84.0
94	65.0
95	48.0
96	47.5
97	37.5
98	24.0
99	16.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061149999999999996
2	0.0664
3	0.0615
4	0.0567
5	0.0446
6	0.030400000000000003
7	0.079975
8	0.09380000000000001
9	0.1124
10-14	0.053045
15-19	0.0542
20-24	0.035825
25-29	0.038279999999999995
30-34	0.05583
35-39	0.038655
40-44	0.02538
45-49	0.02185
50-54	0.030180000000000002
55-59	0.01851
60-64	4.4E-4
65-69	0.0
70-74	3.5E-5
75-79	0.004335
80-84	0.011120000000000001
85-89	3.15E-4
90-94	7.65E-4
95-99	0.00872
100-101	0.0061125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.4001178874175181
#Duplication Level	Relative count
1	100.0
2	0.2848688499924771
3	0.009529063644114549
4	0.0025076483273985656
5	0.0020061186619188525
6	0.0010030593309594263
7	0.0010030593309594263
8	0.0010030593309594263
9	5.015296654797131E-4
10++	0.003510707658357992
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	400375	3.8455863	4.3568106	1
TTTTT	372640	3.647414	4.0732026	10-14
>>END_MODULE