##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54310625	34.0	31.0	34.0	31.0	34.0
2	32.736057	34.0	31.0	34.0	31.0	34.0
3	32.7600925	34.0	31.0	34.0	31.0	34.0
4	36.139126	37.0	37.0	37.0	35.0	37.0
5	36.03003025	37.0	37.0	37.0	35.0	37.0
6	36.0853655	37.0	37.0	37.0	35.0	37.0
7	36.0982045	37.0	37.0	37.0	35.0	37.0
8	36.0829915	37.0	37.0	37.0	35.0	37.0
9	37.83521325	39.0	39.0	39.0	35.0	39.0
10-14	37.9890843	39.4	38.6	39.4	35.2	39.4
15-19	39.197021050000004	41.0	39.6	41.0	36.2	41.0
20-24	38.41721485	40.6	38.6	41.0	33.8	41.0
25-29	38.44482395	40.0	38.0	41.0	34.8	41.0
30-34	37.938321499999994	40.0	37.2	41.0	33.4	41.0
35-39	36.70617795	38.6	35.2	40.6	31.8	41.0
40-44	35.83534075000001	36.6	35.0	40.0	30.8	41.0
45-49	34.65279475	35.0	34.0	38.8	29.2	41.0
50-54	33.708182900000004	34.8	33.2	36.2	28.6	39.8
55-59	33.89101945	35.0	34.2	35.2	30.0	39.2
60-64	33.56742195	35.0	34.0	35.0	30.0	37.2
65-69	33.23562735	35.0	34.0	35.0	29.4	36.0
70-74	32.898602200000006	35.0	33.6	35.0	29.0	35.0
75-79	32.4157591	35.0	33.0	35.0	27.0	35.0
80-84	32.20882195	35.0	33.0	35.0	27.0	35.0
85-89	31.8694703	35.0	33.0	35.0	26.2	35.0
90-94	31.421352000000002	35.0	33.0	35.0	24.0	35.0
95-99	30.796044550000005	35.0	32.2	35.0	22.0	35.0
100-101	29.555522375000002	34.0	30.5	35.0	9.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12190.0
3	2014.0
4	2328.0
5	2741.0
6	3284.0
7	3914.0
8	4473.0
9	4938.0
10	5335.0
11	5643.0
12	5697.0
13	5661.0
14	5780.0
15	5749.0
16	6216.0
17	6914.0
18	7577.0
19	8642.0
20	10220.0
21	12012.0
22	14288.0
23	17283.0
24	20753.0
25	25157.0
26	30556.0
27	37141.0
28	45419.0
29	56947.0
30	70928.0
31	91844.0
32	123314.0
33	180530.0
34	310295.0
35	720719.0
36	1280837.0
37	642094.0
38	178222.0
39	32173.0
40	172.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.615693315027986	22.995642052714796	19.043133517754868	27.345531114502347
2	30.71468061127981	23.600610368811626	22.055892744474757	23.628816275433806
3	29.310746491324867	21.74206248816095	21.02737801306155	27.919813007452625
4	31.213006197385063	20.52647231704496	19.377342544462657	28.883178941107317
5	30.952738931485623	19.855392643096963	19.33649329411026	29.855375131307156
6	30.12667767485938	19.103003383829975	18.298520654132016	32.47179828717863
7	32.061584295238376	18.906765311864596	19.144178945768264	29.88747144712876
8	29.64014261926753	19.313572753938175	19.784152206713266	31.26213242008103
9	30.373487371885062	18.285314029412824	19.544493011547882	31.796705587154232
10-14	30.67463921167033	19.298325002680553	19.339431811003188	30.687603974645928
15-19	30.713667491633327	19.148016318332115	19.274111428914235	30.864204761120316
20-24	30.88199918019296	19.13905647926054	19.238732444797016	30.740211895749493
25-29	30.770529120148037	19.238768350369522	19.232318167647705	30.758384361834736
30-34	30.8065670996785	19.121660881416062	19.1367101761168	30.93506184278864
35-39	30.876961037764495	19.20771250779098	19.149606203114374	30.765720251330148
40-44	30.849598282504616	19.25584405500532	19.1262124167808	30.768345245709263
45-49	30.861784063156133	19.113493207814734	19.174863599814422	30.849859129214714
50-54	30.744965978590727	19.16190770836928	19.193966233387965	30.899160079652027
55-59	30.788996396631518	19.208221620371	19.18557047463432	30.81721150836316
60-64	30.83843967075174	19.14111031288825	19.10570028456023	30.914749731799784
