##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.451856	34.0	31.0	34.0	31.0	34.0
2	32.63840275	34.0	31.0	34.0	31.0	34.0
3	32.65644125	34.0	31.0	34.0	31.0	34.0
4	36.0639265	37.0	37.0	37.0	35.0	37.0
5	36.0698175	37.0	37.0	37.0	35.0	37.0
6	35.986414	37.0	37.0	37.0	35.0	37.0
7	36.011844	37.0	37.0	37.0	35.0	37.0
8	36.00461925	37.0	37.0	37.0	35.0	37.0
9	37.73818675	39.0	38.0	39.0	35.0	39.0
10-14	38.0047191	39.4	38.2	39.4	35.2	39.4
15-19	39.10483599999999	41.0	39.0	41.0	36.0	41.0
20-24	38.8297997	40.8	39.0	41.0	35.2	41.0
25-29	38.4808818	40.0	38.0	41.0	34.6	41.0
30-34	37.783941649999996	40.0	37.0	41.0	33.2	41.0
35-39	36.7898828	38.6	35.0	40.6	31.8	41.0
40-44	35.887168	36.6	35.0	40.0	31.0	41.0
45-49	34.94497115	35.0	34.8	38.8	30.4	41.0
50-54	33.637662899999995	34.8	33.2	36.2	28.0	39.8
55-59	33.77785225	35.0	34.0	35.0	30.0	39.0
60-64	33.454587249999996	35.0	34.0	35.0	29.4	37.2
65-69	33.12495439999999	35.0	34.0	35.0	29.0	35.8
70-74	32.80395615	35.0	33.0	35.0	28.8	35.0
75-79	32.49682655	35.0	33.0	35.0	27.0	35.0
80-84	32.1597878	35.0	33.0	35.0	27.0	35.0
85-89	31.81822335	35.0	33.0	35.0	25.4	35.0
90-94	31.378102849999998	35.0	33.0	35.0	24.0	35.0
95-99	30.768334949999996	35.0	32.0	35.0	21.4	35.0
100-101	29.6017855	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10985.0
3	2013.0
4	2378.0
5	2705.0
6	3443.0
7	4119.0
8	4835.0
9	5406.0
10	5689.0
11	5901.0
12	5964.0
13	5930.0
14	5910.0
15	6005.0
16	6402.0
17	7129.0
18	7871.0
19	9045.0
20	10291.0
21	12526.0
22	15140.0
23	18382.0
24	22177.0
25	27153.0
26	32426.0
27	39188.0
28	48115.0
29	58906.0
30	73783.0
31	93454.0
32	123385.0
33	174162.0
34	282883.0
35	657157.0
36	1304010.0
37	680378.0
38	188536.0
39	35988.0
40	230.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.630595466053954	22.978989158673098	19.011167099654187	27.37924827561876
2	30.736129079075447	23.61075592233836	22.045887331062904	23.60722766752329
3	29.3146288914871	21.7603769444769	21.009595500765744	27.915398663270256
4	31.201884877809515	20.505253358658564	19.44714922241283	28.845712541119088
5	30.791728264431388	19.815744513734113	19.428622886602582	29.963904335231923
6	30.21531429120489	19.089153663625876	18.259664796417407	32.435867248751826
7	32.084152132716845	18.931225858456393	19.139077821196746	29.84554418763002
8	29.620377281578534	19.32083907455116	19.837567458005335	31.221216185864975
9	30.44837595155714	18.283018929347815	19.51843114118242	31.75017397791263
10-14	30.581921610220597	19.282334980803757	19.388257376909877	30.74748603206577
15-19	30.668618216642766	19.142566420666146	19.3036203377223	30.885195024968787
20-24	30.67585867647647	19.162976959587308	19.297116742637183	30.86404762129904
25-29	30.73535659038798	19.250241938215414	19.22429577505307	30.790105696343538
30-34	30.79678222054688	19.129391462251537	19.146172104327047	30.927654212874533
35-39	30.71933101912585	19.190510932291797	19.22520153439009	30.86495651419226
40-44	30.804074806465103	19.193381528409088	19.178909899679546	30.823633765446264
45-49	30.868697565366414	19.08993293048543	19.102692403956826	30.938677100191324
50-54	30.71976290271492	19.153381485673854	19.213335131525845	30.91352048008538
55-59	30.78899860368794	19.22637618626872	19.192425088355645	30.792200121687696
60-64	30.8462279390691	19.150397033255672	19.103156645884496	30.90021838179073
