##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.451346	34.0	31.0	34.0	31.0	34.0
2	32.6184715	34.0	31.0	34.0	31.0	34.0
3	32.65108475	34.0	31.0	34.0	31.0	34.0
4	36.04654975	37.0	37.0	37.0	35.0	37.0
5	36.045196	37.0	37.0	37.0	35.0	37.0
6	36.05612675	37.0	37.0	37.0	35.0	37.0
7	36.05856925	37.0	37.0	37.0	35.0	37.0
8	36.03507125	37.0	37.0	37.0	35.0	37.0
9	37.7709	39.0	38.0	39.0	35.0	39.0
10-14	38.0191402	39.4	38.2	39.4	35.2	39.4
15-19	39.1599034	41.0	39.0	41.0	36.0	41.0
20-24	38.94221475	41.0	39.0	41.0	35.4	41.0
25-29	38.555566150000004	40.0	38.2	41.0	34.8	41.0
30-34	37.90907885	40.0	37.0	41.0	33.2	41.0
35-39	36.9196723	38.6	35.2	40.8	32.4	41.0
40-44	36.01398715	37.0	35.0	40.0	31.0	41.0
45-49	35.094802900000005	35.0	35.0	38.8	30.8	41.0
50-54	33.81393305	35.0	33.2	36.2	28.8	39.8
55-59	33.92604845	35.0	34.4	35.0	30.0	39.0
60-64	33.6015908	35.0	34.0	35.0	30.0	37.2
65-69	33.2848111	35.0	34.0	35.0	29.4	36.0
70-74	32.998598799999996	35.0	33.8	35.0	29.0	35.0
75-79	32.705027	35.0	33.0	35.0	28.6	35.0
80-84	32.4192671	35.0	33.0	35.0	27.0	35.0
85-89	32.12351099999999	35.0	33.0	35.0	27.0	35.0
90-94	31.749381600000003	35.0	33.0	35.0	25.6	35.0
95-99	31.181856449999998	35.0	33.0	35.0	23.8	35.0
100-101	30.064697625	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10609.0
3	1800.0
4	2036.0
5	2411.0
6	2876.0
7	3677.0
8	4254.0
9	4813.0
10	5165.0
11	5282.0
12	5329.0
13	5174.0
14	5198.0
15	5291.0
16	5772.0
17	6371.0
18	7184.0
19	8141.0
20	9528.0
21	11417.0
22	13930.0
23	16975.0
24	20660.0
25	25467.0
26	30753.0
27	37469.0
28	45227.0
29	56198.0
30	69953.0
31	88767.0
32	116186.0
33	162868.0
34	262897.0
35	627947.0
36	1353974.0
37	723764.0
38	196166.0
39	38251.0
40	220.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.614566899763634	23.01006003577814	19.055188756471832	27.320184307986395
2	30.742673333625376	23.595676906998428	22.084722109228817	23.57692765014738
3	29.30667467677943	21.727631134307064	21.081890887245148	27.883803301668358
4	31.20224851845882	20.520409174692205	19.415498337490025	28.86184396935895
5	30.7626298721211	19.819665339523056	19.452712842586415	29.96499194576943
6	30.16196104070861	19.100942892688117	18.3206515459008	32.41644452070247
7	32.05236111510616	18.930941807226354	19.178740396772888	29.837956680894596
8	29.64355640016832	19.35208994125238	19.824307535116727	31.180046123462574
9	30.392263082421312	18.306794874649913	19.565587821626128	31.735354221302646
10-14	30.563109915617964	19.293153980018342	19.417300960319732	30.726435144043958
15-19	30.64981727727789	19.168135213914077	19.32911106618581	30.852936442622227
20-24	30.61864436656255	19.212847196264136	19.326676339535187	30.841832097638132
25-29	30.70933868906328	19.27930268700088	19.247369527214715	30.763989096721122
30-34	30.779862181759526	19.143881392786987	19.16778889376542	30.908467531688068
35-39	30.715595069554634	19.210837502520967	19.223214172752613	30.850353255171786
40-44	30.779220090876326	19.238560755491964	19.211379461662375	30.770839691969336
45-49	30.85736143786111	19.120617626249114	19.125182820269863	30.89683811561991
50-54	30.692487109605864	19.18666114801629	19.248438428468795	30.872413313909043
55-59	30.76139744477379	19.25588394983475	19.211895399958774	30.77082320543268
60-64	30.809371161874232	19.165908833181767	19.148593829718767	30.87612617522523