65-69	30.71762	19.2024	19.208434999999998	30.871545
70-74	30.80108432314454	19.2506958270179	19.171875598439236	30.77634425139833
75-79	30.894156621225736	19.169972895647568	19.10147074125481	30.83439974187188
80-84	30.735336606110504	19.208717038774655	19.227541700702176	30.828404654412665
85-89	30.825185412592703	19.251214625607314	19.183644591822297	30.739955369977682
90-94	30.883115	19.154905	19.104245	30.857734999999998
95-99	30.797597045425977	19.19090713170642	19.22117588088837	30.790319941979227
100-101	31.041254198857697	19.30689405301225	19.121449192884157	30.53040255524589
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.96122442953033
2	54.343496886713616
3	57.2305594987775
4	60.09618513849237
5	60.808114062792775
6	62.598475962038016
7	61.94905574236714
8	60.90227503934857
9	62.1701929590393
10-14	61.362243186316256
15-19	61.57787225275365
20-24	61.622211075942445
25-29	61.52891348198277
30-34	61.741628942467145
35-39	61.64268128909465
40-44	61.61794352821388
45-49	61.71164319237084
50-54	61.644126058242755
55-59	61.60620790499468
60-64	61.75318940255152
65-69	61.589165
70-74	61.57742857454287
75-79	61.72855636309762
80-84	61.563741260523166
85-89	61.565140782570396
90-94	61.74085
95-99	61.58791698740521
100-101	61.571656754103586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	90.5
2	49.0
3	34.5
4	36.5
5	42.5
6	43.0
7	53.0
8	67.5
9	77.5
10	93.5
11	124.0
12	169.0
13	212.5
14	257.5
15	314.0
16	416.0
17	547.5
18	654.0
19	812.0
20	1020.5
21	1251.0
22	1488.0
23	1885.5
24	2445.0
25	2994.0
26	3668.5
27	4484.0
28	5418.5
29	6607.5
30	7922.0
31	9279.5
32	10734.0
33	12272.0
34	13781.0
35	15151.5
36	16427.0
37	17856.0
38	19222.0
39	20370.0
40	21471.0
41	22459.5
42	23258.5
43	24440.5
44	25732.5
45	27259.5
46	29528.0
47	32161.0
48	35514.5
49	39864.0
50	45020.0
51	51068.5
52	58258.5
53	66392.0
54	75445.0
55	84593.5
56	95003.0
57	106246.5
58	117423.5
59	129561.0
60	141759.0
61	153879.0
62	165732.5
63	177145.0
64	187143.5
65	194717.0
66	198941.5
67	198817.5
68	194685.5
69	185896.5
70	173116.0
71	156749.5
72	137357.0
73	116473.0
74	94685.0
75	73652.0
76	54786.5
77	38759.5
78	25852.5
79	16127.0
80	9437.5
81	5292.0
82	2930.0
83	1677.0
84	1098.0
85	791.5
86	600.5
87	482.0
88	408.5
89	351.5
90	297.5
91	244.0
92	199.5
93	173.5
94	142.5
95	120.5
96	93.0
97	85.0
98	75.5
99	47.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10204999999999999
2	0.10957499999999999
3	0.092275
4	0.1353
5	0.067325
6	0.150125
7	0.17422500000000002
8	0.202825
9	0.20679999999999998
10-14	0.11387000000000001
15-19	0.11111499999999999
20-24	0.086245
25-29	0.08034999999999999
30-34	0.12821
35-39	0.036664999999999996
40-44	0.05911999999999999
45-49	0.041375
50-54	0.057784999999999996
55-59	0.07128
60-64	7.999999999999999E-5
65-69	0.0
70-74	2.9E-4
75-79	0.003145
80-84	0.024765
85-89	4.9999999999999996E-5
90-94	0.0
95-99	0.028905
100-101	0.01745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3641849799923133
#Duplication Level	Relative count
1	100.0
2	0.2622289966105774
3	0.008022301999558773
4	0.0010027877499448467
5	0.0
6	5.013938749724233E-4
7	0.0010027877499448467
8	0.0
9	0.0
10++	0.0060167264996690804
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	401010	3.8514283	4.4811797	60-64
TTTTT	381585	3.7477205	4.1606317	10-14
>>END_MODULE