65-69	30.732060977289642	19.21419126740852	19.208091238433383	30.845656516868452
70-74	30.794835066571174	19.262845083397217	19.18029986877966	30.762019981251953
75-79	30.831106582325653	19.14712677864643	19.13211064793179	30.88965599109613
80-84	30.728151669158503	19.221733167486367	19.22025786829569	30.829857295059437
85-89	30.805101440811526	19.277419219353757	19.191383531068247	30.72609580876647
90-94	30.866362200973157	19.160673304590794	19.123152602953674	30.84981189148237
95-99	30.762467822047334	19.226239825755705	19.212982064163967	30.798310288032997
100-101	30.914905217219214	19.282981631034698	19.181678467032963	30.62043468471312
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.00984374167272
2	54.34335674659874
3	57.230027554757356
4	60.04759741892861
5	60.755632599663315
6	62.65118153995671
7	61.929696320346864
8	60.84159346744351
9	62.19854992946977
10-14	61.32940764228637
15-19	61.55381324161156
20-24	61.5399062977755
25-29	61.525462286731525
30-34	61.72443643342142
35-39	61.584287533318104
40-44	61.62770857191136
45-49	61.807374665557745
50-54	61.6332833828003
55-59	61.58119872537564
60-64	61.746446320859825
65-69	61.5777174941581
70-74	61.55685504782312
75-79	61.72076257342178
80-84	61.55800896421795
85-89	61.53119724957799
90-94	61.71617409245553
95-99	61.56077811008033
100-101	61.53533990193234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	73.0
2	37.0
3	35.0
4	42.0
5	48.0
6	52.5
7	60.5
8	75.5
9	92.0
10	117.0
11	152.0
12	172.0
13	222.5
14	304.0
15	353.0
16	416.5
17	524.0
18	655.0
19	801.5
20	978.0
21	1212.0
22	1500.5
23	1881.5
24	2384.0
25	2996.5
26	3717.0
27	4471.5
28	5432.0
29	6572.0
30	7799.5
31	9225.0
32	10761.5
33	12204.0
34	13686.5
35	15133.5
36	16552.5
37	17973.0
38	18979.5
39	20066.5
40	21315.0
41	22275.0
42	23344.5
43	24551.0
44	25713.0
45	27355.5
46	29474.5
47	31969.5
48	35568.0
49	39886.5
50	44906.5
51	51228.5
52	58453.5
53	66816.5
54	75681.5
55	85092.0
56	95352.0
57	106189.5
58	117683.0
59	129387.5
60	141771.5
61	154303.5
62	166230.0
63	177633.0
64	187418.5
65	194412.5
66	198348.0
67	198551.0
68	194446.0
69	186067.5
70	173272.5
71	156647.0
72	137503.5
73	116325.5
74	94445.0
75	73914.0
76	54878.5
77	38606.5
78	25788.0
79	15955.0
80	9321.0
81	5210.5
82	2802.0
83	1522.0
84	949.0
85	687.5
86	539.5
87	412.0
88	333.0
89	283.0
90	252.0
91	224.0
92	186.0
93	170.0
94	135.0
95	102.5
96	96.0
97	78.5
98	54.5
99	35.5
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.083725
2	0.09225
3	0.080775
4	0.09962499999999999
5	0.057249999999999995
6	0.089075
7	0.13324999999999998
8	0.155475
9	0.166925
10-14	0.08723
15-19	0.086255
20-24	0.063205
25-29	0.062295
30-34	0.09321499999999999
35-39	0.044975
40-44	0.045805
45-49	0.03506
50-54	0.04778
55-59	0.047415
60-64	8.2E-4
65-69	4.7500000000000005E-4
70-74	2.6000000000000003E-4
75-79	0.00753
80-84	0.02028
85-89	7.999999999999999E-4
90-94	0.0018700000000000001
95-99	0.020829999999999998
100-101	0.0154625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3382953313245438
#Duplication Level	Relative count
1	100.0
2	0.258217845610798
3	0.007019514249613927
4	0.0020055754998896933
5	0.005515332624696657
6	0.0010027877499448467
7	5.013938749724233E-4
8	5.013938749724233E-4
9	0.0
10++	0.004011150999779387
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	404715	3.8843303	4.346854	60-64
TTTTT	379795	3.7044911	4.0607715	90-94
>>END_MODULE