##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60965125	34.0	31.0	34.0	31.0	34.0
2	32.776509	34.0	31.0	34.0	31.0	34.0
3	32.8169305	34.0	31.0	34.0	31.0	34.0
4	36.18799225	37.0	37.0	37.0	35.0	37.0
5	36.197846	37.0	37.0	37.0	35.0	37.0
6	36.1898385	37.0	37.0	37.0	35.0	37.0
7	36.1653115	37.0	37.0	37.0	35.0	37.0
8	36.13671975	37.0	37.0	37.0	35.0	37.0
9	37.89706925	39.0	39.0	39.0	37.0	39.0
10-14	38.1922804	39.4	39.2	39.4	36.6	39.4
15-19	39.35807075	41.0	40.0	41.0	37.0	41.0
20-24	39.1757809	41.0	39.4	41.0	36.2	41.0
25-29	38.78504875	40.4	38.6	41.0	35.0	41.0
30-34	38.124087599999996	40.0	37.4	41.0	34.2	41.0
35-39	37.1564877	39.0	35.2	40.8	33.0	41.0
40-44	36.2197369	37.0	35.0	40.0	32.0	41.0
45-49	35.266763649999994	35.0	35.0	39.0	31.2	41.0
50-54	33.940823449999996	34.8	33.4	36.2	29.6	39.8
55-59	34.155062199999996	35.0	35.0	35.2	31.0	39.2
60-64	33.786291649999995	35.0	35.0	35.0	31.0	37.2
65-69	33.46086375	35.0	34.2	35.0	30.2	36.0
70-74	33.174578600000004	35.0	34.0	35.0	29.6	35.0
75-79	32.89737484999999	35.0	33.8	35.0	29.0	35.0
80-84	32.5859722	35.0	33.0	35.0	28.6	35.0
85-89	32.259884899999996	35.0	33.0	35.0	27.0	35.0
90-94	31.889315500000002	35.0	33.0	35.0	26.6	35.0
95-99	31.3684551	35.0	33.0	35.0	24.2	35.0
100-101	30.300643125	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10645.0
3	1755.0
4	2076.0
5	2145.0
6	2627.0
7	3277.0
8	3679.0
9	4135.0
10	4419.0
11	4657.0
12	4790.0
13	4664.0
14	4770.0
15	4960.0
16	5331.0
17	5778.0
18	6366.0
19	7330.0
20	8431.0
21	10237.0
22	12359.0
23	14844.0
24	18138.0
25	22106.0
26	26790.0
27	32837.0
28	40055.0
29	49841.0
30	62838.0
31	79825.0
32	106058.0
33	151366.0
34	253023.0
35	634048.0
36	1393511.0
37	752736.0
38	206240.0
39	41045.0
40	268.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.644164652014283	22.979480590234726	18.987714793323708	27.38863996442728
2	30.7341717644732	23.561617428552474	22.070391664257023	23.633819142717304
3	29.32425470041563	21.734193129740035	21.021751767584593	27.919800402259742
4	31.225211853005113	20.52076228284363	19.397187307968125	28.856838556183128
5	30.76211787999788	19.797492289782074	19.405324730054787	30.035065100165266
6	30.19453937732377	19.01768881210801	18.31108774301774	32.47668406755048
7	32.10474421395632	18.90725775853152	19.102653266638733	29.885344760873416
8	29.635003249504305	19.286497044379157	19.793013841334997	31.28548586478154
9	30.418348700172615	18.25438685947943	19.537363632195117	31.789900808152836
10-14	30.594074959349292	19.242793904329282	19.38812040371332	30.775010732608106
15-19	30.690991867079255	19.109985677472377	19.279585071248167	30.9194373842002
20-24	30.65159653329147	19.136264521917802	19.298275728931575	30.91386321585915
25-29	30.718052489422227	19.23424536852814	19.24541582239739	30.802286319652243
30-34	30.818050408457225	19.09055968750255	19.146386405295562	30.94500349874466
35-39	30.724866143478817	19.145176020374457	19.21573771528849	30.91422012085824
40-44	30.805416087685337	19.172008456926214	19.163571746788808	30.859003708599637
45-49	30.86863972706334	19.076717405340606	19.094518927375745	30.960123940220306
50-54	30.75357448936688	19.158301195398078	19.170956867926723	30.917167447308312
55-59	30.801814357732543	19.203397099587573	19.185118219519993	30.809670323159892
