##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.515234	34.0	31.0	34.0	31.0	34.0
2	32.68806875	34.0	31.0	34.0	31.0	34.0
3	32.71449575	34.0	31.0	34.0	31.0	34.0
4	36.1220135	37.0	37.0	37.0	35.0	37.0
5	36.10937475	37.0	37.0	37.0	35.0	37.0
6	36.11001475	37.0	37.0	37.0	35.0	37.0
7	36.11040525	37.0	37.0	37.0	35.0	37.0
8	36.08392375	37.0	37.0	37.0	35.0	37.0
9	37.82933425	39.0	39.0	39.0	35.0	39.0
10-14	38.10134455	39.4	38.4	39.4	35.4	39.4
15-19	39.2515527	41.0	39.6	41.0	36.2	41.0
20-24	39.03370205	41.0	39.0	41.0	35.6	41.0
25-29	38.64459695000001	40.2	38.4	41.0	35.0	41.0
30-34	37.97528685	40.0	37.2	41.0	33.4	41.0
35-39	36.98237025	38.8	35.2	40.8	32.6	41.0
40-44	36.071483799999996	37.0	35.0	40.0	31.6	41.0
45-49	35.141542400000006	35.0	35.0	39.0	30.8	41.0
50-54	33.830571	34.8	33.2	36.2	28.8	39.8
55-59	33.99434724999999	35.0	34.8	35.2	30.4	39.2
60-64	33.652401350000005	35.0	34.4	35.0	30.0	37.2
65-69	33.303607750000005	35.0	34.0	35.0	29.6	36.0
70-74	33.00798404999999	35.0	33.8	35.0	29.0	35.0
75-79	32.739029599999995	35.0	33.0	35.0	29.0	35.0
80-84	32.41268635	35.0	33.0	35.0	27.0	35.0
85-89	32.09146955	35.0	33.0	35.0	27.0	35.0
90-94	31.697455100000003	35.0	33.0	35.0	25.2	35.0
95-99	31.13900615	35.0	33.0	35.0	23.6	35.0
100-101	29.971491125	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10410.0
3	1761.0
4	1951.0
5	2255.0
6	2921.0
7	3549.0
8	4130.0
9	4665.0
10	4982.0
11	5081.0
12	5117.0
13	5104.0
14	5250.0
15	5329.0
16	5668.0
17	6491.0
18	7149.0
19	8177.0
20	9407.0
21	11273.0
22	13478.0
23	16123.0
24	20024.0
25	24213.0
26	29630.0
27	36024.0
28	43895.0
29	54726.0
30	67851.0
31	86560.0
32	114523.0
33	160861.0
34	266062.0
35	642275.0
36	1355778.0
37	721207.0
38	197249.0
39	38610.0
40	241.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.6423283306405	23.022520631976032	19.00588383188308	27.32926720550039
2	30.717037132011743	23.573285626351964	22.08054760188778	23.629129639748516
3	29.358347235417586	21.73515540368297	21.040184744551393	27.86631261634805
4	31.213525465412996	20.5211393613609	19.426568422201807	28.8387667510243
5	30.73107672616277	19.826006007615053	19.444752550807713	29.99816471541446
6	30.17443613915346	19.04165868883191	18.359156489452364	32.42474868256227
7	32.05920899814174	18.906415200884275	19.148534618095606	29.88584118287838
8	29.600247182019814	19.298976568286285	19.830823537777636	31.269952711916265
9	30.426948813371634	18.271530059557424	19.540659341209714	31.760861785861227
10-14	30.58689401535939	19.262734432818007	19.39162862456103	30.758742927261572
15-19	30.66664191021948	19.15043980812686	19.296971617550724	30.885946664102942
20-24	30.64351430573236	19.127854537391386	19.339545235228233	30.889085921648025
25-29	30.721164123897037	19.237839009615364	19.24721958496032	30.793777281527273
30-34	30.81274710975427	19.121440368349933	19.151158600670637	30.914653921225167
35-39	30.726575296846182	19.171356388831047	19.236695120043844	30.865373194278934
40-44	30.803994529513613	19.222098876225317	19.1657553274391	30.80815126682197
45-49	30.849565473927086	19.09507773210313	19.124889468983937	30.930467324985845
50-54	30.74772969649191	19.17655474785737	19.192984090403005	30.882731465247716
55-59	30.785545618462134	19.218218621557813	19.19579825774464	30.800437502235408