65-69	30.687638253477544	19.234514048010823	19.270429139594306	30.807418558917327
70-74	30.75560115112023	19.29772385954477	19.230803846160768	30.71587114317423
75-79	30.80247162654981	19.18410583788047	19.183295835733965	30.830126699835752
80-84	30.69673751267905	19.256276373750058	19.270246704846905	30.776739408723984
85-89	30.77971615594323	19.31486386297277	19.22429884485977	30.681121136224228
90-94	30.83396154169808	19.193150959657547	19.17881095894055	30.79407653970383
95-99	30.714585359390195	19.26456948427113	19.270499528746466	30.750345627592207
100-101	30.851884315074518	19.360854886839093	19.211003688029503	30.576257110056883
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.934751207750026
2	54.31960098377275
3	57.190477978447795
4	60.06409248781777
5	60.72762181789053
6	62.57840556141109
7	61.89031779600076
8	60.823602523630896
9	62.12761730372396
10-14	61.28954505966192
15-19	61.50275371990011
20-24	61.460476464200674
25-29	61.473327785784406
30-34	61.6883297134476
35-39	61.56594832472643
40-44	61.550059782845665
45-49	61.75419955348103
50-54	61.56490042351491
55-59	61.53222065020647
60-64	61.68549733709947
65-69	61.49505681239488
70-74	61.47147229429446
75-79	61.63259832638557
80-84	61.473476921403034
85-89	61.46083729216746
90-94	61.628038081401904
95-99	61.4649309869824
100-101	61.4281414251314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	115.5
2	66.5
3	56.0
4	53.5
5	64.5
6	75.5
7	68.5
8	74.5
9	101.5
10	112.0
11	132.0
12	163.5
13	200.0
14	250.0
15	307.5
16	380.0
17	461.0
18	580.0
19	746.5
20	938.0
21	1208.5
22	1534.0
23	1923.0
24	2386.5
25	2915.5
26	3600.5
27	4426.5
28	5529.0
29	6724.5
30	7924.5
31	9293.0
32	10675.5
33	12280.5
34	13826.0
35	15117.0
36	16531.5
37	17871.5
38	19134.5
39	20397.0
40	21376.0
41	22282.5
42	23376.0
43	24479.5
44	25758.5
45	27426.0
46	29401.0
47	32239.5
48	35807.0
49	40047.0
50	45343.5
51	51600.0
52	58943.5
53	66813.0
54	75761.5
55	85367.0
56	95595.5
57	106633.5
58	117831.0
59	129893.0
60	142231.0
61	154708.0
62	166669.5
63	177648.0
64	187891.0
65	195498.0
66	199366.5
67	199346.0
68	194972.5
69	186193.0
70	173159.0
71	156258.0
72	137108.5
73	115659.5
74	93560.5
75	73023.0
76	54145.0
77	38080.5
78	25078.0
79	15337.5
80	8708.5
81	4622.5
82	2330.5
83	1191.5
84	709.5
85	484.0
86	362.5
87	272.0
88	199.0
89	169.0
90	148.0
91	122.5
92	102.0
93	84.5
94	72.5
95	59.5
96	39.5
97	26.5
98	25.0
99	19.5
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13455
2	0.129375
3	0.12625
4	0.12090000000000001
5	0.13012500000000002
6	0.04695
7	0.049874999999999996
8	0.07255
9	0.086825
10-14	0.033815
15-19	0.03159
20-24	0.012425000000000002
25-29	0.009895000000000001
30-34	0.031375
35-39	0.013495
40-44	0.0047599999999999995
45-49	0.00425
50-54	0.011785
55-59	0.008069999999999999
60-64	1.9999999999999998E-5
65-69	2.55E-4
70-74	1.9999999999999998E-5
75-79	2.65E-4
80-84	0.00237
85-89	1.9999999999999998E-5
90-94	4.9999999999999996E-6
95-99	7.5E-4
100-101	7.999999999999999E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3842041627946426
#Duplication Level	Relative count
1	100.0
2	0.2929006695588936
3	0.008024675878325851
4	0.0010030844847907314
5	0.002006168969581463
6	5.015422423953657E-4
7	0.0015046267271860972
8	0.0
9	0.0
10++	0.0025077112119768286
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	408295	3.8993049	4.363381	1
TTTTT	381015	3.6855862	4.093481	10-14
>>END_MODULE