60-64	30.85287785606437	19.127554245728902	19.087698434663146	30.93186946354358
65-69	30.73221	19.200805000000003	19.19859	30.868395
70-74	30.796400000000002	19.251495	19.17514	30.776965
75-79	30.840579350634883	19.147505174666858	19.111226117600257	30.900689357098
80-84	30.72377848481417	19.223880725424138	19.205543441855106	30.84679734790659
85-89	30.792172468868795	19.244873396748094	19.18683812424359	30.77611601013952
90-94	30.879383161373962	19.16272642936722	19.101488388898506	30.856402020360314
95-99	30.758058150638234	19.225420133284178	19.202560840981857	30.813960875095724
100-101	30.891037107944985	19.31971581150222	19.13831836589738	30.650928714655418
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.03280461644157
2	54.3679909071905
3	57.244055102675375
4	60.08205040918825
5	60.79718298016314
6	62.671223444874244
7	61.99008897482974
8	60.92048911428585
9	62.20824950832545
10-14	61.369085691957395
15-19	61.610429251279456
20-24	61.56545974915062
25-29	61.520338809074474
30-34	61.76305390720188
35-39	61.63908626433705
40-44	61.664419796284974
45-49	61.828763667283646
50-54	61.6707419366752
55-59	61.61148468089244
60-64	61.78474731960796
65-69	61.600604999999995
70-74	61.573365
75-79	61.741268707732885
80-84	61.570575832720756
85-89	61.56828847900832
90-94	61.73578518173427
95-99	61.57201902573396
100-101	61.541965822600396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	21.5
2	17.0
3	20.0
4	27.0
5	29.5
6	36.5
7	58.0
8	80.5
9	106.5
10	136.0
11	173.0
12	228.0
13	294.5
14	350.0
15	421.0
16	502.0
17	582.5
18	695.0
19	860.5
20	1035.5
21	1259.0
22	1565.0
23	1928.0
24	2363.0
25	2877.5
26	3567.5
27	4531.0
28	5559.0
29	6526.0
30	7724.0
31	9059.0
32	10576.5
33	12183.0
34	13465.0
35	14872.5
36	16358.5
37	17690.5
38	19130.0
39	20360.5
40	21479.5
41	22369.5
42	23047.0
43	24277.0
44	25691.0
45	27345.0
46	29560.0
47	32178.5
48	35526.5
49	39790.0
50	44981.0
51	51217.5
52	58479.5
53	66451.5
54	75201.5
55	84745.5
56	94927.5
57	105894.0
58	117619.0
59	129684.0
60	141533.0
61	153645.5
62	165998.5
63	177634.0
64	187585.5
65	194802.5
66	198686.0
67	198660.5
68	194651.5
69	186554.5
70	173965.5
71	157142.5
72	137360.0
73	116402.0
74	95010.5
75	73954.0
76	55089.5
77	39064.0
78	25912.5
79	16002.5
80	9167.0
81	4962.0
82	2661.0
83	1472.5
84	911.0
85	653.0
86	499.0
87	384.5
88	322.0
89	295.0
90	252.5
91	211.0
92	182.5
93	140.5
94	106.0
95	97.0
96	89.0
97	70.0
98	46.5
99	35.0
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1482
2	0.140875
3	0.13915
4	0.122375
5	0.1001
6	0.14875000000000002
7	0.176825
8	0.21555
9	0.226175
10-14	0.152415
15-19	0.12936
20-24	0.09024499999999999
25-29	0.093585
30-34	0.128465
35-39	0.09451999999999999
40-44	0.079535
45-49	0.064725
50-54	0.08432999999999999
55-59	0.075935
60-64	0.002035
65-69	0.0
70-74	0.0
75-79	0.024965
80-84	0.03972
85-89	0.009085
90-94	0.004965
95-99	0.04065
100-101	0.0399375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.39758253833474594
#Duplication Level	Relative count
1	100.0
2	0.2773125257003019
3	0.006519100965829881
4	0.003008815830383022
5	0.0020058772202553483
6	5.014693050638371E-4
7	0.0
8	0.0
9	0.0
10++	0.004513223745574534
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	370695	3.6423335	3.9571319	10-14
AAAAA	376920	3.6346042	4.0806293	1
>>END_MODULE