##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.568356	34.0	31.0	34.0	31.0	34.0
2	32.75763475	34.0	31.0	34.0	31.0	34.0
3	32.7807125	34.0	31.0	34.0	31.0	34.0
4	36.183403	37.0	37.0	37.0	35.0	37.0
5	36.17399025	37.0	37.0	37.0	35.0	37.0
6	36.149404	37.0	37.0	37.0	35.0	37.0
7	36.1471185	37.0	37.0	37.0	35.0	37.0
8	36.116996	37.0	37.0	37.0	35.0	37.0
9	37.87712625	39.0	39.0	39.0	35.0	39.0
10-14	38.1662058	39.4	39.0	39.4	35.4	39.4
15-19	39.311397750000005	41.0	40.0	41.0	36.6	41.0
20-24	39.104297700000004	41.0	39.0	41.0	35.8	41.0
25-29	38.7127627	40.4	38.4	41.0	35.0	41.0
30-34	38.044365049999996	40.0	37.2	41.0	33.8	41.0
35-39	37.06185240000001	38.8	35.2	40.8	32.8	41.0
40-44	36.1214624	37.0	35.0	40.0	31.8	41.0
45-49	35.16768235	35.0	35.0	39.0	31.0	41.0
50-54	33.83991925	34.8	33.2	36.2	28.8	39.8
55-59	34.05476755	35.0	35.0	35.2	31.0	39.2
60-64	33.68258805	35.0	34.6	35.0	30.6	37.2
65-69	33.31473395	35.0	34.0	35.0	29.6	36.0
70-74	33.00834265	35.0	34.0	35.0	29.0	35.0
75-79	32.697837	35.0	33.0	35.0	28.6	35.0
80-84	32.3663749	35.0	33.0	35.0	27.0	35.0
85-89	32.0235771	35.0	33.0	35.0	26.8	35.0
90-94	31.603469150000002	35.0	33.0	35.0	24.4	35.0
95-99	31.01102155	35.0	32.6	35.0	23.0	35.0
100-101	29.852217500000002	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9611.0
3	1731.0
4	2153.0
5	2271.0
6	2896.0
7	3465.0
8	3979.0
9	4509.0
10	4666.0
11	5059.0
12	5078.0
13	5236.0
14	5159.0
15	5305.0
16	5758.0
17	6362.0
18	7360.0
19	8183.0
20	9461.0
21	11452.0
22	13320.0
23	16494.0
24	19388.0
25	24154.0
26	29147.0
27	34934.0
28	43590.0
29	53644.0
30	67584.0
31	86392.0
32	115041.0
33	163776.0
34	268925.0
35	643912.0
36	1350572.0
37	722903.0
38	197518.0
39	38765.0
40	247.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.62113597246127	22.945166640588326	19.056022531685183	27.377674855265216
2	30.78873981769262	23.532632810773567	22.113159911997133	23.565467459536677
3	29.346020544774344	21.75954356067715	21.004942256922288	27.889493637626217
4	31.18201439316943	20.52037497704309	19.42534913269169	28.872261497095796
5	30.77770653998017	19.827823081346725	19.39156247513142	30.002907903541686
6	30.157022757182606	19.063321895650674	18.318445316459243	32.46121003070747
7	32.03514146088345	18.907981181622983	19.130664307941814	29.92621304955175
8	29.712817119314682	19.284926925019967	19.78678751863161	31.21546843703374
9	30.393060077994488	18.294748856789482	19.53340533620944	31.778785729006582
10-14	30.597454408483472	19.25602954297735	19.394854506342703	30.75166154219648
15-19	30.685772921298383	19.117486423150954	19.29479494533358	30.901945710217078
20-24	30.618135609603996	19.154274681885745	19.328599352654177	30.898990355856082
25-29	30.738788668948676	19.23892825088327	19.232419430205532	30.78986364996253
30-34	30.81021549931304	19.10895167661986	19.13402003657108	30.946812787496032
35-39	30.719811024554843	19.17817813471094	19.223953191977287	30.87805764875693
40-44	30.81651633921572	19.194155890182735	19.152472418152104	30.836855352449437
45-49	30.84697390271488	19.095024738426154	19.10638441122756	30.95161694763141
50-54	30.736144783718476	19.170392886957956	19.18008779646013	30.91337453286344
55-59	30.7841341789026	19.22188468340134	19.175379841244126	30.81860129645193