60-64	30.832392790208825	19.149914682367758	19.107959617337407	30.909732910086014
65-69	30.724839999999997	19.20982	19.22314	30.8422
70-74	30.791415	19.264095	19.20127	30.743219999999997
75-79	30.828870015545107	19.18337085600495	19.12964203051257	30.85811709793737
80-84	30.72894683252804	19.234951192570403	19.228274902711203	30.807827072190346
85-89	30.810682782317855	19.268305622099373	19.211944976769985	30.709066618812784
90-94	30.856396706264594	19.170776085842924	19.139740752213086	30.8330864556794
95-99	30.76160069991692	19.220987386513777	19.227343085005042	30.79006882856426
100-101	30.91352859745556	19.323374473876903	19.17225258009254	30.590844348575004
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.971595536140896
2	54.34616677176026
3	57.22465985176564
4	60.052292216437294
5	60.72924144157723
6	62.599184821715724
7	61.94505018102012
8	60.87019989393608
9	62.18781059923286
10-14	61.34563694262096
15-19	61.55258857432242
20-24	61.532600227380385
25-29	61.51494140542432
30-34	61.727401030979436
35-39	61.591948491125116
40-44	61.61214579633558
45-49	61.780032798912934
50-54	61.630461161739625
55-59	61.58598312069754
60-64	61.742125700294835
65-69	61.56703999999999
70-74	61.534635
75-79	61.68698711348248
80-84	61.53677390471839
85-89	61.51974940113064
90-94	61.68948316194399
95-99	61.55166952848118
100-101	61.504372946030564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	52.0
2	32.5
3	30.5
4	31.0
5	36.5
6	45.0
7	53.5
8	69.0
9	81.5
10	107.5
11	145.0
12	167.5
13	211.5
14	269.0
15	317.0
16	413.5
17	504.5
18	602.5
19	766.0
20	961.0
21	1185.0
22	1509.5
23	1950.5
24	2420.5
25	2941.5
26	3648.0
27	4481.5
28	5437.5
29	6644.0
30	7887.0
31	9190.5
32	10682.0
33	12300.0
34	13666.5
35	14986.5
36	16612.0
37	17924.5
38	19051.5
39	20205.5
40	21330.0
41	22428.5
42	23444.5
43	24511.5
44	25886.0
45	27537.5
46	29559.0
47	32229.5
48	35502.0
49	39993.5
50	45316.5
51	51102.0
52	58331.5
53	66883.5
54	75877.5
55	85126.0
56	95266.0
57	106225.5
58	117594.5
59	129907.5
60	142171.0
61	154216.5
62	166383.0
63	177464.5
64	187164.5
65	194174.0
66	197946.0
67	198570.0
68	194830.0
69	186482.5
70	173602.5
71	156969.0
72	137572.5
73	116181.5
74	94731.0
75	73791.0
76	54603.5
77	38384.5
78	25301.0
79	15779.5
80	9174.0
81	4961.5
82	2552.5
83	1393.0
84	882.5
85	602.5
86	448.5
87	353.0
88	294.0
89	255.0
90	209.0
91	173.0
92	142.5
93	116.0
94	96.0
95	76.5
96	72.0
97	68.0
98	50.0
99	29.0
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1671
2	0.16835
3	0.16485
4	0.157225
5	0.151725
6	0.113875
7	0.12160000000000001
8	0.15455
9	0.167775
10-14	0.10411000000000001
15-19	0.09336499999999999
20-24	0.06174
25-29	0.059435
30-34	0.095
35-39	0.06692999999999999
40-44	0.041795
45-49	0.039369999999999995
50-54	0.056865
55-59	0.046225
60-64	1.55E-4
65-69	0.0
70-74	0.0
75-79	0.00712
80-84	0.01932
85-89	0.001145
90-94	0.001075
95-99	0.01099
100-101	0.0144875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3502233235743828
#Duplication Level	Relative count
1	100.0
2	0.2561955709751977
3	0.006517695543400332
4	0.002506805978230897
5	5.013611956461794E-4
6	0.0010027223912923587
7	0.0
8	0.0010027223912923587
9	5.013611956461794E-4
10++	0.004010889565169435
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	389865	3.744558	4.3209786	60-64
TTTTT	370610	3.6139076	4.083575	10-14
>>END_MODULE