60-64	30.84520514815004	19.146127334948304	19.104762254286396	30.90390526261526
65-69	30.748710000000003	19.185215	19.19604	30.870035
70-74	30.79383	19.24464	19.16131	30.80022
75-79	30.827998270590566	19.155664751001346	19.143628293386328	30.872708685021756
80-84	30.73331196695333	19.224185341743045	19.217428324097575	30.825074367206046
85-89	30.812692455482583	19.27675114020147	19.17948494869062	30.73107145562533
90-94	30.878221543911078	19.16355095817755	19.095635954781798	30.862591543129575
95-99	30.77865918030232	19.212453329422903	19.205571401106592	30.803316089168188
100-101	30.94654938227151	19.29628862803463	19.126153239873894	30.63100874981996
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.99881082772649
2	54.35420727722929
3	57.23551418240056
4	60.05427589026522
5	60.780614443521856
6	62.61823278789008
7	61.96135451043521
8	60.92828555634843
9	62.17184580700107
10-14	61.349115950679945
15-19	61.587718631515465
20-24	61.51712596546008
25-29	61.528652318911206
30-34	61.75702828680907
35-39	61.59786867331177
40-44	61.65337169166516
45-49	61.798590850346294
50-54	61.649519316581916
55-59	61.60273547535453
60-64	61.7491104107653
65-69	61.618745
70-74	61.59405
75-79	61.700706955612326
80-84	61.55838633415938
85-89	61.54376391110791
90-94	61.74081308704066
95-99	61.581975269470504
100-101	61.57755813209147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	21.5
2	23.5
3	22.0
4	23.5
5	25.0
6	31.5
7	45.5
8	57.0
9	75.0
10	102.0
11	138.5
12	163.0
13	202.5
14	272.5
15	345.0
16	445.5
17	536.0
18	634.5
19	776.5
20	972.5
21	1205.5
22	1494.0
23	1871.0
24	2329.5
25	2935.5
26	3560.5
27	4349.5
28	5427.0
29	6578.0
30	7813.0
31	9107.0
32	10438.5
33	12082.5
34	13659.0
35	15054.5
36	16430.5
37	17607.5
38	18950.5
39	20191.0
40	21184.5
41	22255.5
42	23267.5
43	24358.0
44	25686.5
45	27210.5
46	29351.0
47	32070.0
48	35530.5
49	39662.5
50	44963.0
51	51576.5
52	58708.5
53	66979.5
54	75650.0
55	85072.5
56	95659.0
57	106514.5
58	118152.0
59	130179.0
60	142150.0
61	154805.0
62	166868.0
63	177646.0
64	187082.5
65	194413.0
66	198789.0
67	199196.5
68	194971.0
69	186181.5
70	173037.5
71	156097.5
72	136849.0
73	115780.0
74	94070.5
75	73338.5
76	54739.0
77	38710.0
78	25707.0
79	16074.5
80	9352.5
81	5153.0
82	2820.5
83	1544.0
84	970.0
85	700.5
86	543.5
87	451.5
88	335.5
89	260.0
90	236.0
91	220.0
92	191.0
93	155.5
94	134.5
95	101.0
96	83.0
97	76.5
98	53.5
99	37.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.140625
2	0.105525
3	0.09625
4	0.08455
5	0.09985000000000001
6	0.15460000000000002
7	0.17205
8	0.186425
9	0.166025
10-14	0.111625
15-19	0.075305
20-24	0.06578
25-29	0.058699999999999995
30-34	0.093185
35-39	0.05474
40-44	0.044315
45-49	0.041135
50-54	0.05064
55-59	0.06417
60-64	1.95E-4
65-69	0.0
70-74	0.0
75-79	0.012110000000000001
80-84	0.029859999999999998
85-89	0.001225
90-94	4.9999999999999996E-6
95-99	0.028019999999999996
100-101	0.0208
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.338817038799054
#Duplication Level	Relative count
1	100.0
2	0.276287419144562
3	0.0050142907285764425
4	0.002005716291430577
5	5.014290728576442E-4
6	0.0
7	0.0
8	5.014290728576442E-4
9	0.0
10++	0.001504287218572933
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	380140	3.6630914	4.115513	60-64
TTTTT	372005	3.636909	3.947126	10-14
>>END_MODULE